Search Results (458)

The Latvian Dark-head (Latvijas tumšgalve; LT) is the only sheep breed developed in Latvia. It is fully adapted to the country’s climatic conditions. As the sole national breed, it holds notable cultural importance by supporting traditional husbandry practices, landscape preservation, and regional identity. However, the dominance of commercial breeds threatens local genetic resources. Small-scale farms, where the LT population is concentrated, are especially vulnerable to population decline and possible extinction. This study assesses the genetic diversity within the LT breed and compares it with that of other major sheep breeds in Latvia. For the first time, lambs from sire rams of major breeds in Latvia underwent genotyping using the Illumina Ovine SNP50 BeadChip^® (San Diego, CA, USA). Genetic diversity was assessed with minor allele frequency (MAF) analysis. Breed-specific markers were identified by detecting fixed SNPs (MAF = 0) unique to each breed. In total, 27,561 highly polymorphic SNPs (MAF 0.3–0.5) were identified in the LT breed. This indicates substantial genetic differentiation from other sheep breeds raised in Latvia. Among the analyzed SNPs, 2668 (5.45%) were fixed in the LT breed. Of these, 55–131 were unique compared with other breeds. The OvineSNP50 panel is an effective tool for characterizing the genetic structure of the LT breed. It enables the detection of distinct genetic traits and interbreed differences. These results establish a genomic basis for targeted conservation and selective breeding strategies. Such strategies maintain the genetic integrity and competitiveness of the Latvian Dark-head. Full article

The concept of bioregions stands out among the various approaches to sustainable territorial development, as it prioritises the initiative of local communities in addressing sustainability issues. At the same time, the concept of bioregions is diverse in practice, focusing on organic farming, tourism, local culture and also nature protection. Therefore, a balanced approach to the development of bioregions, which considers the specific characteristics of a particular area and the needs of its local community, is important. The present research employed the Analytical Hierarchy Process (AHP) approach to analyse economic, ecological and social value added as the main criteria, alongside their subordinate subcriteria. As a result, three scenarios were designed: the Broad Integration Scenario, the Conservative Scenario and the Culture and Tourism Scenario. The dominant strategy was found to be the Culture and Tourism scenario, focusing on tourism and local cultural values. This strategy can contribute to increasing the consumption of local products and services while preserving the cultural values and ensuring a balanced approach to sustainable development. At the same time, the Broad Integration Scenario revealed that a broad—and perhaps an aggressive—approach to sustainable development was highly controversial, as it had the greatest dispersion in priority vector values. Bioregionalism, however, is a little-supported approach that does not gain support from either sustainability or rapid growth advocates. The results reveal the importance of a long-term approach to sustainable development and, at the same time, implicitly point to the role of public attitudes, both in terms of supply (through local community initiatives in bioregionalisation) and demand, which could increase the consumption of bioregional products and services. Full article

Pulpitis is the inflammatory response of the dental pulp to microbial challenge and can range from mild to severe in nature, with severe pulpitis traditionally resulting in pulp removal and root canal treatment (RCT). In the pursuit of more conservative treatments, recent clinical practice guidelines have recommended strategies that preserve the vitality of the dental pulp, rather than RCT, when possible. This has increased the focus on improving the accuracy of pulp diagnosis, which will direct treatment and improve management outcomes. Unfortunately, current point-of-care (PoC) tools are subjective, lack discrimination and rely on the stimulation of pulpal neurons, limiting dentists’ ability to objectively identify the level of inflammation. Molecular biomarker assessment has the potential to dynamically analyse pulpitis and correlate this with inflammatory thresholds and treatment outcomes. Numerous chemokines, cytokines, proteases and growth factors exhibit altered expression during pulpitis and can be collected intraoperatively as part of routine dental treatment. Although current data indicate several markers that could be used as next-generation diagnostic chairside tools for pulpitis, there are currently no commercial kits. Considering the interest in vital pulp treatment, there is an urgent need to engage researchers, industry, dentists and other stakeholders in the development of PoC diagnostic assays for pulpitis. Full article

Domestic donkeys (Equus africanus asinus) are an important livestock genetic resource that is currently considered at risk. The number of donkey breeds worldwide is declining due to their loss of function as working animals in rural communities. Local breeds with small populations, which are threatened by genetic erosion and are at risk of extinction, require urgent action to characterize and preserve their genetic diversity. As microarrays containing thousands of single-nucleotide polymorphisms (SNPs) are not yet available for these species, traditional molecular markers such as microsatellites and mitochondrial DNA (mtDNA) remain valuable tools for genetic monitoring and management. This study uses different molecular markers to assess and manage genetic variability in endangered Sicilian donkey breeds. This information can support breeding plans and mating schemes, as well as in situ and ex situ conservation programs. The practical application of molecular information in the conservation strategies for these breeds is briefly discussed, and the proposed approach is considered transferable to other threatened breeds. Full article

The critical need to preserve cultural relics has sustained longstanding interest in their corrosion and conservation, research vital to extending artifact lifespan and maintaining historical authenticity. Although scholarly output in this field grows annually, the expanding volume of literature makes it difficult to systematically identify research hotspots and forecast trends. This lack of clarity can lead to redundant efforts and hinder the practical application of preservation technologies. Existing reviews often focus on specialized subtopics, leaving a comprehensive overview lacking. To address this gap, this study conducts a systematic bibliometric analysis of 4983 relevant publications from the WOS Core Collection (1961–2025). Through a multi-dimensional examination of annual publication trends, keyword co-occurrence, contributions from countries and authors, and institutional collaborations, we elucidate the field’s development and intellectual structure. Our findings reveal key research hotspots, including corrosion mechanisms, novel protective materials, micro-environmental control, and multidisciplinary detection methods, whose evolution shows distinct temporal patterns. Furthermore, an analysis of collaborative networks indicates that progress is increasingly driven by institutional and international cooperation, steering the field toward greater systematization and refinement. Full article

The digitization of historic architecture increasingly relies on dense point clouds, yet the conversion of these datasets into structured Historic Building Information Models (HBIM) remains slow, inconsistent, and heavily dependent on manual interpretation. This paper introduces a classification-driven, mesh-based semi-automated workflow designed to close this gap by providing a controlled, repeatable path from raw TLS data to BIM-ready geometry. The method combines three elements strategically integrated into a unified framework: (1) pre-classified point cloud groups that establish a structured starting point, (2) mesh simplification and slice-based geometric reconstruction executed through Rhino and Grasshopper, and (3) direct BIM integration using Rhino.Inside.Revit to generate categorized HBIM components rather than passive mesh imports. The workflow is validated on an irregular exterior stone column from the historic chapel in Sopronhorpács, Hungary, an element characterized by surface erosion, asymmetric profiles, and deviations from verticality. This type of geometry typically challenges both manual modeling and fully automated shape-fitting. The proposed method reconstructed the column as a Revit Structural Column element with a substantial reduction in modeling time compared to a manual Scan-to-BIM workflow. A deviations analysis confirmed that the reconstructed geometry remained within the millimeter-level accuracy required for conservation-grade documentation. The study demonstrates that combining element-based classification, mesh preprocessing, and controlled semi-automation can significantly improve both the speed and reliability of Scan-to-HBIM processes without requiring technical expertise yet delivers results that align with the precision expected in scientific documentation. By formalizing the Pre-Classified Modeling Logic (PCML), the approach provides a foundation for reconstructing a wide range of heritage elements and establishes a practical step forward toward more efficient, interpretable, and accessible digital preservation practices. Full article

This study investigates the relationship between architectural colour and cultural identity in the ancient villages of Hebei Province, emphasising the role of colour in cultural heritage preservation and sustainable development. The research aims to (1) identify the dominant chromatic attributes of Hebei’s village architecture, (2) interpret their cultural and symbolic meanings, and (3) construct a colour model applicable to heritage conservation. A qualitative case study approach was employed across four representative villages, combining field surveys, semi-structured interviews, and digital colour analysis using the COLORO system. The findings reveal that the prevailing hues—ranging from red and yellow to ochre and brown—derive from local stone and timber, embodying values of stability, humility, and harmony with the environment. Decorative elements in bright red and gold signify celebration and community vitality, contrasting with the subdued architectural tones. Integrating these empirical and cultural insights, this study proposes the Colour Symbol System for Hebei Ancient Villages (CSSHAV)—a model that unites quantitative colour parameters with qualitative cultural interpretation. The CSSHAV serves as a practical framework for guiding colour conservation, policy development, and sustainable design in rural heritage contexts. The originality of this study lies in bridging scientific colour measurement with cultural semiotics, providing both theoretical advancement and actionable guidance for the preservation of regional chromatic identity. The findings identify increasing risks of colour homogenisation under the pressures of globalisation. Through the CSSHAV model, it proposes strategies to preserve Hebei’s traditional chromatic identity by integrating digital colour analysis with cultural interpretation. This balance between conservation and innovation contributes to sustaining both the aesthetic integrity and cultural vitality of ancient villages. Full article

Ethiopic manuscript studies have become a rapidly expanding field in recent decades. However, most research has focused on cataloging and textual analysis. This study examines the material traces of original addenda, patterns of deterioration, and desecration of indigenous conservation ethics. A combination of codicological and paleographic methods was used. This approach is vital for documenting historical features, understanding the context of use, and informing conservation efforts. The research involved assessing twenty-eight physical manuscripts from two collections in Addis Ababa, Ethiopia. Additionally, twenty-seven digital copies from the Endangered Archives Program, the Hill Museum & Manuscript Library, and the University of Cambridge Digital Library repositories were consulted. The findings revealed original features like holes, repairs, and scribal corrections. Damage such as tears, creases, dirt, fading, erasures, and recent writing was also identified. These results reveal the material history of the manuscripts. Furthermore, both domestic and international stakeholders have adversely affected these manuscripts through erasure, dispossession, and appropriation. This study proposes ethical guidelines for recent additions to the manuscripts and for preserving the original addendum. It also underscores the necessity for additional material research, enhancements in conservation practices, and efforts to raise awareness. Full article

Based on existing models, this paper incorporates some key ecological factors, thereby obtaining a class of eco-epidemiological models that can more objectively reflect natural phenomena. This model simultaneously integrates disease dynamics within the prey population and the Beddington–DeAngelis functional response, thus achieving an organic combination of ecological dynamics, epidemic transmission, and spatial movement under time-varying environmental conditions. The proposed framework significantly enhances ecological realism by simultaneously accounting for spatial dispersal, predator–prey interactions, disease transmission within prey species, and seasonal or temporal variations, providing a comprehensive mathematical tool for analyzing complex eco-epidemiological systems. The theoretical results obtained from this study can be summarized as follows: Firstly, the existence and uniqueness of globally positive solutions for any positive initial data are rigorously established, ensuring the well-posedness and biological feasibility of the model over extended temporal scales. Secondly, analytically tractable sufficient conditions for uniform population persistence are derived, which elucidate the mechanisms of species coexistence and biodiversity preservation even under sustained epidemiological pressure. Thirdly, by employing innovative applications of differential inequalities and fixed point theory, the existence and uniqueness of a positive spatially homogeneous periodic solution in the presence of time-periodic coefficients are conclusively demonstrated, capturing essential rhythmicities inherent in natural systems. Fourthly, through a sophisticated combination of the upper and lower solution method for parabolic partial differential equations and Lyapunov stability theory, the global asymptotic stability of this periodic solution is rigorously established, offering a powerful analytical guarantee for long-term predictive modeling. Beyond theoretical contributions, these research findings provide actionable insights and quantitative analytical tools to tackle pressing ecological and public health challenges. They facilitate the prediction of thresholds for maintaining ecosystem stability using real-world data, enable the analysis and assessment of disease persistence in spatially structured environments, and offer robust theoretical support for the planning and design of wildlife management and conservation strategies. The derived criteria support evidence-based decision-making in areas such as controlling zoonotic disease outbreaks, maintaining ecosystem stability, and mitigating anthropogenic impacts on ecological communities. A representative numerical case study has been integrated into the analysis to verify all of the theoretical findings. In doing so, it effectively highlights the model’s substantial theoretical value in informing policy-making and advancing sustainable ecosystem management practices. Full article

The focus of this paper is on the large-format wooden panel painting Maundy Thursday Altarpiece from Southern Germany. Its wooden support and paint layer were severely damaged due to high climatic fluctuations, above all dryness. The aim of the research project was to develop a low-risk, conservatively acceptable procedure for controlled in situ humidification. In an interdisciplinary approach, a practical monitoring concept on-site was linked to art technology analyses, surface monitoring, hygrothermal simulations, and climate chamber tests. Based on the results, an individual climate corridor for controlled humidification of the case study was developed with the help of an enclosure and implemented in two gradual moistening phases. The combination of conservative support, measurement technology, and digital assessment allowed a controlled approach to a conservation optimum without other active interventions in the original material. The results highlight the need for object-specific strategies and humidity corridors at the interface between conservation, climate adaptation, and sustainability. A deviation from museum standard recommendations (depending on the guidelines 40–60% rH) shows the special challenges of monument preservation. Full article

Wooden cultural relics, as significant components of historical and cultural heritage, are persistently threatened by deterioration due to biological, environmental, and chemical factors. Addressing these issues is crucial not only for the conservation of cultural heritage but also for ensuring its sustainable transmission to future generations. This review systematically examines international research on the degradation and preservation of wooden cultural relics, outlining the temporal evolution, key contributing nations and research groups, major thematic focuses, and their interrelationships. We comprehensively summarize the mechanisms underlying wood degradation—including microbial attack, chemical degradation, and stress-induced deformation—and evaluate advanced techniques for detecting and assessing deterioration. Furthermore, we analyze the critical environmental and material variables that influence degradation rates. Building on this foundation, the paper surveys current mainstream conservation methodologies, such as physical and chemical reinforcement, desiccation, and drying treatments. Special emphasis is placed on emerging strategies that leverage novel materials and technologies, for instance, biomimetic hydrophobic coatings to prevent liquid water penetration and nanomaterial-based approaches for multifunctional surface treatment. Finally, we discuss persistent challenges and prospective research directions in the field, aiming to inform future scientific studies and advance practical conservation efforts for wooden cultural heritage. Full article

This study applies a quantitative model to assess natural resources and degradation risks in mountain ecosystems, focusing on ecotourism’s role in balancing economic growth and environmental preservation in Golija Nature Park. Results show moderate tourism potential and low degradation risk, affirming ecotourism’s feasibility under sustainable management. The integration of natural assets, cultural heritage, and rural communities highlights ecotourism’s capacity to strengthen local economies, support demographic revitalization, and enhance biodiversity conservation in mountainous regions. The proposed approach offers a practical model for sustainable planning and management of natural areas in a broader international context. Full article

Pangolins (Manidae), recognized for their distinctive keratinous scales, are the world’s most trafficked mammals. This is primarily due to their extensive use in traditional medicine systems in Africa and Asia, where their body parts, particularly their scales, are used in various health practices. This high demand fueled widespread poaching and illegal wildlife trade, placing immense pressure on their populations. A critical knowledge gap persists regarding the specific body parts utilized and the global extent of pangolin use in traditional medicine, which hinders effective conservation strategies and demand mitigation efforts. Bridging the divide between traditional knowledge and modern scientific research is essential to ensure both cultural practices and species preservation. Therefore, this review comprehensively examines the complex relationship between pangolins and human health practices. It explores the cultural, medicinal, and ecological significance of pangolins, addresses sustainability challenges of their populations, and outlines the key drivers of their exploitation. Full article

Background: Lipedema is a progressive adipofascial disorder marked by painful nodular fat deposition that is often mistaken for obesity. While tumescent liposuction reduces limb volume with relative lymphatic safety, persistent large, painful lobules frequently remain, and excisional strategies risk iatrogenic lymphatic injury. We evaluated the application of intraoperative indocyanine green (ICG) lymphography to identify and preserve lymphatic channels during debulking surgery for symptomatic lipedema. Methods: We conducted a single-center case series (University of Pittsburgh Medical Center, July 2023–December 2024) of adults with lipedema refractory to conservative therapy who underwent a selective dermato-lipectomy (lobule/skin excision) with or without tumescent liposuction. Patients with clinical lymphedema or dermal backflow in ICG were excluded. Near-infrared ICG (SPY-PHI) was used for pre-incision mapping and real-time intraoperative guidance; lymphatic trajectories were marked and spared during lobule excision. Primary measures included dermal backflow patterns and lymph node transit time; secondary outcomes were complications and symptom burden (Lymphedema Life Impact Scale, LLIS) through ≥24 months. Results: Eight patients (five female/three male; mean age 49.5 ± 14.4 years; median BMI 52.65 kg/m²) underwent ICG-guided surgery. Preoperatively, linear lymphatic patterns were visualized up to the knee in all patients, but dermal backflow patterns could not be visualized in 83% from the level of the knee to the groin. Still, 67% demonstrated inguinal nodal uptake (mean transit 24 min), suggesting preserved lymphatic transport. All cases achieved intraoperative confirmation of intact lymphatic flow after debulking. The mean liposuction aspirate was 925 ± 250 mL per lower extremity; the mean excision mass was 2209 ± 757 g per lower extremity. Complications included two superficial cellulitis events (25%) and one wound dehiscence (12.5%); no hematomas or skin necrosis occurred. No patient developed clinical or imaging evidence of iatrogenic lymphedema during follow-up. Conclusions: Intraoperative ICG lymphography is a practical adjunct for lymphatic-sparing debulking of symptomatic lipedema, enabling real-time identification and preservation of superficial collectors while addressing focal lobules. This hybrid approach—targeted tumescent liposuction followed by ICG-guided superficial dermato-lipectomy—was associated with meaningful symptom improvement and a low morbidity in this early series. Full article

Pyruvate:ferredoxin oxidoreductase (PFOR) is a key Achilles’ heel in anaerobic pathogens. We integrate electronic-structure calculations (DFT), cheminformatic QSAR metrics, and residue-resolved docking to distill a concise “recognition code” and translate it into practical design rules. Using nitazoxanide (Nita; ΔG_(bind) ≈ −10.0 kcal·mol⁻¹) as a well-established reference, productive binding requires a conserved triad: a hydrogen-bond donor addressing Thr-997 and Cys-840, a π–π stack with Phe-869, and a recurrent π–σ contact to Thr-997 that orients the scaffold. Deacetylation to tizoxanide unmasks the phenolic donor and raises local electrophilicity, yet it also slightly loosens pocket packing (−9.6 kcal·mol⁻¹). Strategic halogenation introduces a σ-hole interaction near Pro-29, tightening pose geometry without disrupting the donor network; the lead analogue yields −10.1 kcal·mol⁻¹, and two others match the reference by preserving the triad and hydrophobic belt. The result is a minimal, testable recipe—retain the phenolic donor, enforce Thr-997/Cys-840 and Phe-869, and add a calibrated halogen σ-hole—offering falsifiable predictions to surpass nitazoxanide and guiding synthesis and biophysical validation in targeted PFOR inhibition. Full article