Lignocellulose and starch-based raw materials are often applied in the investigations regarding biohydrogen generation using dark fermentation. Management of the arising post-fermentation broth becomes a problem. The Authors proposed sequential proce...
The aim of the work was to analyze the impact of biochar produced under various production conditions on the course of the dark (hydrogen) fermentation process. A series of experiments were planned, in which the starting material was digestate from a...
Lactic acid fermentation has recently been shown to be a robust storage strategy for food waste prior to conversion to biohydrogen through dark fermentation. However, the importance of initial microbial communities and, more particularly, exogenous m...
The aim of the present paper is the improvement of dark fermentative hydrogen production from problematic substrates. In detail, the study is aimed at (i) investigating the inhibiting effect of two problematic biomasses (i.e., of olive mill wastewate...
The dark fermentation of lignocellulose hydrolysates is a promising process for the production of hydrogen from renewable sources. Nevertheless, hydrogen yields are often lower than those obtained from other carbohydrate sources due to the presence o...
Microbial diversity that thrives in the deep subsurface remains largely unknown. In this work, we present the characterization of Citrobacter sp. T1.2D-1, isolated from a 63.6 m-deep core sample extracted from the deep subsurface of the Iberian Pyrit...
The aim of this review is to provide a comprehensive analysis of the membrane-assisted dark fermentation process for bioH2 production and purification. This review initially analyses the need for and the current state of the art in H2 production thro...
Hydrogen is widely considered as the fuel of the future. Due to the challenges present during hydrogen production using conventional processes and technologies, additional methods must be considered, like the use of microorganisms. One of the most pr...
This study explores biohydrogen production through dark fermentation, an alternative supporting sustainable hydrogen generation. Dark fermentation uses organic waste under anaerobic conditions to produce hydrogen in the absence of light. Key process...
The growing population and economic expansion have led to increased energy demand while presenting complex waste generation and management challenges, particularly in light of climate change. Green hydrogen, which is considered a major clean energy c...
Polyurethane sponge particles were employed as bio-immobilized cell carriers for dark fermentation hydrogen production with heating-treated cow dung inoculum from epipremnum aureum waste and kitchen waste. This study explored the performance changes...
Sorghum distillers grains (SDGs) produced from a sorghum liquor company were used for generating biohydrogen via dark fermentation at pH 4.5–6.5 and 55 °C with a batch test, and the biohydrogen electricity generation potential was evaluated...
One of primary issues in the coffee manufacturing industry is the production of large amounts of undesirable residues, which include the pericarp (outer skin), pulp (outer mesocarp), parchment (endocarp), silver-skin (epidermis) and mucilage (inner m...
Waste-based sustainable solutions proposed by scientific and industrial communities for energy production are an approach that can respond to the growing concerns regarding climate change and fossil resources depletion. This study investigates a two-...
The use of metal nanoparticles (NPs) to enhance hydrogen production in dark fermentation (DF) has become a pioneering field of interest. In particular, iron-based nanoparticles (FeNPs) play a pivotal role in enhancing the activity of metalloenzymes a...
The present study investigates a two-stage process aimed at producing biogas from food waste leachates (FWL) through an experimental approach. The first stage involves biohydrogen production via dark fermentation (DF), while the second focuses on bio...
Hydrogen gas (H2) is an energy carrier that does not generate carbon dioxide emissions during combustion, but several processes in use for its production demand high energy inputs associated with fossil fuels and greenhouse emissions. Biological proc...
The technical feasibility of valorizing tequila vinasse (TV), a wastewater with high pollution potential, through the production of biogenic hydrogen via dark fermentation, has long been proven in diverse lab-scale reactors that were operated either...
The high loading of lactic acid bacteria (LAB) present in cheese whey still limits its use as hydrogen feedstock. This study aims to investigate the feasibility of producing hydrogen from acid cheese whey via lactate-driven dark fermentation (LD-DF)....
Dark fermentation is a hydrogen generating process carried out by anaerobic spore-forming bacteria that metabolize carbon sources producing gas and short-chain acids. The process can be controlled, and the hydrogen harvested if bacteria are grown in...
The pile-fermentation conditions and raw materials used play a vital role in determining the stability and quality of dark tea. In this study, sensory quality evaluation, metabolomics, and microbiomics techniques were used to investigate the effect o...
In recent decades, olive oil consumption has almost tripled worldwide. Olive oil production is linked with the production of enormous amounts of olive mill wastewater, the main by-product derived from three-phase olive mills. Due to the environmental...
Coffee leaves are a promising raw material for functional beverages due to their distinctive phytochemical profile. In this study, sun-dried Arabica coffee leaves were processed into coffee leaf dark tea using Pu-erh-style pile fermentation with thre...
This study aimed at investigating the feasibility of using a highly specialized bacterial inoculum harboring lactic acid bacteria (LAB) and lactate-oxidizing, hydrogen-producing bacteria (LO-HPB) to produce either lactate or biohydrogen and butyrate...
The mechanism of the quality formation of dark tea is not fully clear, particularly under variable fermentation temperatures. In this study, the tea fermented with Aspergillus niger (AN) at 25 (AN25) and 37 °C (AN37) exhibited the highest quality...
In the context of valorizing the organic fraction of urban solid waste (OFMSW) in megacities, dark fermentation emerges as a central strategy alongside composting and anaerobic digestion. This article focuses on assessing the environmental, technical...
Biohydrogen, a low-carbon footprint technology, can play a significant role in decarbonizing the energy system. It uses existing infrastructure, is easily transportable, and produces no greenhouse gas emissions. Four technologies can be used to produ...
The possibility of hydrogen (H2) production from sizing waste, specifically starch-based substrates, was investigated through dark fermentation. Modified starch substrates produced less (up to 54% without heating and 18% after heating) H2 than natura...
Dark fermentation of food waste for biohydrogen production can simultaneously achieve waste resource utilization and clean energy production. However, the widespread application of this technology remains constrained by challenges such as low substra...
Methane and hydrogen generated from waste and biomass are renewable resources, which may successfully replace traditional fossil fuels. This paper investigates the enhancement effect of lignocellulosic biomass pretreatment on dark fermentative hydrog...
In daily life, energy plays a critical role. Hydrogen energy is widely recognized as one of the cleanest energy carriers available today. However, hydrogen must be produced as it does not exist freely in nature. Various methods are available for hydr...
Global energy consumption has been increasing in tandem with economic growth motivating researchers to focus on renewable energy sources. Dark fermentative hydrogen synthesis utilizing various biomass resources is a promising, less costly, and less e...
This study examines the enhancement of dark sequential fermentation and photofermentation of organic solid waste using magnetite and substrate pre-treatment for hydrogen production within the context of transitioning to cleaner energy sources, partic...
The aim of the study was to evaluate the possibility of using the process of dark fermentation to convert kitchen waste into valuable volatile fatty acids in a semi-continuous process at different values of the organic loading rate (2.5 and 5.0 gVS/(...
Despite being recognized as toxic in anaerobic systems, sulfide’s potential to enhance hydrogen fermentation via microbial modulation remains underexplored. This study evaluated the combined effects of sulfide concentration (0–800 mg S/L)...
Mezcal is a drink made in Mexico, the production of which generates vinasses with a high content of organic matter (OM) that is not utilized. However, these residues have the potential to be drawn upon in dark fermentation (DF) processes to obtain bi...
The transition to renewable energy requires scalable and sustainable hydrogen production technologies. Dark fermentation (DF) can generate biohydrogen from diverse biomass feedstock, but its efficiency remains limited. Immobilising anaerobic consorti...
Hydrogen is being increasingly recognized as a promising clean, renewable energy carrier. Among the available production pathways, biological processes, particularly dark fermentation of residual biomasses and agricultural by-products, represent an a...
In this article the concept, the materials and the exploitation potential of a photosynthetic microbial electrochemical cell for the production of hydrogen driven by solar power are investigated. In a photosynthetic microbial electrochemical cell, wh...
Food wastes represent one third of all food produced worldwide. It is crucial to both prevent the production of food waste and recover the wasted fraction with the aim to valorizing it. In this context, the conversion of the organic fraction of munic...
Harnessing hydrogen competently through wastewater treatment using a particular class of biocatalyst is indeed a challenging issue. Therefore, biohydrogen potential of real-field wastewater was evaluated by hybrid fermentative process in a single-st...
In the context of hydrogen production from biomass or organic waste with dark fermentation, this study analysed 55 studies (339 experiments) in the literature looking for the effect of operating parameters on the process performance of dark fermentat...
Here, a comprehensive investigation was conducted under various operational strategies aimed at enhancing biohydrogen production via dark fermentation, with a specific focus on the lactate metabolic pathway, using powdered cheese whey as a substrate....
Used disposable nappies constitute a waste stream that has no established treatment method. The purpose of this study was the assessment of the dark fermentation of used disposable nappies and expired food products under different pH values. The biod...
This study presents the analysis and estimation of the hydrogen production from coffee mucilage mixed with organic wastes by dark anaerobic fermentation in a co-digestion system using an artificial neural network and fuzzy logic model. Different rati...
The aim of this study was to determine the optimal conditions for dark fermentation using agro-industrial liquid wastewaters mixed with sweet sorghum stalks (i.e., 55% sorghum, 40% cheese whey, and 5% liquid cow manure). Batch experiments were perfor...
A major limitation to the conversion of OFMSW via anaerobic fermentation is the high concentration of animal-derived wastes, which can inhibit the process due to ammonia accumulation. This study assessed the reusability of ammonia-loaded, dark fermen...
Integrated dark fermentation (DF), anaerobic digestion (AD), and biogas reforming have been proposed for converting food waste to hydrogen and are usually assessed by yield. This study proves that total hydrogen is fixed by the chemical oxygen demand...
Background: Dark fermentation (DF) of organic solid waste (OSW) is a promising route for sustainable biohydrogen (bioH2) production, but it is often limited by slow interspecies electron transfer and by acidification of the medium. Promoting direct i...
Adequate treatment of the organic fraction of municipal solid waste (OFMSW) in co-digestion with sewage sludge (SS) through dark fermentation (DF) technologies has been widely studied and recognized. However, there is little experience with a high-so...
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