Search Results (404)

Currently, ω-3 polyunsaturated fatty acids (PUFAs), which have become popular as dietary supplements, are limited by a shortage in supply. Thus, finding safe, effective alternatives is crucial. Echium seed oil (ESO), rich in α-linolenic acid (ALA, 18:3ω-3) and stearidonic acid (SDA, 18:4ω-3), surpasses many other botanical seed oils. In this study, a pseudotargeted approach was applied to characterize the lipidomic profile of two unexplored Echium species from the Mediterranean region. Our findings established Echium glomeratum as a rich source of ω-3 fatty acids (FAs), exceeding many other species in both quality and quantity. E. glomeratum possesses different FAs and triglyceride (TG) profiles compared to E. judaeum, with the ω-3:ω-6 ratio being 3.5 and 1.3, respectively. This corresponds to higher quantities of ALA (45.50%) and SDA (12.59%) in E. glomeratum. Triglycerides (TGs) comprise 93% of the total lipid content in ESO. This study also profiled the most abundant TGs (50–60 carbons) from both species through comprehensive assignment of the olefination patterns. The E. glomeratum oil profile, containing a higher ω-3 PUFA concentration, was further screened for cytotoxic and antioxidant activities. Our preliminary results demonstrated that E. glomeratum ESO may significantly suppress colon cancer cell growth. Full article

Sensitization to non-specific lipid transfer proteins (nsLTPs) is highly prevalent in Mediterranean countries. Pru p 3 from peach is a major allergen responsible for IgE-mediated food allergies. As a panallergen, Pru p 3 shows high sequence homology with nsLTPs from other Rosaceae fruits but also from botanically unrelated sources, including nuts and pollens, leading to extensive cross-reactivity complicating diagnosis and management. Given the worldwide prevalence of peanut and tree nut allergies, this study aimed to investigate sensitization patterns in Pru p 3-sensitized patients with tree nut allergy, using artificial intelligence (AI) to identify predictors of clinical reactivity and severity. Data from Pru p 3–sensitized patients with symptoms to peach and/or nuts were analyzed. Sensitization profiles were modeled using an XGBoost algorithm to explore associations with symptoms and severity. Patients sensitized to Pru p 3 and symptomatic for peach and nuts showed predominant sensitization to peanut and hazelnut, but AI revealed stronger associations between clinical reactivity and sensitization to hazelnut, walnut, and almond. Among patients with nut allergy and peach-asymptomatic, peanut and hazelnut sensitization were most frequent, while peach-symptomatic ones, walnut and almond sensitization predominated. Overall, walnut sensitization emerged as the main predictor of clinical severity and increasing number of sensitizations correlated with higher severity. The XGBoost algorithm identified specific allergen combinations associated with symptoms and severity, highlighting walnut sensitization as the strongest severity predictor. Machine learning approaches represent a promising tool for refining risk stratification and personalizing management in nsLTP-related food allergy. Full article

This study develops a comprehensive workflow integrating Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry (HS-SPME-GC-MS) with advanced supervised machine learning to authenticate the botanical origin of honeys from five distinct floral sources—coriander, orange blossom, astragalus, rosemary, and chehelgiah. While HS-SPME-GC-MS combined with traditional chemometrics (e.g., PCA, LDA, OPLS-DA) is well-established for honey discrimination, the application and direct comparison of Random Forest (RF), eXtreme Gradient Boosting (XGBoost), and Neural Network (NN) models represent a significant advancement in multiclass prediction accuracy and model robustness. A total of 57 honey samples were analyzed to generate detailed volatile organic compound (VOC) profiles. Key chemotaxonomic markers were identified: anethole in coriander and chehelgiah, thymoquinone in astragalus, p-menth-8-en-1-ol in orange blossom, and dill ester (3,6-dimethyl-2,3,3a,4,5,7a-hexahydrobenzofuran) in rosemary. Principal component analysis (PCA) revealed clear separation across botanical classes (PC1: 49.8%; PC2: 22.6%). Three classification models—RF, XGBoost, and NN—were trained on standardized, stratified data. The NN model achieved the highest accuracy (90.32%), followed by XGBoost (86.69%) and RF (83.47%), with superior per-class F1-scores and near-perfect specificity (>0.95). Confusion matrices confirmed minimal misclassification, particularly in the NN model. This work establishes HS-SPME-GC-MS coupled with deep learning as a rapid, sensitive, and reliable tool for multiclass honey botanical authentication, offering strong potential for real-time quality control, fraud detection, and premium market certification. Full article

Roses held profound cultural and economic significance in ancient Greece and Rome, yet comprehensive documentation of their species diversity, cultivation practices, and horticultural innovations remains fragmented across archaeological, iconographic, and textual sources. This multidisciplinary study synthesizes evidence from classical texts, archaeological remains including recently identified rose stem fragments from Oplontis, and iconographic materials—including frescoes, coins, and mosaics—to reconstruct the horticultural systems and cultural landscape of roses in classical antiquity. Analysis of literary sources, particularly Theophrastus’s fourth-century BCE taxonomic descriptions, reveals systematic cultivation of diverse rose varieties with flowers ranging from white to deep crimson, including yellow variants, characterized by morphologies from simple to double forms and valued for fragrance intensity and re-blooming capacity. Archaeological evidence from sites such as Paestum, Pompeii, and Oplontis, including pollen samples, preserved wood fragments with diagnostic prickle patterns, and fresco representations, documents commercial rose production and specialized cultivation techniques that demonstrate significantly greater morphological diversity than textual sources alone indicate. Field research and collection documentation establish the origins of Mediterranean rose cultivation, while iconographic analysis identifies roses in religious ceremonies, festivals, and daily life contexts. Textual sources provide detailed propagation methods, seasonal management practices, and evidence of Mediterranean hybridization events, alongside extensive documentation of medicinal and cosmetic applications. Economic analysis reveals specialized trade networks, commercial production centers, and diverse applications in perfumery, garland making, and pharmaceutical industries. This research establishes that Greek and Roman civilizations developed sophisticated rose cultivation systems integrating botanical selection, horticultural innovation, and cultural symbolism that directly influenced medieval and Renaissance practices and informed modern trait categorization systems. These findings demonstrate the foundational role of classical antiquity in European rose heritage, revealing how ancient horticultural knowledge, species diversification through hybridization, and cultivation techniques created an unbroken transmission that shaped contemporary rose industries and established conservation priorities for this horticultural heritage. Full article

This study examines consumer behavior, preferences, and knowledge regarding honey in Kosovo to inform more effective production, marketing, and policy strategies. Data were collected from 503 respondents through an online questionnaire and analyzed using a combination of artificial neural networks (ANN), decision tree modeling (CHAID), and ordinal logistic regression. The results show a high prevalence of honey consumption, strong preference for locally produced honey, and significant variability in consumer willingness to pay (WTP) based on knowledge, income, and trusted information sources. ANN identified recommendations and product familiarity as primary predictors of WTP, while the decision tree highlighted knowledge and income as key variables for segmentation. The ordinal logistic regression confirmed the importance of perceived quality and product attributes, particularly botanical and geographical origin, in shaping purchasing decisions. The use of complementary statistical models enhanced both predictive power and interpretability. The findings highlight the crucial role of consumer education and trust cues in fostering sustainable honey markets in Kosovo. Full article

Background and Rationale: Tinospora crispa (L.) Hook.f. & Thomson (T. crispa) is a climbing medicinal plant with long-standing ethnopharmacological use, particularly in inflammatory and hepatic disorders and cancer-related conditions. There is a knowledge gap regarding how wild versus cultivated ecotypes differ in chemotype, bioactivity, and safety, and how this might support or refine traditional use. Study Objectives: This study aimed to compare wild and cultivated ecotypes of T. crispa from the Nile Delta (Egypt) in terms of quantitative and qualitative phytochemical profiles; selected in vitro biological activities (especially antioxidant and cytotoxic actions); genetic markers potentially associated with metabolic variation; and short-term oral safety in an animal model. Core Methodology: Standardized extraction of plant material from wild and cultivated ecotypes. Determination of total phenolics, total flavonoids, and major phytochemical classes (alkaloids, tannins, terpenoids). Metabolomic characterization using UHPLC-ESI-QTOF-MS, supported by NMR, to confirm key compounds such as berberine, palmatine, chlorogenic acid, rutin, and borapetoside C. In vitro bioassays including: Antioxidant activity (e.g., radical-scavenging assay with EC₅₀ determination). Cytotoxicity against human cancer cell lines, with emphasis on HepG2 hepatoma cells and calculation of IC₅₀ values. Targeted genetic analysis to detect single-nucleotide polymorphisms (SNPs) in the gen1 locus that differentiate ecotypes. A 14-day oral toxicity study in rats, assessing liver and kidney function markers and performing histopathology of liver and kidney tissues. Principal Results: The wild ecotype showed a 43–65% increase in total flavonoid and polyphenol content compared with the cultivated ecotype, as well as substantially higher levels of key alkaloids, particularly berberine (around 12.5 ± 0.8 mg/g), along with elevated chlorogenic acid and borapetoside C. UHPLC-MS and NMR analyses confirmed the identity of the main bioactive constituents and defined a distinct chemical fingerprint for the wild chemotype. Bioassays demonstrated stronger antioxidant activity of the wild extract than the cultivated one and selective cytotoxicity of the wild extract against HepG2 cells (IC₅₀ ≈ 85 µg/mL), being clearly more potent than extracts from cultivated plants. Genetic profiling detected a C → T SNP within the gen1 region that differentiates the wild ecotype and may be linked to altered biosynthetic regulation. The 14-day oral toxicity study (up to 600 mg/kg) revealed no evidence of hepatic or renal toxicity, with biochemical markers remaining within physiological limits and normal liver and kidney histology. Conclusions and Future Perspectives: The wild Nile-Delta ecotype of T. crispa appears to be a stress-adapted chemotype characterized by enriched levels of multiple bioactive metabolites, superior in vitro bioactivity, and an encouraging preliminary safety margin. These findings support further evaluation of wild T. crispa as a candidate source for standardized botanical preparations targeting oxidative stress-related and hepatic pathologies, while emphasizing the need for: More comprehensive in vivo efficacy studies. Cultivation strategies that deliberately maintain or mimic beneficial stress conditions to preserve phytochemical richness. Broader geographical and genetic sampling to assess how generalizable the present chemotypic and bioactivity patterns are across the species. Full article

The composition and biological activity of bee pollen are strongly influenced by its botanical origin, which is determined by the regional flora and environmental conditions. In Algeria, despite the growing consumption and traditional use of bee products, especially in the Mediterranean region known for its rich floral biodiversity, scientific studies on bee pollen remain scarce. This study aims to characterize bee pollen from the Mediterranean region of Algeria by identifying its botanical origin and evaluating its total phenolic content, total flavonoid content, antioxidant activity, and minerals. A total of 27 bee pollen samples were collected and subjected to palynological analysis to determine their floral sources. Total phenolic content and total flavonoid content were determined by standard colorimetric assays, and antioxidant activity was assessed using RSA with a DPPH assay, ABTS⁺•, and FRAP methods. The results revealed significant variability in the phenolic composition and antioxidant capacity of the samples, depending on their botanical origin. Pollen types such as Brassica napus type, Acacia, Myrtus communis, and Sinapis alba showed notably higher phenols, flavonoids, and antioxidant activity, whereas Hedysarum and Daucus carota pollen exhibited the lowest values. The mineral profile, including macro- and microelements (such as K, Ca, Mg, P, Fe, Cu, Mn, and Na), was determined to explore the nutritional value and potential correlations with biochemical parameters. These findings highlight the influence of floral biodiversity on the bioactive potential of bee pollen and underscore the value of Algerian bee pollen as a promising source of natural antioxidants. Full article

Valorizing fruit and vegetable residues as renewable sources of bioactive compounds (BCs) is critical for advancing sustainable biotechnology. This review (i) assesses the occurrence, diversity and functionality of BCs in 20 edible plant residues; (ii) compares and classify them by botanical family and residue type; (iii) reviews and evaluates the efficiency of conventional and green extraction and characterization techniques for recovering phytochemical and isolating phenolics (e.g., flavonoids and anthocyanins), carotenoids, alkaloids, saponins, and essential oils; and (iv) examines the BCs’ environmental, medical, and industrial applications. It synthesizes current knowledge on the phytochemical potential of these crops, highlighting their role in diagnostics, biomaterials, and therapeutic platforms. Plant-derived nanomaterials, enzymes, and structural matrices are employed in regenerative medicine and biosensing. Carrot- and pumpkin-based nanoparticles accelerate wound healing through antimicrobial and antioxidant protection. Spinach leaves serve as decellularized scaffolds that mimic vascular and tissue microenvironments. Banana fibers are used in biocompatible composites and sutures, and citrus- and berry-derived polyphenols improve biosensor stability and reduce signal interference. Agro-residue valorization reduces food waste and enables innovations in medical diagnostics, regenerative medicine, and circular bioeconomy, thereby positioning plant-derived BCs as a cornerstone for sustainable biotechnology. The BCs’ concentration in fruit and vegetable residues varies broadly (e.g., total phenolics (~50–300 mg GAE/g DW), anthocyanins (~100–600 mg C3G/g DW), and flavonoids (~20–150 mg QE/g DW)), depending on the crop and extraction method. By linking quantitative food waste hotspots with phytochemical potential, the review highlights priority streams for the circular-bioeconomy interventions and outlines research directions to close current valorization gaps. Full article

The first comprehensive study about of amber from the Lower Cretaceous of the Lusitanian Basin, in the Estremadura region of western Portugal, is here reported. The amber was recovered as isolated clasts in the Portela da Vila outcrop section, near the small villages of Ramalhal and Ameal, from sedimentary deposits belonging to the Lugar d’Além Formation considered to be of Hauterivian age. The chemical composition of amber clasts was examined in order to infer their botanical source via biomarker analysis. GC–MS and GC×GC–TOFMS showed a strong predominance of abietane-type diterpenoids, including compounds such as amberene, ferruginol (phenolic abietane), kaurane and the derivative of clerodane. The dominance of abietane diterpenoids along with these specific biomarkers is consistent with resin exudation by Araucariaceae/Cheirolepidiaceae conifers, as supported by previous chemotaxonomic studies of Cretaceous amber. Palynological studies of the same sedimentary rock samples highlighted a pollen–spore assemblage characterized by low diversity and number of specimens, and dominated by conifer pollen assigned to the genera Classopollis, Araucariacites and coniferous bisaccate pollen, with relative occurrences of fern spores. The combined geochemical and palynological studies strongly support a source related to conifer plants for the amber here reported. Full article

Mycotoxins are toxic secondary metabolites produced mainly by filamentous fungi of the genera Aspergillus, Penicillium, and Fusarium and pose a significant food safety concern. This review summarizes current literature on the occurrence of major regulated and emerging mycotoxins, including aflatoxins, ochratoxin A, fumonisins, trichothecenes, zearalenone, and selected Fusarium and Alternaria metabolites, in herbs, spices, and plant-based dietary supplements. Available data indicate that spices—particularly chilli, paprika, ginger, and various types of pepper—represent high-risk commodities and are often more heavily contaminated than dried herbs. Although reported concentrations of individual mycotoxins are frequently low to moderate, numerous studies highlight the common co-occurrence of multiple toxins within a single product, raising concerns regarding cumulative and combined toxic effects. Dietary supplements, especially those containing concentrated plant extracts such as green tea or green coffee, are also identified as potential sources of multi-mycotoxin exposure. The review outlines key analytical approaches for mycotoxin determination, emphasizing the critical role of sample preparation for chromatographic analysis in complex plant matrices. Despite increasing evidence of contamination, important knowledge gaps persist regarding emerging mycotoxins, underrepresented botanical matrices, and long-term exposure assessment, while regulatory limits remain incomplete or inconsistent. Continued monitoring and harmonized analytical and risk assessment strategies are, therefore, essential to ensure consumer safety. Full article

Background/Objectives. Edible oils derived from herbaceous and woody plants are an important nutritional resource, assuring the health and performance of sportspeople. The aim of this study was to review the inventions and experimental articles referring to the application of vegetable oils in food products for sportspeople and published in the period of 2015–2024. Methods. The literature search was conducted across Google Scholar, Scopus, and ISI Web of Science databases, as well as by using Google Patents and Espacenet Patent search engines. Results. Altogether, 58 patents and 35 original articles were found. In total, the use of 39 plant taxa belonging to 27 botanical families was documented. The majority of disclosures refer to sports nutrition, post-exercise recovery support, and/or sport performance improvement and may be provided in the form of powders, tablets, beverages, and/or capsules. According to the reviewed studies, the consumption of olive, walnut, and perilla oils beneficially affects the morphological, physiological, and biochemical indicators of sportspeople. The substantial intake of olive oil reported by sportspeople from southern Europe is linked to the recommendations of the Mediterranean diet, while lower consumption of other vegetable oils might be connected to focusing on intake of carbohydrates and/or proteins and/or consumption of other fat sources such as seeds or nuts. Conclusions. Considering the great potential of useful plant species, it might be concluded that future investigations should focus on both (i) further investigations of the effects of well-known vegetable oils on the health and performance of sportspeople, and (ii) searching for novel plant oil sources, suitable for the preparation of food products dedicated to amateur and professional sportspeople. Full article

Skin aging, driven by oxidative stress, UV exposure, inflammation, and extracellular matrix degradation, necessitates the discovery of safer, multi-target natural products. We established an integrated pipeline combining UHPLC–MS/MS metabolomics, computational methods (network pharmacology, molecular docking, and dynamics simulation), and in vitro bioassays to efficiently discover and mechanistically characterize anti-aging compounds from novel botanical sources. We applied this pipeline to identify and evaluate Tephroseris flammea, a previously unassessed plant. Metabolomic profiling identified 21 compounds, including flavonoids, phenylpropanoids, and pyrrolizidine alkaloids. These compounds were linked via network pharmacology to 226 skin-aging-related targets, primarily involving inflammation (via AKT1, RELA) and matrix degradation (via MAPK3). Molecular docking and 100 ns molecular dynamics simulations confirmed stable ligand-target interactions with favorable binding energies. Validating these in silico predictions, the T. flammea extract demonstrated significant antioxidant activity and effectively suppressed key inflammatory mediators (IL-6, TNF-α, COX-2) and MMP-1 levels in UVB-exposed fibroblasts, notably without significant cytotoxicity. Collectively, this study validates the utility of our pipeline to mechanistically characterize complex botanicals, revealing that T. flammea contains multifunctional compounds modulating critical inflammatory and matrix-regulatory cascades. This work validates our pipeline for identifying novel, mechanistically defined ingredients from complex botanical sources. Full article

Propolis is an apicultural product known for its antioxidant, antimicrobial and anti-inflammatory properties. However, its composition varies with botanical sources, geography, season and bee species, complicating quality control and creating opportunities for adulteration, such as the addition of poplar bud extracts or non-propolis resins. This review synthesizes the latest primary studies and reviews addressing chemical markers identified through analytical platforms, such as TLC, HPTLC, HPLC, LC-MS, GC-MS, NMR, FTIR and ICP, often integrated with chemometrics and machine learning for authentication and standardization. Marker panels are linked to regional chemotypes, including poplar-type, Brazilian green, red and brown, Cuban variants, and stingless bee propolis. Fraud detection strategies using marker-based screening and spectral pattern recognition are also summarized. Multi-marker and chemometric approaches consistently differentiate botanical types, origins and commercial extracts. Common marker families include flavonoids (pinocembrin, chrysin, galangin), phenolic esters (CAPE, benzyl/allyl caffeates), prenylated cinnamates like artepillin C, lignans, and volatile terpenoids or benzenoids. Rapid screening by ATR-FTIR and NMR is often complemented with LC-MS for confirmatory quantitation. Propolis quality control is moving toward harmonized workflows combining FTIR/NMR/HPTLC screening with LC-MS verification and optional elemental or volatile profiling, paving the way for shared marker sets and international standards similar to those for honey. Full article

Woody plants are integral to the ecological and cultural context of the ancient Near East. Biblical references to trees reflect both their practical uses and their symbolic significance. This is a systematic review focused specifically on botanical affiliation, geographical origin and natural habitat type and the cultivation potential of 97 woody species in temperate urban environments, important to ancient economy, culture and religion and consistently identified by scholars in biblical texts. The study applies a multifaceted methodological framework that integrates i.a. textual analysis, literature review and 20 years of horticultural observations. Moreover, the historical utility of these species was studied based on interpreting Bible quotes and comparative multilingual analysis of biblical texts. Analyzed woody plant species represent 36 botanical families, over 50% native to Ancient Palestine. About 18.6% were cultivated by humans, the rest grew in various habitats. Biblical sources revealed 17 uses, with many species having symbolic, practical, or multiple roles. Further, 32% of the species discussed can be grown directly in the soil in temperate climate, while 52.5% require container cultivation. Additionally, 15.5% of the species are hard to cultivate and thus not recommended for Biblical gardens. The content presented also provides valuable insights that may support the development of Biblical gardens within urban environments worldwide. Full article

Background: Resistant starch, and specially retrograded starches (RS), have been suggested as useful biological molecules to improve the glucose management in diabetic conditions. However, the influence of the botanical origin in the RS biological capacities make necessary its evaluation, where RS from legumes have been paid less attention compared to other sources as cereals. Objectives: A RS product obtained from creole Faba beans (Vicia faba L. creole), was evaluated for the first time as a material capable of improving glucose homeostasis in diabetic conditions. Methods: The RS ingredient investigated (with a reduced digestibility of 50%) was tested in a Wistar rat model with induced diabetes, fed with a 15 or 30% replacement of RS ingredient in the diet. Diverse nutritional and biomarkers were analysed. Results: As a result of the reduced digestibility of the RS ingredient, diabetic animals fed with RS replacement (15% or 30%) showed attenuated postprandial hyperglycemia responses, reducing the hyperglycemic condition close to 29% compared to non-treated diabetic animals (24.56 ± 7.50 and 25.02 ± 3.54 vs. 34.65 ± 1.89 mmol/L, respectively). In addition, fasting serum glucose levels were significantly reduced (22%). Other biochemical parameters associated with glucose metabolism, such as glycosylated hemoglobin and AGEs levels, also improved. Furthermore, significant improvements in nutritional parameters (such as weight gain) and a lower insulin resistance index were determined. In contrast, no clear effects were observed in lipid metabolism and oxidative stress biomarkers in the treated group. Conclusions: The results of this research suggest that the retrograded starch from creole beans evaluated could be a potential functional food ingredient capable of enhancing glucose homeostasis in diabetic conditions. Full article