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22 pages, 1788 KiB  
Article
Multi-Market Coupling Mechanism of Offshore Wind Power with Energy Storage Participating in Electricity, Carbon, and Green Certificates
by Wenchuan Meng, Zaimin Yang, Jingyi Yu, Xin Lin, Ming Yu and Yankun Zhu
Energies 2025, 18(15), 4086; https://doi.org/10.3390/en18154086 (registering DOI) - 1 Aug 2025
Abstract
With the support of the dual-carbon strategy and related policies, China’s offshore wind power has experienced rapid development. However, constrained by the inherent intermittency and volatility of wind power, large-scale expansion poses significant challenges to grid integration and exacerbates government fiscal burdens. To [...] Read more.
With the support of the dual-carbon strategy and related policies, China’s offshore wind power has experienced rapid development. However, constrained by the inherent intermittency and volatility of wind power, large-scale expansion poses significant challenges to grid integration and exacerbates government fiscal burdens. To address these critical issues, this paper proposes a multi-market coupling trading model integrating energy storage-equipped offshore wind power into electricity–carbon–green certificate markets for large-scale grid networks. Firstly, a day-ahead electricity market optimization model that incorporates energy storage is established to maximize power revenue by coordinating offshore wind power generation, thermal power dispatch, and energy storage charging/discharging strategies. Subsequently, carbon market and green certificate market optimization models are developed to quantify Chinese Certified Emission Reduction (CCER) volume, carbon quotas, carbon emissions, market revenues, green certificate quantities, pricing mechanisms, and associated economic benefits. To validate the model’s effectiveness, a gradient ascent-optimized game-theoretic model and a double auction mechanism are introduced as benchmark comparisons. The simulation results demonstrate that the proposed model increases market revenues by 17.13% and 36.18%, respectively, compared to the two benchmark models. It not only improves wind power penetration and comprehensive profitability but also effectively alleviates government subsidy pressures through coordinated carbon–green certificate trading mechanisms. Full article
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22 pages, 3360 KiB  
Article
Effect of Atmospheric Cold Plasma Treatment on the Microorganism Growth, Diversity, and Quality of Coconut Water During Refrigerator Storage
by Lixian Zeng, Wenyue Gu, Yuanyuan Wang, Wentao Deng, Jiamei Wang and Liming Zhang
Foods 2025, 14(15), 2709; https://doi.org/10.3390/foods14152709 (registering DOI) - 1 Aug 2025
Abstract
To study the effect of cold plasma (CP) on the refrigerator shelf life of coconut water, microorganism growth and diversity and physicochemical properties were investigated. Results indicated that CP treatment did not cause significant color changes in coconut water, with turbidity remaining lower [...] Read more.
To study the effect of cold plasma (CP) on the refrigerator shelf life of coconut water, microorganism growth and diversity and physicochemical properties were investigated. Results indicated that CP treatment did not cause significant color changes in coconut water, with turbidity remaining lower than the control even after 6 days of storage. Enzymatic activity analysis revealed reduced polyphenol oxidase (PPO) and peroxidase (POD) levels in treated samples. Specifically, the 12 s CP treatment resulted in the lowest antioxidant capacity values: 15.77 Fe2+/g for ferric reducing antioxidant power (FRAP), 37.15% for DPPH radical scavenging, and 39.51% for ABTS+ radical scavenging. Microbial enumeration showed that extended CP treatment effectively inhibited the growth of total viable counts, psychrophilic bacteria, lactic acid bacteria, and yeast. High-throughput sequencing identified Leuconostoc, Carnobacterium, and Lactobacillus as the dominant bacterial genera. During storage, Carnobacterium was the primary genus in the early stage, while Leuconostoc emerged as the dominant genus by the end of the storage period. In summary, CP as an effective non-thermal technology was able to maintain quality and antioxidant capacity, inhibit microbial growth, and delay the spoilage in coconut water to help extend the refrigerated shelf life of the product. Full article
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28 pages, 4460 KiB  
Article
New Protocol for Hydrogen Refueling Station Operation
by Carlos Armenta-Déu
Future Transp. 2025, 5(3), 96; https://doi.org/10.3390/futuretransp5030096 (registering DOI) - 1 Aug 2025
Abstract
This work proposes a new method to refill fuel cell electric vehicle hydrogen tanks from a storage system in hydrogen refueling stations. The new method uses the storage tanks in cascade to supply hydrogen to the refueling station dispensers. This method reduces the [...] Read more.
This work proposes a new method to refill fuel cell electric vehicle hydrogen tanks from a storage system in hydrogen refueling stations. The new method uses the storage tanks in cascade to supply hydrogen to the refueling station dispensers. This method reduces the hydrogen compressor power requirement and the energy consumption for refilling the vehicle tank; therefore, the proposed alternative design for hydrogen refueling stations is feasible and compatible with low-intensity renewable energy sources like solar photovoltaic, wind farms, or micro-hydro plants. Additionally, the cascade method supplies higher pressure to the dispenser throughout the day, thus reducing the refueling time for specific vehicle driving ranges. The simulation shows that the energy saving using the cascade method achieves 9% to 45%, depending on the vehicle attendance. The hydrogen refueling station design supports a daily vehicle attendance of 9 to 36 with a complete refueling process coverage. The carried-out simulation proves that the vehicle tank achieves the maximum attainable pressure of 700 bars with a storage system of six tanks. The data analysis shows that the daily hourly hydrogen demand follows a sinusoidal function, providing a practical tool to predict the hydrogen demand for any vehicle attendance, allowing the planners and station designers to resize the elements to fulfill the new requirements. The proposed system is also applicable to hydrogen ICE vehicles. Full article
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42 pages, 4775 KiB  
Article
Optimal Sizing of Battery Energy Storage System for Implicit Flexibility in Multi-Energy Microgrids
by Andrea Scrocca, Maurizio Delfanti and Filippo Bovera
Appl. Sci. 2025, 15(15), 8529; https://doi.org/10.3390/app15158529 (registering DOI) - 31 Jul 2025
Abstract
In the context of urban decarbonization, multi-energy microgrids (MEMGs) are gaining increasing relevance due to their ability to enhance synergies across multiple energy vectors. This study presents a block-based MILP framework developed to optimize the operations of a real MEMG, with a particular [...] Read more.
In the context of urban decarbonization, multi-energy microgrids (MEMGs) are gaining increasing relevance due to their ability to enhance synergies across multiple energy vectors. This study presents a block-based MILP framework developed to optimize the operations of a real MEMG, with a particular focus on accurately modeling the structure of electricity and natural gas bills. The objective is to assess the added economic value of integrating a battery energy storage system (BESS) under the assumption it is employed to provide implicit flexibility—namely, bill management, energy arbitrage, and peak shaving. Results show that under assumed market conditions, tariff schemes, and BESS costs, none of the analyzed BESS configurations achieve a positive net present value. However, a 2 MW/4 MWh BESS yields a 3.8% reduction in annual operating costs compared to the base case without storage, driven by increased self-consumption (+2.8%), reduced thermal energy waste (–6.4%), and a substantial decrease in power-based electricity charges (–77.9%). The performed sensitivity analyses indicate that even with a significantly higher day-ahead market price spread, the BESS is not sufficiently incentivized to perform pure energy arbitrage and that the effectiveness of a time-of-use power-based tariff depends not only on the level of price differentiation but also on the BESS size. Overall, this study provides insights into the role of BESS in MEMGs and highlights the need for electricity bill designs that better reward the provision of implicit flexibility by storage systems. Full article
(This article belongs to the Special Issue Innovative Approaches to Optimize Future Multi-Energy Systems)
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15 pages, 522 KiB  
Article
High Humidity Storage Close to Saturation Reduces Kiwifruit Postharvest Rots and Maintains Quality
by Fabio Buonsenso, Simona Prencipe, Silvia Valente, Giulia Remolif, Jean de Barbeyrac, Alberto Sardo and Davide Spadaro
Horticulturae 2025, 11(8), 883; https://doi.org/10.3390/horticulturae11080883 (registering DOI) - 31 Jul 2025
Abstract
Postharvest storage of kiwifruit requires the implementation of precise environmental conditions to maintain fruit quality and reduce decay. In this research, conducted over two years, we examined whether the storage conditions, characterized by low temperature (1 ± 1 °C) and ultra-high relative humidity [...] Read more.
Postharvest storage of kiwifruit requires the implementation of precise environmental conditions to maintain fruit quality and reduce decay. In this research, conducted over two years, we examined whether the storage conditions, characterized by low temperature (1 ± 1 °C) and ultra-high relative humidity (higher than 99%, close to saturation), generated by the Xedavap® machine from Xeda International, were effective in maintaining the fruit quality and reducing postharvest rots compared to standard storage conditions, characterized by involved low temperature (1 ± 1 °C) and high relative humidity (98%). Kiwifruits preserved under the experimental conditions exhibited a significantly lower rot incidence after 60 days of storage, with the treated fruits showing 4.48% rot compared to 23.03% under the standard conditions in the first year, using inoculated fruits, and 6.30% versus 9.20% in the second year using naturally infected fruits, respectively. After shelf life (second year only), rot incidence remained significantly lower in the treated fruits (12.80%) compared to the control (42.30%). Additionally, quality analyses showed better parameters when using the Xedavap® system over standard methods. The ripening process was effectively slowed down, as indicated by changes in the total soluble solids, firmness, and titratable acidity compared to the control. These results highlight the potential of ultra-high relative humidity conditions to reduce postharvest rot, extend the shelf life, and enhance the marketability of kiwifruit, presenting a promising and innovative solution for the horticultural industry. Full article
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17 pages, 2562 KiB  
Article
Comparative Stability and Anesthetic Evaluation of Holy Basil Essential Oil Formulated in SNEDDS and Microemulsion Systems in Cyprinus carpio var. Koi
by Kantaporn Kheawfu, Chuda Chittasupho, Surachai Pikulkaew, Wasana Chaisri and Taepin Junmahasathien
Pharmaceutics 2025, 17(8), 997; https://doi.org/10.3390/pharmaceutics17080997 (registering DOI) - 31 Jul 2025
Abstract
Background/Objectives: Holy basil (Ocimum tenuiflorum L.) essential oil exhibits antioxidant, antimicrobial, and anesthetic activities, mainly due to eugenol, methyl eugenol, and β-caryophyllene. However, its clinical application is limited by poor water solubility, instability, and low bioavailability. This study developed and compared two [...] Read more.
Background/Objectives: Holy basil (Ocimum tenuiflorum L.) essential oil exhibits antioxidant, antimicrobial, and anesthetic activities, mainly due to eugenol, methyl eugenol, and β-caryophyllene. However, its clinical application is limited by poor water solubility, instability, and low bioavailability. This study developed and compared two delivery systems, self-nanoemulsifying drug delivery systems (SNEDDS) and microemulsions (ME), to enhance their stability and fish anesthetic efficacy. Methods: The optimized SNEDDS (25% basil oil, 8.33% coconut oil, 54.76% Tween 80, 11.91% PEG 400) and ME (12% basil oil, 32% Tween 80, 4% sorbitol, 12% ethanol, 40% water) were characterized for droplet size, PDI, zeta potential, pH, and viscosity. Stability was evaluated by monitoring droplet size and PDI over time and by determining the retention of eugenol, methyl eugenol, and β-caryophyllene after storage at 45 °C. Fish anesthetic efficacy was tested in koi carp (Cyprinus carpio var. koi). Results: SNEDDS maintained a small droplet size (~22.78 ± 1.99 nm) and low PDI (0.188 ± 0.088 at day 60), while ME showed significant size enlargement (up to 177.10 ± 47.50 nm) and high PDI (>0.5). After 90 days at 45 °C, SNEDDS retained 94.45% eugenol, 94.08% methyl eugenol, and 88.55% β-caryophyllene, while ME preserved 104.76%, 103.53%, and 94.47%, respectively. In vivo testing showed that SNEDDS achieved faster anesthesia (114.70 ± 24.80 s at 120 ppm) and shorter recovery (379.60 ± 15.61 s) than ME (134.90 ± 4.70 s; 473.80 ± 16.94 s). Ethanol failed to induce anesthesia at 40 ppm and performed poorly compared to SNEDDS and ME at other concentrations (p < 0.0001). Conclusions: SNEDDS demonstrated superior physical stability and fish anesthetic performance compared to ME. These findings support SNEDDS as a promising formulation for delivering holy basil essential oil in biomedical and aquaculture applications. Full article
(This article belongs to the Special Issue Applications of Nanotechnology in Veterinary Drug Delivery)
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23 pages, 4510 KiB  
Article
Identification and Characterization of Biosecurity Breaches on Poultry Farms with a Recent History of Highly Pathogenic Avian Influenza Virus Infection Determined by Video Camera Monitoring in the Netherlands
by Armin R. W. Elbers and José L. Gonzales
Pathogens 2025, 14(8), 751; https://doi.org/10.3390/pathogens14080751 - 30 Jul 2025
Abstract
Biosecurity measures applied on poultry farms, with a recent history of highly pathogenic avian influenza virus infection, were monitored using 24 h/7 days-per-week video monitoring. Definition of biosecurity breaches were based on internationally acknowledged norms. Farms of four different production types (two broiler, [...] Read more.
Biosecurity measures applied on poultry farms, with a recent history of highly pathogenic avian influenza virus infection, were monitored using 24 h/7 days-per-week video monitoring. Definition of biosecurity breaches were based on internationally acknowledged norms. Farms of four different production types (two broiler, two layer, two breeder broiler, and one duck farm) were selected. Observations of entry to and exit from the anteroom revealed a high degree of biosecurity breaches in six poultry farms and good biosecurity practices in one farm in strictly maintaining the separation between clean and potentially contaminated areas in the anteroom. Hand washing with soap and water and/or using disinfectant lotion was rarely observed at entry to the anteroom and was almost absent at exit. Egg transporters did not disinfect fork-lift wheels when entering the egg-storage room nor change or properly disinfect footwear. The egg-storage room was not cleaned and disinfected after egg transport by the farmer. Similarly, footwear and trolley wheels were not disinfected when introducing young broilers or ducklings to the poultry unit. Biosecurity breaches were observed when introducing bedding material in the duck farm. This study shows a need for an engaging awareness and training campaign for poultry farmers and their co-workers as well as for transporters to promote good biosecurity practices. Full article
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15 pages, 2263 KiB  
Article
Comparison of the Trueness of Complete Dentures Fabricated Using Liquid Crystal Display 3D Printing According to Build Angle and Natural Light Exposure
by Haeri Kim, KeunBaDa Son, So-Yeun Kim and Kyu-Bok Lee
J. Funct. Biomater. 2025, 16(8), 277; https://doi.org/10.3390/jfb16080277 - 30 Jul 2025
Abstract
The dimensional accuracy of the intaglio surface of complete dentures fabricated using liquid crystal display (LCD) three-dimensional (3D) printing might be influenced by the build angle and post-processing storage conditions. This study evaluated the effect of build angle and natural light exposure duration [...] Read more.
The dimensional accuracy of the intaglio surface of complete dentures fabricated using liquid crystal display (LCD) three-dimensional (3D) printing might be influenced by the build angle and post-processing storage conditions. This study evaluated the effect of build angle and natural light exposure duration on the intaglio surface trueness of maxillary complete denture bases. Standardized denture base designs (2 mm uniform thickness) were fabricated using an LCD 3D printer (Lilivis Print; Huvitz, Seoul, Republic of Korea) at build angles of 0°, 45°, and 90° (n = 7 per group). All specimens were printed using the same photopolymer resin (Tera Harz Denture; Graphy, Seoul, Republic of Korea) and identical printing parameters, followed by ultrasonic cleaning and ultraviolet post-curing. Specimens were stored under controlled light-emitting diode lighting and exposed to natural light (400–800 lux) for 0, 14, or 30 days. The intaglio surfaces were scanned and superimposed on the original design data, following the International Organization for Standardization 12836. Quantitative assessment included root mean square deviation, mean deviation, and tolerance percentage. Statistical analyses were performed using one-way analysis of variance and paired t-tests (α = 0.05). Build angle and light exposure duration significantly affected surface trueness (p < 0.05). The 90° build angle group exhibited the highest accuracy and dimensional stability, while the 0° group showed the greatest deviations (p < 0.05). These findings underscore the importance of optimizing build orientation and storage conditions in denture 3D printing. Full article
(This article belongs to the Special Issue Bio-Additive Manufacturing in Materials Science)
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22 pages, 2015 KiB  
Article
Enhancing Fucoxanthin Pickering Emulsion Stability and Encapsulation with Seaweed Cellulose Nanofibrils Using High-Pressure Homogenization
by Ying Tuo, Mingrui Wang, Yiwei Yu, Yixiao Li, Xingyuan Hu, Long Wu, Zongpei Zhang, Hui Zhou and Xiang Li
Mar. Drugs 2025, 23(8), 311; https://doi.org/10.3390/md23080311 - 30 Jul 2025
Abstract
Poor solubility and bioavailability have limited the application of fucoxanthin in drug and functional food processing. In order to encapsulate fucoxanthin in delivery systems, in this study, cellulose was isolated from industrial brown algae residues and high-pressure homogenized into cellulose nanofibrils (CNFs). Then, [...] Read more.
Poor solubility and bioavailability have limited the application of fucoxanthin in drug and functional food processing. In order to encapsulate fucoxanthin in delivery systems, in this study, cellulose was isolated from industrial brown algae residues and high-pressure homogenized into cellulose nanofibrils (CNFs). Then, fucoxanthin was encapsulated into the Pickering emulsion stabilized by the CNFs. The effect of high-pressure homogenization on the characteristics of cellulose and the stability of fucoxanthin emulsion was evaluated. The results indicated that CNFs prepared at 105 MPa had a diameter of 87 nm and exhibited high zeta potential and thermal stability. Encapsulation efficiency peaked at 70.8% with 1.0 mg/mL fucoxanthin, and after three freeze–thaw cycles the encapsulation efficiency was higher than 60%. The DPPH scavenging activity after 12 days’ storage at 4 °C was still 42%. Furthermore, the Pickering emulsion with 1.0 mg/mL fucoxanthin showed high stability and antioxidant activity under different pH values, salinity, temperature, and UV light exposure duration. The CNFs effectively protected fucoxanthin from degradation, offering a novel delivery system for marine bioactive compounds. To the best of our knowledge, this is the first study on the fucoxanthin delivery system of Pickering emulsion stabilized by the CNFs. Such emulsion might benefit the encapsulation and release of bioactive components in marine drugs. Full article
(This article belongs to the Special Issue Marine Carotenoids: Properties, Health Benefits, and Applications)
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26 pages, 4184 KiB  
Article
Effects of Partial Freezing and Superchilling Storage on the Quality of Beef: A Kinetic Modelling Approach
by Anjelina William Mwakosya, Graciela Alvarez and Fatou Toutie Ndoye
Foods 2025, 14(15), 2687; https://doi.org/10.3390/foods14152687 - 30 Jul 2025
Abstract
The current study explores the changes in beef quality following partial freezing and during superchilled storage, alongside chilled storage comparisons. Kinetic models were developed to predict changes in colour difference (∆E), thiobarbituric acid−reactive substances (TBARS), total volatile basic nitrogen (TVB−N), drip loss and [...] Read more.
The current study explores the changes in beef quality following partial freezing and during superchilled storage, alongside chilled storage comparisons. Kinetic models were developed to predict changes in colour difference (∆E), thiobarbituric acid−reactive substances (TBARS), total volatile basic nitrogen (TVB−N), drip loss and firmness. Beef samples were partially frozen in an air blast freezer at −30 °C for 9 min prior to storage at −5 °C, −4 °C, −2.8 °C, −1.8 °C. Chilled beef samples were directly stored at 2 °C and 6 °C without partial freezing. All samples were stored for 21 days. The lightness (L*), redness (a*), yellowness (b*) and colour difference (∆E) were significantly lower in superchilled storage samples compared to chilled storage samples. The pH of beef samples increased gradually over time (p < 0.05). TBARS, TVB−N and drip loss increased while firmness decreased with the increase in storage time in both storage conditions (p < 0.05). Overall, beef quality was affected by both storage duration and temperature. Firmness followed the first order kinetic model; drip loss, TVB−N, TBARS and colour difference (∆E) fitted the zero−order kinetic model. Temperature dependence was adequately modelled using Arrhenius−type equation with the activation energy values of 110.111, 52.870, 68.553, 119.480, 47.301 kJ/mol for drip loss, firmness, TBARS, TVB−N and colour difference (∆E), respectively. The models demonstrated strong predictive performance, with RMSE and MAPE values within ±10%. The developed kinetic models successfully predicted quality changes within the −5 °C to 6 °C temperature range. Full article
(This article belongs to the Section Food Engineering and Technology)
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18 pages, 2433 KiB  
Article
Effect of Preharvest Aluminum-Coated Paper Bagging on Postharvest Quality, Storability, and Browning Behavior of ‘Afrata Volou’ Quince
by Triantafyllia Georgoudaki, Persefoni Maletsika and George D. Nanos
Horticulturae 2025, 11(8), 881; https://doi.org/10.3390/horticulturae11080881 - 30 Jul 2025
Abstract
As consumer preferences tend toward safer, chemical residue-free, and nutritionally rich fruits, preharvest bagging has gained attention as a sustainable method for improving fruit quality and protecting produce from environmental and biological stressors and pesticide residues. This study assessed the impact of preharvest [...] Read more.
As consumer preferences tend toward safer, chemical residue-free, and nutritionally rich fruits, preharvest bagging has gained attention as a sustainable method for improving fruit quality and protecting produce from environmental and biological stressors and pesticide residues. This study assessed the impact of preharvest bagging using paper bags with inner aluminum coating on the physicochemical traits, storability, and browning susceptibility after cutting or bruising of ‘Afrata Volou’ quince (Cydonia oblonga Mill.) fruit grown in central Greece. Fruits were either bagged or left unbagged approximately 60 days before harvest, and evaluations were conducted at harvest and after three months of cold storage, plus two days of shelf-life. Fruit bagging reduced the quince’s flesh temperature on the tree crown. Bagging had minor effects on fruit and nutritional quality, except for more yellow skin and higher titratable acidity (TA). Also, at harvest, bagging did not significantly affect fruit flesh browning after cutting or bruising. After three months of storage, unbagged and bagged quince fruit developed more yellow skin color, without significant alterations in most quality characteristics and nutritional value, but increased total tannin content (TTC). After three months of storage, the quince flesh color determined immediately after cutting or bruising was brighter and more yellowish compared to that at harvest, due to continuation of fruit ripening, but it darkened faster with time after cutting or skin removal. Therefore, fruit bagging appears to be a sustainable practice for improving the aesthetic and some chemical quality characteristics of quince, particularly after storage, without negative impacts on other characteristics such as texture and phenolic content. Full article
(This article belongs to the Special Issue Advances in Tree Crop Cultivation and Fruit Quality Assessment)
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24 pages, 3325 KiB  
Article
Multi-Energy Flow Optimal Dispatch of a Building Integrated Energy System Based on Thermal Comfort and Network Flexibility
by Jian Sun, Bingrui Sun, Xiaolong Cai, Dingqun Liu and Yongping Yang
Energies 2025, 18(15), 4051; https://doi.org/10.3390/en18154051 - 30 Jul 2025
Viewed by 17
Abstract
An efficient integrated energy system (IES) can enhance the potential of building energy conservation and carbon mitigation. However, imbalances between user-side demand and supply side output present formidable challenges to the operational dispatch of building energy systems. To mitigate heat rejection and improve [...] Read more.
An efficient integrated energy system (IES) can enhance the potential of building energy conservation and carbon mitigation. However, imbalances between user-side demand and supply side output present formidable challenges to the operational dispatch of building energy systems. To mitigate heat rejection and improve dispatch optimization, an integrated building energy system incorporating waste heat recovery via an absorption heat pump based on the flow temperature model is adopted. A comprehensive analysis was conducted to investigate the correlation among heat pump operational strategies, thermal comfort, and the dynamic thermal storage capacity of piping network systems. The optimization calculations and comparative analyses were conducted across five cases on typical season days via the CPLEX solver with MATLAB R2018a. The simulation results indicate that the operational modes of absorption heat pump reduced the costs by 4.4–8.5%, while the absorption rate of waste heat increased from 37.02% to 51.46%. Additionally, the utilization ratio of battery and thermal storage units decreased by up to 69.82% at most after considering the pipeline thermal inertia and thermal comfort, thus increasing the system’s energy-saving ability and reducing the pressure of energy storage equipment, ultimately increasing the scheduling flexibility of the integrated building energy system. Full article
(This article belongs to the Special Issue Energy Efficiency and Thermal Performance in Buildings)
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16 pages, 1583 KiB  
Article
The Influence of Ultraviolet-C Light Pretreatment on Blackcurrant (Ribes nigrum) Quality During Storage
by Zhuoyu Wang, Andrej Svyantek, Zachariah Miller, Haydon Davis and Ashley Kapus
Appl. Sci. 2025, 15(15), 8452; https://doi.org/10.3390/app15158452 - 30 Jul 2025
Viewed by 46
Abstract
Blackcurrant is a notable superfruit in Europe, and its vitamin C content surpasses the well-known blueberry superfruit. However, due to its short shelf life during storage, consumption is mainly accounted by frozen berries, extracts, and concentrates. This study applied an intensity of 1.2 [...] Read more.
Blackcurrant is a notable superfruit in Europe, and its vitamin C content surpasses the well-known blueberry superfruit. However, due to its short shelf life during storage, consumption is mainly accounted by frozen berries, extracts, and concentrates. This study applied an intensity of 1.2 W/m2 UVC with different durations, including control (non-treated), UVC irradiation for 0.5 h (0.5 h treatment), UVC irradiation for 1 h (1 h treatment), and UVC pretreatment for 2 h (2 h treatment) to blackcurrant berries before storage. Fundamental physical (firmness and weight loss) and physicochemical characteristics (SSC, pH, and acids), microbial population changes, total phenolic content, antioxidant capacity, and specific phenolic compound changes were evaluated every five days over a twenty-day storage period. The results indicated that the longer the UVC pretreatment, the lower the water weight losses during storage. Meanwhile, the UVC pretreatment significantly affected the blackcurrant soluble solid content, resulting in higher soluble solid contents detected in the blackcurrants with the higher doses of UVC. For the mold population control, UVC effects were highly correlated with the pretreatment duration. However, UVC did not have a significant influence on the berry pH and acid contents, but the storage length slightly increased the pH and decreased the acids. At the same time, UVC pretreatment did not affect the berry firmness, polyphenols, ascorbic acid content, or antioxidant capacities, which were primarily influenced by the storage duration. The monophenolic compounds detected before and after storage indicated that more than one hour of UVC radiation influenced most of the phenolic contents largely before storage. The UVC pretreatment has also influenced some phenolic compounds. After storage, half an hour of UVC pretreatment increased cyanidin levels, and two hours of UVC pretreatment increased catechin and epicatechin levels. However, most of the compounds remained at similar amounts during storage in each treatment. Further research is needed to improve the UVC radiation time length or intensity or explore other technology combinations to optimize UVC pretreatments for blackcurrant storage. Full article
(This article belongs to the Section Food Science and Technology)
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16 pages, 2078 KiB  
Article
Optimizing Yeast Surface-Displayed Unspecific Peroxygenase Production for Sustainable Biocatalysis
by Niklas Teetz, Luc Zuhse and Dirk Holtmann
Bioengineering 2025, 12(8), 822; https://doi.org/10.3390/bioengineering12080822 - 30 Jul 2025
Viewed by 54
Abstract
Unspecific peroxygenases (UPOs) are promising biocatalysts for oxyfunctionalizations in future sustainable economies and can be efficiently immobilized on the cell surface of their heterologous production yeast. This immobilization has versatile uses, ranging from the mL to m3 scale; but the production of [...] Read more.
Unspecific peroxygenases (UPOs) are promising biocatalysts for oxyfunctionalizations in future sustainable economies and can be efficiently immobilized on the cell surface of their heterologous production yeast. This immobilization has versatile uses, ranging from the mL to m3 scale; but the production of the yeast surface displayed UPOs, and their handling has yet to be optimized to advance sustainable industrial processes in light of the UN’s sustainable development goals. Here, we present optimized production protocols for surface-displayed UPOs for shaken and stirred systems in different scales and describe suitable storage conditions and a sterilization method. We utilized one-factor-at-a-time and design of experiments approaches. We were able to streamline published protocols for shaken flask cultivations to achieve a 60% increase in volumetric activity, using reduced amounts of media. We also show at least a doubling of final activity for bioreactor cultivations by utilizing a different medium than the industry standard. Finally, we present a novel, robust protocol for parallelized methanol-induced enzyme production in Komagataella phaffii in a BioLector XT® reactor. Enzyme activity did not decrease and even increased by our recommended sterilization method and during storage over 87 days. This study aims to advance the yeast surface display immobilization method by providing methods for efficient production, storage and utilization of this promising biocatalyst. Full article
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11 pages, 4560 KiB  
Article
Valorization of Forest Biomass Through Biochar for Static Floating Applications in Agricultural Uses
by Óscar González-Prieto, Luis Ortiz Torres and María Esther Costas Costas
Biomass 2025, 5(3), 44; https://doi.org/10.3390/biomass5030044 - 30 Jul 2025
Viewed by 76
Abstract
The feasibility of utilizing biochar as a static floating material for agricultural applications was researched to prevent evaporation from open water static storage systems or as a floating barrier in slurry pits, for instance. Five types of biochar were created from chips, bark, [...] Read more.
The feasibility of utilizing biochar as a static floating material for agricultural applications was researched to prevent evaporation from open water static storage systems or as a floating barrier in slurry pits, for instance. Five types of biochar were created from chips, bark, and pellets of pine and residues from two acacia species using a pyrolysis time between 60 and 120 min and mean temperatures between 380 and 690 °C in a simple double-chamber reactor. Biomass and biochar were characterized for their main properties: bulk density, moisture content, volatile matter, ash content, fixed carbon, and pH. Biochar was also evaluated through a basic floatability test over 27 days (648 h) in distilled water. The highest fixed carbon content was observed in pine bark biochar (69.5%), followed by the pine pellets (67.4%) and pine chips (63.4%). Despite their high carbon content, the pellets exhibited a low floatability level, whereas pine bark biochar showed superior static floatage times, together with chip and ground chip biochar. These results suggest that biochar produced from bark and wood chips may be suitable for application as floatability material in water or slurry management systems. These results warrant further research into the static floating of biochar. Full article
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