Search Results (2,123)

Insect pests cause severe damage and yield losses to many agricultural crops globally. The use of chemical pesticides on agricultural crops is not recommended because of their toxic effects on the environment and consumers. In addition, pesticide toxicity reduces soil fertility, poisons ground waters, and is hazardous to soil biota. Therefore, applications of entomopathogenic nematodes (EPNs) and plant growth-promoting rhizobacteria (PGPR) are an alternative, eco-friendly solution to chemical pesticides and mineral-based fertilizers to enhance plant health and promote sustainable food security. This review focuses on the biological and ecological aspects of these organisms while also highlighting the practical application of molecular communication approaches in developing a novel plant health product. This insight will support this innovative approach that combines PGPR and EPNs for sustainable crop production. Several studies have reported positive interactions between nematodes and bacteria. Although the combined presence of both organisms has been shown to promote plant growth, the molecular interactions between them are still under investigation. Integrating molecular communication studies in the development of a new product could help in understanding their relationships and, in turn, support the combination of these organisms into a single plant health product. Full article

Concurrent cognitive tasks, such as avoiding visual, auditory, chemical, and electrical hazards, and concurrent motor tasks, such as load carriage, are prevalent in ergonomic settings. Trips are extremely common in the workplace, leading to fatal and non-fatal fall-related injuries. Intrinsic factors, such as attention, fatigue, and anticipation, as well as extrinsic factors, including tasks at hand, affect trip recovery responses. Objective: The purpose of this study was to investigate the ankle joint kinematics in unexpected and expected trip responses during single-tasking (ST), dual-tasking (DT), and triple-tasking (TT), before and after a physically fatiguing protocol among young, healthy adults. Methods: Twenty volunteers’ (10 females, one left leg dominant, age 20.35 ± 1.04 years, height 174.83 ± 9.03 cm, mass 73.88 ± 15.55 kg) ankle joint kinematics were assessed using 3D motion capture system during unperturbed gait (NG), unexpected trip (UT), and expected trip (ET), during single-tasking (ST), cognitive dual-tasking (CDT), motor dual-tasking (MDT), and triple-tasking (TT), under both PRE and POST fatigue conditions. Results: Greater dorsiflexion angles were observed during UT compared to NG, MDT compared to ST, and TT compared to ST. Significantly greater plantar flexion angles were observed during ET compared to NG and during POST compared to PRE. Conclusions: Greater dorsiflexion angles during dual- and triple-tasking suggest that divided attention affects trip recovery. Greater plantar flexion angles following fatigue are likely an anticipatory mechanism due to altered muscle activity and increased postural control demands. Full article

Deformed wing virus (DWV) is a major contributor to honey bee colony losses, making effective monitoring essential for apiary management. Traditional DWV detection relies on hazardous RNA extraction followed by RT-qPCR, creating barriers for widespread surveillance. We developed an immunocapture RT-qPCR (IC-RT-PCR) method for screening DWV-A infections by capturing intact virus particles from bee homogenates using immobilized antibodies. Validation demonstrated strong correlation with TRIzol^®-based extraction (r = 0.821), with approximately 6 Ct reduced sensitivity, consistent with other published immunocapture methods. Performance was adequate for moderate–high viral loads, while TRIzol^® showed superior detection for low-dose infections. Laboratory-produced reverse transcriptase showed equivalent performance to commercial enzymes, providing cost savings. IC-RT-PCR eliminates hazardous chemicals and offers a streamlined workflow for surveillance screening where the safety and cost benefits outweigh the sensitivity reduction. This method provides a practical alternative for large-scale DWV-A surveillance programs, while TRIzol^® remains preferable for low-level detection and diagnostic confirmation. Full article

Microplastics are an increasing concern due to their widespread occurrence in aquatic environments worldwide. The lack of a harmonised protocol for their reliable quantification remains a major challenge in current scientific efforts. This study presents a comparative evaluation of three protocols for the detection and quantification of microplastics in aqueous samples. The protocols were assessed based on quantification efficiency, risk of particle degradation, staining performance, operational complexity, and cost per sample. Protocol A combined Rhodamine B and ethanol staining with NaCl-based density separation, demonstrating strong isolation performance while maintaining minimal chemical hazards and moderate cost (2.45€ per sample) that could be further reduced to 0.45€ per sample by substituting reagent-grade NaCl with table salt. Protocol B offered moderate isolation capacity and presented the highest risk of particle fragmentation, likely due to the use of acetone and high-temperature digestion. Protocol C, based on the combined use of Nile Red and ZnCl₂, also presented a risk of particle fragmentation, resulting in the highest MP count for small and hydrophobic particles. In addition, its high cost (15.23€ per sample) limits its suitability for routine application. Full article

Precise odor source localization in turbulent and sparse environments plays a vital role in enabling robotic systems for hazardous chemical monitoring and effective disaster response. To address this, we propose Cooperative Gravitational-Rényi Infotaxis (CGRInfotaxis), a distributed decision-optimization framework that combines multi-agent collaboration with hybrid cognitive strategy to improve search efficiency and robustness. The method integrates a gravitational potential field for rapid source convergence and Rényi divergence-based probabilistic exploration to handle sparse detections, dynamically balanced via a regulation factor. Particle filtering optimizes posterior probability estimation to autonomously refine search areas while preserving computational efficiency, alongside a distributed interactive-optimization mechanism for real-time decision updates through agent cooperation. The algorithm’s performance is evaluated in scenarios with fixed and randomized odor source locations, as well as with varying numbers of agents. Results demonstrate that CGRInfotaxis achieves a near-100% success rate with high consistency across diverse conditions, outperforming existing methods in stability and adaptability. Increasing the number of agents further enhances search efficiency without compromising reliability. These findings suggest that CGRInfotaxis significantly advances multi-agent odor source localization in turbulent, sparse environments, offering practical utility for real-world applications. Full article

Allergic contact dermatitis (ACD) is an immunologic reaction to a dermal chemical exposure that, once triggered in an individual, will result in an allergic response following subsequent encounters with the allergen. Air Force epidemiological consultations have indicated that aircraft structural maintenance workers may experience ACD at elevated rates compared to other occupations. We aimed to better understand the utility of non-animal testing methods in characterizing the sensitization potential of chemicals used during Air Force operations by evaluating the skin sensitization hazard of Air Force-relevant chemicals using new approach methodologies (NAMs) in a case study. We also evaluated the use of NAM data to develop preliminary candidate surface guidelines (PCSGs, maximum concentrations of chemicals on workplace surfaces to prevent induction of dermal sensitization) for chemicals identified as sensitizers. NAMs for assessing skin sensitization, including in silico models and experimental assays, were leveraged into an integrated approach to predict sensitization hazard for 19 chemicals. Local lymph node assay effective concentration values were predicted from NAM assay data via previously published quantitative models. The derived values were used to calculate PCSGs, which can be used to compare the presence of these chemicals on work surfaces to better understand the risk of Airmen developing ACD from occupational exposures. Full article

The utilisation of edible insects for human nutrition is a long-standing practice in many parts of the globe, and is being gradually introduced into countries without an entomophagic tradition as well. These unconventional sources of protein of animal origin have arisen as a sustainable alternative to other animal protein sources, such as meat. This review intends to present the compilation of data in the scientific literature on the chemical composition and nutritional value of the bee brood of A. mellifera species and subspecies as edible foods. For this, a comprehensive search of the scientific literature was carried out using the databases ScienceDirect, Scopus, Pub-Med, BOn, and SciELO. Appropriate keywords were used for the search to reach the research works that addressed the topics of the review. The results showed that bee brood has considerable quantities of protein, fat and carbohydrates. The most abundant amino acids are leucine and lysine (these two being essential amino acids) and aspartic acid, glutamic acid, and proline (these three being non-essential amino acids). As for the fatty acids, bee broods contain approximately equal fractions of saturated and monounsaturated fatty acids, while the polyunsaturated fatty acids are negligible. The dietary minerals present in higher quantities are potassium, phosphorus, and magnesium, and the most abundant vitamins are vitamin C and niacin; choline is also present, although it is not a true vitamin. Although bee brood from A. mellifera has potential for human consumption as a nutrient-rich food, there are still many aspects that need to be further studied in the future, such as safety and hazards linked to possible regular consumption. Full article

Entomopathogenic fungi are among the most promising non-chemical alternatives for the control of many serious phytophagous insect pests, such as moth species. The present research investigates the use of the little-studied entomopathogenic fungus Beauveria varroae as a biocontrol agent against the notorious pests Helicoverpa armigera and Sesamia nonagrioides in laboratory conditions. Conidial suspensions of B. varroae were prepared at 10³-10⁴-10⁵-10⁶-10⁷-10⁸ conidia/mL to assess their insecticidal potential. In this study, we used 100 3rd-instar larvae for each concentration. During the lab bioassays, almost complete mortality of 35–96.6% was recorded for H. armigera larvae and 40–96.6% for S. nonagrioides larvae 10 days after exposure. The lethal effect of the entomopathogen was related to both dose and exposure time of the entomopathogen, with higher concentrations resulting in increased mortality. The survival effect of S. nonagrioides and H. armigera larvae was dependent on the hazard effect of the used dose and the exposure time. These findings indicate that B. varroae has potential as a biocontrol agent. Further research will elucidate this new isolate and optimize application methods in field conditions. Full article

A scalable synthesis of two-dimensional titanium nitride chloride (TiNCl) via flash Joule heating (FJH) using titanium tetrachloride (TiCl₄) precursor has been developed. This single-step method overcomes traditional synthesis challenges, including high energy consumption, multi-step procedures, and hazardous reagent requirements. The structural and chemical properties of the synthesized TiNCl were characterized through multiple analytical techniques. X-ray diffraction (XRD) patterns confirmed the presence of TiNCl phase, while Raman spectroscopy data showed no detectable oxide impurities. Fourier transform infrared spectroscopy (FTIR) analysis revealed characteristic Ti–N stretching vibrations, further confirming successful titanium nitride synthesis. Transmission electron microscopy (TEM) imaging revealed thin, plate-like nanostructures with high electron transparency. These analyses confirmed the formation of highly crystalline TiNCl flakes with nanoscale dimensions and minimal structural defects. The material exhibits excellent structural integrity and phase purity, demonstrating potential for applications in photocatalysis, electronics, and energy storage. This work establishes FJH as a sustainable and scalable approach for producing MXenes with controlled properties, facilitating their integration into emerging technologies. Unlike conventional methods, FJH enables rapid, energy-efficient synthesis while maintaining material quality, providing a viable route for industrial-scale production of two-dimensional materials. Full article

The integration of nanotechnology and green synthesis strategies provides innovative solutions in biomedicine. This study focuses on the biofabrication of silver nanoparticles (AgNPs) using Corynespora smithii, an endophytic fungus isolated from Bergenia ciliata. The eco-friendly synthesis process employed fungal extracts as reducing and stabilizing agents thereby minimizing the need for hazardous chemicals. The AgNPs demonstrated strong potent biological activities, showcasing significant antioxidant, antibacterial, and anticancer properties. The antibacterial efficacy was demonstrated against various Gram-positive and Gram-negative bacteria, while cytotoxicity on the A549 lung cancer cell line revealed an IC₅₀ value of 10.46 µg/mL. A molecular docking analysis revealed interactions between the major bioactive compound, dimethylsulfoxonium formylmethylide, and the pathogenic proteins, Staphylococcus aureus and Salmonella typhi, displaying moderate binding affinities. Furthermore, the ADME analysis of dimethylsulfoxonium formylmethylide indicated favourable pharmacokinetic properties, including high gastrointestinal absorption, minimal lipophilicity, and low potential for drug–drug interactions, making it a promising candidate for oral drug formulations. These findings further support the compound’s suitability for biomedical applications. This research emphasizes the potential of C. smithii as a sustainable source for synthesizing bioactive nanoparticles, paving the way for their application in developing novel therapeutic agents. This study highlights the significance of harnessing endophytic fungi from medicinal plants for sustainable nanotechnology advancements. Full article

The contamination of drinking water sources with selenium (Se) oxyanions, including selenite (Se(IV)) and selenate (Se(VI)), contains serious health hazards with an oral intake exceeding 400 µg/day and therefore requires urgent attention. Various natural and anthropogenic sources are responsible for high Se concentrations in aquatic environments. In addition, the chemical behavior and speciation of selenium can vary noticeably depending on the origin of the source water. The Se(VI) oxyanion is more soluble and therefore more abundant in surface water. Se levels in contaminated waters often exceed 50 µg/L and may reach several hundred µg/L, well above drinking water limits set by the World Health Organization (40 µg/L) and Germany (10 µg/L), as well as typical industrial discharge limits (5–10 µg/L). Overall, Se is difficult to remove using conventionally available physical, chemical, and biological treatment technologies. The recent literature has therefore highlighted promising advancements in Se removal using emerging technologies. These include advanced physical separation methods such as membrane-based treatment systems and engineered nanomaterials for selective Se decontamination. Additionally, other integrated approaches incorporating photocatalysis coupled adsorption processes, and bio-electrochemical systems have also demonstrated high efficiency in redox transformation and capturing of Se from contaminated water bodies. These innovative strategies may offer enhanced selectivity, removal, and recovery potential for Se-containing species. Here, a current review outlines the sources, distribution, and chemical behavior of Se in natural waters, along with its toxicity and associated health risks. It also provides a broad and multi-perspective assessment of conventional as well as emerging physical, chemical, and biological approaches for Se removal and/or recovery with further prospects for integrated and sustainable strategies. Full article

Alternative proteins denote non-traditional, high-protein foods. These innovative sources aim to compete with conventional animal products by providing protein-rich, sustainable, nutritious, and flavorful options. Currently, five main categories of alternative proteins are being developed: plant-based proteins, cultured meat, single-cell proteins, edible insects, and seaweed. Nonetheless, several chemical and microbiological food safety hazards are associated with these alternatives Incorporating novel protein sources into food products may heighten the prevalence of existing food allergies. This could arise from extracting proteins from their natural matrices and utilizing them at significantly higher concentrations. Additionally, the introduction of new proteins may lead to the development of novel food allergies. Proteins that are currently seldom or never consumed may cause primary sensitisation or trigger cross-reactivity with known allergens. To date, alternative proteins have not been thoroughly studied for their allergenic potential, and there is no standardised method for assessing this risk. This review aims to explore non-traditional protein sources, discussing their nutritional and functional properties, as well as their potential allergenicity based on available research. We conducted a literature search in PubMed and Embase databases. We used specific keywords and MESH terms. A total of 157 studies were included in the review. The studies reviewed in our analysis reveal significant limitations, such as inconsistent methodologies, limited participant numbers, and a lack of long-term data, which hinder the ability to make clear conclusions regarding the safety of these new proteins for individuals with allergies. To address current challenge, future research should integrate food science, regulatory perspectives and advanced technologies. Full article

Infant clothing represents a critical yet overlooked exposure pathway for heavy metals, with significant implications for child health and sustainable consumption. This study investigates cadmium (Cd) and chromium (Cr) contamination in 33 textile samples, integrating in vitro bioaccessibility assays, cytotoxicity analysis, and risk assessment models to evaluate dermal exposure risks. Results reveal that 80% of samples exceeded OEKO-TEX Class I limits for As (mean 1.01 mg/kg), Cd (max 0.25 mg/kg), and Cr (max 4.32 mg/kg), with infant clothing showing unacceptable hazard indices (HI = 1.13) due to Cd (HQ = 1.12). Artificial sweat extraction demonstrated high bioaccessibility for Cr (37.8%) and Ni (28.5%), while keratinocyte exposure triggered oxidative stress (131% ROS increase) and dose-dependent cytotoxicity (22–59% viability reduction). Dark-colored synthetic fabrics exhibited elevated metal loads, linking industrial dye practices to health hazards. These findings underscore systemic gaps in textile safety regulations, particularly for low- and middle-income countries reliant on cost-effective apparel. We propose three policy levers: (1) tightening infant textile standards for Cd/Cr, (2) incentivizing non-toxic dye technologies, and (3) harmonizing global labeling requirements. By bridging toxicological evidence with circular economy principles, this work advances strategies to mitigate heavy metal exposure while supporting Sustainable Development Goals (SDGs) 3 (health), 12 (responsible consumption), and 12.4 (chemical safety). Full article

Many effluents from human activities discharged into freshwater ecosystems cause chemical pollution. Chemical pollution in rivers is a serious threat to freshwater ecosystems due to the associated potential human health risks. This study determined the extent of chemical pollution, identified potential sources of pollution and assessed human health risk in the Moopetsi River, an intermittent river in the Limpopo Province of South Africa. Chemical analyses were conducted on water and sediment samples collected during high-flow, low-flow and intermittent-flow regimes. The findings showed seasonal variations in the chemical pollution levels in the sediments and the highest contamination was measured during intermittent flow. The enrichment factor and geoaccumulation index values identified chromium and nickel as major contributors to sediment contamination. The mean arsenic, chromium and nickel levels exceeded the established guideline values. An evaluation of human health risk was conducted using ingestion and dermal absorption pathways. The results showed that ingestion has greater non-carcinogenic and carcinogenic risks than dermal exposure, especially for children during intermittent flow. The elements of great concern for non-carcinogenic risk were chromium, manganese and nickel and for carcinogenic risk, they were arsenic, chromium, nickel and lead. The outcome of this study is useful for waste management and conservation to reduce environmental degradation and human health risk. Full article

Mining waste presents significant environmental and public health risks due to the potential release of toxic substances when improperly managed. In this study, four tailings samples were taken to evaluate the environmental risks in the Ponce Enríquez mining area in Ecuador. Chemical characterization and X-ray Fluorescence Spectrometry (XRF) were used to analyze the content of potentially toxic elements (PTEs) of interest (As, Cd, Cr, Cu, Ni, Pb, and Zn), and X-ray Diffraction (XRD) for mineralogical characterization. The contamination index (IC) was calculated to assess the potential hazard associated with the content of PTEs in the mining wastes. To assess environmental risks, leaching tests were carried out to evaluate the potential release of PTEs, and Acid-Base Accounting (ABA) tests were conducted to determine the likelihood of acid mine drainage formation. The results revealed that the PETs concentration exceeded the maximum permissible limits in all samples, according to Ecuadorian regulations: As, Pb, and Cd were identified as critical contaminants. Mineralogically, quartz was the dominant phase, followed by carbonates (calcite, dolomite and magnesite), phyllosilicates (chlorite and illite), and minor amounts of pyrite and talc. The IC indicated high to very high contamination risk levels, with As being the predominant contributor. Although leaching tests met the established limits for non-hazardous mining waste, the ABA test showed that all samples had a high potential for long-term acid generation. These results underscore the need for implementing management strategies to mitigate the environmental impacts and the development of plans to protect local ecosystems and communities from the adverse effects of mining activities. Full article