Search Results (69)

The essential oil derived from the bark of Cinnamomum zeylanicum L., Lauraceae, has gained significant attention because of its numerous biological benefits. This study aimed to perform a phytochemical analysis of commercially available Cinnamomum zeylanicum bark essential oil and to evaluate its antioxidant, antimicrobial, immunomodulatory, and antitumor properties. GC–MS analysis was employed to determine the phytochemical composition. The major component of the total essential oil composition was (E)-cinnamaldehyde, constituting 77.93%, followed by eugenol (4.34%), E-caryophyllene (3.68%), and linalool (2.79%). The antioxidant activity was confirmed by DPPH, ABTS, CUPRAC, and TAC assays. In the broth microdilution assay, cinnamon essential oil demonstrated strong antimicrobial activity, with MIC values ranging from 7.37 to 29.50 µg/mL. Furthermore, cinnamon essential oil demonstrated selective antitumor activity by inducing apoptosis and cell-cycle arrest in human colorectal cancer cells (HCT116) while sparing non-cancerous cells (MRC-5). In HCT116 cells, cinnamon essential oil induced apoptosis, downregulated Cyclin D and p-AKT, and caused G1-phase arrest. Additionally, cinnamon essential oil modulated immune responses by reducing pro-inflammatory cytokine production in activated splenocytes and enhancing pro-inflammatory activity in naïve cells. These findings highlight the great potential of the cinnamon bark essential oil in the development of new therapeutic agents. Full article

The experiment was designed to evaluate the consequence of a blend of garlic oil and cinnamon bark powder administration on growth performance, nutrient digestibility, immunity, antioxidant status and methane emission in Murrah buffalo (Bubalus bubalis). Sixteen buffalo calves were divided into two groups in a completely randomised design. The first group (CONT) was fed a basal diet of wheat straw, green oats and concentrate mixture, whereas the second group (GOCB) received feeds as per the CONT along with a blend of garlic oil and cinnamon bark powder (0.5 mL + 1.0 g/head/day) by mixing it with the concentrate mixture for a period of 170 days. The growth rate and feed efficiency in GOCB group buffalo calves were improved (20%) with better (p < 0.05) digestibility of organic matter and crude proteins. Buffaloes of the GOCB group revealed enhanced (p < 0.05) immunity and antioxidant enzymes with reduced (p < 0.05) lipid peroxidation (26% less MDA production). The methane concentration in the eructed gas of the GOCB buffaloes was reduced (33.88%) in comparison with the CONT (p < 0.01). Thus, feed formulated with a blend of garlic oil-cinnamon bark powder demonstrates improvements in the health and production performances of buffalo calves. Full article

The stabilization of essential oils in emulsions using surfactants of natural origin is of significant interest, and the use of biosurfactants produced by lactic acid bacteria could be an alternative. In this study, the total and partial substitution of Tween 80 in cinnamon bark essential oil emulsions was proposed using a glycolipopeptide biosurfactant produced by Lactiplantibacillus plantarum Tw226. The oil-in-water emulsions formulated contained cinnamon bark oil at a concentration of 5 g/L, with Tween 80, the biosurfactant, or a mixture of both as the surfactant agent, reaching a final concentration of 5 g/L. Homogenization was performed using a high-speed homogenizer. The emulsion with both the biosurfactant and Tween 80 was classified as a nanoemulsion (Z-av < 200 nm) that was stable for eight weeks, while the one with only the biosurfactant was a mini-emulsion (200 > Z-av < 500 nm). Furthermore, the emulsion with a combination of surfactants exhibited antioxidant activity equal to that of the emulsion with only Tween 80 and higher than that of the emulsion with only the biosurfactant. The antifungal activities of the three emulsions against Candida tropicalis, Candida krusei, and Zygosaccharomyces bailii did not change, regardless of the surfactant used, according to MIC values. In conclusion, a mixture of biosurfactant and Tween 80 or biosurfactant alone is an alternative for reducing or substituting synthetic surfactants in essential cinnamon bark oil emulsions, depending on their desired physical and functional properties. This work amplifies the scarce knowledge of essential oil emulsions stabilized with biosurfactants produced by lactic acid bacteria. Full article

Otitis externa is common infection in dogs. Its most important aetiologic agent is Pseudomonas aeruginosa, which, besides its ability to form biofilm, can also be resistant to several antibiotics, leading to therapeutic failures in several cases. Promising therapeutic alternatives are constantly being sought to treat chronic, recurrent infections. Within the framework of this study, we investigated the antibacterial potential of essential oils against canine isolates of P. aeruginosa. Forty P. aeruginosa were isolated from individual dogs’ otitis externa. They were identified by MALDI-TOF MS, their antibiotic susceptibility was determined with disk diffusion, and their genetic relatedness was established using pulsed-field gel electrophoresis. Fifty-seven essential oils were screened for their antipseudomonas effects using the drop plate method, and six essential oils—cinnamon (bark and leaf), bay, rosemary, thyme, and bitter orange—were further tested for their mature-biofilm-degrading capacities at 30 °C and 37 °C. Even though the molecular relatedness of the forty P. aeruginosa isolates could be excluded, all isolates’ growth was inhibited and their biofilms degraded by a 2.5% concentration of cinnamon (bark and leaf), bay, rosemary, thyme, or bitter orange essential oil. Our results show that essential oils are potent alternatives in the treatment of otitis externa. Full article

The potato (Solanum tuberosum L.) is one of the most widely consumed vegetable crops worldwide. During storage, potato tubers are vulnerable to various phytopathogenic fungi. Dry rot, caused by Fusarium incarnatum, is a common and serious disease that affects potato tubers, leading to partial or complete decay during storage. The current study assessed the effectiveness of three ethanolic extracts including cinnamon bark (CIB), clove buds (CLB), and avocado seeds (AVS) in controlling potato dry rot under both normal and cold storage conditions. In vitro bioassay demonstrated that all tested extracts exhibited a dose-dependent fungistatic effect against F. incarnatum, with inhibition percentages of 83.33% for CIB, 72.22% for CLB, and 67.77% for AVS at the highest tested concentration. Moreover, dipping potato tubers in the tested extracts markedly reduced the severity of dry rot disease under both normal and cold storage conditions. Additionally, treated tubers showed increased activities of defense-related enzymes, including catalase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase. Furthermore, there were higher levels of total soluble phenolics and flavonoids, along with an increase in lignin content and a reduction in the weight loss of stored potato tubers compared to the control group. Moreover, the extracts mitigated infection stress and lowered malondialdehyde levels in the treated potato tubers. These extracts show potential as environmentally friendly alternatives to chemical fungicides for managing potato dry rot caused by F. incarnatum under normal and cold storage. Full article

Background/Objectives: This study aimed to develop cinnamon bark essential oil (CEO), orange peel essential oil(OEO) and the combination of these two essential oils (OEO-CEO) loaded PLGA nanoparticles to prevent dental caries and to investigate their effectiveness in silico and in vitro methods. Methods: EO loaded PLGA nanoparticles were produced by single emulsion method. Detailed characterization studies were performed using different methods, and the controlled release profile was obtained. The antibacterial activity of the developed formulations was investigated on S. mutans and L. casei strains by in vitro and in silico methods. Additionally, the interaction mechanisms of EOs with DNA were evaluated. Results: Our findings showed that the average droplet size of EO-loaded PLGA nanoparticles varied between 243.1 ± 0.60 nm and 219 ± 4.49 nm, while PdI values varied between 0.069 ± 0.039 and 0.032 ± 0.01. In addition, the developed nanoparticles had high encapsulation efficiency (85.14% to 66.28%) and released the active ingredient in a continuous and controlled manner. Ames test showed that the genotoxicity of EOs was eliminated due to the encapsulation of EOs in PLGA nanoparticles and antibacterial tests showed that OEO-CEO-loaded PLGA nanoparticles were effective on L. casei and S. mutans. The antibacterial activity of EOs was also supported by in silico studies. Finally, it was revealed that EOs showed potential as antibacterial agents by interacting with DNA. Conclusions: The results showed that OEO-CEO-loaded PLGA nanoparticles have the potential to be a suitable nanoformulation for developing mouthwash or toothpaste for the prevention and treatment of dental caries. Full article

This study evaluated the potential usage of phosphorylated egg white protein (P-EWP) nanogels fabricated via microwave-induced phosphorylation modification and gel process and further ultrasonic nanometrization as novel delivery systems for cinnamon bark essential oil (CBEO). Compared to EWP-CBEO nanogels without chemical phosphorylation, the obtained P-EWP-CBEO nanogels have shown smaller average hydrodynamic diameter (133.6 nm), relatively uniform size distribution (polydispersity index around 0.265), enhanced negative surface charge (−35.4 mV), and improved stability under the conditions of high temperature (up to 90 °C) and ionic strength (up to 200 mM NaCl). Moreover, P-EWP-CBEO nanogels, with hydrophobic interactions and disulfide bonds as the main intermolecular forces, exhibited a remarkable conformational change in microstructures. In addition, the results of the antibacterial experiments on Escherichia coli, Staphylococcus aureus, and Listeria monocytogenes showed that the MIC values of P-EWP-CBEO nanogels were two times lower than those of EWP-CBEO nanogels and could completely inhibit the growth of pathogenic bacteria within 108 h. Hence, we have suggested that P-EWP-CBEO nanogels are successfully fabricated with improved physicochemical properties as novel potential natural preservatives in the food industry. Full article

In recent years, attention has been paid to finding eco-friendly products that could represent an alternative for the chemicals usually used during conservation procedures. In the field of the devitalization of biological patinas, the most studied products are essential oils (EOs). Several works tested EOs in vitro on microorganisms isolated from stone artifacts but few applied these products on real artworks colonized by a complex patina. In the present work, the biocidal effect of two EOs (cinnamon bark and oregano) and two EO-based products (Biotersus and Essenzio) was compared to that of three chemical biocides commonly used in the conservation of stone artifacts (Biotin R1+R2, NewDes50, and Preventol RI50). The products were applied by brush in situ on a marble slab characterized by a green patina. The biocidal activity was evaluated by analyzing the chlorophyll fluorescence and quantifying ATP through biochemical tests. Furthermore, the products’ interference with stone was evaluated by colorimetric measures on sedimentary rock samples. The results indicate that chemical biocides exhibited biocidal activity of greater than 99.5% with a single application, while cinnamon oil (at a 1% concentration) and Essenzio required two applications to achieve the same level of efficacy. Conversely, oregano oil and Biotersus were not able to effectively reduce microorganism vitality, even with repeated applications. Full article

The influence of essential oils (EOs) on gut microorganisms and broiler chicken production was evaluated through the systematic analysis of scientific reports. The present study was focused on the EO antimicrobial activity oriented toward broiler chicken production. There is a great biodiversity of plants, and various compounds with different biological activity have been isolated from them. The EO molecules extracted from plants have been employed recently in livestock feeding. Microbial resistance to antibiotics has led to their reduced use in animal production. To maintain competitive broiler chicken production with reduced antibiotic use, EOs have been explored. In broiler chickens, EOs are supplemented in the diet or drinking water to enhance weight gain and feed efficiency and reduce mortality. EOs are an alternative to antibiotics, and their research is dynamic in poultry production. The present review focused on the antimicrobial activity oriented to broiler chicken production. The search for information in databases used the terms “broiler chicken”, “essential oils” and combined them with the name of the plants. It was detected that the EO of Cinnamon bark or its compound cinnamaldehyde could reduce pathogenic bacteria in the digestive tract and improve intestinal morphology. Essential oils from Cymbopogon spp. and Origanum vulgare had an effect mainly against Gram-negative bacteria, such as Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella spp., and others, and against some Gram-positive bacteria, such as Staphylococcus spp., or yeasts, such as Candida albicans. Essential oils of Cymbopogon citratus acted against Salmonella. Citrus japonica affected Bacillus subtilis, Escherichia coli, and Salmonella typhimurium. Origanum EO improved the antioxidant status and gut health of chickens, while EO of Eucalyptus with carvacrol, thymol, and Citrus lemon improved the productive performance of broiler chickens; also, Citrus spp. reduced the number of oocysts of Eimeria and showed activity against Listeria monocytogenes, Staphylococcus aureus, and Pseudomonas aeruginosa. It is concluded that EOs are a sustainable alternative to antibiotics in the production of broiler chickens. Future research includes the standardization of EO from different plants and active molecules, as well as the interaction with other feed additives and their impact on the health and production of broiler chickens. In addition, safety for consumers and the environment must be considered. Full article

In recent years, essential oils (EOs) have received increased attention from the research community, and the EOs of cinnamon, patchouli, and geranium have become highly recognized for their antibacterial, antifungal, antiviral, and antioxidant effects. Due to these properties, they have become valuable and promising candidates for addressing the worldwide threat of antimicrobial resistance and other diseases. Simultaneously, studies have revealed promising new results regarding the effects of physical fields (magnetic and electric) and LASER (MEL) exposure on seed germination, plant growth, biomass accumulation, and the yield and composition of EOs. In this frame, the present study aims to investigate the influence of MEL treatments on cinnamon, patchouli, and geranium EOs, by specifically examining their composition, antimicrobial properties, and antioxidant activities. Results showed that the magnetic influence has improved the potency of patchouli EO against L. monocytogenes, S. enteritidis, and P. aeruginosa, while the antimicrobial activity of cinnamon EO against L. monocytogenes was enhanced by the electric and laser treatments. All exposures have increased the antifungal effect of geranium EO against C. albicans. The antioxidant activity was not modified by any of the treatments. These findings could potentially pave the way for a deeper understanding of the efficiency, the mechanisms of action, and the utilization of EOs, offering new insights for further exploration and application. Full article

Synthesis of silver nanoparticles with antibacterial properties using a one-pot green approach that harnesses the natural reducing and capping properties of cinnamon (Cinnamomum verum) bark extract is presented in this work. Silver nitrate was the sole chemical reagent employed in this process, acting as the precursor salt. Gas Chromatography-Mass Spectroscopy (GC-MS), High-Performance Liquid Chromatography (HPLC) analysis, and some phytochemical tests demonstrated that cinnamaldehyde is the main component in the cinnamon bark extract. The resulting bio-reduced silver nanoparticles underwent comprehensive characterization by Ultraviolet–Vis (UV-Vis) and Fourier Transform InfraRed spectrophotometry (FTIR), Dynamic Light Scattering (DLS), Transmission Electron Microscopy, and Scanning Electron Microscopy suggesting that cinnamaldehyde was chemically oxidated to produce silver nanoparticles. These cinnamon-extract-based silver nanoparticles (AgNPs-cinnamon) displayed diverse morphologies ranging from spherical to prismatic shapes, with sizes spanning between 2.94 and 65.1 nm. Subsequently, the antibacterial efficacy of these nanoparticles was investigated against Klebsiella, E. Coli, Pseudomonas, Staphylococcus aureus, and Acinetobacter strains. The results suggest the promising potential of silver nanoparticles obtained (AgNPs-cinnamon) as antimicrobial agents, offering a new avenue in the fight against bacterial infections. Full article

Avian pathogenic Escherichia coli (APEC) causes a variety of infections outside the intestine. The treatment of these infections is becoming increasingly difficult due to the emergence of multi-drug resistant (MDR) strains, which can also be a direct or indirect threat to humans as consumers of poultry products. Therefore, alternative antimicrobial agents are being sought, which could be essential oils, either administered individually or in interaction with antibiotics. Sixteen field isolates of E. coli (originating from 1-day-old broilers) and the ATCC 25922 reference strain were tested. Commercial cinnamon bark, clove bud, lavender flower essential oils (EOs) and enrofloxacin were selected to assess the sensitivity of the selected E. coli strains to antimicrobial agents. The checkerboard method was used to estimate the individual minimum inhibitory concentration (MIC) for each antimicrobial agent as well as to determine the interactions between the selected essential oil and enrofloxacin. In the case of enrofloxacin, ten isolates were resistant at MIC ≥ 2 μg/mL, three were classified as intermediate (0.5–1 μg/mL) and three as sensitive at ≤0.25 μg/mL. Regardless of the sensitivity to enrofloxacin, the MIC for cinnamon EO was 0.25% v/v and for clove EO was 0.125% v/v. All MDR strains had MIC values for lavender EO of 1% v/v, while drug-sensitive isolates had MIC of 0.5% v/v. Synergism between enrofloxacin and EO was noted more frequently in lavender EO (82.35%), followed by cinnamon EO (64.7%), than in clove EO (47.1%). The remaining cases exhibited additive effects. Owing to synergy, the isolates became susceptible to enrofloxacin at an MIC of ≤8 µg/mL. A time–kill study supports these observations. Cinnamon and clove EOs required for up to 1 h and lavender EO for up to 4 h to completely kill a multidrug-resistant strain as well as the ATCC 25922 reference strain of E. coli. Through synergistic or additive effects, blends with a lower than MIC concentration of enrofloxacin mixed with a lower EO content required 6 ± 2 h to achieve a similar effect. Full article

Porous wood-based boards, like any lignocellulosic material, are susceptible to biocorrosion caused by mold fungi. Their durability can be extended by using biocides. Due to the fact that porous boards are considered an ecological material, it would be beneficial to also use natural agents to protect them. For this purpose, the surface of softboards was protected with a 30% solution of cinnamon bark oil in ethanol. Three application levels were used: 75 g/m², 120 g/m², and 200 g/m² of solution. It has been shown that the cinnamon bark oil solution used at an application rate of 200 g/m² is an effective fungicide, protecting softboards (SBs) against the development of mold fungi: T. viride and C. globosum. The dominant volatile component of cinnamon oil identified in the boards turned out to be cinnamaldehyde. Three months after treatment, this substance constituted 74% of the volatile components. The proposed treatment method allows for short-term preventive protection of boards against mold fungi. Full article

Penicillium digitatum is a major postharvest pathogen that threatens the global citrus fruit industry and causes great economic losses annually. In the present study, inhibitory properties of cinnamon bark oil (CBO) against P. digitatum in vitro were investigated. Results indicated that 0.03% CBO could efficiently inhibit the spore germination, germ tube elongation, mycelial growth, colonial expansion and conidial accumulation of P. digitatum. The results of fluorescein diacetate (FDA) and MitoTraker Orange (MTO) staining also proved the suppression effects of CBO against P. digitatum. Meanwhile, CBO could inhibit green mold rots induced by P. digitatum in citrus fruit when the working concentration of CBO exceeded 0.06%. In addition, the expressions of 12 genes critical for the growth and virulence of P. digitatum were also significantly regulated under CBO stress. Through a transcriptomic analysis, a total of 1802 common differentially expressed genes (DEGs) were detected in P. digitatum after 4 h and 8 h of CBO treatment. Most of the DEG products were associated with carbohydrate, amino acid and lipid metabolism. They directly or indirectly led to the disturbance of the membrane and the generation of reactive oxygen species (ROS). Our results may deepen the understanding of antifungal properties of CBO against P. digitatum and provide the theoretical foundation to uncover the antifungal mechanism of CBO at the molecular level. Full article

Cinnamomum verum (syn C. zeylanicum) is considered ‘true’ cinnamon. However, it is reported that less expensive sources of cinnamon from C. cassia (syn C. aromaticum), C. loureiroi, and C. burmannii (toxic coumarin) may be used in the place of C. verum. We lack the quality assurance tools that are required to differentiate C. verum from other cinnamon species when verifying that the correct species is sourced from ingredient suppliers. The current research on cinnamon species authentication using DNA tools is limited to a few species and the use of high-quality DNA extracted from raw leaf materials. The cinnamon bark traded in the supply chain contains much less DNA and poorer-quality DNA than leaves. Our research advances DNA methods to authenticate cinnamon, as we utilized full-length chloroplast genomes via a genome skimming approach for C. burmannii and C. cassia to facilitate the design of optimal mini DNA markers. Furthermore, we developed and validated the use of NMR fingerprints for several commercial cinnamon species, including the quantification of 16 molecules. NMR fingerprints provided additional data that were useful for quality assessment in cinnamon extract powders and product consistency. Both the new mini DNA markers and NMR fingerprints were tested on commercial cinnamon products. Full article