Search Results (26,042)

The inclusion of the Internet of Things (IoT) in indoor agricultural systems has become a fundamental tool for improving cultivation systems by providing key information for decision-making in pursuit of better performance. This article presents the design and implementation of an IoT-based agricultural system installed in a plant growth chamber for hydroponic cultivation under controlled conditions. The growth chamber is equipped with sensors for air temperature, relative humidity (RH), carbon dioxide (CO₂) and photosynthetically active photon flux, as well as control mechanisms such as humidifiers, full-spectrum Light Emitting Diode (LED) lamps, mini split air conditioner, pumps, a Wi-Fi surveillance camera, remote monitoring via a web application and three Nutrient Film Technique (NFT) hydroponic systems with a capacity of ten plants each. An ATmega2560 microcontroller manages the smart system using the MODBUS RS-485 communication protocol. To validate the proper functionality of the proposed system, a case study was conducted using lettuce crops, in which the impact of different nutrient solution concentrations (50%, 75% and 100%) on the phenotypic development and nutritional content of the plants was evaluated. The results obtained from the cultivation experiment, analyzed through analysis of variance (ANOVA), show that the treatment with 75% nutrient concentration provides an appropriate balance between resource use and nutritional quality, without affecting the chlorophyll content. This system represents a scalable and replicable alternative for protected agriculture. Full article

Hazelnut is an important crop worldwide, and the characteristics of the fruits are quite variable according to a number of factors, including variety and cultivation conditions, which in turn can vary according to harvest year. This study aimed to investigate some physical and chemical characteristics of three hazelnut varieties grown in Portugal (Grada de Viseu, Tonda di Giffoni and Butler) along two different harvesting years (2021 and 2022). Also, the microbial quality was investigated for its relevance to the conservation of the fruits. The physical properties evaluated were biometric characteristics and colour, the chemical components analysed were moisture, lipids, protein, ash and fibre, and the microbial properties investigated were the microorganisms, moulds and yeasts. The results showed that, generically, statistically significant differences were found between the three varieties under study on several properties investigated. The kernel was confirmed as the lighter part of all hazelnuts, and when comparing between varieties, Tonda di Giffoni presented the lighter fruits in both harvesting years. With respect to weight, the Tonda di Giffoni variety was the lightest in both harvest years. Moisture content was observed to be higher than the recommended limits for two of the samples (Grada de Viseu in 2021: 6.01 ± 0.26 g/100 g and Butler in 2022: 6.02 ± 0.37 g/100 g), although the difference was marginal given that the recommended limit is 6%. Not surprisingly, lipids were the major chemical component, ranging from 66.46 ± 1.67 to 70.14 ± 1.75 g/100 g in 2021 and from 64.38 ± 1.67 to 77.77 g/100 g in 2022. It was further observed that the three varieties presented a satisfactory microbiological quality. Finally, applying factor analysis with principal components and Varimax rotation, a solution that explains 92.8% of the variance was obtained. This study provided information that is relevant for the characterisation and evaluation of variability according to the year of hazelnuts of three varieties cultivated in Portugal. Full article

Anthracnose, a significant disease of aloe with characteristics of contact transmission, poses a considerable threat to the economic viability of aloe cultivation. To address the challenges of accurately detecting and classifying crop diseases in complex environments, this study proposes an enhanced algorithm, YOLOv11-seg-DEDB, based on the improved YOLOv11-seg model. This approach integrates multi-scale feature enhancement and a dynamic attention mechanism, aiming to achieve precise segmentation of aloe anthracnose lesions and effective disease level discrimination in complex scenarios. Specifically, a novel Disease Enhance attention mechanism is introduced, combining spatial attention and max pooling to improve the accuracy of lesion segmentation. Additionally, the DCNv2 is incorporated into the network neck to enhance the model’s ability to extract multi-scale features from targets in challenging environments. Furthermore, the Bidirectional Feature Pyramid Network structure, which includes an additional p2 detection head, replaces the original PANet network. A more lightweight detection head structure is designed, utilizing grouped convolutions and structural simplifications to reduce both the parameter count and computational load, thereby enhancing the model’s inference capability, particularly for small lesions. Experiments were conducted using a self-collected dataset of aloe anthracnose infected leaves. The results demonstrate that, compared to the original model, the improved YOLOv11-seg-DEDB model improves segmentation accuracy and mAP@50 for infected lesions by 5.3% and 3.4%, respectively. Moreover, the model size is reduced from 6.0 MB to 4.6 MB, and the number of parameters is decreased by 27.9%. YOLOv11-seg-DEDB outperforms other mainstream segmentation models, providing a more accurate solution for aloe disease segmentation and grading, thereby offering farmers and professionals more reliable disease detection outcomes. Full article

Soybean (Glycine max (L.) Merrill) is a major oilseed crop rich in phytosterols and tocopherols, compounds associated with functional and nutritional properties of vegetable oils. This study aimed to apply, for the first time, Independent Component Analysis (ICA) to discriminate the composition of phytosterols (β-sitosterol, campesterol, stigmasterol) and tocopherols (α, β, γ, δ) in 20 soybean genotypes—14 non-transgenic and six transgenic—cultivated in two major producing regions of Paraná state, Brazil (Londrina and Ponta Grossa). Lipophilic compounds were extracted from soybean seeds, quantified via gas chromatography and HPLC, and statistically analyzed using ICA with the JADE algorithm. The extracted independent components successfully differentiated soybean varieties based on phytochemical profiles. Notably, transgenic cultivars from Ponta Grossa exhibited higher levels of total tocopherols, including α- and β-tocopherol, while conventional cultivars from both regions showed elevated phytosterol content, particularly campesterol and stigmasterol. ICA proved to be a powerful unsupervised method for visualizing patterns in complex compositional data. These findings highlight the significant influence of genotype and growing region on the nutraceutical potential of soybean, and support the use of multivariate analysis as a strategic tool for cultivar selection aimed at enhancing functional quality in food applications. Full article

Widespread antibiotic residues are accumulating in the environment, potentially causing adverse effects for humans, animals, and the ecosystem, including an increase in antibiotic-resistant bacteria, resulting in worldwide concern. There are various commonly used physical, chemical, and biological treatments for the degradation of antibiotics. However, the elimination of toxic end products generated by physicochemical methods and the need for industrial applications pose significant challenges. Hence, environmentally sustainable, green, and readily available approaches for the transformation and degradation of these antibiotic compounds are being sought. Herein, we review the impact of sustainable fungal laccase-based bioremediation strategies. Fungal laccase enzyme is considered one of the most active enzymes for biotransformation and biodegradation of antibiotic residue in vitro. For industrial applications, the low laccase yields in natural and genetically modified hosts may constitute a bottleneck. Methods to screen for high-laccase-producing sources, optimizing cultivation conditions, and identifying key genes and metabolites involved in extracellular laccase activity are reviewed. These include advanced transcriptomics, proteomics, and metagenomics technologies, as well as diverse laccase-immobilization technologies with different inert carrier/support materials improving enzyme performance whilst shifting from experimental assays to in situ monitoring of residual toxicity. Still, more basic and applied research on laccase-mediated bioremediation of pharmaceuticals, especially antibiotics that are recalcitrant and prevalent, is needed. Full article

Petroglyph motifs from 23 sites and 37 panels in northern Georgia and western North Carolina foothills and mountains are analyzed within their archaeological, ethnographic, and landscape contexts. The Track Rock Tradition comprises 10 chronologically sequenced marking categories: (1) Cupules/Meanders/Open Circles; (2) Soapstone Extraction cars; (3) Vulva Shapes; (4) Figures; (5) Feet/Hands/Tracks; (6) Nested Circles; (7) Cross-in-Circles; (8) Spirals; (9) Straight Lines; and (10) Thin Incised Lines. Dating spans approximately 3800 years. Early cupules and meanders predate 3000 years ago, truncated by Late Archaic soapstone extraction. Woodland period (3000–1050 years ago) motifs include vulva shapes, figures, feet, tracks, and hands. Early Mississippian concentric circles date to 1050–600 years ago, while Middle Mississippian cross-in-circles span 600–350 years ago. Late Mississippian spirals (350–200 years ago) and post-contact metal tool incisions represent the most recent phases. The Track Rock Tradition differs from western Trapp and eastern Hagood Mill traditions. Given the spatial overlap with Iroquoian-speaking Cherokee territory, motifs are interpreted through Cherokee beliefs, supplemented by related Muskogean Creek ethnography. In Cherokee cosmology, the matrilocal Thunderers hierarchy includes the Female Sun/Male Moon, Selu (Corn Mother)/Kanati (Lucky Hunter), Medicine Woman/Judaculla (Master of Game), and Little People families. Ritual practitioners served as intermediaries between physical and spirit realms through purification, fasting, body scratching, and rock pecking. Meanders represent trails, rivers, and lightning. Cupules and lines emphasize the turtle appearance of certain rocks. Vulva shapes relate to fertility, while tracks connect to life-giving abilities. Concentric circles denote townhouses; cross-in-circles and spirals represent central fires. The tradition shows continuity in core beliefs despite shifting emphases from hunting (Woodland) to corn cultivation (Mississippian), with petroglyphs serving as necessary waypoints for spiritual supplicants. Full article

Euterpe precatoria Mart. is an increasingly important palm for subsistence and income generation in central and western Amazonia with growing demand for its fruit pulp, which is an alternative source of açaí juice for domestic and international markets. This study synthesizes current knowledge on its systematics, ecology, fruit production in natural populations, fruit quality, uses, population management, and related areas, identifying critical research gaps. A systematic literature survey was conducted across databases including Web of Science, Scopus, Scielo, CAPES, and Embrapa. Of 1568 studies referencing Euterpe, 273 focused on E. precatoria, with 90 addressing priority themes. Genetic diversity studies suggest the E. precatoria may represent a complex of species. Its population abundance varies across habitats: the highest variability occurs in terra firme, followed by baixios and várzeas. Várzeas exhibit greater productivity potential, with more bunches per plant and higher fruit weight than baixios; no production data exist for terra firme. Additionally, E. precatoria has higher anthocyanin content than E. oleracea, the primary commercial açaí species. Management of natural populations and cultivation practices are essential for sustainable production; however, studies in these fields are still limited. The information is crucial to inform strategies aiming to promote the sustainable production of the species. Full article

Grapevine (Vitis vinifera L.) cultivation is an important agricultural sector worldwide. Its expansion into new areas, like Kazakhstan, brings significant phytosanitary risks. Viroids, such as grapevine yellow speckle viroid 1 (GYSVd-1) and hop stunt viroid (HSVd), are RNA pathogens that threaten vineyard productivity. They can cause a progressive decline through latent infections. Traditional diagnostic methods are usually targeted and therefore not suitable for thorough surveillance. In contrast, modern high-throughput sequencing (HTS) methods often face challenges due to their high costs and complicated sample preparation, such as ribosomal RNA (rRNA) depletion. This study introduces a simplified diagnostic workflow that overcomes these barriers. We utilized the latest Oxford Nanopore V14 cDNA chemistry, which is designed to prevent internal priming, by substituting a targeted oligo(dT)VN priming strategy to facilitate the sequencing of non-polyadenylated viroids from total RNA extracts, completely bypassing the rRNA depletion step and use of random oligonucleotides for c DNA synthesis. This method effectively detects and identifies both GYSVd-1 and HSVd. This workflow significantly reduces the time, cost, and complexity of HTS-based diagnostics. It provides a powerful and scalable tool for establishing strong genomic surveillance and phytosanitary certification programs, which are essential for supporting the growing viticulture industry in Kazakhstan. Full article

Peanut cultivation is widely practiced using plastic mulch film, resulting in the accumulation of microplastics/nanoplastics (MPs/NPs) in agricultural soils, potentially negatively affecting peanut growth. To investigate the effects of two polystyrene (PS) sizes (5 μm, 50 nm) and three concentrations (0, 10, and 100 mg L⁻¹) on peanut growth, photosynthetic efficiency, and physiological characteristics, a 15-day hydroponic experiment was conducted using peanut seedlings as the experimental material. The results indicated that PS-MPs/NPs inhibited peanut growth, reduced soil and plant analyzer development (SPAD) values (6.7%), and increased levels of malondialdehyde (MDA, 22.0%), superoxide anion (O₂⁻, 3.8%) superoxide dismutase (SOD, 16.1%) and catalase (CAT, 12.1%) activity, and ascorbic acid (ASA, 12.6%) and glutathione (GSH, 9.1%) contents compared to the control. Moreover, high concentrations (100 mg L⁻¹) of PS-MPs/NPs reduced the peanut shoot fresh weight (16.1%) and SPAD value (7.2%) and increased levels of MDA (17.1%), O₂⁻ (5.6%), SOD (10.6%), POD (27.2%), CAT (7.3%), ASA (12.3%), and GSH (6.8%) compared to low concentrations (10 mg L⁻¹) of PS-MPs/NPs. Notably, under the same concentration, the impact of 50 nm PS-NPs was stronger than that of 5 μm PS-MPs. The peanut shoot fresh weight of PS-NPs was lower than that of PS-MPs by an average of 7.9%. Additionally, we found that with an increasing exposure time of PS-MPs/NPs, the inhibitory effect of low concentrations of PS-MPs/NPs on the fresh weight was decreased by 2.5%/9.9% (5 d) and then increased by 7.7%/2.7% (15 d). Conversely, high concentrations of PS-MPs/NPs consistently reduced the fresh weight. Correlation analysis revealed a clear positive correlation between peanut biomass and both the SPAD values as well as Fv/Fm, and a negative correlation with MDA, SOD, CAT, ASA, and GSH. Furthermore, the presence of PS-MPs/NPs in roots, stems, and leaves was confirmed using a confocal laser scanning microscope. The internalization of PS-MPs/NPs within peanut tissues negatively impacted peanut growth by increasing the MDA and O₂⁻ levels, reducing the SPAD values, and inhibiting the photosynthetic capacity. In conclusion, the study demonstrated that the effects of PS on peanuts were correlated with the PS size, concentration, and exposure time, highlighting the potential risk of 50 nm to 5 μm PS being absorbed by peanuts. Full article

Adult trees of pedunculate oak (Quercus robur L.) are recalcitrant to vegetative propagation. In this study, we investigated the micropropagation of five oak genotypes corresponding to trees aged 60–800 years in a liquid medium. We used commercial RITA bioreactors to study the influence of the explant type, the culture medium, shoot support and number of immersions. Variables evaluated included the number of normal and hyperhydric shoots, shoot length, multiplication coefficient and number of rootable shoots per explant. All genotypes could be cultured in temporary immersion. Basal stem sections attached to callus grew better than apical sections and developed less hyperhydricity. For long-term cultivation, Gresshoff and Doy medium was the best of the three media evaluated. All genotypes produced vigorous shoots suitable for rooting and acclimation. This is the first protocol to proliferate adult oak trees in bioreactors, representing significant progress towards large-scale propagation of this and other related species. Full article

Sustainability education has become a global priority in educational systems, aiming to equip learners with the knowledge, values, and skills necessary to address complex environmental and social challenges. This study specifically aims to understand the role of science education in promoting students’ awareness of sustainability and their understanding of the interconnected relationship between humans and the environment, based on the perceptions and practices of primary science teachers in Al-Ahsa, Saudi Arabia. A qualitative approach was utilized, which included semi-structured interviews complemented by classroom observations as primary data collection instruments. The targeted participants comprised a purposive sample consisting of forty-nine primary-level science instructors from the Al-Ahsa district, located in eastern Saudi Arabia. Emergent concepts from open and axial coding processes by using grounded theory were developed with the gathered data. Based on the findings, teachers perceive science teaching not only as knowledge delivery but as an opportunity to cultivate critical thinking and nurture eco-friendly actions among pupils. Classroom practices that underscore environmental values and principles of sustainability foster a transformative view of the teacher’s role beyond traditional boundaries. The data also highlighted classroom practices that integrate environmental values and sustainability principles, reflecting a transformative perspective on the teacher’s educational role. Full article

This study evaluated biomass accumulation and phenolic compound production in seven Hypericum species (H. androsaemum, H. calycinum, H. hirsutum, H. kalmianum, H. olympicum, H. perforatum, and H. triquetrifolium) cultivated in vitro under varying growth regulator treatments and culture periods. Shoots were grown on Murashige and Skoog (MS) medium supplemented with benzyladenine (BA) or meta-topoline (mT) and analyzed after 40 and 60 days. MS medium supplemented with 0.2 mg/L BA was the most effective condition for promoting biomass across all species, with shoot fresh weight increasing significantly at 60 days, particularly in H. olympicum, H. perforatum, and H. triquetrifolium. High-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS) identified 13 phenolic compounds, including flavonols, hydroxycinnamic acids, anthocyanins, phloroglucinols, and naphthodianthrones. Phenolic profiles were species-specific and influenced by culture period. H. kalmianum accumulated the highest total phenolic content (37.6 mg/g DW), while H. olympicum was the top producer of hypericin and pseudohypericin. These results highlight the crucial role of culture conditions in regulating both biomass and phytochemical production and provide a promising approach for producing bioactive metabolites in Hypericum species through in vitro systems. Full article

Gastrodia elata Blume is an important medicinal orchid, yet its large-scale cultivation is increasingly threatened by fungal diseases. The 4-coumarate–CoA ligase (4CL) gene family directs a key step in phenylpropanoid metabolism and plant defence, but its composition and function in G. elata have not been investigated. We mined the G. elata genome for 4CL homologues, mapped their chromosomal locations, and analysed their gene structures, conserved motifs, phylogenetic relationships, promoter cis-elements and codon usage bias. Publicly available transcriptomes were used to examine tissue-specific expression and responses to fungal infection. Subcellular localisation of selected proteins was verified by transient expression in Arabidopsis protoplasts. Fourteen Ge4CL genes were identified and grouped into three clades. Two members, Ge4CL2 and Ge4CL5, were strongly upregulated in tubers challenged with fungal pathogens. Ge4CL2 localised to the nucleus, whereas Ge4CL5 localised to both the nucleus and the cytoplasm. Codon usage analysis suggested that Escherichia coli and Oryza sativa are suitable heterologous hosts for Ge4CL expression. This study provides the first genome-wide catalogue of 4CL genes in G. elata and suggests that Ge4CL2 and Ge4CL5 may participate in antifungal defence, although functional confirmation is still required. The dataset furnishes a foundation for functional characterisation and the molecular breeding of disease-resistant G. elata cultivars. Full article

In river-connected lake regions, both land use and hydrological regime changes may affect the ecosystem services; however, few studies have attempted to elucidate their complex influences. In this study, the spatiotemporal dynamics of eight ecosystem services (crop production, aquatic production, water yield, soil retention, flood regulation, water purification, net primary productivity, and habitat quality) were investigated through remote-sensing images and the InVEST model in the Dongting Lake Region during 2000–2020. Results revealed that crop and aquatic production increased significantly from 2000 to 2020, particularly in the northwestern and central regions, while soil retention and net primary productivity also improved. However, flood regulation, water purification, and habitat quality decreased, with the fastest decline in habitat quality occurring at the periphery of the Dongting Lake. Land-use types accounted for 63.3%, 53.8%, and 40.3% of spatial heterogeneity in habitat quality, flood regulation, and water purification, respectively. Land-use changes, particularly the expansion of construction land and the conversion of water bodies to cropland, led to a sharp decline in soil retention, flood regulation, water purification, net primary productivity, and habitat quality. In addition, crop production and aquatic production were higher in cultivated land and residential land, while the accompanying degradation of flood regulation, water purification, and habitat quality formed a “production-pollution-degradation” spatial coupling pattern. Furthermore, hydrological fluctuations further complicated these dynamics; wet years amplified agricultural outputs but intensified ecological degradation through spatial spillover effects. These findings underscore the need for integrated land-use and hydrological management strategies that balance human livelihoods with ecosystem resilience. Full article

Amidst global climate change, carbon emissions across the edible vegetable oil supply chain are critical for sustainable development. This paper systematically reviews the existing literature, employing life cycle assessment (LCA) to analyze key factors influencing carbon footprints at stages including cultivation, processing, and transportation. It reveals the differential impacts of fertilizer application, energy structures, and regional policies. Unlike previous reviews that focus on single crops or regions, this study uniquely integrates global data across major edible oils, identifying three critical gaps: methodological inconsistency (60% of studies deviate from the requirements and guidelines for LCA); data imbalance (80% concentrated on soybean/rapeseed); weak policy-technical linkage. Key findings: fertilizer emissions dominate cultivation (40–60% of total footprint), while renewable energy substitution in processing reduces emissions by 35%. Future efforts should prioritize multidisciplinary integration, enhanced data infrastructure, and policy scenario analysis to provide scientific insights for the low-carbon transformation of the global edible oil industry. Full article