Search Results (292)

Background: There are only a few available options for early rehabilitation of severely impaired individuals who must remain bedbound, as most exercise paradigms focus on out-of-bed exercises. To enable these individuals to exercise, we developed a novel hybrid rehabilitation system combining a brain–computer interface (BCI), functional electrical stimulation (FES), and a robotic device. Methods: The BCI assessed the presence of a movement-related cortical potential (MRCP) and triggered the administration of FES to produce movement of the lower limb. The exercise trajectory was supported by the robotic device. To demonstrate the system, an experiment was conducted in an out-of-lab setting by ten able-bodied participants. During exercise, the performance of the BCI was assessed, and the participants evaluated the system using the NASA Task Load Index, Intrinsic Motivation Inventory, and by answering a few subjective questions. Results: The BCI reached a true positive rate of 62.6 ± 9.2% and, on average, predicted the movement initiation 595 ± 129 ms prior to the MRCP peak negativity. All questionnaires showed favorable outcomes for the use of the system. Conclusions: The developed system was usable by all participants, but its clinical feasibility is uncertain due to the total time required for setting up the system. Full article

Nowadays, society is becoming increasingly committed to traveling by plane for work, tourism, and leisure in general. However, either due to internal, specific factors or to external determinants, like terrorism and climate changes, a growing number of travelers have experienced the so-called fear of flying, a persistent, irrational fear of flight-related situations for which a clear, efficacious therapy does not yet exist. Based on the usual interaction with the surrounding environment, conducted by means of the five human senses, and particularly on the neurophysiological pathway followed by the chemical senses, in this study, we revise the findings in the related literature on the topic, proposing an alternative way to alleviate the anxiety related to the fear of flight. This is based on chemosensory stimulation being applied directly during a flight and is possibly concerned with the consumption of meals, an usual activity performed onboard. After an introductory section aimed at understanding the problem, we present some studies related to chemosensory perception during the flight, highlighting the specificities of the scenarios, followed by a description of findings related to the meals proposed by flight companies in this context, and finally wrapping up the possible alternative approaches that could be conducted by such providers to alleviate the fear of flying condition through chemosensory stimulation vehiculated by meals, and enhance the quality of flight experience related to food consumption onboard. Full article

Bioelectronics for wearable and implantable biomedical devices has attracted significant attention due to its potential for continuous health monitoring, early disease diagnosis, and real-time therapeutic interventions. Among the various materials explored for bioelectronic applications, hydrogels derived from natural biopolymers have emerged as highly promising candidates, owing to their inherent biocompatibility, mechanical compliance akin to biological tissues, and tunable structural properties. This review provides a comprehensive overview of recent advancements in the design and application of protein-based hydrogels, including gelatin, collagen, silk fibroin, and gluten, as well as carbohydrate-based hydrogels such as chitosan, cellulose, alginate, and starch. Particular emphasis is placed on elucidating their intrinsic material characteristics, modification strategies to improve electrical and mechanical performance, and their applicability for bioelectronic interfaces. The review further explores their diverse applications in physiological and biochemical signal sensing, bioelectric signal recording, and electrical stimulation. Finally, current challenges and future perspectives are discussed to guide the ongoing innovation of hydrogel-based systems for next-generation bioelectronic technologies. Full article

The increasing prevalence of antibiotic-resistant bacteria has stimulated the search for alternative antimicrobial agents with greater efficacy, low toxicity, and minimal resistance potential. Natural products, such as honey, propolis, and royal jelly, have shown promise due to their biological properties. The integration of natural products like honey and propolis in biomaterials represents a synergistic approach to combat the growing threat of resistant bacterial infections while improving wound care and soft tissue engineering applications. In the present work, we obtained sodium alginate films based on honey, propolis, royal jelly, and their mixture coated with chitosan for soft tissue regeneration. SEM showed that adding bee products altered surface morphology, affecting roughness, porosity, and microstructure. Spectral analysis confirmed specific chemical bonds, while thermal studies indicated a good stability up to 115 °C. The antimicrobial activity was evaluated against Gram-positive (Enterococcus faecalis, Staphylococcus aureus), Gram-negative (Escherichia coli, Pseudomonas aeruginosa) and yeast strains (Candida albicans), with growth inhibition zone diameters up to 12 mm. In vitro cytotoxicity studies, made on human gingival fibroblasts, suggested good biocompatibility. Antimicrobial assays showed that films containing propolis tincture, alone or as a mixture, were most effective against pathogens. Future research will focus on formulation optimization for biomedical use. Full article

Photoacoustic imaging is an advanced biomedical imaging technique that has been widely developed and applied in diverse biomedical studies. By generating optical-absorption-based signals with ultrasound resolution, it enables in vivo visualization of molecular functional information in biological tissues. Extensive research has been conducted to develop the multispectral light sources required for functional photoacoustic imaging. Among the various approaches, multispectral light sources generated using stimulated Raman scattering have shown considerable promise, particularly in photoacoustic microscopy, where achieving multispectral illumination remains challenging. This review summarizes photoacoustic imaging systems that employ stimulated Raman scattering for multispectral light sources and delves into their configurations and applications in the functional analyses of biological tissues. In addition, the review discusses the future directions of multispectral light sources by comparing different technologies based on key factors such as wavelength tunability, repetition rate, and power, which critically affect the accuracy and quality of multispectral photoacoustic imaging. Full article

Background/Objectives: Chlamydia trachomatis (Ct) is the leading bacterial cause of sexually transmitted infection globally. If undiagnosed or left untreated, these infections can lead to serious complications such as infertility, ectopic pregnancies, and chronic pelvic pain. Despite the high prevalence and potential for serious health complications, no vaccine has been licensed. Pigs offer a valuable biomedical model for chlamydia research: they have an overall high degree of similarity to humans and serve as natural hosts for Chlamydia suis (Cs), a close relative of Ct. Thus, in this study, the pig model was used to evaluate a vaccine candidate against Ct. Methods: The vaccine candidate consists of chlamydial-protease-like activity factor (CPAF) protein adjuvanted with STING (Stimulator of Interferon Genes) pathway agonist cyclic-di-AMP (c-di-AMP). Pigs received two doses intramuscularly followed by two intranasal doses. Each week, the systemic T cell response was assessed via IFN-γ and IL-17 ELISpots, as well as multi-parameter flow cytometry on 0, 14, and 28 days post vaccination (dpv). The humoral immune response was analyzed by measuring CPAF-specific antibody levels and avidity via ELISAs. Results: Vaccination with c-di-AMP adjuvanted CPAF triggered low-level systemic IFN-γ and multifunctional IFN-γ⁺TNF-α⁺ CD4 T cell responses. Despite the rather low systemic effector cytokine production, robust anti-CPAF IgG responses were detected in serum, vaginal swab eluates, and oviduct flushes. Genital Ct challenge 42 dpv resulted in only transient infection, precluding a confident assessment of vaccine efficacy of the tested CPAF/c-di-AMP vaccine candidate. However, after challenge, vaccinated pigs exhibited boosted systemic anti-CPAF IFN-γ and mucosal IgG responses compared to unvaccinated pigs. Conclusions: Thus, while vaccine efficacy remains elusive, the CPAF/c-di-AMP vaccine candidate was immunogenic: it elicited a low-level systemic cell-mediated response and robust humoral immune responses. Future studies will incorporate a STING agonist directly conjugated to CPAF as well as addition of other Th1-inducing adjuvants to enhance cellular immunity. Full article

The rising demand for alternative solutions to diabetes mellitus has prompted significant interest in the exploration of plant-derived anti-diabetic compounds, especially within a circular economy framework that seeks sustainable and profitable reuse options. In this context, red (RSGO) and white (WGSO) grape seed oils, by-products of Sicilian vineyards, were prepared, analyzed for their fatty acid, polyphenol, carotenoid, and chlorophyll content, and evaluated for their glucose-lowering ability on HepG2 cells. Utilizing cytochemical techniques, flow cytometry, and protein blotting, we explored the effects of non-toxic oil dilutions on (i) glycogen storage, (ii) glucose consumption/uptake, (iii) GLUT-2, GLUT-4, and hepatocyte nuclear factor-1α (HNF1α) expression levels, and (iv) AMP-activated protein kinase (AMPK), insulin receptor substrate-1 (IRS-1), AKT, and PKCζ phosphorylation states, which are involved in insulin-mediated and -independent regulation of GLUT-4 membrane exposure. RGSO and WGSO, despite adopting slightly varying molecular strategies, were both proven to be effective stimulators of glucose absorption and glycogenesis. Specifically, RSGO promoted GLUT-2 and GLUT-4 up-regulation, whereas the WGSO-induced effect was associated with an increase in GLUT-4 levels alone. Moreover, the oils activated both pathways responsible for GLUT-4 translocation. Therefore, these wine-making residues have substantial potential as anti-diabetic solutions, holding promise for integration into the biomedical and food sectors. Full article

Antimicrobial resistance (AMR) is an urgent global health threat with many anthropogenic drivers outside of healthcare. The impacts of modern agriculture on human health are manifold, from the food systems and dietary patterns they support to the less apparent effects of environmental stresses and biodiversity loss in ecosystems. Intensive practices, such as chemical fertilizers, pesticides, and herbicides, induce abiotic stresses that deplete biodiversity and drive AMR in soil and aquatic microbiomes. The overuse of antibiotics in livestock production is another major driver of AMR. Changes in weather patterns due to climate change have the potential to exacerbate these issues as warmer and wetter weather increases the potential for bacterial infection. While practices exist to address healthcare-associated drivers, the impact of agriculture and environmental destruction are not widely appreciated in healthcare and biomedical sciences. It is imperative that healthcare professionals and public health experts understand these connections to properly address the emergent issue of AMR. This review aims to summarize the current data on important agricultural and environmental drivers of AMR for educational purposes, to fill gaps in knowledge, and to improve current practices and stimulate further research. Full article

Low temperature plasmas (LTPs) generated at atmospheric pressure and room temperature have gained increasing attention in biomedical research due to their ability to control cellular behavior through the production of reactive oxygen and nitrogen species (RONS), electric fields, and UV radiation. Among several LTP configurations, dielectric barrier discharge (DBD) plasma has been extensively studied for its ability to stimulate controlled biological effects while maintaining low gas temperature, making it suitable for cell-based applications. This study designed a novel spiral-wound DBD plasma device to investigate the voltage-dependent effects of plasma discharge on DBC1.2 epithelial cells. Plasma was applied at 2 kV_p-p, 3 kV_p-p, and 4 kV_p-p to evaluate its effect on cellular permeability, mitochondrial activity, viability, and apoptosis. FITC-dextran-70 (FD-70, MW: 70 kDa) was used as a model permeation marker to assess cellular uptake. The results showed a voltage-dependent increase in FD-70 uptake, suggesting improved plasma-assisted drug delivery. The cell mitochondrial activity, evaluated with a MT-1 MitoMP detection kit, revealed that plasma exposure at 2 kV_p-p and 3 kV_p-p slightly enhanced mitochondrial membrane potential (MMP), signifying increased metabolic and mitochondrial activity, whereas exposure at 4 kV_p-p led to a reduction in MMP, suggesting oxidative stress and early apoptosis. Early and late apoptosis was further assessed using FITC Annexin-V and propidium iodide (PI). The results showed enhanced cell viability and a reduced apoptotic cell at 2 kV_p-p and 3 kV_p-p plasma exposure when compared to the control. However, at 4 kV, there was a decline in cell viability and an increase in apoptosis, suggesting a shift towards plasma-induced cytotoxicity. This study established a safe plasma exposure threshold for DBC1.2 cells and explored the potential use of a spiral-wound DBD plasma device for biomedical applications, particularly in drug delivery and cell modulation. Full article

The application background of mussel-inspired materials is based on the unique underwater adhesive ability of marine mussels, which has inspired researchers to develop bionic materials with strong adhesion, self-healing ability, biocompatibility, and environmental friendliness. Specifically, 3, 4-dihydroxyphenylalanine (DOPA) in mussel byssus is able to form non-covalent forces on a variety of surfaces, which are critical for the mussel’s underwater adhesion and enable the mussel-inspired material to dissipate energy and repair itself under external forces. Mussel-inspired hydrogels are ideal medical adhesive materials due to their unique physical and chemical properties, such as excellent tissue adhesion, hemostasis and bacteriostasis, biosafety, and plasticity. This paper reviewed chitosan, cellulose, hyaluronic acid, gelatin, alginate, and other biomedical materials and discussed the advanced functions of mussel-inspired hydrogels as wound dressings, including antibacterial, anti-inflammatory, and antioxidant properties, adhesion and hemostasis, material transport, self-healing, stimulating response, and so on. At the same time, the technical challenges and limitations of the biomimetic mussel hydrogel in biomedical applications were further discussed, and its potential solutions and future research developments in the field of biomedicine were highlighted. Full article

Drug delivery systems enhance drug efficacy while minimizing side effects. Liposomes, as well-studied and clinically approved carriers, offer biodegradability, biocompatibility, and low toxicity, making them suitable for delivering various pharmacological agents. Granulocyte colony-stimulating factor (G-CSF), a key growth factor, has shown therapeutic potential, particularly in infertility treatment. It effectively manages chronic and refractory endometritis by improving endometrial receptivity and increasing embryo implantation success. Studies indicate that G-CSF promotes endometrial growth and enhances the uterine microenvironment, benefiting patients with recurrent implantation failures and chronic endometritis. Encapsulation of G-CSF in liposomes enhances its stability, bioavailability, and controlled release. G-CSF-loaded liposomes were prepared using passive loading via the thin-film hydration method. The size of the liposomes, polydispersity index (PDI), and zeta potential were determined using dynamic and electrophoretic light scattering methods, and the encapsulation efficiency was measured using high-performance liquid chromatography. The morphology of the liposomes was established and confirmed using cryogenic transmission electron microscopy. The cytocompatibility of the G-CSF-loaded liposomes was evaluated on human dermal fibroblasts using an MTT assay. The G-CSF-loaded liposomes had an average particle size of 161.9 ± 9.9 nm, a PDI of 0.261 ± 0.03, and a zeta potential of +2.09 ± 0.10 mV, exhibiting high physical stability during long-term storage at +4 °C and 60% humidity. The passive loading method resulted in a 52.37 ± 3.64% encapsulation efficiency of the active substance. The analysis of cell viability revealed no cytotoxicity toward liposomes loaded with G-CSF and demonstrated a dose-dependent effect on the viability of human dermal fibroblasts. Thus, the obtained data confirm the successful preparation of G-CSF-loaded liposomes. However, to fully understand their effectiveness in biomedical applications, further research is needed, including an evaluation of their effectiveness in vivo. Such studies will help in determining the potential of these formulations for specific biomedical purposes and evaluating their safety and efficacy in living systems. Full article

Despite the substantial advances in cancer therapies, developing safe and effective treatment methodologies is critical. Natural (plant-derived compounds), such as flavonoids, might be crucial in developing a safe treatment methodology without toxicity toward healthy tissues. Prunin is a flavonoid with the potential to be used in biomedical applications. Prunin has yet to undergo thorough scientific research, and its precise molecular mechanisms of action remain largely unexplored. This review summarizes the therapeutic potential of prunin for the first time, focusing on its underlying mechanisms as an anticancer compound. Prunin has gained significant attention due to its antioxidant, anti-inflammatory, and anticancer effects. This review aims to unlock how prunin functions at the molecular level to exert its anticancer effects, primarily modulating key cellular pathways. Furthermore, we have discussed the prunin’s potential as an adjunctive therapy with conventional treatments, highlighting its ability to strengthen treatment responses while decreasing drug resistance. Moreover, the discussion probes into innovative delivery methods, particularly nanoformulations, that might address prunin’s bioavailability, solubility, and stability limitations and optimize its therapeutic application. By providing a comprehensive analysis of prunin’s properties, this review aims to stimulate further exploration of using prunin as an anticancer agent, thereby progressing the development of targeted, selective, safe, and effective therapeutic methods. Full article

Magnesium and its rare-earth alloys are extensively studied for their lightweight properties and high specific strength, making them attractive for aerospace, automotive, and biomedical applications. However, their hexagonal close-packed structure leads to a strong basal texture, limiting plasticity and formability at room temperature. Considerable research has been devoted to texture control strategies, including alloying, thermomechanical processing, and recrystallization mechanisms, yet a comprehensive understanding of their effects remains an ongoing research focus. This review summarizes recent advances in texture regulation of rare-earth magnesium alloys, focusing on the role of RE elements (Gd, Y, Nd, Ce) and non-RE elements (Zn, Ca) in modifying basal texture and enhancing mechanical properties. The influence of key processing techniques, such as extrusion, rolling, equal channel angular pressing, and rotary shear extrusion, is discussed in relation to their effects on recrystallization behavior. Additionally, the mechanisms governing texture evolution, including continuous dynamic recrystallization, discontinuous dynamic recrystallization (DDRX), and particle-stimulated nucleation, are critically examined. By integrating recent findings, this review provides a systematic perspective on alloying strategies, processing conditions, and recrystallization pathways, offering valuable insights for the development of high-performance magnesium alloys with improved formability and mechanical properties. Full article

Tungsten disulfide (WS₂) nanoparticles have emerged in the biomedical field as potential theranostic agents due to their unique properties, including biocompatibility. However, their impact on the immune response remains unexplored. This study aimed to evaluate the effects of inorganic fullerene-like WS₂ (IF-WS₂) nanostructures on human peripheral blood mononuclear cells (PBMCs) in vitro. The study investigated several parameters to evaluate the effects of IF-WS₂ nanoparticles. Cytotoxicity was assessed by measuring cell viability, apoptosis, and necrosis. Internalization of IF-WS₂ by PBMCs was analyzed using morphological and flow cytometric techniques. Proliferation was studied in CellTrace Far Red-prestained total PBMCs stimulated with phytohemagglutinin (PHA) and in isolated T cell cultures stimulated with CD3/CD28-coated beads. Additionally, the production of cytokines and chemokines was measured in culture supernatants of total PBMCs and T cells. IF-WS₂ nanoparticles were non-cytotoxic up to a concentration of 200 µg/mL. Concentrations ≥25 µg/mL inhibited PHA-stimulated PBMC proliferation but did not affect T cell proliferation. Morphological and flow cytometric analysis demonstrated dose- and time-dependent internalization of IF-WS₂ by macrophages. Additionally, IF-WS₂ significantly reduced the production of pro-inflammatory cytokines (IL-1β, TNF-α, IL-8, MCP-1, and GRO-α) in PHA-stimulated PBMCs. Th1, Th17, and Th21 cytokines were downregulated, while Th2, Th9, and T regulatory cytokines were upregulated. In conclusion, this study demonstrated for the first time that pristine IF-WS₂ nanoparticles, at non-cytotoxic concentrations, exhibit notable anti-inflammatory and immunomodulatory properties on activated PBMCs in vitro. Full article

Drug delivery systems have revolutionized traditional drug administration methods by addressing various challenges, such as enhancing drug solubility, prolonging effectiveness, minimizing adverse effects, and preserving potency. Nanotechnology-based drug delivery systems, particularly nanoparticles (NPs) and nanofibers (NFs), have emerged as promising solutions for biomedicine delivery. NFs, with their ability to mimic the porous and fibrous structures of biological tissues, have garnered significant interest in drug-delivering applications. Biopolymers such as gelatin (Ge) and chitosan (CH) have gained much more attention due to their biocompatibility, biodegradability, and versatility in biomedical applications. CH exhibits exceptional biocompatibility, anti-bacterial activity, and wound healing capabilities, whereas Ge provides good biocompatibility and cell adhesion properties. Ge/CH-based NFs stimulate cellular connections and facilitate tissue regeneration owing to their structural resemblance to the extracellular matrix. This review explores the additive methods of preparation, including electrospinning, force pinning, and template synthesis, focusing on electrospinning and the factors influencing the fiber structure. The properties of Ge and CH, their role in drug release, formulation strategies, and characterization techniques for electrospun fibers are discussed. Furthermore, this review addresses applications in delivering active moieties in the management of orthopedics and wound healing with regulatory considerations, along with challenges related to them. Thus, the review aims to provide a comprehensive overview of the potential of Ge/CH-based NFs for drug delivery and biomedical applications. Full article