Search Results (258,578)

Cell-free biosensors harness the selectivity of cellular machinery without living cells’ constraints, offering advantages in environmental monitoring, medical diagnostics, and biotechnological applications. This review examines recent advances in cell-free biosensor development, highlighting their ability to detect diverse analytes including heavy metals, organic pollutants, pathogens, and clinical biomarkers with high sensitivity and specificity. We analyze technological innovations in cell-free protein synthesis optimization, preservation strategies, and field deployment methods that have enhanced sensitivity, and practical applicability. The integration of synthetic biology approaches has enabled complex signal processing, multiplexed detection, and novel sensor designs including riboswitches, split reporter systems, and metabolic sensing modules. Emerging materials such as supported lipid bilayers, hydrogels, and artificial cells are expanding biosensor capabilities through microcompartmentalization and electronic integration. Despite significant progress, challenges remain in standardization, sample interference mitigation, and cost reduction. Future opportunities include smartphone integration, enhanced preservation methods, and hybrid sensing platforms. Cell-free biosensors hold particular promise for point-of-care diagnostics in resource-limited settings, environmental monitoring applications, and food safety testing, representing essential tools for addressing global challenges in healthcare, environmental protection, and biosecurity. Full article

Art therapy serves as a crucial intervention modality for children with autism spectrum disorder (ASD), demonstrating unique value in emotional expression, sensory integration, and social communication. However, current practice presents critical challenges, including the disconnect between design expertise and clinical needs, unclear mechanisms of environmental factors’ impact on therapeutic outcomes, and insufficient evidence-based support for technology integration. Purpose: This study aimed to construct an evidence-based theoretical framework for art therapy environment design for children with autism, clarifying the relationship between environmental design elements and therapeutic effectiveness. Methodology: Based on the Web of Science database, this study employed a dual-track approach comprising bibliometric analysis and micro-qualitative content analysis to systematically examine the knowledge structure and developmental trends. Research hotspots were identified through keyword co-occurrence network analysis using CiteSpace, while 24 representative design cases were analyzed to gain insights into design concepts, emerging technologies, and implementation principles. Key Findings: Through keyword network visualization analysis, this study identified ten primary research clusters that were systematically categorized into four core design elements: sensory feedback design, behavioral guidance design, emotional resonance design, and therapeutic support design. A responsive therapeutic environment conceptual framework was proposed, encompassing four interconnected components based on the ABC model from positive psychology: emotional, sensory, environmental, and behavioral dimensions. Evidence-based design principles were established emphasizing child-centeredness, the promotion of multisensory expression, the achievement of dynamic feedback, and appropriate technology integration. Research Contribution: This research establishes theoretical connections between environmental design elements and art therapy effectiveness, providing a systematic design guidance framework for interdisciplinary teams, including environmental designers, clinical practitioners, technology developers, and healthcare administrators. The framework positions technology as a therapeutic mediator rather than a driver, ensuring technological integration supports rather than interferes with children’s natural creative impulses. This contributes to creating more effective environmental spaces for art therapy activities for children with autism while aligning with SDG3 goals for promoting mental health and reducing inequalities in therapeutic access. Full article

Background/Objectives: This study evaluated the real-world effectiveness of prophylactic Human Papillomavirus (HPV) vaccination in Korean women aged over 26 years, focusing on its impact on persistent HPV infection and disease progression. Methods: This multicenter prospective study analyzed data from the Korea HPV Cohort (2010–2021). After applying exclusion criteria, the final analytical cohort included 1,231 women aged ≥ 27 years with cytologic findings of atypical squamous cells of undetermined significance/low-grade squamous intraepithelial lesions and HPV infection. Propensity score matching was used to compare vaccinated (n = 340) and unvaccinated (n = 891) participants. After matching, 273 vaccinated and 273 unvaccinated individuals were included in the final analysis. The primary outcomes were persistent HPV infection and progression to biopsy-confirmed cervical intraepithelial neoplasia grade 2 or worse (CIN2+). Logistic and Cox regression models were employed, with additional age-stratified analyses. Results: Among women aged 27–39 years, vaccination was significantly associated with a 54% reduction in the odds of persistent HPV infection (odds ratio = 0.46; 95% CI: 0.22–0.96; p = 0.040). In the full cohort, vaccinated participants had a 62% lower risk of progression to CIN2+ compared with unvaccinated participants (hazard ratio = 0.38; 95% CI: 0.18–0.81; p = 0.011). Body mass index had a notable impact on HPV persistence in HPV 16/18 genotype groups. Conclusions: HPV vaccination effectively reduced persistent infection and progression to CIN2+ in Korean women, particularly those vaccinated before age 40. These findings support the age-extended HPV vaccination policies in South Korea. Full article

Landslide susceptibility modelling represents an efficient approach to enhance disaster management and mitigation strategies. The focus of this paper lies in the development of a landslide susceptibility evaluation in northwestern Tunisia using the Analytical Hierarchy Process (AHP) and Artificial Neural Network (ANN) approaches. The used database covers 286 landslides, including ten landslide factor maps: rainfall, slope, aspect, topographic roughness index, lithology, land use and land cover, distance from streams, drainage density, lineament density, and distance from roads. The AHP and ANN approaches were applied to classify the factors by analyzing the correlation relationship between landslide distribution and the significance of associated factors. The Landslide Susceptibility Index result reveals five susceptible zones organized from very low to very high risk, where the zones with the highest risks are associated with the combination of extreme amounts of rainfall and steep slope. The performance of the models was confirmed utilizing the area under the Relative Operating Characteristic (ROC) curves. The computed ROC curve (AUC) values (0.720 for ANN and 0.651 for AHP) convey the advantage of the ANN method compared to the AHP method. The overlay of the landslide inventory data locations of historical landslides and susceptibility maps shows the concordance of the results, which is in favor of the established model reliability. Full article

Discovering, analyzing, and finding a key to understanding the physiological and biochemical responses that Vitis vinifera L. undertakes against drought stress is of fundamental importance for this profitable crop. Today’s considerable climatic fluctuations force researchers and farmers to focus on this issue with solutions inclined to respect the ecosystem. In this academic work, we focused on describing the drought stress consequences on several parameters of secondary metabolites on Vitis vinifera leaves (quercetins, kaempferol, resveratrol, proline, and xanthophylls) and on some ecophysiological characteristics (e.g., water potential, stomatal conductance, and leaf temperature) to compare the answers that diverse agronomic management techniques (i.e., irrigation with and without zeolite, pure zeolite and no application) could instaurate in the metabolic pathway of this important crop with the aim to find convincing and thought-provoking responses to use this captivating and versatile mineral, the zeolite known as the “magic rock”. Stressed grapevines reached 56.80 mmol/m²s gs at veraison and a more negative stem Ψ (+10.63%) compared to plants with zeolite. Resveratrol, in the hottest season, fluctuated from 0.18–0.19 mg/g in zeolite treatments to 0.37 mg/g in stressed vines. Quercetins were inclined to accumulate in response to drought stress too. In fact, we recorded a peak of quercetin (3-O-glucoside + 3-O-glucuronide) of 11.20 mg/g at veraison in stressed plants. It is interesting to note how the pool of metabolites was often unchanged for plants treated with zeolite and for plants treated with water only, thus elevating this mineral to a “stress reliever”. Full article

Amaranthus is appointed as a common weed associated with crops. The research was designed to survey the Amaranth existence pattern throughout the Fayoum Depression, Egypt, accompanied with a community vegetation analysis. The study was extended to collect and analyze associated soil samples. The obtained results figured out the prevalence of dicot families, herb growth forms, therophyte followed by phanerophyte life forms, the Pantropical monoregional chorotype, and the Mediterranean and Sudano-Zambezian followed by the Irano-Turanian pluri-regional chorotype. Multilevel pattern analysis stated that Gossypium barbadense, Corchorus olitorius, Sorghum bicolor, Sesamum indicum, and Zea mays are indicator species most related to Amaranth occurrence and prediction. NMDS analysis denoting that the Ibshaway, Youssef Al Seddik, Itsa, and Fayoum districts are the most representative districts for Amaranth existence on the basis of edaphic resources. Itsa and Youssef Al Seddik, in addition to Itsa and Fayoum, resemble each other in species composition. High pH and CaCO₃ percentages were discriminatory in Ibshaway, Itsa, and Youssef Al Seddik. Ni was the cornerstone for districts partitioning in pruned trees. Finally, Amaranth was flourishing in both comfortable and harsh habitats with cultivated crops and orchards, as well as on the outskirts. The findings are considered to be valorized by decision makers in arable land management. Full article

The penetration rate of variable energy sources in the current power grid is increasing, with the aim being to expand the use of these energy sources and to replace the traditional black start power supply. This study investigates the black start of a photovoltaic storage joint system based on the system’s short-circuit ratio threshold. Firstly, the principles and control modes of the photovoltaic (PV) system, energy storage system (ESS), and high-voltage direct current (DC) transmission system are studied separately to build an overall model; secondly, computational determinations of the short-circuit ratio under different scenarios are introduced to analyze the strength of the system, and the virtual inertia and virtual damping of the PV system are configured based on this; finally, the change trend of the storage system’s state of charge (SOC) is computed and observed, and the limits of what the system can support in each stage are determined. An electromagnetic transient simulation model of a black start system is constructed in PSCAD/EMTDC, and according to the proposed recovery strategy, the system frequency is maintained in the range of 49.4~50.6 Hz during the entire black start process; the fluctuation in maximum frequency after the recovery of the DC transmission system is no more than 0.1%; and the fluctuation in photovoltaic power at each stage is less than 3%. In addition, all the key indexes meet the requirements for black start technology, which verifies the validity of the strategy and provides theoretical support and a practical reference for the black start of a grid with variable energy sources. Full article

Lychee (Lychee chinensis Sonn.) is a tropical fruit highly appreciated for its vivid red color, sweet flavor, and nutritional properties. However, it is highly perishable, with postharvest losses often due to oxidative browning and dehydration. This study evaluated the organic olive oil coating (OC), a natural lipidic system with the potential to act as a precursor for organogel development, combined with polyamide/polyethylene (PA/PE) packaging under passive modified atmosphere. Fruits were harvested at commercial maturity and divided into two groups: OC-treated and untreated control (CTR). Both groups were stored at 5 ± 1 °C and 90 ± 5% relative humidity and analyzed on days 0, 3, 6, and 9. The OC-treated fruits showed significantly better retention of physical, chemical, microbiological, and sensory qualities. The coating reduced oxidative stress and enzymatic browning, preserving color and firmness. The PA/PE packaging regulated gas exchange, lowering oxygen levels and delaying respiration and ripening. As a result, OC fruits had lower weight loss, a slower increase in browning index and maturity index, and better visual and sensory scores than the CTR group. This dual strategy proved effective in extending shelf life while maintaining the fruit’s appearance, flavor, and nutritional value. It represents a sustainable and natural approach to enhancing the postharvest stability of lychee. Full article

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder marked by amyloid-β (Aβ) plaques, neurofibrillary tangles, blood–brain barrier dysfunction, oxidative stress (OS), and neuroinflammation. Current treatments provide symptomatic relief, but do not halt the disease’s progression. OS plays a crucial role in AD pathogenesis by promoting Aβ accumulation. Nuclear factor erythroid 2-related factor 2 (NRF2) is a key regulator of the antioxidant response, influencing genes involved in OS mitigation, mitochondrial function, and inflammation. Dysregulation of NRF2 is implicated in AD, making it a promising therapeutic target. Emerging evidence suggests that adherence to a Mediterranean diet (MD), which is particularly rich in polyphenols from extra virgin olive oil (EVOO), is associated with improved cognitive function and a reduced risk of mild cognitive impairment. Polyphenols can activate NRF2, enhancing endogenous antioxidant defenses. This study employs a computational approach to explore the potential of bioactive compounds in EVOO to modulate NRF2-related pathways in AD. We analyzed transcriptomic data from AD and EVOO-treated samples to identify NRF2-associated genes, and used chemical structure-based analysis to compare EVOO’s bioactive compounds with known NRF2 activators. Enrichment analysis was performed to identify common biological functions between NRF2-, EVOO-, and AD-related pathways. Our findings highlight important factors and biological functions that provide new insight into the molecular mechanisms through which EVOO consumption might influence cellular pathways associated with AD via modulation of the NRF2 pathway. The presented approach provides a different perspective in the discovery of compounds that may contribute to neuroprotective mechanisms in the context of AD. Full article

As the core equipment for efficient wastewater treatment, the internal structure of microporous aeration bioreactors directly determines the mass transfer efficiency and treatment performance. Based on Computational Fluid Dynamics (CFD) technology, this study explores the optimization mechanism of a Spherical Grid-Structured on the internal flow field of the reactor through a 3D numerical simulation system, aiming to improve the aeration efficiency and resource utilization. This study used a combination of experimental and numerical simulations to compare and analyze different configurations of the Spherical Grid-Structure. The simulation results show that the optimal equilibrium of the flow field inside the reactor is achieved when the diameter of the grid sphere is 2980 mm: the average flow velocity is increased by 22%, the uniformity of the pressure distribution is improved by 25%, and the peak turbulent kinetic energy is increased by 30%. Based on the Kalman vortex street theory, the periodic vortex induced by the grid structure refines the bubble size to 50–80 microns, improves the oxygen transfer efficiency by 20%, increases the spatial distribution uniformity of bubbles by 35%, and significantly reduces the dead zone volume from 28% to 16.8%, which is a decrease of 40%. This study reveals the quantitative relationship between the structural parameters of the grid and the flow field characteristics through a pure numerical simulation, which provides a theoretical basis and quantifiable optimization scheme for the structural design of the microporous aeration bioreactor, which is of great significance in promoting the development of low-energy and high-efficiency wastewater treatment technology. Full article

Avian influenza A viruses (IAVs) pose a persistent threat to the poultry industry, causing substantial economic losses. Although traditional vaccines have helped reduce the disease burden, they typically rely on multivalent antigens, emphasize humoral immunity, and require intensive production. This study aimed to establish a genetically matched host–cell system to evaluate antigen-specific immune responses and identify conserved CD8+ T cell epitopes in avian influenza viruses. To this end, we developed an MHC class I genotype (B21)-matched host (Lohmann VALO SPF chicken) and cell vector (DF-1 cell line) model. DF-1 cells were engineered to express the hemagglutinin (HA) gene of clade 2.3.4.4b H5N1 either transiently or stably, and to stably express the matrix 1 (M1) and nucleoprotein (NP) genes of A/chicken/South Korea/SL20/2020 (H9N2, Y280-lineage). Following prime-boost immunization with HA-expressing DF-1 cells, only live cells induced strong hemagglutination inhibition (HI) and virus-neutralizing (VN) antibody titers in haplotype-matched chickens. Importantly, immunization with DF-1 cells transiently expressing NP induced stronger IFN-γ production than those expressing M1, demonstrating the platform’s potential for differentiating antigen-specific cellular responses. CD8+ T cell epitope mapping by mass spectrometry identified one distinct MHC class I-bound peptide from each of the HA-, M1-, and NP-expressing DF-1 cell lines. Notably, the identified HA epitope was conserved in 97.6% of H5-subtype IAVs, and the NP epitope in 98.5% of pan-subtype IAVs. These findings highlight the platform’s utility for antigen dissection and rational vaccine design. While limited by MHC compatibility, this approach enables identification of naturally presented epitopes and provides insight into conserved, functionally constrained viral targets. Full article

This study examines how the newly established United States pursued economic development through diplomatic and commercial initiatives with the Ottoman Empire, navigating regional powers and the era’s political-economic conditions. It analyzes using American archival sources how America endeavored to establish commercial and diplomatic relations with the Ottoman Empire in the Mediterranean and Black Sea regions, which it viewed as critical markets in the late 18th and early 19th centuries, before signing any formal agreement. The research tracks how these early efforts laid foundations for what would become one of the world’s largest economies. The study analyzes America’s diplomatic efforts to secure an agreement with the Ottoman Empire prior to the 7 May 1830 trade agreement—which laid the foundation for bilateral relations—alongside the reactions of regional powers, the prevailing conditions of the period, and the Ottoman administration’s reluctance due to various factors, based on U.S. archival sources that, to the best of our knowledge, have not previously been utilized in existing studies. Full article

The enteroendocrine system of the gastrointestinal (GI) tract is the largest endocrine organ in the human body, playing a central role in the regulation of hunger, satiety, digestion, and energy homeostasis. Numerous factors—including dietary components, physical activity, and the gut microbiota—affect the secretion of GI hormones. This study aims to analyze how these factors modulate enteroendocrine function and influence systemic metabolic regulation. This review synthesizes the current scientific literature on the physiology and distribution of enteroendocrine cells and mechanisms of hormone secretion in response to macronutrients, physical activity, and microbial metabolites. Special attention is given to the interactions between gut-derived signals and central nervous system pathways involved in appetite control. Different GI hormones are secreted in specific regions of the digestive tract in response to meal composition and timing. Macronutrients, particularly during absorption, stimulate hormone release, while physical activity influences hormone concentrations, decreasing ghrelin and increasing GLP-1, PYY, and leptin levels. The gut microbiota, through fermentation and metabolite production (e.g., SCFAs and bile acids), modulates enteroendocrine activity. Species such as Akkermansia muciniphila are associated with improved gut barrier integrity and enhanced GLP-1 secretion. These combined effects contribute to appetite regulation and energy balance. Diet composition, physical activity, and gut microbiota are key modulators of gastrointestinal hormone secretion. Their interplay significantly affects appetite regulation and metabolic health. A better understanding of these relationships may support the development of personalized strategies for managing obesity and related disorders. Full article

Aiming to resolve the problem of the poor peanut seed-filling effect under high-speed operation when developing high-speed peanut sowing with precision, a peanut precision seed-metering machine with an auxiliary air-suction seed-filling device was designed. Focusing on the force analysis of peanuts in the seed chamber, the peanut seed disturbance principle in the seed-metering machine for the blowing structure of an auxiliary air-suction seed-filling device was clarified. The seed-filling process was analyzed via DEM-CFD coupled simulation, and three factors affecting the seed-filling effect were identified, namely the seed-filling chamber ‘V’ angle γ, the bottom blow-air-hole cross-sectional area S, and the bottom blow-air-hole airflow velocity v_q, and the ranges of values of the three factors were determined. The Box–Behnken test was conducted using the seed-filling index and leakage index as the indexes. The results show that the seed-filling chamber ‘V’ angle γ is 56.59°, the bottom blowhole cross-sectional area S is 1088.4 mm², and the blowhole air velocity v_q is 12.11 m·s⁻¹. At this point, the peanut seed suction qualification index and leakage index are optimal, the seed suction qualification index is 96.33%, and the seed leakage index is 2.59%. At the same time, the field test shows that a sowing operation speed of 8–12 km·h⁻¹, a qualified index > 93%, and a leakage index < 4.5% are required to meet the agronomic requirements of peanut precision sowing. Full article

Background and aim: Advanced Therapy Medicinal Products (ATMPs) are innovative drugs based on genes, tissues, or cells that target rare and severe diseases. ATMPs have shown promising clinical outcomes but are associated with high costs, raising questions about cost-effectiveness. Hence, this systematic review aims to analyze the cost-effectiveness and cost-utility profiles of the European Medicines Agency-authorized ATMPs for treating rare diseases. Methods: A systematic review was conducted following PRISMA guidelines. Studies were identified by searching PubMed, Embase, Web of Science, and ProQuest scientific databases. Economic evaluations reporting incremental cost-effectiveness/utility ratios (ICERs/ICURs) for ATMPs were included. Costs were standardized to 2023 Euros, and a cost-effectiveness plane was constructed to evaluate the results against willingness-to-pay (WTP) thresholds of EUR 50,000, EUR 100,000, and EUR 150,000 per QALY, as part of a sensitivity analysis. Results: A total of 61 studies met the inclusion criteria. ATMPs for rare blood diseases, such as tisagenlecleucel and axicabtagene ciloleucel, were found to be cost-effective in a majority of studies, with incremental QALYs ranging from 1.5 to 10 per patient over lifetime horizon. Tisagenlecleucel demonstrated a positive cost-effectiveness profile in the treatment of acute lymphoblastic leukemia (58%), while axicabtagene ciloleucel showed a positive profile in the treatment of diffuse large B-cell lymphoma (85%). Onasemnogene abeparvovec for spinal muscular atrophy (SMA) showed uncertain cost-effectiveness results, and voretigene neparvovec for retinal diseases was not cost-effective in 40% of studies, with incremental QALYs around 1.3 and high costs exceeding the WTP threshold set. Conclusions: ATMPs in treating rare diseases show promising economic potential, but cost-effectiveness varies across indications. Policymakers must balance innovation with system sustainability, using refined models and the long-term impact on patient outcomes. Full article