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14 pages, 1033 KB  
Article
The Effect of Organic Production on the Sugar and Organic Acid Concentration in Different Sour Cherry Cultivars
by Alicja Ponder, Renata Kazimierczak, Małgorzata Żebrowska-Krasuska, Dominika Średnicka-Tober, Agnieszka Głowacka and Ewelina Hallmann
Appl. Sci. 2026, 16(2), 1092; https://doi.org/10.3390/app16021092 - 21 Jan 2026
Viewed by 77
Abstract
Sour cherry is one of the most popular stone fruits in Poland. In the organic production system of sour cherries, no artificial pesticides and fertilizers are allowed, which is one of the organic production requirements increasingly appreciated by producers and consumers. The taste [...] Read more.
Sour cherry is one of the most popular stone fruits in Poland. In the organic production system of sour cherries, no artificial pesticides and fertilizers are allowed, which is one of the organic production requirements increasingly appreciated by producers and consumers. The taste of fruits is created by the sugar and organic acid content and their ratio. Vitamin C is known for its health-promoting properties. The aim of the present study was to analyze and compare the concentrations of vitamin C, sugars, and organic acids and their profiles in organic vs. conventional sour cherry fruits representing different cultivars, in a three-year experiment. In the presented experiment, four sour cherry cultivars, ‘Kelleris’ 16, ‘Oblacińska’, ‘Pandy 103’, and ‘Debreceni Bötermö’, were cultivated in two horticultural systems, organic and conventional, and the content of sugars and organic acids was analyzed in the fruit with HPLC methods. Organically cultivated sour cherry fruits were characterized by significantly higher concentrations of sugars and vitamin C only in the first year of the experiment, when the mean concentrations of fructose, glucose, and sucrose in these fruits reached 4.15 g/100 g F.W., 0.37 g/100 g F.W., and 0.27 g/100 g F.W., respectively, and the concentration of vitamin C reached 17.28 mg/100 g F.W. In the two subsequent years, conventional cherries were more abundant in these compounds. Among the tested sour cherry cultivars, ‘Oblačińska’ performed the best in terms of sugar content. The mean value for total sugars for ‘Oblačińska’ cv. was 5.53 g/100 g F.W. In the case of vitamin C, the highest levels (av. 28.13 mg/100 g F.W.) were noted in the fruits of ‘Pandy 103’ cv. The strong year-to-year variability underscores the need for multi-year experiments and, where possible, multi-site trials, to disentangle cultivar × system × environment interactions. Because the quality of sour cherry for fresh consumption and for processing depends mostly on sugar content, for organic production, ‘Oblačińska’ cv. is strongly recommended. Full article
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29 pages, 2094 KB  
Article
Phytomedicines for Mental Disorders in Hungary—Questionnaire and Phytochemical Analysis of Herbal OTC Products
by Tibor Rák, Edit Ormai and Györgyi Horváth
Sci. Pharm. 2026, 94(1), 10; https://doi.org/10.3390/scipharm94010010 - 15 Jan 2026
Viewed by 148
Abstract
Mental health disorders, particularly anxiety and insomnia, are increasingly prevalent worldwide, prompting interest in herbal-based complementary therapies. This study surveyed 168 Hungarian healthcare professionals to evaluate their knowledge and recommendations regarding herbal sedatives and analyzed seven commonly suggested OTC products available in Hungary, [...] Read more.
Mental health disorders, particularly anxiety and insomnia, are increasingly prevalent worldwide, prompting interest in herbal-based complementary therapies. This study surveyed 168 Hungarian healthcare professionals to evaluate their knowledge and recommendations regarding herbal sedatives and analyzed seven commonly suggested OTC products available in Hungary, using thin-layer chromatography (TLC) and UV–Vis spectrophotometry according to the European Pharmacopoeia. The survey revealed that 86.9% of respondents recommend herbal products for nervous system complaints, with Valeriana officinalis and Melissa officinalis being the preferred ingredients. Herbal teas and traditional herbal medicines were the most frequently suggested product categories. Laboratory analysis confirmed the presence of marker compounds in all tested products; however, significant variability in active ingredient concentrations was observed. One homeopathic product contained an unidentified alkaloid-like compound, raising safety concerns. Essential oil yields from tea mixtures also varied markedly, and some products did not meet pharmacopoeial standards for hypericin content. These findings highlight the popularity of phytotherapy among healthcare professionals and the need for stricter quality control of OTC herbal sedatives. Future research should include multi-batch analyses and clinical trials to establish robust evidence for efficacy and safety. Full article
(This article belongs to the Topic Natural Products and Drug Discovery—2nd Edition)
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20 pages, 730 KB  
Article
Fortification of Durum Wheat Pasta with Mealworm (Tenebrio molitor) Powder: Physicochemical, Nutraceutical, and Sensory Effects
by Ewelina Zielińska, Paulina Sidor and Urszula Pankiewicz
Molecules 2026, 31(2), 298; https://doi.org/10.3390/molecules31020298 - 14 Jan 2026
Viewed by 166
Abstract
Edible insects are gaining popularity as an alternative food source, highlighting the urgent need for research on their incorporation into traditional food products. This study investigated the impact of incorporating mealworm (Tenebrio molitor) powder (MP) at 2%, 5%, and 10% levels [...] Read more.
Edible insects are gaining popularity as an alternative food source, highlighting the urgent need for research on their incorporation into traditional food products. This study investigated the impact of incorporating mealworm (Tenebrio molitor) powder (MP) at 2%, 5%, and 10% levels on the nutritional, functional, and sensory properties of pasta. Proximate composition, mineral content, color parameters, cooking quality, antioxidant activity and sensory properties were evaluated. Starch digestibility fractions and predicted glycemic index (pGI) were calculated based on in vitro enzymatic hydrolysis. Results showed that 10% MP addition significantly increased protein (1.45-fold) and fat content (12-fold), enriched minerals (Fe, Zn, Mg, K), and improved antioxidant capacity (ABTS+·: 1.3-fold; DPPH·: 2.6-fold) and phenolic content (14.4-fold) compared to control. Color analysis revealed a decrease in lightness and an increase in redness, indicating darker tones with higher MP levels. This supplementation reduced rapidly digestible starch and pGI while increasing slowly digestible starch, suggesting benefits for glycemic control. Sensory evaluation revealed no significant differences (p > 0.05) among samples for appearance, color, taste, and overall impression, confirming good acceptability. Overall, MP fortification improved nutritional and functional properties without compromising sensory quality, supporting its application in developing high-protein, health-oriented foods. Full article
(This article belongs to the Special Issue Functional Foods Enriched with Natural Bioactive Compounds)
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26 pages, 1279 KB  
Systematic Review
The Impact of Game-Based Learning on Motivation, Self-Efficacy, and Academic Achievement in the Natural Sciences: A Meta-Analysis
by José Gabriel Soriano-Sánchez, Rocío Quijano López and Diego Airado Rodríguez
Educ. Sci. 2026, 16(1), 122; https://doi.org/10.3390/educsci16010122 - 14 Jan 2026
Viewed by 446
Abstract
Game-based learning has become an increasingly popular educational methodology due to its ability to enhance student interest and engagement. The aim of this study was to analyze the effect of game-based learning on motivation, self-efficacy, and academic performance in Natural Sciences learning. A [...] Read more.
Game-based learning has become an increasingly popular educational methodology due to its ability to enhance student interest and engagement. The aim of this study was to analyze the effect of game-based learning on motivation, self-efficacy, and academic performance in Natural Sciences learning. A systematic review and meta-analytic methodology was employed, following PRISMA guidelines. For this purpose, the databases consulted were Web of Science and Scopus, from which a total of 234 documents were retrieved and reduced to 15 studies after rigorously applying the established eligibility criteria. These studies were included in the systematic review and meta-analysis to ensure the validity and relevance of the meta-analytic findings. The meta-analytic results revealed a very strong and highly significant positive effect across all subgroups, benefiting the experimental groups (Z = 6.29; p < 0.00001). In conclusion, the implementation of game-based learning has a positive impact on motivation, self-efficacy, and academic performance in the teaching and learning of Natural Sciences content. Therefore, its incorporation into pedagogical practices represents an opportunity to strengthen student engagement and promote more meaningful learning. Full article
(This article belongs to the Section STEM Education)
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14 pages, 2245 KB  
Article
Study on the Tensile Properties and Influencing Factors of Superelastic SMAF-Reinforced PP/PVA-ECC Materials
by Yan Cao, Xiaolong Qi and Zhao Yang
Materials 2026, 19(2), 263; https://doi.org/10.3390/ma19020263 - 8 Jan 2026
Viewed by 192
Abstract
To develop a cost-effective shape memory alloy fiber-reinforced engineered cementitious composite (SMAF-ECC) with excellent mechanical properties, polypropylene (PP) fibers were used to partially replace polyvinyl alcohol (PVA) fibers to prepare the ECC matrix, and superelastic shape memory alloy fibers (SMAFs) were incorporated to [...] Read more.
To develop a cost-effective shape memory alloy fiber-reinforced engineered cementitious composite (SMAF-ECC) with excellent mechanical properties, polypropylene (PP) fibers were used to partially replace polyvinyl alcohol (PVA) fibers to prepare the ECC matrix, and superelastic shape memory alloy fibers (SMAFs) were incorporated to fabricate a novel SMAF-ECC. Uniaxial tensile tests were systematically performed to characterize the tensile mechanical properties of the composites, focusing on the effects of SMAF volume content and diameter. The results indicate that the optimal base ECC mix proportion is 0.8 vol.% PP fibers and 1.2 vol.% PVA fibers, achieving an ultimate tensile strain of 4.88% (only a 4.69% reduction compared to pure PVA-ECC) while significantly reducing material cost without sacrificing superior ductility. SMAF volume content and diameter notably influence the tensile performance of SMAF-ECC, with the specimen containing 0.2 mm diameter SMAFs at 0.2 vol.% exhibiting the best performance: initial cracking stress, ultimate tensile stress, and ultimate tensile strain are enhanced by 16.79%, 20.85%, and 2.87%, respectively, compared to pure ECC. This study provides a theoretical basis and parametric guidance for the engineering popularization and application of cost-effective SMAF-ECCs. Full article
(This article belongs to the Section Construction and Building Materials)
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13 pages, 288 KB  
Article
Yield, Nutritional Quality, and Microbial Safety of Microgreens Grown in Insect Frass and Vermicompost-Based Growing Substrates
by Giuseppe Di Cuia, Massimiliano D’Imperio, Federico Baruzzi, Alessia Marzulli, Francesco Gai and Angelo Parente
Agronomy 2026, 16(2), 158; https://doi.org/10.3390/agronomy16020158 - 8 Jan 2026
Viewed by 417
Abstract
Microgreens have gained increasing popularity due to their cooking versatility, ease of cultivation, and high nutritional value. The use of alternative organic substrates, such as vermicompost and insect frass, offers a promising alternative to peat. This study has evaluated the integration of Tenebrio [...] Read more.
Microgreens have gained increasing popularity due to their cooking versatility, ease of cultivation, and high nutritional value. The use of alternative organic substrates, such as vermicompost and insect frass, offers a promising alternative to peat. This study has evaluated the integration of Tenebrio molitor and Hermetia illucens frass, along with vermicompost, in a microgreen production, while assaying several concentrations (25%, 50%, 75%, and 100%) as replacements by weight. After a preliminary assay aimed at determining the optimal frass and vermicompost levels, we assessed the agronomic, nutritional, and microbiological performances of microgreens. The preliminary results revealed phytotoxic effects of T. molitor frass, while the addition of H. illucens frass or vermicompost did not significantly impact microgreen production. In the second experiment, the interaction between plant species and substrate composition significantly influenced the leaf area, plant height, and mineral content. Partial replacement of peat with H. illucens frass or vermicompost enhanced leaf area and plant height, with a notable increase in iron content in the mizuna microgreens grown with H. illucens frass, compared to the control with peat. Additionally, microbiological safety was ensured, and a complete absence of Salmonella spp. and E. coli was observed in the plants, in accordance with European food safety regulations. Full article
(This article belongs to the Section Horticultural and Floricultural Crops)
30 pages, 10996 KB  
Article
Visitor Satisfaction at the Macau Science Center and Its Influencing Factors Based on Multi-Source Social Media Data
by Jingwei Liang, Qingnian Deng, Yufei Zhu, Jiahai Liang, Chunhong Wu, Liang Zheng and Yile Chen
Information 2026, 17(1), 57; https://doi.org/10.3390/info17010057 - 8 Jan 2026
Viewed by 435
Abstract
With the rise in experience economy and the popularization of digital technology, user-generated content (UGC) has become a core data source for understanding tourist needs and evaluating the service quality of venues. As a landmark venue that combines science education, interactive experience, and [...] Read more.
With the rise in experience economy and the popularization of digital technology, user-generated content (UGC) has become a core data source for understanding tourist needs and evaluating the service quality of venues. As a landmark venue that combines science education, interactive experience, and landscape viewing, the service quality of the Macau Science Center directly affects tourists’ travel experience and word-of-mouth dissemination. However, existing studies mostly rely on traditional questionnaire surveys and lack multi-technology collaborative analysis. In order to accurately identify the factors affecting satisfaction, this study uses 788 valid UGC data from five major platforms, namely Google Maps reviews, TripAdvisor, Sina Weibo, Xiaohongshu (Rednote), and Ctrip, from January 2023 to November 2025. It integrates word frequency analysis, semantic network analysis, latent Dirichlet allocation (LDA) topic modeling, and Valence Aware Dictionary and sEntiment Reasoner (VADER) sentiment computing to construct a systematic research framework. The study found that (1) the core attention dimensions of users cover the needs of parent–child and family visits, exhibitions and interactive experiences, ticketing and consumption services, surrounding environment and landscape, emotional evaluation, and recommendation intention. (2) The keyword association network has gradually developed from a loose network in the early stage to a comprehensive experience-dense network. (3) LDA analysis identified five main potential demand themes: comprehensive visiting experience and scenario integration, parent–child interaction and characteristic scenario experience, core venue facilities and ticketing services, visiting value and emotional evaluation, and transportation and surrounding landscapes. (4) User emotions were predominantly positive, accounting for 82.7%, while negative emotions were concentrated in local service details, and the emotional scores showed a fluctuating upward trend. This study provides targeted suggestions for the service optimization of the Macau Science Center and also provides a methodological reference for UGC-driven research in similar cultural venues. Full article
(This article belongs to the Special Issue Social Media Mining: Algorithms, Insights, and Applications)
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16 pages, 235 KB  
Entry
Popular Culture in a Digital Society: Nine Paradoxes
by Sue Spaid
Encyclopedia 2026, 6(1), 12; https://doi.org/10.3390/encyclopedia6010012 - 6 Jan 2026
Viewed by 439
Definition
This entry, which identifies nine paradoxes particular to popular culture in a digital society, begins by distinguishing art and culture, since scholars have historically relied on these terms to differentiate popular culture, mass culture, and mass art. Digital societies, which exist both online [...] Read more.
This entry, which identifies nine paradoxes particular to popular culture in a digital society, begins by distinguishing art and culture, since scholars have historically relied on these terms to differentiate popular culture, mass culture, and mass art. Digital societies, which exist both online and offline, are awash in digital products such as LED signs, digital imagery, video games, film, podcasts, and social media. In a digital society, popular culture is effectively “mass art,” which exhibits five properties: (1) digital media’s low-cost products and low-skill tools are (2) created and distributed to appeal to as broad a cultural sector as possible (qualitative) and thus aim to (3) attract consumers (quantitative) who capably enjoy and deploy cultural content both (4) offline and online, yet “popularity” ultimately depends on (5) efforts to maximize unity and minimize fragmentation. Except for localized events, popular culture has largely disappeared, while mass art will likely flourish until human beings clamor once again for firsthand experiences or go extinct. The next frontier will be finding ways to prevent artificial intelligence from producing cultural products, not because they will be terrible, undesirable, or fake, but because the culture-making process itself engenders human wellbeing. Full article
(This article belongs to the Section Social Sciences)
31 pages, 1852 KB  
Article
Sentiment Analysis of X Users Regarding Bandung Regency Using Support Vector Machine
by Irlandia Ginanjar, Abdan Mulkan Shabir, Anindya Apriliyanti Pravitasari, Sinta Septi Pangastuti, Gumgum Darmawan and Sukono
Appl. Sci. 2026, 16(1), 560; https://doi.org/10.3390/app16010560 - 5 Jan 2026
Viewed by 203
Abstract
Social media has the potential to serve beneficial purposes. The abundance of uploaded content and responses from the public generates various opinions, allowing them to be identified as positive or negative regarding the portrayal of Bandung Regency. This research aims to analyse the [...] Read more.
Social media has the potential to serve beneficial purposes. The abundance of uploaded content and responses from the public generates various opinions, allowing them to be identified as positive or negative regarding the portrayal of Bandung Regency. This research aims to analyse the classification and frequency of words for each sentiment expressed by X (Twitter) users regarding Bandung Regency. The research employs the Support Vector Machine (SVM) method. We expect the results to aid in formulating governmental programmes for Bandung Regency. The research revealed that the SVM model, which uses the Sigmoid kernel function with parameters C = 10 and gamma (γ) = 1, is the most optimal sentiment classification model for handling an imbalanced dataset. This model achieved an 83.01% negative recall value. Furthermore, frequent words appearing in both classes indicate that several positive opinions about Bandung Regency exhibit similar dominance, except for football dominance in negative opinions. This research pertains to the United Nations Sustainable Development Goals (SDGs), particularly SDG 11 (Sustainable Cities and Communities) and SDG 16 (Peace, Justice, and Strong Institutions). The suggested technique facilitates evidence-based policy reviews, transparent governance, and enhanced responsive public services by analysing public sentiment regarding local government performance. The results illustrate how social media analytics can aid local governments in assessing popular sentiment and pinpointing areas for policy response. Full article
(This article belongs to the Special Issue Natural Language Processing and Text Mining)
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26 pages, 8912 KB  
Article
A Novel Leuconostoc mesenteroides Direct-Vat-Set Starter with High Nitrite-Degrading Activity for Safe and High-Quality Fermentation of Chinese Dongbei Suancai
by Xiaoou Zhao, Ruochen Huang, Luobing Zhao, Lei Wang, Yunhui Zhao, Xin Zhang, Xiangshu Jin, Duojia Wang and Xiaoxiao Liu
Fermentation 2026, 12(1), 30; https://doi.org/10.3390/fermentation12010030 - 5 Jan 2026
Viewed by 359
Abstract
Dongbei suancai is a popular traditional fermented vegetable in Northeast China. However, the conventional production methods often lead to nitrite accumulation and quality fluctuations, which to some extent constrain the development towards health and scale. To address this, a novel Direct-Vat-Set (DVS) starter [...] Read more.
Dongbei suancai is a popular traditional fermented vegetable in Northeast China. However, the conventional production methods often lead to nitrite accumulation and quality fluctuations, which to some extent constrain the development towards health and scale. To address this, a novel Direct-Vat-Set (DVS) starter was developed based on L. mesenteroides AA001, a strain isolated from traditional fermented foods and possessing high nitrite-degrading capability. By optimizing the culture medium and cryoprotectant formulation, the cell density and freeze-drying survival rate were significantly enhanced. Crucially, the freeze-drying process did not impair the core degradation function of the strain, with the nitrite degradation rate remaining above 90%. The DVS starter was applied to Dongbei suancai fermentation, effectively inhibiting nitrite accumulation while simultaneously increasing organic acid content and optimizing the proportion of essential amino acids. It simultaneously suppressed the growth of undesirable bacteria and, under low-temperature conditions, steered the microbial community toward metabolic activities, ensuring the controllability and safety of the fermentation process. Therefore, inoculation with the L. mesenteroides AA001 DVS starter effectively inhibits nitrite accumulation, enabling a highly efficient, stable, and clean fermentation process that significantly improves the quality of Dongbei suancai. Full article
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15 pages, 2274 KB  
Article
Coupled Effects of Tree Species and Understory Morel on Modulating Soil Microbial Communities and Nutrient Dynamics
by Xia Yuan, Haiyan Qin, Yun Wang, Shuwen Wu, Zeyu Zhang, Muxin Fan, Li Li, Liuqian Tian and Yiwen Fu
Microorganisms 2026, 14(1), 99; https://doi.org/10.3390/microorganisms14010099 - 2 Jan 2026
Viewed by 296
Abstract
Morel mushrooms (Morchella spp.) are highly prized for their culinary and economic value. Understory cultivation, leveraging the symbiotic relationship between morels and trees, has gained increasing popularity. However, the effects of this practice on belowground microbial communities and nutrient dynamics remain poorly [...] Read more.
Morel mushrooms (Morchella spp.) are highly prized for their culinary and economic value. Understory cultivation, leveraging the symbiotic relationship between morels and trees, has gained increasing popularity. However, the effects of this practice on belowground microbial communities and nutrient dynamics remain poorly understood. In this study, we examined how understory cultivation of morels (Morchella sextelata) under five different tree species affects soil bacterial and fungal communities, as well as nutrient availability and mineral element content. The results revealed that soil physicochemical properties responded variably to morel cultivation under different tree species. Notably, understory morel cultivation reduced soil NO3-N by 38–67% across tree species, whereas NH4+-N remained stable, reflecting the distinct nutrient preference of Morchella and associated trees, and suggesting targeted nitrate fertilization could mitigate nitrogen limitations. Understory cultivation significantly increased soil mineral elements, with Zelkova serrata (Z. serrata) showing the highest concentrations, elevating available potassium (AK), calcium (ECa), manganese (AMn) and boron (AB) by approximately 20%, 13%, 30%, and 168%, highlighting its potential for soil quality improvement. Microbial community composition was also significantly altered, with fungal communities exhibiting more pronounced shifts than bacterial communities, likely due to their closer ecological associations with morels. Importantly, Z. serrata markedly promoted microbial-mediated soil carbon and nitrogen accumulation, driven by mineral binding, root secretions and soil pH value. These findings enhance understanding of belowground effects of morel understory cultivation, revealing that select tree species like Z. serrata can improve soil quality and nutrient cycling, while targeted nitrate fertilization supports sustaining morel cultivation systems. Full article
(This article belongs to the Special Issue State-of-the-Art Environmental Microbiology in China 2025)
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16 pages, 2337 KB  
Article
Autofluorescence Profiling of Virgin Olive Oil: Impact of Rosemary and Basil Flavoring During Storage
by Enrique J. Díaz-Montaña, Ramón Aparicio-Ruiz, Noelia Tena, Ana Lobo-Prieto, Diego L. García-González and María Teresa Morales
Antioxidants 2026, 15(1), 62; https://doi.org/10.3390/antiox15010062 - 1 Jan 2026
Viewed by 406
Abstract
The consumption of virgin olive oil has been associated with a broad spectrum of beneficial effects. These health outcomes are attributed not only to its high monounsaturated fatty acid content but also to its bioactive components. Nowadays, the flavoring of olive oil has [...] Read more.
The consumption of virgin olive oil has been associated with a broad spectrum of beneficial effects. These health outcomes are attributed not only to its high monounsaturated fatty acid content but also to its bioactive components. Nowadays, the flavoring of olive oil has gained popularity to improve its antioxidant properties, modify its sensory characteristics, and enhance its oxidative stability. This study explores spectrofluorometry as a fast, non-destructive, and eco-friendly tool to monitor oxidation and predict shelf life in virgin olive oils (VOOs). Both unflavored and flavored rosemary and basil samples were studied. Over nine months of storage, monthly autofluorescence measurements at 330 nm excitation revealed dynamic spectral changes. These changes were mapped into three distinct emission zones (I, II, and III), providing a spectral fingerprint of oil freshness and stability. Autofluorescence analysis revealed that oxidation-related emission increased while pigment-related emission decreased over time, especially within the first five months. Rosemary and basil flavoring slowed degradation due to antioxidant migration from the herbs. It is proposed that a ratio between the fluorescence intensity of Zone III/Zone II of the spectrum of less than 0.6 indicates oils stored for more than three months. Full article
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27 pages, 1936 KB  
Review
Bioactive Chemicals and Biological Activity of Tropaeolum majus L. and the Importance of Trichoderma spp. in the Cultivation of This Species
by Sylwia Skazińska, Roman Andrzejak, Katarzyna Waszkowiak and Beata Janowska
Agriculture 2026, 16(1), 101; https://doi.org/10.3390/agriculture16010101 - 31 Dec 2025
Viewed by 291
Abstract
Tropaeolum majus L. is a popular ornamental plant. All parts of T. majus plant (flowers, leaves, and seeds) are edible and are appreciated for their pungent taste, although their chemical composition varies. T. majus is known for its many health benefits. It is [...] Read more.
Tropaeolum majus L. is a popular ornamental plant. All parts of T. majus plant (flowers, leaves, and seeds) are edible and are appreciated for their pungent taste, although their chemical composition varies. T. majus is known for its many health benefits. It is a source of trace elements and bioactive compounds that are easily absorbed by the human body. The flowers of T. majus contain flavonoids from the flavone and flavonol groups, as well as their glycosides, which exhibit antibacterial, antifungal and antiviral activity. They also inhibit the activity of certain enzymes. Among the flavonoids, the flowers and leaves of T. majus contain derivatives of kaempferol and quercetin. Flavonoids also include anthocyanins, which are responsible for the color of T. majus flowers. In red flowers, delphinidin predominates; in orange flowers, pelargonidin; and in yellow flowers, pelargonidin and delphinidin are present in similar amounts. In the flowers of T. majus, seven carotenoids have been identified: violaxanthin, antheraxanthin, lutein, zeaxanthin, α, β and γ-carotene. In the leaves, however, lutein, violaxanthin, β-carotene and neoxanthin were detected. In T. majus, the presence of two glucosinolates has been reported: glucotropaeolin and sinalbin. The flowers and leaves of T. majus also contain both macroelements (N, P, K, Ca, Mg, Na) and microelements (Fe, Mn, Cu, Zn, Mo), and essential oils which have anti-cancer, antibacterial, and antiviral properties. The quality and flowering of T. majus are enhanced by fungi of the Trichoderma genus, which is important both ecologically and in terms of increasing the yield of raw material extracted from the plant. T. aureoviride, T. hamatum, and T. harzianum stimulated the flowering of the T. majus ‘Spitfire’. The plants treated with T. harzianum after being planted in pots flowered the most abundantly. Trichoderma spp. caused the plants to grow more intensively, producing longer and more leafy shoots with a greater number of offshoots. Trichoderma spp. stimulated the uptake of macronutrients, except for P. In the case of Ca and Na, this phenomenon was only observed in plants treated with T. aureoviride and T. hamatum, and for Mg, only when T. hamatum was applied to sown seeds. As for the developed root systems, as far as the micronutrients are concerned, Trichoderma spp. stimulated the uptake of Zn and Mn. Additionally, there was a higher Fe content in the plants treated with T. harzianum on both dates and T. aureoviride after planting the plants in pots. Full article
(This article belongs to the Special Issue The Application of Trichoderma in Crop Production)
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23 pages, 6878 KB  
Article
Phenotype, Squalene, and Lanosterol Content Variation Patterns During Seed Maturation in Different Leaf-Color Tea Cultivars
by Jing-Jing Ye, Yu-Ning Fang, Xiao-Quan Lu, Shu-Ling Dong, Yue-Rong Liang, Jian-Liang Lu, Kai-Rong Wang, Long-Jie Zhang and Xin-Qiang Zheng
Foods 2026, 15(1), 94; https://doi.org/10.3390/foods15010094 - 29 Dec 2025
Viewed by 318
Abstract
Squalene and lanosterol are bioactive compounds with diverse physiological effects, found in relatively high concentrations in tea seed oil. Their levels are significantly influenced by cultivar and fruit maturity. As leaf-color specific tea cultivars gain popularity, parts of them tend to have a [...] Read more.
Squalene and lanosterol are bioactive compounds with diverse physiological effects, found in relatively high concentrations in tea seed oil. Their levels are significantly influenced by cultivar and fruit maturity. As leaf-color specific tea cultivars gain popularity, parts of them tend to have a higher flower and fruit ratio than green-leaf tea cultivars. However, their fruit characteristics remain underexplored. This study investigated 15 tea cultivars with different leaf colors, analyzing phenotypic changes in seeds during maturation, and examining the variation patterns of squalene and lanosterol. The crude water content, dry kernel content, and oil content were closely related to the maturity and effectively reflected seed development. Lanosterol content showed an overall downward trend with increased maturity. Squalene content fluctuated sharply before the seeds fully matured, but gradually decreased once they were fully matured. At full maturity, leaf-color specific tea cultivars generally exhibited higher concentrations of squalene and lanosterol than those with green leaf. Full article
(This article belongs to the Section Plant Foods)
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22 pages, 2367 KB  
Article
Harnessing the Potential of a Secondary Metabolite-Based Formulation for the Post-Harvest Disease Management and Shelf Life Extension of Banana
by Karma Beer, T. Damodaran, M. Muthukumar, Prasenjit Debnath, Akath Singh and Maneesh Mishra
Metabolites 2026, 16(1), 22; https://doi.org/10.3390/metabo16010022 - 25 Dec 2025
Viewed by 369
Abstract
Background: Post-harvest losses in bananas, particularly due to diseases such as anthracnose and stem-end rot, significantly limit their storage life and marketability. Developing effective and non-toxic treatments to prolong the shelf life of fruits while maintaining quality is crucial inenabling long-distance transport and [...] Read more.
Background: Post-harvest losses in bananas, particularly due to diseases such as anthracnose and stem-end rot, significantly limit their storage life and marketability. Developing effective and non-toxic treatments to prolong the shelf life of fruits while maintaining quality is crucial inenabling long-distance transport and facilitating exports. Methods: The most popular and commercial banana variety, ‘Grand Naine’, was treated with a proprietary secondary metabolite-based formulation (this refers to a solution containing natural compounds produced by living organisms, which are not directly involved in growth but can influence various biological processes, such as antimicrobial activity) and stored under cold conditions at 13 °C, using vacuum packaging (a method where air is removed from the packaging to reduce spoilage and prolong freshness). Untreated fruits were considered as controls, meaning that they were not subjected to the treatment and served as a baseline for comparison. Shelf life-related parameters such as ethylene production (a plant hormone responsible for triggering fruit ripening), ACC oxidase activity (an enzyme central to ethylene synthesis), respiration rate (the rate at which fruit consumes oxygen and produces carbon dioxide), firmness, total soluble solids (TSS; measures the sugar content in fruit), acidity, and metabolic composition were assessed, including indices of susceptibility to disease. These measurements were taken at regular intervals for both treated and control fruits. Results: Secondary metabolite-treated bananas maintained quality for 45 days, staying free from anthracnose and stem-end rot. Control fruits showed over-ripening and an 11.6% percent disease index (PDI). Treated fruits had lower ethylene production (7.80 μg/kg/s vs. 10.03 μg/kg/s in controls), reduced ACC oxidase activity, and a slower respiration rate, delaying ripening. They also had greater firmness (1.45 kg/cm2), optimal TSS (13.5 °Brix), balanced acidity (0.58%), and increased flavonoid and antioxidant levels compared to controls. Conclusions: Secondary metabolite-based treatment, combined with cold storage and vacuum packaging, extended banana shelf life to 45 days, minimized disease, and preserved fruit quality. This approach substantially reduced post-harvest losses, demonstrating export potential through extended storage. Full article
(This article belongs to the Section Food Metabolomics)
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