Search Results (628)

Polynucleotide (PN) and polydeoxyribonucleotide (PDRN) are DNA-derived biopolymers increasingly recognized for their potential in dermatology. Despite their structural similarities, PN and PDRN exhibit distinct functions due to differences in polymer length and molecular weight. PN, composed of longer DNA fragments, plays a key role in extracellular matrix remodeling. Conversely, PDRN, composed of relatively shorter oligonucleotide sequences than those of PN, enhances skin condition through adenosine receptor activations and supports nucleotide synthesis via both the salvage and de novo pathways. This review provides a critical comparison of the molecular characteristics and functions of PN and PDRN with particular emphasis on their dermatological applications. By delineating their respective roles in esthetic and regenerative medicine, we aim to highlight recent advances that may guide the development of optimized treatment strategies and foster evidence-based clinical practice. Full article

Recently, a number of natural biologically active substances have been proven to be attractive alternatives to conventional anticancer medicine or as adjuvants in contemporary combination therapies. Although lignin-based materials were previously accepted as waste materials with limited usefulness, recent studies increasingly report the possibility of their use for novel applications in various industrial branches, including biomedicine. In this regard, the safety, efficiency, advantages and limitations of lignin compounds for in vitro/in vivo applications remain poorly studied and described. This study was carried out to investigate the possibility of using newly synthesized, alkali lignin-based micro-/nano-biopolymer formulations (Lignin@Formulations/L@F) as carriers for substances with antioxidant and/or anticancer effectiveness. Moreover, we tried to assess the opportunity for using an electro-assisted approach for achieving improved intracellular internalization. An investigation was conducted on an in vitro panel of breast cell lines, namely two breast cancer lines with different metastatic potentials and one non-tumorigenic line as a control. The characterization of all tested formulations was performed via DLS (dynamic light scattering) analysis. We developed an improved separation procedure via size/charge unification for all types of Lignin@Formulations. Moreover, in vitro applications were investigated. The results demonstrate that compared to healthy breast cells, both tested cancer lines exhibited slight sensitivity after treatment with different formulations (empty or loaded with antioxidant substances). This effect was also enhanced after applying electric pulses. L@F loaded with Quercetin was also explored only on the highly metastatic cancer cell line as a model for the breast cancer type most aggressive and non-responsive to traditional treatments. All obtained data suggest that the tested formulations have potential as carriers for the electro-assisted delivery of natural antioxidants such as Quercetin. Full article

Kefiran, the extracellular polysaccharide produced from the Generally Recognized as Safe (GRAS) bacteria in kefir grains, with its well-documented functional and health-promoting properties, constitutes a promising biopolymer with a variety of possible uses. Its compatibility with other biopolymers, such as milk proteins, and its ability to form standalone cryogels allow it to be utilized for the fabrication of films with improved properties for applications in the food and biomedical–pharmaceutical industries. In the present work, the properties of kefiran films were investigated in the presence of milk proteins (sodium caseinate, native and heat-treated whey proteins, and their mixtures), alongside glycerol (as a plasticizer) and cryo-treatment of the film-forming solution prior to drying. A total of 24 kefiran films were fabricated and studied for their physical (thickness, moisture content, water solubility, color parameters and vapor adsorption), mechanical (tensile strength and elongation at break), and optical properties. Milk proteins increased film thickness, solubility and tensile strength and reduced water vapor adsorption. The hygroscopic effect of glycerol was mitigated in the presence of milk proteins and/or the application of cryo-treatment. Glycerol was the most effective at reducing the films’ opacity. Heat treatment of whey proteins proved to be the most effective in increasing film tensile strength, reducing, at the same time, the elongation at break, while sodium caseinates in combination with cryo-treatment resulted in films with high tensile strength and the highest elongation at break. Cryo-treatment, carried out in the present study through freezing followed by gradual thawing of the film-forming solution, proved to be the most effective factor in decreasing film roughness. Based on our results, proper selection of the film-forming solution composition and its treatment prior to drying can result in kefiran–glycerol films with favorable properties for particular applications. Full article

Background/Objectives: Dental practitioners widely use dental implants to treat traumatic cases. Titanium implants are currently the most popular choice among dental practitioners and surgeons. The discovery of newer polymeric materials is also influencing the interest of dental professionals in alternative options. A comparative study between existing titanium implants and newer polymeric materials can enhance professionals’ ability to select the most suitable implant for a patient’s treatment. This study aimed to investigate material property advantages of high-performance thermoplastic biopolymers such as PEEK and PEKK, as compared to the time-tested titanium implants, and to find the most suitable and economically fit implant material. Methods: Three distinct implant material properties were assigned—PEEK, PEKK, and commercially pure titanium (CP Ti-55)—to dental implants measuring 5.5 mm by 9 mm, along with two distinct titanium (TI6AL4V) abutments. Twelve three-dimensional (3D) models of bone blocks, representing the mandibular right molar area with Osseo-integrated implants were created. The implant, abutment, and screw were assumed to be linear; elastic, isotropic, and orthotropic properties were attributed to the cancellous and cortical bone. Twelve model sets underwent a three-dimensional finite element analysis to evaluate von Mises stress and total deformation under 250 N vertical and oblique (30 degree) loads on the top surface of each abutment. Results: The study revealed that the time-tested titanium implant outperforms PEEK and PEKK in terms of marginal bone preservation, while PEEK outperforms PEKK. Conclusions: This study will assist dental practitioners in selecting implants from a variety of available materials and will aid researchers in their future research. Full article

The demand for anion exchange membranes (AEMs) is growing due to their applications in water electrolysis, CO₂ reduction conversion and fuel cells, as well as water treatment, driven by the increasing energy demand and the need for a sustainable future. However, current AEMs still face challenges, such as insufficient permeability and stability in strongly acidic or alkaline media, which limit their durability and the sustainability of membrane fabrication. In this study, polyvinyl alcohol (PVA) and chitosan (CS) biopolymers are selected for membrane preparation. Zinc oxide (ZnO) and porous organic polymer (POP) nanoparticles are also introduced within the PVA-CS polymer blends to make mixed-matrix membranes (MMMs) with increased OH⁻ transport sites. The membranes are characterized based on typical properties for AEM applications, such as thickness, water uptake, KOH uptake, Cl⁻ and OH⁻ permeability and ion exchange capacity (IEC). The OH⁻ transport of the PVA-CS blend is increased by at least 94.2% compared with commercial membranes. The incorporation of non-porous ZnO and porous POP nanoparticles into the polymer blend does not compromise the OH⁻ transport properties. On the contrary, ZnO nanoparticles enhance the membrane’s water retention capacity, provide basic surface sites that facilitate hydroxide ion conduction and reinforce the mechanical and thermal stability. In parallel, POPs introduce a highly porous architecture that increases the internal surface area and promotes the formation of continuous hydrated pathways, essential to efficient OH⁻ mobility. Furthermore, the presence of POPs also contributes to reinforcing the mechanical integrity of the membrane. Thus, PVA-CS bio-based membranes are a promising alternative to conventional ion exchange membranes for various applications. Full article

Moringa oleifera (MO), a nutritionally and pharmacologically potent species, is emerging as a sustainable candidate for applications across bioenergy, agriculture, textiles, pharmaceuticals, and biomedicine. This review explores recent advances in MO-based biotechnologies, highlighting novel extraction methods, green nanotechnology, and clinical trial findings. Although MO’s resilience offers promise for climate-smart agriculture and public health, challenges remain in standardizing cultivation and verifying therapeutic claims. This work underscores MO’s translational potential and the need for integrative, interdisciplinary research. MO is used in advanced materials, like electrospun fibers and biopolymers, showing filtration, antibacterial, anti-inflammatory, and antioxidant properties—important for the biomedical industry and environmental remediation. In textiles, it serves as an eco-friendly alternative for wastewater treatment and yarn sizing. Biotechnological advancements, such as genome sequencing and in vitro culture, enhance traits and metabolite production. MO supports green biotechnology through sustainable agriculture, nanomaterials, and biocomposites. MO shows potential for disease management, immune support, metabolic health, and dental care, but requires further clinical trials for validation. Its resilience is suitable for land restoration and food security in arid areas. AI and deep learning enhance Moringa breeding, allowing for faster, cost-effective development of improved varieties. MO’s diverse applications establish it as a key element for sustainable development in arid regions. Full article

The food industry consumes large amounts of water across various processes, and generates wastewater characterized by parameters like biochemical oxygen demand, chemical oxygen demand, pH, suspended solids, and nutrients. To meet environmental standards and enable reuse or valorization, treatment methods such as physicochemical, biological, and membrane-based processes are applied. This review focuses on the valorization of food industry wastewater in the biotechnological production of high-value products, with an emphasis on starch-rich wastewater, wineries and confectionery industry wastewater, and with a focus on new technologies for reduces environmental burden but also supports circular economy principles. Starch-rich wastewaters, particularly those generated by the potato processing industry, offer considerable potential for biotechnological valorization due to their high content of soluble starch, proteins, organic acids, minerals, and lipids. These effluents can be efficiently converted by various fungi (e.g., Aspergillus, Trichoderma) and yeasts (e.g., Rhodotorula, Candida) into value-added products such as lipids for biodiesel, organic acids, microbial proteins, carotenoids, and biofungicides. Similarly, winery wastewaters, characterized by elevated concentrations of sugars and polyphenols, have been successfully utilized as medium for microbial cultivation and product synthesis. Microorganisms belonging to the genera Aspergillus, Trichoderma, Chlorella, Klebsiella, and Xanthomonas have demonstrated the ability to transform these effluents into biofuels, microbial biomass, biopolymers, and proteins, contributing to sustainable bioprocess development. Additionally, wastewater from the confectionery industry, rich in sugars, proteins, and lipids, serves as a favorable fermentation medium for the production of xanthan gum, bioethanol, biopesticides, and bioplastics (e.g., PHA and PHB). Microorganisms of the genera Xanthomonas, Bacillus, Zymomonas, and Cupriavidus are commonly employed in these processes. Although there are still certain regulatory issues, research gaps, and the need for more detailed economic analysis and kinetics of such production, we can conclude that this type of biotechnological production on waste streams has great potential, contributing to environmental sustainability and advancing the principles of the circular economy. Full article

In this work, the electrospinning technique was used to prepare novel polymeric composite fibers containing Te-doped bioactive glass powders. Bioactive glass powders containing tellurium (STe5 glass) were chosen as fillers for the composites, owing to their bioactive, antibacterial, and antioxidant properties. The biopolymer poly (ϵ-caprolactone) (PCL) and acetic acid (AA) were used as raw materials for the preparation of the polymeric matrix. FESEM analysis confirmed a good incorporation of the glass powders in the polymeric fibers, of up to 20% by weight. Wettability, mechanical, in vitro stability and preliminary antibacterial tests were also performed. The results showed that the treatment in AA did not affect the bioactivity of the glass powders, the presence of STe5 powders in PCL enhanced the wettability of the fibers, and mechanical properties improved by increasing the amount of STe5 powders, as well as the antibacterial effect. Therefore, the obtained materials appear promising for developing multifunctional composite materials for applications in tissue engineering. Full article

Nanotechnology, as a burgeoning interdisciplinary field, has a significant application potential in food nutrition and human health due to its distinctive structural characteristics and surface effects. This paper methodically examines the recent advancements in nanotechnology pertaining to food production, functional nutrition delivery, and health intervention. In food manufacturing, nanoparticles have markedly enhanced food safety and quality stability via technologies such as antimicrobial packaging, intelligent sensing, and processing optimization. Nutritional science has used nanocarrier-based delivery systems, like liposomes, nanoemulsions, and biopolymer particles, to make active substances easier for the body to access and target. Nanotechnology offers innovative approaches for chronic illness prevention and individualized treatment in health interventions by enabling accurate nutritional delivery and functional regulation. Nonetheless, the use of nanotechnology encounters hurdles, including safety evaluations and regulatory concerns that require additional investigation. Future research should concentrate on refining the preparation process of nanomaterials, conducting comprehensive examinations of their metabolic mechanisms within the human body, and enhancing pertinent safety standards to facilitate the sustainable advancement of nanotechnology in food production, nutrition, and health. Full article

The olive oil sector constitutes a fundamental pillar in the Mediterranean region from socio-economic and cultural perspectives. Nonetheless, it produces significant amounts of waste, leading to numerous environmental issues. These waste streams contain valuable compounds that can be recovered and utilized as inputs for various applications. This study introduces a novel value chain for olive wastes, focused on extracting lignin from olive pomace by ionic liquids and polyphenols from olive mill wastewater, which are then incorporated as hybrid nanoparticles in the formulation of an innovative starch-based biofertilizer. This biofertilizer, obtained by using residual wastewater as a source of soluble nitrogen, acting at the same time as a plasticizer for the biopolymer, was demonstrated to surpass traditional NPK biofertilizers’ efficiency, allowing for root growth and foliage in drought conditions. In order to recognize the environmental impact due to its production and align it with the technical output, the circularity and environmental performance of the proposed system were innovatively evaluated through a combination of Life Cycle Assessment (LCA) and the Material Circularity Indicator (MCI). LCA results indicated that the initial upcycling process was potentially characterized by significant hot spots, primarily related to energy consumption (>0.70 kWh/kg of water) during the early processing stages. As a result, the LCA score of this preliminary version of the biofertilizer may be higher than that of conventional commercial products, due to reliance on thermal processes for water removal and the substantial contribution (56%) of lignin/polyphenol precursors to the total LCA score. Replacing energy-intensive thermal treatments with more efficient alternatives represents a critical area for improvement. The MCI value of 0.84 indicates limited potential for further enhancement. Full article

Starch extracted from malanga (Colocasia esculenta) is a biopolymer with considerable industrial potential thanks to its high starch content (70–80% on a dry basis) and small granule size, which give it distinctive functional properties. To expand its applications in advanced processes such as encapsulation, it is necessary to modify its structural and physicochemical characteristics. This study evaluated the effects of ultrasound (US) and chemical cross-linking (CL) on the properties of this starch. US was applied at various times and amplitudes, while CL was performed using sodium trimetaphosphate and sodium tripolyphosphate, with sodium sulfate as a catalyst. US treatment reduced particle size and increased amylose content, resulting in lower viscosity and gelatinization temperature, without affecting granule morphology. Meanwhile, CL induced phosphate linkages between starch chains, promoting aggregation and reducing amylose content and enthalpy, but increasing the gelatinization temperature. The modified starches exhibited low syneresis, making them potentially suitable for products such as pastas, baby foods, and jams. Additionally, ultrasound modification enabled the production of fine starch microparticles, which could be applied in the microencapsulation of bioactive compounds in the food and pharmaceutical industries. These findings suggest that modified malanga starch can serve as a functional and sustainable alternative in industrial applications. Full article

The textile industry is progressively shifting towards more sustainable solutions, particularly in the field of printing technologies. This study reports the development and evaluation of water-based pigment inks formulated with bio-based pigments derived from intermediates produced via bacterial fermentation. Two pigments—indigo (blue) and quinacridone (red)—were incorporated into ink formulations and applied on cotton and polyester fabrics through valve-jet inkjet printing (ChromoJet). The physical properties of the inks were analyzed to ensure compatibility with the equipment, and printed fabrics were assessed as to their color fastness to washing, rubbing, artificial weathering, and artificial light. The results highlight the good performance of the bio-based inks, with excellent light and weathering fastness and satisfactory wash and rub resistance. The effect of different pre-treatments, including a biopolymer and a synthetic binder, was also investigated. Notably, the biopolymer pre-treatment enhanced pigment fixation on cotton, while the synthetic binder improved wash fastness on polyester. These findings support the integration of biotechnologically sourced pigments into eco-friendly textile digital printing workflows. Full article

Soil improvement is one of the fundamental practices in civil engineering, with a long-standing history. In today’s context, the rapidly increasing demand for construction driven by urbanization has further emphasized the necessity and significance of soil stabilization techniques. This study aims to determine the optimum parameters for improving sandy soils by incorporating sodium alginate (SA) as a biopolymer additive into the microbial calcium carbonate precipitation (MICP) process. Sand types S1, S2, and S3, each with distinct particle size distributions, were selected, and the specimens were prepared at medium relative density. Three distinct approaches, MICP, SA, and MICP + SA, were tested for comparison. Additionally, two different improvement methods, injection and mixing, were applied to investigate their effects on the geotechnical properties of the soils. In this context, hydraulic conductivity, unconfined compressive strength (UCS), and calcite content tests, as well as scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) analyses, were performed to assess the changes in soil behavior. SA contributed positively to the overall efficiency of the MICP process. The study highlights SA-assisted MICP as an alternative that enhances the microstructural integrity of treated soils and responds to the environmental limitations of conventional methods through sustainable innovation. Full article

This research work focused on the development of an adsorbent biocomposite material based on polyhydroxybutyrate (PHB) and cellulose acetate derived from sugarcane (Saccharum officinarum) fibre, through cellulose acetylation. The resulting material represents both an accessible and effective alternative for the treatment and remediation of water contaminated with heavy metals, such as Ni (II). The biocomposite was prepared by blending cellulose acetate (CA) with the biopolymer PHB using the solvent-casting method. The resulting biocomposite exhibited a point of zero charge (pHpzc) of 5.6. The material was characterised by FTIR, TGA-DSC, and SEM analyses. The results revealed that the interaction between Ni (II) ions and the biocomposite is favoured by the presence of functional groups, such as –OH, C=O, and N–H, which act as active adsorption sites on the material’s surface, enabling efficient interaction with the metal ions. Adsorption kinetics studies revealed that the biocomposite achieved an optimal adsorption capacity of 5.042 mg/g at pH 6 and an initial Ni (II) concentration of 35 mg/L, corresponding to a removal efficiency of 86.44%. Finally, an analysis of the kinetic and isotherm models indicated that the experimental data best fit the pseudo-second-order kinetic model and the Freundlich isotherm. Full article

In recent years, membranes have found extensive applications, primarily in wastewater purification and food packaging. However, petroleum-based membranes can be detrimental to the environment. For this reason, extensive studies are being conducted to identify environmentally friendly substitutes for the materials used in membrane composition. Among these materials, polylactic acid (PLA) and poly(3-hydroxybutyrate) (PHB) are two bio-sourced and biodegradable polymers that can be derived from lignocellulosic waste. These polymers also possess suitable characteristics, such as thermal resistance and mechanical strength, which make them potential candidates for replacing conventional plastics. This study provides an overview of recent advances in the production of PLA and PHB, with a focus on their extraction from lignocellulosic biomass, as well as the recent applications of these two biodegradable polymers as sustainable materials in membrane manufacturing. The advantages and limitations of membranes produced from these materials are also summarized. Lastly, an analysis of future trends is provided concerning new sources, production possibilities, and potential applications in water treatment (mainly for metal ions separation), gas separation, oil–water separation, medical applications, drug release control, and food packaging. Full article