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Microorganisms
Special Issues
Microbial Bioprocesses
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Microbial Bioprocesses

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Microbial Biotechnology".

Deadline for manuscript submissions: 30 November 2025 | Viewed by 2232

Special Issue Editor

Dr. Walter José Martínez-Burgos

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Guest Editor
Department of Bioprocess Engineering and Biotechnology, Polytechnic Center, Federal University of Parana, Rua Cel. Francisco H. dos Santos—100, Curitiba 81530-000, PR, Brazil
Interests: industrial processes; bioprocesses; biotechnology; bioenergy; fermentative processes
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The Special Issue entitled "Microbial Bioprocesses" highlights advances and innovations in the use of microorganisms for biotechnological processes in different areas, such as health, environment, food and energy. With a focus on sustainable processes and the application of emerging technologies, this collection brings together studies on the production of biopharmaceuticals, biopolymers, biofuels, as well as strategies for bioremediation and bioconversion. In addition, it addresses the development of new bioprocesses, genetic engineering of microorganisms and the optimization of fermentation conditions, seeking greater efficiency, cost reduction and minimal environmental impact. The Issue is an important reference for researchers, academics and professionals interested in exploring the potential of microorganisms to solve global challenges and promote the bioeconomy.

Dr. Walter José Martínez-Burgos
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microbial biotechnology
  • fermentation
  • biopharmaceuticals
  • biofuels
  • biopolymers
  • bioremediation
  • bioconversion
  • metabolic engineering
  • industrial microorganisms
  • sustainable production
  • bioeconomy
  • bioprocess optimization
  • emerging technologies
  • sustainability

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Published Papers (2 papers)

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Research

20 pages, 1912 KiB  
Article
Actinobacillus succinogenes in Bioelectrochemical Systems: Influence of Electric Potentials and Carbon Fabric Electrodes on Fermentation Performance
by Julian Tix, Jan-Niklas Hengsbach, Joshua Bode, Fernando Pedraza, Julia Willer, Sei Jin Park, Kenneth F. Reardon, Roland Ulber and Nils Tippkötter
Microorganisms 2025, 13(8), 1720; https://doi.org/10.3390/microorganisms13081720 - 23 Jul 2025
Viewed by 301
Abstract
The fermentation of Actinobacillus succinogenes in bioelectrochemical systems offers a promising approach to enhance biotechnological succinate production by shifting the redox balance towards succinate and simultaneously enabling CO2 utilization. Key process parameters include the applied electric potential, electrode material, and reactor design. [...] Read more.
The fermentation of Actinobacillus succinogenes in bioelectrochemical systems offers a promising approach to enhance biotechnological succinate production by shifting the redox balance towards succinate and simultaneously enabling CO2 utilization. Key process parameters include the applied electric potential, electrode material, and reactor design. This study investigates the influence of various carbon fabric electrodes and applied potentials on product distribution during fermentation of A. succinogenes. Building on prior findings that potentials between −600 mV and –800 mV increase succinate production, recent data reveal that more negative potentials, beyond the water electrolysis threshold, trigger electrochemical side reactions, altering product yields. Specifically, succinate decreased from 19.76 ± 0.41 g∙L−1 to 14.1 ± 1.6 g∙L−1, while lactate rose from 0.59 ± 0.12 g∙L−1 to 3.12 ± 0.21 g∙L−1. Contrary to common assumptions, the shift is not primarily driven by oxygen formation. Instead, the results indicate that the intracellular redox potential is affected by both the applied potential and hydrogen evolution, which alters metabolic pathways to maintain redox balance. These findings demonstrate that more negative applied potentials in electro-fermentation processes can impair succinate yields, emphasizing the importance of fine-tuning electrochemical conditions in the system for optimized biotechnological succinate production. Full article
(This article belongs to the Special Issue Microbial Bioprocesses)
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18 pages, 2919 KiB  
Article
De Novo Assembly of the Polyhydroxybutyrate (PHB) Producer Azohydromonas lata Strain H1 Genome and Genomic Analysis of PHB Production Machinery
by Daniele Traversa, Carlo Pazzani, Pietro D’Addabbo, Lucia Trisolini, Matteo Chiara, Marta Oliva, Angelo Marzella, Camilla Mandorino, Carla Calia, Guglielmina Chimienti, Caterina Manzari, Graziano Pesole and Maria Scrascia
Microorganisms 2025, 13(1), 137; https://doi.org/10.3390/microorganisms13010137 - 10 Jan 2025
Viewed by 1314
Abstract
Polyhydroxybutyrate (PHB) is a biodegradable natural polymer produced by different prokaryotes as a valuable carbon and energy storage compound. Its biosynthesis pathway requires the sole expression of the phaCAB operon, although auxiliary genes play a role in controlling polymer accumulation, degradation, granule formation [...] Read more.
Polyhydroxybutyrate (PHB) is a biodegradable natural polymer produced by different prokaryotes as a valuable carbon and energy storage compound. Its biosynthesis pathway requires the sole expression of the phaCAB operon, although auxiliary genes play a role in controlling polymer accumulation, degradation, granule formation and stabilization. Due to its biodegradability, PHB is currently regarded as a promising alternative to synthetic plastics for industrial/biotechnological applications. Azohydromonas lata strain H1 has been reported to accumulate PHB by using simple, inexpensive carbon sources. Here, we present the first de novo genome assembly of the A. lata strain H1. The genome assembly is over 7.7 Mb in size, including a circular megaplasmid of approximately 456 Kbp. In addition to the phaCAB operon, single genes ascribable to PhaC and PhaA functions and auxiliary genes were also detected. A comparative genomic analysis of the available genomes of the genus Azohydromonas revealed the presence of phaCAB and auxiliary genes in all Azohydromonas species investigated, suggesting that the PHB production is a common feature of the genus. Based on sequence identity, we also suggest A. australica as the closest species to which the phaCAB operon of the strain H1, reported in 1998, is similar. Full article
(This article belongs to the Special Issue Microbial Bioprocesses)
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