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21 pages, 14026 KiB  
Article
Development of PEO in Low-Temperature Ternary Nitrate Molten Salt on Ti6Al4V
by Michael Garashchenko, Yuliy Yuferov and Konstantin Borodianskiy
Materials 2025, 18(15), 3603; https://doi.org/10.3390/ma18153603 - 31 Jul 2025
Viewed by 176
Abstract
Titanium alloys are frequently subjected to surface treatments to enhance their biocompatibility and corrosion resistance in biological environments. Plasma electrolytic oxidation (PEO) is an environmentally friendly electrochemical technique capable of forming oxide layers characterized by high corrosion resistance, biocompatibility, and strong adhesion to [...] Read more.
Titanium alloys are frequently subjected to surface treatments to enhance their biocompatibility and corrosion resistance in biological environments. Plasma electrolytic oxidation (PEO) is an environmentally friendly electrochemical technique capable of forming oxide layers characterized by high corrosion resistance, biocompatibility, and strong adhesion to the substrate. In this study, the PEO process was performed using a low-melting-point ternary eutectic electrolyte composed of Ca(NO3)2–NaNO3–KNO3 (41–17–42 wt.%) with the addition of ammonium dihydrogen phosphate (ADP). The use of this electrolyte system enables a reduction in the operating temperature from 280 to 160 °C. The effects of applied voltage from 200 to 400V, current frequency from 50 to 1000 Hz, and ADP concentrations of 0.1, 0.5, 1, 2, and 5 wt.% on the growth of titanium oxide composite coatings on a Ti-6Al-4V substrate were investigated. The incorporation of Ca and P was confirmed by phase and chemical composition analysis, while scanning electron microscopy (SEM) revealed a porous surface morphology typical of PEO coatings. Corrosion resistance in Hank’s solution, evaluated via Tafel plot fitting of potentiodynamic polarization curves, demonstrated a substantial improvement in electrochemical performance of the PEO-treated samples. The corrosion current decreased from 552 to 219 nA/cm2, and the corrosion potential shifted from −102 to 793 mV vs. the Reference Hydrogen Electrode (RHE) compared to the uncoated alloy. These findings indicate optimal PEO processing parameters for producing composite oxide coatings on Ti-6Al-4V alloy surfaces with enhanced corrosion resistance and potential bioactivity, which are attributed to the incorporation of Ca and P into the coating structure. Full article
(This article belongs to the Special Issue Microstructure Engineering of Metals and Alloys, 3rd Edition)
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14 pages, 1342 KiB  
Article
Mitigating Deicer-Induced Salinity Through Activated Carbon and Salt-Tolerant Grass Integration: A Case of Pennisetum alopecuroides
by Jae-Hyun Park, Hyo-In Lim, Myung-Hun Lee, Yong-Han Yoon and Jin-Hee Ju
Environments 2025, 12(7), 250; https://doi.org/10.3390/environments12070250 - 20 Jul 2025
Viewed by 580
Abstract
The use of chloride-based deicing salts, particularly sodium chloride (NaCl) and calcium chloride (CaCl2), is a common practice in cold regions for maintaining road safety during winter. However, the accumulation of salt residues in adjacent soils poses serious environmental threats, including [...] Read more.
The use of chloride-based deicing salts, particularly sodium chloride (NaCl) and calcium chloride (CaCl2), is a common practice in cold regions for maintaining road safety during winter. However, the accumulation of salt residues in adjacent soils poses serious environmental threats, including reduced pH, increased electrical conductivity (EC), disrupted soil structure, and plant growth inhibition. This study aimed to evaluate the combined effect of activated carbon (AC) and Pennisetum alopecuroides, a salt-tolerant perennial grass, in alleviating salinity stress under deicer-treated soils. A factorial greenhouse experiment was conducted using three fixed factors: (i) presence or absence of Pennisetum alopecuroides, (ii) deicer type (NaCl or CaCl2), and (iii) activated carbon mixing ratio (0, 1, 2, 5, and 10%). Soil pH, EC, and ion concentrations (Na+, Cl, Ca2+) were measured, along with six plant growth indicators. The results showed that increasing AC concentrations significantly increased pH and reduced EC and ion accumulation, with the 5% AC treatment being optimal in both deicer systems. Plant physiological responses were improved in AC-amended soils, especially under CaCl2 treatment, indicating less ion toxicity and better root zone conditions. The interaction effects between AC, deicer type, and plant presence were statistically significant (p < 0.05), supporting a synergistic remediation mechanism involving both adsorption and biological uptake. Despite the limitations of short-term controlled conditions, this study offers a promising phytomanagement strategy using natural adsorbents and salt-tolerant plants for sustainable remediation of salt-affected soils in road-adjacent and urban environments. Full article
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8 pages, 961 KiB  
Proceeding Paper
Analyzing Small-Particle Contamination in Disposable Food Service Ware, Drinking Water, and Commercial Table Salt in Doha, Qatar
by Marwa Al-Ani, Ala Al-Ardah, Mennatalla Kuna, Zainab Smati, Asma Mohamed, Mostafa Sliem and Noora Al-Qahtani
Mater. Proc. 2025, 22(1), 5; https://doi.org/10.3390/materproc2025022005 - 18 Jul 2025
Viewed by 128
Abstract
Microplastics (MPs) have emerged as pervasive environmental contaminants due to their widespread presence across various ecosystems, including their use in single-use plastic food ware and table salt dispensers. This issue coincides with the presence of heavy metals in water sources in Doha, Qatar. [...] Read more.
Microplastics (MPs) have emerged as pervasive environmental contaminants due to their widespread presence across various ecosystems, including their use in single-use plastic food ware and table salt dispensers. This issue coincides with the presence of heavy metals in water sources in Doha, Qatar. Fourier Transform Infrared (FTIR) analysis revealed that the plastic plate and spoon were composed of polyolefin, with the spoon exhibiting additional peaks that indicated oxidation or the presence of additives. Thermogravimetric Analysis (TGA) revealed that the spoon exhibited higher thermal stability, retaining approximately 10% of its mass at 700 °C, than the plate, which retained 2%, indicating the presence of complex additives or contamination. MPs in food-grade salt samples were verified through filtration and Fourier Transform Infrared (FTIR) Spectroscopy, identifying polymers such as polyethylene (PE), polypropylene (PP), and polyethylene terephthalate (PET). These MPs likely stem from exposure to packaging or environmental contaminants. FTIR spectra confirmed the integrity of the polymers after treatment. Inductively Coupled Plasma–Optical Emission Spectroscopy (ICP-OES) analysis revealed varying levels of heavy metals in bottled and tap water, with notable findings including detectable arsenic and lead in both, higher calcium and magnesium in bottled water, and the presence of copper present in tap water only, highlighting potential health and infrastructure-related concerns. These results highlight the possible risks associated with exposure to MPs and heavy metals from everyday products and water sources, underscoring the need for enhanced regulatory oversight and safer material choices to ensure protection. Full article
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23 pages, 3086 KiB  
Article
Comprehensive Analysis of Soil Physicochemical Properties and Optimization Strategies for “Yantai Fuji 3” Apple Orchards
by Zhantian Zhang, Zhihan Zhang, Zhaobo Fan, Weifeng Leng, Tianjing Yang, Jie Yao, Haining Chen and Baoyou Liu
Agriculture 2025, 15(14), 1520; https://doi.org/10.3390/agriculture15141520 - 14 Jul 2025
Viewed by 341
Abstract
Based on an integrated analysis, this study summarized the current status of soil quality in Yantai apple orchards, developed a multivariate regulation model for key soil physicochemical properties, and proposed optimized fertilization strategies to improve soil quality in the region. The study analyzed [...] Read more.
Based on an integrated analysis, this study summarized the current status of soil quality in Yantai apple orchards, developed a multivariate regulation model for key soil physicochemical properties, and proposed optimized fertilization strategies to improve soil quality in the region. The study analyzed the physicochemical properties of the topsoil (0–30 cm) in 19 representative apple orchards across Yantai, including indicators like pH, organic matter (OM), major nutrient ions, and salinity indicators, using standardized measurements and multivariate statistical methods, including descriptive statistics analysis, frequency distribution analysis, canonical correlation analysis, stepwise regression equation analysis, and regression fit model analysis. The results demonstrated that in apple orchards across the Yantai region, reductions in pH were significantly mitigated under the combined increased OM and exchangeable calcium (Ca). Exchangeable potassium (EK) rose in response to the joint elevation of OM and available nitrogen (AN), and AN was also positively influenced by EK, while OM also exhibited a promotive effect on Olsen phosphorus (OP). Furthermore, Ca increased with higher pH. AN and EK jointly contributed to the increases in electrical conductivity (EC) and chloride ions (Cl), while elevated exchangeable sodium (Na) and soluble salts (SS) were primarily driven by EK. Accordingly, enhancing organic and calcium source fertilizers is recommended to boost OM and Ca levels, reduce acidification, and maintain EC within optimal limits. By primarily reducing potassium’s application, followed by nitrogen and phosphorus source fertilizers, the supply of macronutrients can be optimized, and the accumulation of Na, Cl, and SS can be controlled. Collectively, the combined analysis of soil quality status and the multivariate regulation model clarified the optimized fertilization strategies, thereby establishing a solid theoretical and practical foundation for recognizing the necessity of soil testing and formula fertilization, the urgency of improving soil quality, and the scientific rationale for nutrient input management in Yantai apple orchards. Full article
(This article belongs to the Section Agricultural Soils)
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15 pages, 2179 KiB  
Article
High-Salt Exposure Disrupts Cardiovascular Development in Zebrafish Embryos, Brachyodanio rerio, via Calcium and MAPK Signaling Pathways
by Ebony Thompson, Justin Hensley and Renfang Song Taylor
J 2025, 8(3), 26; https://doi.org/10.3390/j8030026 (registering DOI) - 14 Jul 2025
Viewed by 285
Abstract
Cardiovascular disease and hypertension are major global health challenges, and increasing dietary salt intake is a known contributor. Emerging evidence suggests that excessive salt exposure during pregnancy may impact fetal development, yet its effects on early embryogenesis remain poorly understood. In this study, [...] Read more.
Cardiovascular disease and hypertension are major global health challenges, and increasing dietary salt intake is a known contributor. Emerging evidence suggests that excessive salt exposure during pregnancy may impact fetal development, yet its effects on early embryogenesis remain poorly understood. In this study, we used zebrafish (Danio rerio) embryos as a model to investigate the developmental and molecular consequences of high-salt exposure during early vertebrate development. Embryos subjected to elevated salt levels exhibited delayed hatching, reduced heart rates, and significant alterations in gene expression profiles. Transcriptomic analysis revealed over 4000 differentially expressed genes, with key disruptions identified in calcium signaling, MAPK signaling, cardiac muscle development, and vascular smooth muscle contraction pathways. These findings indicate that early salt exposure can perturb crucial developmental processes and signaling networks, offering insights into how prenatal environmental factors may contribute to long-term cardiovascular risk. Full article
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17 pages, 2428 KiB  
Article
Combining Diluted Seawater and Fertilizer in an Ion-Based Multivariate Approach as an Effective Assay of Salt Tolerance in Brassica juncea Seedlings
by Morgan Tomlin, William Bridges, Qiong Su, Raghupathy Karthikeyan, Byoung Ryong Jeong, Haibo Liu, Gary L. Amy and Jeffrey Adelberg
Horticulturae 2025, 11(7), 820; https://doi.org/10.3390/horticulturae11070820 - 10 Jul 2025
Viewed by 315
Abstract
Non-conventional water sources (saline and brackish water) are viable options for crop cultivation. Current salt-tolerance research largely focuses on Na+ and Cl, while other ions in these waters remain ill-understood. Synthetic seawater was a representative of saline and brackish water [...] Read more.
Non-conventional water sources (saline and brackish water) are viable options for crop cultivation. Current salt-tolerance research largely focuses on Na+ and Cl, while other ions in these waters remain ill-understood. Synthetic seawater was a representative of saline and brackish water in a Design of Experiments (DoE) treatment design used to evaluate the effects of factors [synthetic seawater (0, 15, 30, or 45%, v/v, Instant Ocean®), total inorganic nitrogen (0, 14, or 28 mM; 1 NH4+:8 NO3 ratio), potassium (0, 9, or 21 mM), calcium (0, 2, or 5 mM), silicon (0, 0.03, or 0.09 mM) and zinc (0, 0.05, or 2 mM)] on seedlings for two varieties of Brassica juncea [‘Carolina Broadleaf’ (CB) and ‘Florida Broadleaf’ (FB)] using a hydroponic assay. In 30–45% synthetic seawater, 0.09 mM of silicon or 2 mM of calcium alleviated salt stress. In FB, 0.04–0.06 mM of silicon was optimal for the production of new leaves. The CB variety showed greater production of new leaves with 0.09 mM of silicon and 28 mM of potassium. Potassium and calcium are components of seawater, and a sodium chloride assay would not account for their interactions without a multivariate approach to evaluate salt tolerance. The seedling assay identified factors and established criteria for larger-scale harvest experiments. Full article
(This article belongs to the Topic Plants Nutrients, 2nd Volume)
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15 pages, 2397 KiB  
Article
A Double Closed-Loop Process for Nanoparticle Synthesis via Aerosol Mixing and Venturi Jet Scrubbing
by Bruno Fabiano, Marco Salerno, Marco Vocciante, Omar Soda and Andrea Pietro Reverberi
Appl. Sci. 2025, 15(14), 7693; https://doi.org/10.3390/app15147693 - 9 Jul 2025
Viewed by 281
Abstract
Inorganic nanoparticles (NPs) have been synthesised via mixing and coalescence of droplets containing precursors and entrained by gaseous streams. The droplets have been generated by ultrasonic aerosolisation of two different liquid phases, each containing the respective reagent. The as-produced NPs are trapped by [...] Read more.
Inorganic nanoparticles (NPs) have been synthesised via mixing and coalescence of droplets containing precursors and entrained by gaseous streams. The droplets have been generated by ultrasonic aerosolisation of two different liquid phases, each containing the respective reagent. The as-produced NPs are trapped by mixing with a liquid phase in a Venturi nozzle, acting simultaneously as a collector and concentrator of the solid nanosized phase produced. Commercial electrically powered ultrasonic aerosolising devices have been adapted to atomise salt solutions characterised by high electrical conductivity. This process allowed the synthesis of calcium carbonate NPs with an average diameter in the range of (34–52) nm, according to the concentration of precursors in the aerosolised phases. This closed-loop method of synthesis, where neither capping agents were used nor demanding operating conditions were adopted, can represent a safe and viable eco-friendly technique for NP production free of undesirable compounds, as required for pharmaceutical preparations and theranostic uses. Full article
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8 pages, 2004 KiB  
Article
Updating the Mechanism of Bicarbonate (HCO3) Activation of Soluble Adenylyl Cyclase (sAC)
by Jacob Ferreira, Hayden Belliveau, Clemens Steegborn, Jochen Buck and Lonny R. Levin
Int. J. Mol. Sci. 2025, 26(13), 6401; https://doi.org/10.3390/ijms26136401 - 3 Jul 2025
Viewed by 268
Abstract
Soluble adenylyl cyclase (sAC) is molecularly and biochemically distinct from other mammalian nucleotidyl cyclases. It is uniquely regulated directly by bicarbonate (HCO3) and calcium (Ca2+) ions and is responsive to physiologic fluctuations in levels of its substrate, adenosine [...] Read more.
Soluble adenylyl cyclase (sAC) is molecularly and biochemically distinct from other mammalian nucleotidyl cyclases. It is uniquely regulated directly by bicarbonate (HCO3) and calcium (Ca2+) ions and is responsive to physiologic fluctuations in levels of its substrate, adenosine triphosphate (ATP). Our initial in vitro biochemical studies suggested two mechanisms for HCO3-dependent elevation of sAC activity: increasing catalytic rate and relieving inhibition observed in the presence of supraphysiological levels of substrate, ATP. Structural and mutational studies revealed that HCO3 increases catalytic rate via the disruption of a salt bridge that facilitates productive interactions with the substrate. Here, we demonstrate that the HCO3 stimulation observed under supraphysiological ATP concentrations is due to the mitigation of ATP-dependent acidification. Therefore, we conclude that the sole physiologically relevant mechanism of HCO3 regulation of sAC is through its pH-independent effect facilitating productive substrate binding to the catalytic site. Full article
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19 pages, 865 KiB  
Article
Salinity Stress and Calcium in Pomegranate: Impacts on Growth, Ion Homeostasis, and Photosynthesis
by Christos Chatzissavvidis, Nina Devetzi, Chrysovalantou Antonopoulou, Ioannis E. Papadakis, Ioannis Therios and Stefanos Koundouras
Horticulturae 2025, 11(7), 786; https://doi.org/10.3390/horticulturae11070786 - 3 Jul 2025
Viewed by 409
Abstract
Salinity has significant impacts on crops, a problem that is exacerbated under climate change conditions. For this reason, research is focused on possible ways to mitigate the impacts by adapting cultivation methods such as administering appropriate materials or formulations to plants. Therefore, this [...] Read more.
Salinity has significant impacts on crops, a problem that is exacerbated under climate change conditions. For this reason, research is focused on possible ways to mitigate the impacts by adapting cultivation methods such as administering appropriate materials or formulations to plants. Therefore, this study investigated the effects of calcium (Ca2+) supplementation on the growth, physiology, and chemical composition of pomegranate plants (Punica granatum L. cv. ‘Wonderful’) grown under salinity stress. Young self-rooted plants were cultivated in pots containing a sand/perlite (1:1) mixture and irrigated with Hoagland’s nutrient solution amended with NaCl (0, 60, or 120 mM) and CaCl2·2H2O (0 or 10 mM). Salinity significantly reduced the fresh and dry weight of aboveground tissues; photosynthetic performance; chlorophyll content; and potassium (K), calcium (Ca), and magnesium (Mg) concentrations, particularly under high NaCl levels. Sodium (Na) accumulation increased in all plant parts, while nitrogen (N), manganese (Mn), and zinc (Zn) concentrations were elevated in basal leaves. Calcium supplementation mitigated several of these adverse effects, especially under moderate salinity. It helped maintain leaf biomass, supported K+ retention in roots, partially improved chlorophyll concentration, and limited Na+ accumulation in certain tissues. However, Ca2+ application did not consistently reverse the negative impacts of severe salinity (120 mM NaCl), and in some cases, interactions between Ca2+ and other nutrients such as Mg2+ were antagonistic. These findings confirm the inherent salt tolerance of pomegranate and demonstrate that calcium plays a partially protective role under salinity, particularly at moderate stress levels. Further research is needed to optimize Ca2+ use in saline agriculture and enhance sustainable cultivation of pomegranate in salt-affected soils. Full article
(This article belongs to the Special Issue Orchard Management: Strategies for Yield and Quality)
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28 pages, 3292 KiB  
Article
Optimization of the Quality of Reclaimed Water from Urban Wastewater Treatment in Arid Region: A Zero Liquid Discharge Pilot Study Using Membrane and Thermal Technologies
by Maria Avramidi, Constantinos Loizou, Maria Kyriazi, Dimitris Malamis, Katerina Kalli, Angelos Hadjicharalambous and Constantina Kollia
Membranes 2025, 15(7), 199; https://doi.org/10.3390/membranes15070199 - 1 Jul 2025
Viewed by 780
Abstract
With water availability being one of the world’s major challenges, this study aims to propose a Zero Liquid Discharge (ZLD) system for treating saline effluents from an urban wastewater treatment plant (UWWTP), thereby supplementing into the existing water cycle. The system, which employs [...] Read more.
With water availability being one of the world’s major challenges, this study aims to propose a Zero Liquid Discharge (ZLD) system for treating saline effluents from an urban wastewater treatment plant (UWWTP), thereby supplementing into the existing water cycle. The system, which employs membrane (nanofiltration and reverse osmosis) and thermal technologies (multi-effect distillation evaporator and vacuum crystallizer), has been installed and operated in Cyprus at Larnaca’s WWTP, for the desalination of the tertiary treated water, producing high-quality reclaimed water. The nanofiltration (NF) unit at the plant operated with an inflow concentration ranging from 2500 to 3000 ppm. The performance of the installed NF90-4040 membranes was evaluated based on permeability and flux. Among two NF operation series, the second—operating at 75–85% recovery and 2500 mg/L TDS—showed improved membrane performance, with stable permeability (7.32 × 10−10 to 7.77 × 10−10 m·s−1·Pa−1) and flux (6.34 × 10−4 to 6.67 × 10−4 m/s). The optimal NF operating rate was 75% recovery, which achieved high divalent ion rejection (more than 99.5%). The reverse osmosis (RO) unit operated in a two-pass configuration, achieving water recoveries of 90–94% in the first pass and 76–84% in the second. This setup resulted in high rejection rates of approximately 99.99% for all major ions (Cl, Na+, Ca2+, and Mg2+), reducing the permeate total dissolved solids (TDS) to below 35 mg/L. The installed multi-effect distillation (MED) unit operated under vacuum and under various inflow and steady-state conditions, achieving over 60% water recovery and producing high-quality distillate water (TDS < 12 mg/L). The vacuum crystallizer (VC) further concentrated the MED concentrate stream (MEDC) and the NF concentrate stream (NFC) flows, resulting in distilled water and recovered salts. The MEDC process produced salts with a purity of up to 81% NaCl., while the NFC stream produced mixed salts containing approximately 46% calcium salts (mainly as sulfates and chlorides), 13% magnesium salts (mainly as sulfates and chlorides), and 38% sodium salts. Overall, the ZLD system consumed 12 kWh/m3, with thermal units accounting for around 86% of this usage. The RO unit proved to be the most energy-efficient component, contributing 71% of the total water recovery. Full article
(This article belongs to the Special Issue Applications of Membrane Distillation in Water Treatment and Reuse)
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17 pages, 7582 KiB  
Article
Effects of Feeding Calcium Salts from a Mixture of Linseed and Fish Oil on Productive Response, Metabolic Status, and Reproductive Parameters in Early-Lactation Dairy Cows
by Pablo M. Roskopf, Alejandra Cuatrin, Matías Stangaferro, Gino Storani, Emmanuel Angeli, Gustavo J. Hein and Eloy E. Salado
Dairy 2025, 6(4), 34; https://doi.org/10.3390/dairy6040034 - 1 Jul 2025
Viewed by 535
Abstract
This study evaluated the effects of supplementing early-lactation Holstein cows with rumen-protected omega-3 fatty acids (calcium salts) on productive and reproductive performance. Thirty-six multiparous cows were randomly assigned to one of two treatments from 21 ± 2 days before calving to 105 ± [...] Read more.
This study evaluated the effects of supplementing early-lactation Holstein cows with rumen-protected omega-3 fatty acids (calcium salts) on productive and reproductive performance. Thirty-six multiparous cows were randomly assigned to one of two treatments from 21 ± 2 days before calving to 105 ± 5 days in milk (DIM): a Control group (C) or an Omega-3-supplemented group (O-3), receiving a blend of linseed and fish oil (60:40). Both groups were fed isoenergetic diets, with ground corn as the control supplement. Total dry matter and net energy intake did not differ between treatments. A treatment-by-time interaction was observed for milk yield, with cows in the O-3 group producing more milk than controls at specific time points. Additionally, O-3 cows had higher overall protein yield and improved feed efficiency. No differences were found in body weight, BCS, glucose, insulin, IGF-1, or urea concentrations, but a tendency toward higher plasma NEFA and BHBA concentrations and lower energy balance was observed in the O-3 group. Supplementation increased plasma cholesterol and progesterone concentrations and was associated with a higher proportion of cows being pregnant at 130 DIM. These findings suggest that omega-3 supplementation may improve specific aspects of lactational performance and reproductive efficiency without compromising metabolic status. Full article
(This article belongs to the Section Dairy Animal Nutrition and Welfare)
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21 pages, 2873 KiB  
Article
Adaptive Evolution of Sporosarcina pasteurii Enhances Saline–Alkali Resistance for High-Performance Concrete Crack Repair via MICP
by Jieyu Liu, Huaihua Xu, Min Dong, Zilin Cheng, Chenkai Mi, Shuai Sun, Ruiying Zhu and Peipei Han
Microorganisms 2025, 13(7), 1526; https://doi.org/10.3390/microorganisms13071526 - 30 Jun 2025
Viewed by 470
Abstract
Microbially induced calcium carbonate precipitation (MICP) has emerged as a research focus in concrete crack remediation due to its environmental compatibility and efficient mineralization capacity. The hypersaline conditions of seawater (average 35 g/L NaCl) and alkaline environments (pH 12) within concrete cracks pose [...] Read more.
Microbially induced calcium carbonate precipitation (MICP) has emerged as a research focus in concrete crack remediation due to its environmental compatibility and efficient mineralization capacity. The hypersaline conditions of seawater (average 35 g/L NaCl) and alkaline environments (pH 12) within concrete cracks pose significant challenges to the survival of mineralization-capable microorganisms. To enhance microbial tolerance under these extreme conditions, this study employed a laboratory adaptive evolution strategy to successfully develop a Sporosarcina pasteurii strain demonstrating tolerance to 35 g/L NaCl and pH 12. Comparative analysis of growth characteristics (OD600), pH variation, urease activity, and specific urease activity revealed that the evolved strain maintained growth kinetics under harsh conditions comparable to the parental strain under normal conditions. Subsequent evaluations demonstrated the evolved strain’s superior salt–alkali tolerance through enhanced enzymatic activity, precipitation yield, particle size distribution, crystal morphology, and microstructure characterization under various saline–alkaline conditions. Whole-genome sequencing identified five non-synonymous mutated genes associated with ribosomal stability, transmembrane transport, and osmoprotectant synthesis. Transcriptomic profiling revealed 1082 deferentially expressed genes (543 upregulated, 539 downregulated), predominantly involved in ribosomal biogenesis, porphyrin metabolism, oxidative phosphorylation, tricarboxylic acid (TCA) cycle, and amino acid metabolism. In concrete remediation experiments, the evolved strain achieved superior performance with 89.3% compressive strength recovery and 48% reduction in water absorption rate. This study elucidates the molecular mechanisms underlying S. pasteurii’s salt–alkali tolerance and validates its potential application in the remediation of marine engineering. Full article
(This article belongs to the Section Microbial Biotechnology)
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14 pages, 2179 KiB  
Article
One-Pot Anodic Electrodeposition of Dual-Cation-Crosslinked Sodium Alginate/Carboxymethyl Chitosan Interpenetrating Hydrogel with Vessel-Mimetic Heterostructures
by Xuli Li, Yuqing Qu, Yong Zhang, Pei Chen, Siyu Ding, Miaomiao Nie, Kun Yan and Shefeng Li
J. Funct. Biomater. 2025, 16(7), 235; https://doi.org/10.3390/jfb16070235 - 26 Jun 2025
Viewed by 669
Abstract
This study develops a one-pot anodic templating electrodeposition strategy using dual-cation-crosslinking and interpenetrating networks, coupled with pulsed electrical signals, to fabricate a vessel-mimetic multilayered tubular hydrogel. Typically, the anodic electrodeposition is performed in a mixture of sodium alginate (SA) and carboxymethyl chitosan (CMC), [...] Read more.
This study develops a one-pot anodic templating electrodeposition strategy using dual-cation-crosslinking and interpenetrating networks, coupled with pulsed electrical signals, to fabricate a vessel-mimetic multilayered tubular hydrogel. Typically, the anodic electrodeposition is performed in a mixture of sodium alginate (SA) and carboxymethyl chitosan (CMC), with the ethylenediaminetetraacetic acid calcium disodium salt hydrate (EDTA·Na2Ca) incorporated to provide a secondary ionic crosslinker (i.e., Ca2+) and modulate the cascade reaction diffusion process. The copper wire electrodes serve as templates for electrochemical oxidation and enable a copper ion (i.e., Cu2+)-induced tubular hydrogel coating formation, while pulsed electric fields regulate layer-by-layer deposition. The dual-cation-crosslinked interpenetrating hydrogels (CMC/SA-Cu/Ca) exhibit rapid growth rates and tailored mechanical strength, along with excellent antibacterial performance. By integrating the unique pulsed electro-fabrication with biomimetic self-assembly, this study addresses challenges in vessel-mimicking structural complexity and mechanical compatibility. The approach enables scalable production of customizable multilayered hydrogels for artificial vessel grafts, smart wound dressings, and bioengineered organ interfaces, demonstrating broad biomedical potential. Full article
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16 pages, 5674 KiB  
Article
Stage-Dependent Mineral Element Dynamics in ‘Junzao’ Jujube: Ionic Homeostasis and Selective Transport Under Graduated Saline-Alkali Stress
by Ze Yuan, Xiaofeng Zhou, Yuyang Zhang, Yan Wang, Haoyu Yan, Wu Sun, Min Yan and Cuiyun Wu
Horticulturae 2025, 11(7), 726; https://doi.org/10.3390/horticulturae11070726 - 22 Jun 2025
Viewed by 387
Abstract
Plants dynamically regulate ions in the tree to defend against abiotic stresses such as drought and saline-alkali, However, it is not clear how ‘Junzao’ jujube regulates ions to maintain a normal life cycle under saline-alkali stress. Therefore, in this study, the roots of [...] Read more.
Plants dynamically regulate ions in the tree to defend against abiotic stresses such as drought and saline-alkali, However, it is not clear how ‘Junzao’ jujube regulates ions to maintain a normal life cycle under saline-alkali stress. Therefore, in this study, the roots of 10-year old steer jujube trees were watered using a saline and alkaline gradient solution simulating the main salt (NaCl) and alkali (NaHCO3) of Aral with NaCl:NaHCO3 = 3:1 gradient of 0, 60, 180, and 300 mM, and three jujube trees with uniform growth were taken as samples in each treatment plot, and the ion contents of potassium (K), sodium (Na), calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), zinc (Zn) and carbon (C) in each organ of the fruit at the dot red period (S1) and full-red period (S2) were determined, in order to elucidate the relationship between physiological adaptation mechanisms of saline-alkali tolerance and the characteristics of mineral nutrient uptake and utilisation in jujube fruit. The results showed that under saline-alkali stress, Na was stored in large quantities in the roots, Ca and Mg in the perennial branches at S1, Na and Fe in the leaves at S2, and K, Mg and Mn in the perennial branches. There was no significant difference in the distribution of C content in various organs of ‘Junzao’. Compared with CK (0 mM), under salinity stress, the K content in the leaves was significantly reduced at S1 and S2, and the K/Na ratios remained > 1.0. At S2, under medium and high concentrations of saline-alkali stress (180–300 mM), the K/Na is less than 1, and the ionic homeostasis was disrupted, and the leaves die and fall off, and the Na is excreted from the body. The selective transport coefficients SK/Na, SCa/Na and SMg/Na from root to leaf showed a downward trend at S1, but still maintained positive transport capacity. At S2, this stage is close to leaf fall, the nutrient transport coefficient is less than 1, and a large amount of nutrients are returned to the perennial branches and roots occurred. These results indicated that the mechanism of nutrient regulation and salt tolerance in jujube trees was different at different growth stages. Full article
(This article belongs to the Section Biotic and Abiotic Stress)
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16 pages, 390 KiB  
Article
Impact of Balkan and Banat Donkey Milk on the Technological Process, Microbiological Quality, Composition, and Consumer Acceptability of Rolled Cheese
by Suzana Vidaković Knežević, Jelena Vranešević, Nenad Popov, Slobodan Knežević, Dragana Ljubojević Pelić and Milica Živkov Baloš
Foods 2025, 14(12), 2041; https://doi.org/10.3390/foods14122041 - 10 Jun 2025
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Abstract
Donkey milk is well known for its beneficial properties for human health, making it a valuable ingredient in the production of value-added cheese. Rolled cheese, a type of pasta filata cheese, is traditionally produced in the northern part of Serbia. In this study, [...] Read more.
Donkey milk is well known for its beneficial properties for human health, making it a valuable ingredient in the production of value-added cheese. Rolled cheese, a type of pasta filata cheese, is traditionally produced in the northern part of Serbia. In this study, we produced rolled cheese by adding a certain amount of donkey’s milk from the Balkan and Banat breeds to cow’s milk. The rolled cheese samples were analyzed for their microbiological quality, chemical composition, content of essential and trace elements, as well as sensory characteristics. Adding 10% and 20% donkey’s milk had no effect on the microbiological quality or hedonic scale of rolled cheeses compared with rolled cheese made from raw cow’s milk. However, the addition of donkey’s milk partially affected the chemical composition and mineral profile of the cheeses. The fat, fat in dry matter, calcium contents, and the ratio of calcium and phosphorus significantly (p < 0.05) decreased with the addition of donkey’s milk, while the ash, salt, sodium, and potassium contents significantly (p < 0.05) increased. The assessors successfully distinguished the rolled cheeses containing donkey’s milk from those made with cow’s milk, encouraging the future production of value-added cheese. Full article
(This article belongs to the Section Dairy)
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