Search Results (71,775)

Assessing genotoxicity, specifically gene mutations and chromosomal aberrations, is fundamental to chemical risk assessment. Notably, the early identification of an aneugenic mechanism is of crucial importance, allowing, in principle, for a threshold-based risk assessment approach. To investigate this issue while pushing towards innovation in risk assessment by leveraging New Approach Methodologies, in silico approaches stand out as a particularly promising avenue. Building on these premises and given the lack of QSAR models for aneuploidy in the public domain, the present study exploited the genotoxicity-relevant alert lists implemented in the OECD QSAR Toolbox to base the investigation of structure-activity relationships for aneuploidy. To address the lack of relevant structured data resources, a dataset of 65 confirmed aneugenic substances was specifically curated and designed for the study. The results highlighted widely differing performances among the various profilers, confirming a general limited discriminatory power for aneuploidy. On the other hand, a granular analysis of the results from individual structural alerts enabled the successful isolation of some features associated with the aneugenic mode of action. Moreover, a subset of tubulin-binding chemicals was investigated to determine whether targeting a specific protein improves the characterization of toxicological alerts. The findings provide a refined definition of specific toxicity determinants for tubulin binders and serve as a promising tool for early hazard assessment, potentially informing relevant AOPs. While the computational approach appears promising, the overarching challenge that emerges is the limited availability of well-curated experimental data. In fact, reliable data on aneuploidy are scarce and fragmented across the literature. Furthermore, existing compilations of micronucleus study results are often complicated by conflicting interpretations. Full article

The classic design of the Practical Byzantine Fault Tolerance (PBFT) protocol relies on a centralized primary node, which not only creates a performance bottleneck but also introduces severe data censorship risks, threatening the data integrity and security of Edge Computing networks. To address this challenge, this paper proposes DC-PBFT (Decoupled PBFT), a censorship-resistant consensus protocol for Edge-Internet of Things (Edge-IoT) environments. The core innovation of DC-PBFT lies in the decoupling of the Proposer and Primary roles, supplemented by Verifiable Random Function (VRF)-based dynamic role rotation, which fundamentally eliminates the arbitrary power of a single node. Building on this, the protocol introduces a parallel group consensus mechanism: an elected Consensus Committee (CC) composed of Active Edge Nodes leads the consensus, while an independent Replica Network (RN) performs parallel validation. When a disagreement arises, the protocol triggers a global disagreement arbitration process involving all nodes to guarantee final consistency and attribute fault. To ensure long-term incentive compatibility, we also designed a hybrid election mechanism combining Proof-of-Stake and dynamic reputation, along with corresponding economic incentives and a tiered penalty system. Theoretical analysis proves that DC-PBFT satisfies Consistency and Liveness, and achieves strong censorship resistance guarantees. Simulation results demonstrate that DC-PBFT’s scalability significantly outperforms PBFT and RepChain; its reputation mechanism effectively improves long-term performance under sustained Byzantine attacks; and, compared to asynchronous censorship-resistant protocols like HoneyBadgerBFT, DC-PBFT achieves censorship resistance with over 45% lower transaction confirmation latency. Full article

WiFi fingerprinting remains attractive for indoor localization because it reuses existing wireless infrastructure, yet RSSI fingerprints are high-dimensional, sparse, and often ambiguous across adjacent floors and building regions. This study develops a hybrid blended localization framework that combines multi-task learning with feature-space weighted k-nearest-neighbor refinement. A shared neural encoder predicts building labels, floor labels, and normalized coordinates from 520-dimensional WiFi fingerprints, and the learned embedding space is then used for semantically constrained WKNN correction. The final model is trained with AdamW, a learning rate of $8\times {10}^{-4}$, batch size 512, and a joint loss over building classification, floor classification, and coordinate regression, without a learning-rate scheduler. Experiments on a public WiFi fingerprint dataset show that the hybrid model achieves the strongest overall localization robustness among the evaluated non-ensemble methods. On the official validation split, it obtains a mean localization error of 9.01, a median error of 6.25, and an RMSE of 12.95 in the dataset coordinate units. On the internal semantic validation split, it reaches 94.81% floor classification accuracy and 97.62% building classification accuracy. Floor-wise and building–floor analyses further show that the largest errors are concentrated in a small number of difficult semantic regions, especially the highest floor and sparsely constrained partitions. Full article

Remote sensing change detection (RSCD), a fundamental task in Earth observation, aims to automatically identify land-cover changes (e.g., building construction, vegetation degradation) by comparing multitemporal satellite or aerial images of the same region. With the explosive growth of high-resolution remote sensing data, achieving real-time accurate change detection on edge computing devices (e.g., drone-embedded chips, satellite on-board processors) has become an urgent challenge—existing deep learning methods, despite high accuracy, are hindered by massive parameters and computational costs that preclude deployment on resource-constrained embedded hardware. To address this, we focus on lightweight (i.e., low parameter count and low computational cost) RSCD network design, targeting three critical bottlenecks: blurred boundaries of changed regions, missed detection of small objects, and insufficient computational efficiency. We propose ATCFNet (Adjacent-Temporal Cross Fusion Network), featuring a three-step progressive feature optimization strategy: (1) the Adjacent Feature Aggregation Module (AFAM) enhances shallow geometric details via lateral three-stage fusion to compensate for lightweight backbones; (2) the Temporal Attention Cross Module (TACM) integrates cross-level feature propagation and Convolutional Block Attention Module (CBAM) for collaborative optimization of high-level semantics and low-level details; and (3) the Efficient Guidance Module (EGM) establishes long-range dependencies using shared change priors and lightweight self-attention to suppress internal voids in changed regions. Experiments on three public datasets (LEVIR-CD, HRCUS, SYSU-ChangeDet) demonstrate that ATCFNet achieves state-of-the-art accuracy with merely 3.71 million (M) parameters and 3.0 billion (G) floating-point operations (FLOPs)—F1-scores of 91.46%, 77.05%, and 83.53%, significantly outperforming 18 existing methods in most indicators. Notably, it excels in edge integrity (avoiding jagged blurring at change boundaries) and small-target detection in high-resolution urban scenes. This study provides an efficient and reliable lightweight solution for edge computing scenarios such as real-time drone inspection and satellite on-board intelligent processing. Full article

The transition toward low-impact facility management requires robust evidence that environmental optimization does not compromise hygienic reliability. In the professional cleaning sector, sustainability claims are often based on product substitution rather than on integrated performance validation. This study provides a dual-perspective evaluation comparing a conventional cleaning protocol with the Pfe Green system, an eco-designed approach compliant with the Italian Minimum Environmental Criteria (CAM, D.M. 29 January 2021), implemented in a civil building of Renault Italia S.p.A. in Rome. A combined Life Cycle Assessment (LCA) and microbiological evaluation was conducted under real operational conditions to quantify both climate-related and hygienic outcomes. The LCA, performed in accordance with ISO 14040–44 and ISO 14067 standards, demonstrated that the Green protocol achieved an approximately 30% reduction in Global Warming Potential over a 100-year horizon (GWP₁₀₀), mainly attributable to structural process optimization, including reduced detergent mass, lower laundering temperatures, improved dilution control, and energy-efficient machinery. Microbiological monitoring, conducted according to ISO 14698 and UNI EN ISO 18593, showed that both systems ensured high levels of microbial abatement, with the Green protocol exhibiting slightly higher average reductions in total viable counts (96.1% vs. 94.2%). These findings confirm that lower chemical input and eco-formulated products do not compromise hygienic performance when supported by standardized procedures and microfiber-based mechanical removal. By integrating life-cycle metrics with microbiological validation, this research proposes a replicable assessment model for sustainable cleaning services. The results demonstrate that CAM-aligned green protocols can simultaneously reduce greenhouse gas emissions and maintain hygienic effectiveness, thereby supporting evidence-based sustainable procurement and corporate environmental strategies. The originality of this study lies in the integration of life-cycle environmental assessment with real-world microbiological validation under operational conditions, providing a comprehensive framework for evaluating sustainable cleaning systems beyond product-level substitution. Full article

Compared with other construction operations, high-altitude operations are more dangerous. Falling from a height is the main type of accident in construction. It is important to study the human risk of falling from height to reduce falling accidents. Based on the Human Factors Analysis and Classification System (HFACS) model, a preliminary evaluation index system for fall risk in building construction was established. Through the Delphi method and sensitivity analysis, the initial indicators were screened, the index factors that did not meet the requirements were removed, and the final human risk index evaluation system was determined. The system includes five first-level indicators and 17 s-level indicators of organizational influence, unsafe supervision, preconditions for unsafe behavior, and unsafe behavior. Subsequently, the analytic network process–entropy weight method (ANP-EWM) is used to subjectively and objectively weight the evaluation indicators, and the combined weight is obtained through game theory. The matter–element extension model is constructed to evaluate the human risk of falling from height in construction. Finally, an empirical analysis is carried out with the Y project as a case study. The novelty of this study lies in integrating human-factor analysis with the matter–element extension model for fall risk assessment in construction, while combining ANP, the entropy weight method, and game theory to balance subjective and objective weighting. The proposed model provides a practical tool for evaluating and controlling human risk in high-altitude construction operations. The results show that the correlation degree calculated according to the matter–element extension model is K₄ = 3.5, and the human risk of falling from height in the construction of Y project has generally reached an excellent level. However, the evaluation level of some evaluation indexes is still low, which is consistent with the actual situation of construction enterprises in Y project. This model provides a direction for the study of human risk assessment of falling from different construction heights. Full article

Eye tracking (ET) provides process-level indices of how students sample task-relevant information during core academic activities. In school-age learners (6–18 years) with specific learning disorders (SLDs; dyslexia, dysgraphia, and dyscalculia), ET can complement behavioural assessment by quantifying oculomotor patterns linked to decoding, model–production coordination, and stepwise strategy execution. This narrative review synthesises ET findings in SLD across reading, handwriting/copying, and arithmetic and translates them into an applied framework for school-oriented use. We summarise key metrics and Areas of Interest (AOI)-based analyses, highlight technical and data-quality requirements for valid acquisition in educational settings, and outline compact functional assessment protocols integrated with standard academic and neuropsychological measures. Building on these foundations, we propose six hypothesis-driven gaze-contingent paradigms (H1–H6) as preliminary models for future experimental testing rather than as established interventions, and we map each to its current level of empirical support, specifying primary gaze outcomes and curriculum-relevant behavioural endpoints. We emphasise that eye-movement findings in specific learning disorders are heterogeneous and may vary as a function of age, task demands, and comorbidity. Accordingly, credible training effects require retention and transfer probes under standard, non-contingent display conditions, appropriate controls, and explicit developmental interpretation. Eye tracking is positioned as complementary functional evidence and as a platform for experimentally testable, mechanism-based interventions in school-age specific learning disorders. Full article

Since coming into full operation in 2020, the BeiDou-3 Navigation Satellite System (BDS-3) has provided global users with positioning, navigation and time-synchronization services. Satellite clock bias is a key factor that affects real-time precise point positioning (PPP), precise orbit determination and the optimization of navigation message parameters; high-precision prediction of clock bias is therefore critical for improving the accuracy and reliability of BDS-3. To further enhance the prediction accuracy and stability of satellite clock bias, we propose a hybrid model based on Mamba-LSTM. This combined model leverages the strengths of the Multimodal Adaptive Model Building Algorithm (Mamba) and the Long Short-Term Memory neural network (LSTM) to predict satellite clock bias. Using precise BDS-3 satellite clock bias data from the International GNSS Service (IGS), we carried out prediction experiments. First, we compared the proposed model’s predictive performance with that of the Mamba and LSTM models. In short-term (6 h) and long-term (24 h) prediction scenarios, the average prediction RMSE of Mamba-LSTM improved by approximately 41.7% and 48% relative to Mamba, and by approximately 50.4% and 54.7% relative to the LSTM results, respectively. Next, we ran comparison experiments against traditional neural networks—the BP model and the CNN model. In mid-term (12 h) and long-term (24 h) prediction scenarios, the average prediction RMSE of Mamba-LSTM improved by approximately 59.6% and 63.1% compared with BP, and by approximately 52.4% and 56.2% compared with CNN, respectively. The results indicate that the Mamba-LSTM hybrid model can significantly improve the accuracy and stability of satellite clock bias prediction. Full article

Many place recognition approaches, which identify previously visited places or locations by matching current sensory data, such as 2D RGB images and 3D point clouds, have been proposed to achieve accurate and robust localization and loop closure detection in global positioning system (GPS)-denied environments. Since visual place recognition (VPR) methods that rely on images captured by camera sensors are highly sensitive to variations in appearance, including changes in lighting, surface color, and shadows, they can lead to poor place recognition accuracy. In contrast, light detection and ranging (LiDAR)-based place recognition (LPR) approaches based on 3D point cloud data that captures the shape and geometric structure of the environment are robust to changes in place appearance and can therefore provide more reliable place recognition results than VPR methods. This work presents an indoor LPR method called PointNetVLAD-based indoor pedestrian localization (PIPL). PIPL is a deep network model that uses PointNetVLAD to learn to extract global descriptors from 3D LiDAR point cloud data. PIPL can recognize places previously visited by a pedestrian using point clouds captured by a low-cost LiDAR sensor on a smartphone in small-scale indoor environments, while PointNetVLAD performs place recognition for vehicles using high-cost LiDAR, GPS, and inertial measurement unit (IMU) sensors in large-scale outdoor areas. For place recognition on 3D point cloud reference maps generated from LiDAR scans, PointNetVLAD exploits the universal transverse mercator (UTM) coordinate system based on GPS and IMU measurements, whereas PIPL uses a virtual coordinate system designed in this study due to the unavailability of GPS indoors. In experiments conducted in campus buildings, PIPL shows significant advantages over NetVLAD (known as a convolutional neural network (CNN)-based VPR method). Particularly in indoor environments with repetitive scenes where geometric structures are preserved and image-based appearance features are sparse or unclear, PIPL achieved $39\%$ higher top-1 accuracy and $10\%$ higher top-3 accuracy compared to NetVLAD. Furthermore, PIPL achieved place recognition accuracy comparable to NetVLAD even with a small number of points in a 3D point cloud and outperformed NetVLAD even with a smaller model training dataset. The experimental results also indicate that PIPL requires over $76\%$ less place retrieval time than NetVLAD while maintaining robust place classification performance. Full article

Unlike reactive systems, mechanical ventilation controlled by CO₂ concentration operates at a target efficiency that dynamically increases whenever the target CO₂ level is exceeded. This approach eliminates the typical ‘dead-time’ and prevents air quality degradation by ensuring the system adjusts its performance immediately in response to concentration changes. In this work, the study focuses on the development and evaluation of data-driven predictive models for near-term indoor CO₂ forecasting that can be integrated into pre-occupancy ventilation strategies, rather than designing a complete control scheme. Experimental data were collected over four months in a 48 m² smart laboratory configured as an open-plan office, where a heterogeneous IoT sensing architecture logged synchronized time-series measurements of CO₂ and microclimate variables (temperature, relative humidity, PM_2.5, TVOCs), together with acoustic noise levels and appliance-level energy consumption used as indirect occupancy-related signals. Raw telemetry was transformed into a 22-feature state vector using a structured feature engineering method incorporating z-score standardization, cyclic time encodings, multi-horizon CO₂ lags, rolling statistics, momentum features, and non-linear interactions to represent temporal autocorrelation and daily periodicity. The study benchmarks multiple regression paradigms, including simple baselines and ensemble methods, and found that an automated multi-level stacked ensemble achieved the highest predictive fidelity for short-term forecasting, with an Mean Absolute Error (MAE) of 32.97 ppm across an observed CO₂ range of 403–2305 ppm, representing improvements of approximately 24% and 43% over Linear Regression and K-Nearest Neighbors (KNN), respectively. Temporal diagnostics showed strong phase alignment with observed CO₂ rises during occupancy transitions and statistically reliable prediction intervals. Five-fold walk-forward cross-validation confirmed the temporal stability of these results, with top models achieving consistent R² values of 0.93–0.95 across Folds 2–5. These results demonstrate that, within a single-room university laboratory setting, historical sensor data from low-cost IoT devices can support accurate short-term CO₂ forecasting, providing a predictive layer that could support future proactive ventilation scheduling aimed at reducing CO₂ lag at the start of occupancy while avoiding unnecessary ventilation runtime. Generalization to other building types and occupancy profiles requires further validation. Full article

To mitigate local congestion and address the adaptability limitations of traditional static routing under dynamic traffic, this paper proposes an end-to-end routing method based on a Graph Attention Network (GAT), termed Congestion-Aware Graph Attention Routing (CA-GAR). To alleviate the issue of local optima in traditional heuristic iterative optimization, we design a dynamic link cost optimization algorithm with multi-start parallel exploration. This algorithm employs a ”penalty–reselection–reward” closed-loop feedback mechanism, performing global searches from multiple random initial states to generate a high-quality, empirically near-optimal cost matrix as supervised labels. Building on this, CA-GAR leverages a multi-head attention mechanism to adaptively aggregate high-order topological features of nodes and edges, and incorporates a staged hierarchical hyperparameter optimization strategy to map real-time network states to link costs. Simulation results demonstrate that CA-GAR outperforms traditional static routing under light, medium, and heavy loads. Under high-load burst conditions, the method exhibits effective congestion avoidance capability, reducing end-to-end delay by approximately 50% and lowering the packet loss rate to as low as 2%. Compared with QLRA, CA-GAR shows promising performance in multi-path traffic splitting and possesses robust fast rerouting capabilities during node failures, thereby achieving intelligent traffic distribution and global load balancing. Full article

Growing concerns about global climate change and its negative consequences for communities have put immense pressure on the building industry, which is one of the primary sources of greenhouse gas emissions. Due to the environmental issues associated with the manufacture of sustainable construction materials, alkali-activated concrete (AAC) has emerged as a competitive alternative to cement. To predict the compressive strength (CS) of AAC, four machine learning (ML) models, namely, Convolutional Neural Network (CNN), Long Short-Term Memory (LSTM), Random Forest (RF), and Extreme Gradient Boosting (XGBoost), were employed in this study using 193 data points. The input variables include Precursor “P” (kg/m³), Blast Furnace Slag “BFS ratio”, Sodium hydroxide “Na” (kg/m³), silicate modulus “Ms”, water content “W” (kg/m³), fine aggregate “FA” (kg/m³), coarse aggregate “A” (kg/m³), and curing time “CT” (day), with CS (MPa) as the output variable. The dataset was checked for stationarity and then normalized to decrease data redundancy and increase integrity. Furthermore, three model combinations were developed based on the relationship between the input and target variables. The XGB-M3 model outperformed all other models with a high degree of accuracy, according to the study’s findings. Specifically, the Pearson correlation coefficient (PCC) was 0.9577, and the mean absolute percentage error (MAPE) was 14.95% during the calibration phase. SHAP, an explainable AI approach that provides interpretable insights into complex AI systems by assigning feature importance to model predictions, was employed. Results suggest the higher predictions from the XGB-M3 and RF-M3 models were largely driven by curing time (CT). Full article

Industrial surface inspection is fundamental to advanced manufacturing, yet reliable robotic image acquisition in complex geometries remains challenging due to severe occlusions and the inherent trade-off between resolution and coverage. Inspired by human visual inspection behaviors and perception–action coordination mechanisms, this paper proposes a hierarchical trajectory optimization framework for robotic image acquisition based on measured point clouds. Specifically, a multi-constraint preprocessing model is developed to emulate human-like active perception strategies, enabling occlusion-aware viewpoint generation over complex concave and convex surfaces with adaptive camera orientation. Building upon this, a multi-objective trajectory optimization method is introduced to coordinate global coverage and local motion efficiency, jointly optimizing viewpoint sequencing, path length, and motion smoothness hierarchically. To further enhance flexibility in constrained environments, a Pose Reachability Augmented Generative Adversarial Network (PRAGAN) is proposed to learn feasible and adaptable imaging postures under kinematic constraints. Experimental results on an industrial robotic platform equipped with 2D and 3D vision systems demonstrate 100% coverage of key surface areas, a 47.0% reduction in path length, and a 37.5% decrease in solution time compared with the baseline in the physical experiments, while ensuring collision-free operation. Both simulation and real-world experiments validate that the proposed framework effectively captures human-inspired perception and motion coordination, providing a practical and scalable solution for complex industrial surface inspection. Full article

This study addressed the issue of insufficient activity in CALB lipase during the catalytic synthesis of key chiral intermediates for moxifloxacin. A structure-guided protein engineering strategy was employed to systematically modify its functional domains. Through molecular dynamics simulations of CALB-I189K, multiple regions exhibiting high conformational flexibility were preliminarily identified. Subsequently, by integrating 3D structural alignment with active site pocket distance analysis, the functionally most critical region (143–146) was selected. A site-directed saturation mutation library was constructed specifically targeting this region. Building upon the previously reported CALB-I189K, a mutant I189K/L144R/A146K was ultimately obtained through high-throughput screening combined with chiral HPLC validation. This mutant maintains excellent stereoselectivity (E = 206.52) while enhancing catalytic efficiency (k_cat/Κ_m) to 273.73 min⁻¹·mM⁻¹, approximately 4.5-fold that of I189K. At a substrate concentration of 1 M, it achieves 50% conversion within 2.6 h, demonstrating kinetic resolution capabilities approaching industrial standards. Molecular simulation analysis indicates that the L144R and A146K mutations synergistically enhance catalytic performance primarily by optimizing spatial distances between catalytic residues. This study not only provides a high-performance catalyst for the efficient biosynthesis of moxifloxacin chiral intermediates but also offers new insights for enzyme rational design based on dynamic structural information. Full article

This paper describes the thermal and energy performance of three roof configurations: a conventional concrete slab, a BIPV system, and a BIPV system equipped with passive aluminum fins. Three-dimensional transient finite element simulations were carried out under field-measured 24 h meteorological boundary conditions characteristic of hot climates. The objective of this study is to quantify the impact of PV integration and passive cooling strategies on heat transfer behavior and building energy performance. The BIPV roof achieved a 38.4% lower residual temperature than the concrete slab at 19:00, indicating superior heat dissipation. The addition of passive fins reduced module temperature by up to 10–12 °C and decreased peak roof temperature by up to 12%. This temperature reduction decreased electrical losses from 13.2% to 10.4%, resulting in a 21% relative reduction in temperature-induced losses. The predicted temperature ranges (≈60–75 °C under peak conditions) are consistent with values reported in experimental and numerical studies of BIPV systems in hot climates, supporting the physical realism of the model. Convective heat transfer was represented using effective coefficients, providing a computationally efficient engineering approximation of air-side heat exchange. Despite construction cost increases of up to 38%, PV integration achieved competitive payback periods of approximately 8.5–9 months under hot climate conditions. This economic assessment is based on a simple payback approach using an incremental cost formulation, where the photovoltaic system replaces the conventional concrete roof, reducing the effective investment. This study introduces a reproducible 3D transient FEM methodology for evaluating BIPV roofs under field-measured climatic boundary conditions. The framework explicitly couples geometry-resolved passive cooling, full-day thermal evolution, and temperature-dependent electrical losses, providing a physically consistent basis for assessing BIPV design alternatives in hot climates. Full article