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Keywords = agricultural growth

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27 pages, 19279 KiB  
Article
Smart Hydroponic Cultivation System for Lettuce (Lactuca sativa L.) Growth Under Different Nutrient Solution Concentrations in a Controlled Environment
by Raul Herrera-Arroyo, Juan Martínez-Nolasco, Enrique Botello-Álvarez, Víctor Sámano-Ortega, Coral Martínez-Nolasco and Cristal Moreno-Aguilera
Appl. Syst. Innov. 2025, 8(4), 110; https://doi.org/10.3390/asi8040110 (registering DOI) - 7 Aug 2025
Abstract
The inclusion of the Internet of Things (IoT) in indoor agricultural systems has become a fundamental tool for improving cultivation systems by providing key information for decision-making in pursuit of better performance. This article presents the design and implementation of an IoT-based agricultural [...] Read more.
The inclusion of the Internet of Things (IoT) in indoor agricultural systems has become a fundamental tool for improving cultivation systems by providing key information for decision-making in pursuit of better performance. This article presents the design and implementation of an IoT-based agricultural system installed in a plant growth chamber for hydroponic cultivation under controlled conditions. The growth chamber is equipped with sensors for air temperature, relative humidity (RH), carbon dioxide (CO2) and photosynthetically active photon flux, as well as control mechanisms such as humidifiers, full-spectrum Light Emitting Diode (LED) lamps, mini split air conditioner, pumps, a Wi-Fi surveillance camera, remote monitoring via a web application and three Nutrient Film Technique (NFT) hydroponic systems with a capacity of ten plants each. An ATmega2560 microcontroller manages the smart system using the MODBUS RS-485 communication protocol. To validate the proper functionality of the proposed system, a case study was conducted using lettuce crops, in which the impact of different nutrient solution concentrations (50%, 75% and 100%) on the phenotypic development and nutritional content of the plants was evaluated. The results obtained from the cultivation experiment, analyzed through analysis of variance (ANOVA), show that the treatment with 75% nutrient concentration provides an appropriate balance between resource use and nutritional quality, without affecting the chlorophyll content. This system represents a scalable and replicable alternative for protected agriculture. Full article
(This article belongs to the Special Issue Smart Sensors and Devices: Recent Advances and Applications Volume II)
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19 pages, 1717 KiB  
Article
A Multifaceted Approach to Optimizing Processed Tomato Production: Investigating the Combined Effects of Biostimulants and Reduced Nitrogen Fertilization
by Michela Farneselli, Lara Reale, Beatrice Falcinelli, Muhammad Zubair Akram, Stefano Cimarelli, Eleonore Cinti, Michela Paglialunga, Flavia Carbone, Euro Pannacci and Francesco Tei
Horticulturae 2025, 11(8), 931; https://doi.org/10.3390/horticulturae11080931 (registering DOI) - 7 Aug 2025
Abstract
Excessive nitrogen (N) fertilizer usage in agriculture has prompted the exploration of sustainable strategies to enhance nitrogen use efficiency (NUE) while maintaining crop yield and quality. Processed tomatoes (Solanum lycopersicum L.) were grown for two years (2023 and 2024) following a two-way [...] Read more.
Excessive nitrogen (N) fertilizer usage in agriculture has prompted the exploration of sustainable strategies to enhance nitrogen use efficiency (NUE) while maintaining crop yield and quality. Processed tomatoes (Solanum lycopersicum L.) were grown for two years (2023 and 2024) following a two-way factorial randomized complete block (RCBD) design, considering three biostimulants and three N regimes as two factors, to assess their morphophysiological, biochemical, anatomical and yield performances. Nitrogen application significantly influenced biomass accumulation, the leaf area index (LAI), nitrogen uptake and yield with notable comparable values between reduced and optimal nitrogen dose, indicating improved nitrogen use efficiency. Biostimulants showed limited effects alone but enhanced plant performance under reduced nitrogen conditions, particularly improving chlorophyll content, crop growth, N uptake, yield and anatomical adaptations. Moreover, compared to 2024, biostimulant application enhanced tomato growth more evidently in 2023 due to environmental variations, likely due to the occurrence of stress conditions. Importantly, biostimulants, together with N regimes, i.e., optimal and reduced doses, showed improved anatomical traits, especially regarding leaf thickness and thickness between the two epidermises, indicating adaptive responses that may support sustained productivity under N-limited conditions. Among the biostimulants used, the processed tomatoes responded better to protein hydrolysate and endophytic N-fixing bacteria than to seaweed extract. These findings suggest that although biostimulants alone were not affected, integrating them with reduced N fertilization provides a viable strategy for optimizing tomato production, conserving resources and minimizing the environmental impact without compromising yield or quality. Full article
(This article belongs to the Special Issue Effects of Biostimulants on Horticultural Crop Production)
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15 pages, 771 KiB  
Review
Trichoderma: Dual Roles in Biocontrol and Plant Growth Promotion
by Xiaoyan Chen, Yuntong Lu, Xing Liu, Yunying Gu and Fei Li
Microorganisms 2025, 13(8), 1840; https://doi.org/10.3390/microorganisms13081840 (registering DOI) - 7 Aug 2025
Abstract
The genus Trichoderma plays a pivotal role in sustainable agriculture through its multifaceted contributions to plant health and productivity. This review explores Trichoderma’s biological functions, including its roles as a biocontrol agent, plant growth promoter, and stress resilience enhancer. By producing various [...] Read more.
The genus Trichoderma plays a pivotal role in sustainable agriculture through its multifaceted contributions to plant health and productivity. This review explores Trichoderma’s biological functions, including its roles as a biocontrol agent, plant growth promoter, and stress resilience enhancer. By producing various enzymes, secondary metabolites, and volatile organic compounds, Trichoderma effectively suppresses plant pathogens, promotes root development, and primes plant immune responses. This review details the evolutionary adaptations of Trichoderma, which has transitioned from saprotrophism to mycoparasitism and established beneficial symbiotic relationships with plants. It also highlights the ecological versatility of Trichoderma in colonizing plant roots and improving soil health, while emphasizing its role in mitigating both biotic and abiotic stressors. With increasing recognition as a biostimulant and biocontrol agent, Trichoderma has become a key player in reducing chemical inputs and advancing eco-friendly farming practices. This review addresses challenges such as strain selection, formulation stability, and regulatory hurdles and concludes by advocating for continued research to optimize Trichoderma’s applications in addressing climate change, enhancing food security, and promoting a sustainable agricultural future. Full article
(This article belongs to the Special Issue Advances in Plant–Soil–Microbe Interactions)
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21 pages, 4581 KiB  
Article
Spatiotemporal Variations and Drivers of the Ecological Footprint of Water Resources in the Yangtze River Delta
by Aimin Chen, Lina Chang, Peng Zhao, Xianbin Sun, Guangsheng Zhang, Yuanping Li, Haojun Deng and Xiaoqin Wen
Water 2025, 17(15), 2340; https://doi.org/10.3390/w17152340 - 6 Aug 2025
Abstract
With the acceleration of urbanization in China, water resources have become a key factor restricting regional sustainable development. Current research primarily examines the temporal or spatial variations in the water resources ecological footprint (WREF), with limited emphasis on the integration of both spatial [...] Read more.
With the acceleration of urbanization in China, water resources have become a key factor restricting regional sustainable development. Current research primarily examines the temporal or spatial variations in the water resources ecological footprint (WREF), with limited emphasis on the integration of both spatial and temporal scales. In this study, we collected the data and information from the 2005–2022 Statistical Yearbook and Water Resources Bulletin of the Yangtze River Delta Urban Agglomeration (YRDUA), and calculated evaluation indicators: WREF, water resources ecological carrying capacity (WRECC), water resources ecological pressure (WREP), and water resources ecological surplus and deficit (WRESD). We primarily analyzed the temporal and spatial variation in the per capita WREF and used the method of Geodetector to explore factors driving its temporal and spatial variation in the YRDUA. The results showed that: (1) From 2005 to 2022, the per capita WREF (total water, agricultural water, and industrial water) of the YRDUA generally showed fluctuating declining trends, while the per capita WREF of domestic water and ecological water showed obvious growth. (2) The per capita WREF and the per capita WRECC were in the order of Jiangsu Province > Anhui Province > Shanghai City > Zhejiang Province. The spatial distribution of the per capita WREF was similar to those of the per capita WRECC, and most areas effectively consume water resources. (3) The explanatory power of the interaction between factors was greater than that of a single factor, indicating that the spatiotemporal variation in the per capita WREF of the YRDUA was affected by the combination of multiple factors and that there were regional differences in the major factors in the case of secondary metropolitan areas. (4) The per capita WREF of YRDUA was affected by natural resources, and the impact of the ecological condition on the per capita WREF increased gradually over time. The impact factors of secondary metropolitan areas also clearly changed over time. Our results showed that the ecological situation of per capita water resources in the YRDUA is generally good, with obvious spatial and temporal differences. Full article
(This article belongs to the Section Water Resources Management, Policy and Governance)
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20 pages, 1014 KiB  
Review
State of the Art on the Interaction of Entomopathogenic Nematodes and Plant Growth-Promoting Rhizobacteria to Innovate a Sustainable Plant Health Product
by Islam Ahmed Abdelalim Darwish, Daniel P. Martins, David Ryan and Thomais Kakouli-Duarte
Crops 2025, 5(4), 52; https://doi.org/10.3390/crops5040052 - 6 Aug 2025
Abstract
Insect pests cause severe damage and yield losses to many agricultural crops globally. The use of chemical pesticides on agricultural crops is not recommended because of their toxic effects on the environment and consumers. In addition, pesticide toxicity reduces soil fertility, poisons ground [...] Read more.
Insect pests cause severe damage and yield losses to many agricultural crops globally. The use of chemical pesticides on agricultural crops is not recommended because of their toxic effects on the environment and consumers. In addition, pesticide toxicity reduces soil fertility, poisons ground waters, and is hazardous to soil biota. Therefore, applications of entomopathogenic nematodes (EPNs) and plant growth-promoting rhizobacteria (PGPR) are an alternative, eco-friendly solution to chemical pesticides and mineral-based fertilizers to enhance plant health and promote sustainable food security. This review focuses on the biological and ecological aspects of these organisms while also highlighting the practical application of molecular communication approaches in developing a novel plant health product. This insight will support this innovative approach that combines PGPR and EPNs for sustainable crop production. Several studies have reported positive interactions between nematodes and bacteria. Although the combined presence of both organisms has been shown to promote plant growth, the molecular interactions between them are still under investigation. Integrating molecular communication studies in the development of a new product could help in understanding their relationships and, in turn, support the combination of these organisms into a single plant health product. Full article
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20 pages, 4173 KiB  
Article
Visual Observation of Polystyrene Microplastics/Nanoplastics in Peanut Seedlings and Their Effects on Growth and the Antioxidant Defense System
by Yuyang Li, Xinyi Huang, Qiang Lv, Zhanqiang Ma, Minhua Zhang, Jing Liu, Liying Fan, Xuejiao Yan, Nianyuan Jiao, Aneela Younas, Muhammad Shaaban, Jiakai Gao, Yanfang Wang and Ling Liu
Agronomy 2025, 15(8), 1895; https://doi.org/10.3390/agronomy15081895 - 6 Aug 2025
Abstract
Peanut cultivation is widely practiced using plastic mulch film, resulting in the accumulation of microplastics/nanoplastics (MPs/NPs) in agricultural soils, potentially negatively affecting peanut growth. To investigate the effects of two polystyrene (PS) sizes (5 μm, 50 nm) and three concentrations (0, 10, and [...] Read more.
Peanut cultivation is widely practiced using plastic mulch film, resulting in the accumulation of microplastics/nanoplastics (MPs/NPs) in agricultural soils, potentially negatively affecting peanut growth. To investigate the effects of two polystyrene (PS) sizes (5 μm, 50 nm) and three concentrations (0, 10, and 100 mg L−1) on peanut growth, photosynthetic efficiency, and physiological characteristics, a 15-day hydroponic experiment was conducted using peanut seedlings as the experimental material. The results indicated that PS-MPs/NPs inhibited peanut growth, reduced soil and plant analyzer development (SPAD) values (6.7%), and increased levels of malondialdehyde (MDA, 22.0%), superoxide anion (O2, 3.8%) superoxide dismutase (SOD, 16.1%) and catalase (CAT, 12.1%) activity, and ascorbic acid (ASA, 12.6%) and glutathione (GSH, 9.1%) contents compared to the control. Moreover, high concentrations (100 mg L−1) of PS-MPs/NPs reduced the peanut shoot fresh weight (16.1%) and SPAD value (7.2%) and increased levels of MDA (17.1%), O2 (5.6%), SOD (10.6%), POD (27.2%), CAT (7.3%), ASA (12.3%), and GSH (6.8%) compared to low concentrations (10 mg L−1) of PS-MPs/NPs. Notably, under the same concentration, the impact of 50 nm PS-NPs was stronger than that of 5 μm PS-MPs. The peanut shoot fresh weight of PS-NPs was lower than that of PS-MPs by an average of 7.9%. Additionally, we found that with an increasing exposure time of PS-MPs/NPs, the inhibitory effect of low concentrations of PS-MPs/NPs on the fresh weight was decreased by 2.5%/9.9% (5 d) and then increased by 7.7%/2.7% (15 d). Conversely, high concentrations of PS-MPs/NPs consistently reduced the fresh weight. Correlation analysis revealed a clear positive correlation between peanut biomass and both the SPAD values as well as Fv/Fm, and a negative correlation with MDA, SOD, CAT, ASA, and GSH. Furthermore, the presence of PS-MPs/NPs in roots, stems, and leaves was confirmed using a confocal laser scanning microscope. The internalization of PS-MPs/NPs within peanut tissues negatively impacted peanut growth by increasing the MDA and O2 levels, reducing the SPAD values, and inhibiting the photosynthetic capacity. In conclusion, the study demonstrated that the effects of PS on peanuts were correlated with the PS size, concentration, and exposure time, highlighting the potential risk of 50 nm to 5 μm PS being absorbed by peanuts. Full article
(This article belongs to the Collection Crop Physiology and Stress)
17 pages, 1097 KiB  
Review
Natural Feed Additives in Sub-Saharan Africa: A Systematic Review of Efficiency and Sustainability in Ruminant Production
by Zonaxolo Ntsongota, Olusegun Oyebade Ikusika and Thando Conference Mpendulo
Ruminants 2025, 5(3), 36; https://doi.org/10.3390/ruminants5030036 - 6 Aug 2025
Abstract
Ruminant livestock production plays a crucial role in the agricultural systems of Sub-Saharan Africa, significantly supporting rural livelihoods through income generation, improved nutrition, and employment opportunities. Despite its importance, the sector continues to face substantial challenges, such as low feed quality, seasonal feed [...] Read more.
Ruminant livestock production plays a crucial role in the agricultural systems of Sub-Saharan Africa, significantly supporting rural livelihoods through income generation, improved nutrition, and employment opportunities. Despite its importance, the sector continues to face substantial challenges, such as low feed quality, seasonal feed shortages, and climate-related stresses, all of which limit productivity and sustainability. Considering these challenges, the adoption of natural feed additives has emerged as a promising strategy to enhance animal performance, optimise nutrient utilisation, and mitigate environmental impacts, including the reduction of enteric methane emissions. This review underscores the significant potential of natural feed additives such as plant extracts, essential oils, probiotics, and mineral-based supplements such as fossil shell flour as sustainable alternatives to conventional growth promoters in ruminant production systems across the region. All available documented evidence on the topic from 2000 to 2024 was collated and synthesised through standardised methods of systematic review protocol—PRISMA. Out of 319 research papers downloaded, six were included and analysed directly or indirectly in this study. The results show that the addition of feed additives to ruminant diets in all the studies reviewed significantly (p < 0.05) improved growth parameters such as average daily growth (ADG), feed intake, and feed conversion ratio (FCR) compared to the control group. However, no significant (p > 0.05) effect was found on cold carcass weight (CCW), meat percentage, fat percentage, bone percentage, or intramuscular fat (IMF%) compared to the control. The available evidence indicates that these additives can provide tangible benefits, including improved growth performance, better feed efficiency, enhanced immune responses, and superior meat quality, while also supporting environmental sustainability by reducing nitrogen excretion and decreasing dependence on antimicrobial agents. Full article
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24 pages, 62899 KiB  
Essay
Monitoring and Historical Spatio-Temporal Analysis of Arable Land Non-Agriculturalization in Dachang County, Eastern China Based on Time-Series Remote Sensing Imagery
by Boyuan Li, Na Lin, Xian Zhang, Chun Wang, Kai Yang, Kai Ding and Bin Wang
Earth 2025, 6(3), 91; https://doi.org/10.3390/earth6030091 (registering DOI) - 6 Aug 2025
Abstract
The phenomenon of arable land non-agriculturalization has become increasingly severe, posing significant threats to the security of arable land resources and ecological sustainability. This study focuses on Dachang Hui Autonomous County in Langfang City, Hebei Province, a region located at the edge of [...] Read more.
The phenomenon of arable land non-agriculturalization has become increasingly severe, posing significant threats to the security of arable land resources and ecological sustainability. This study focuses on Dachang Hui Autonomous County in Langfang City, Hebei Province, a region located at the edge of the Beijing–Tianjin–Hebei metropolitan cluster. In recent years, the area has undergone accelerated urbanization and industrial transfer, resulting in drastic land use changes and a pronounced contradiction between arable land protection and the expansion of construction land. The study period is 2016–2023, which covers the key period of the Beijing–Tianjin–Hebei synergistic development strategy and the strengthening of the national arable land protection policy, and is able to comprehensively reflect the dynamic changes of arable land non-agriculturalization under the policy and urbanization process. Multi-temporal Sentinel-2 imagery was utilized to construct a multi-dimensional feature set, and machine learning classifiers were applied to identify arable land non-agriculturalization with optimized performance. GIS-based analysis and the geographic detector model were employed to reveal the spatio-temporal dynamics and driving mechanisms. The results demonstrate that the XGBoost model, optimized using Bayesian parameter tuning, achieved the highest classification accuracy (overall accuracy = 0.94) among the four classifiers, indicating its superior suitability for identifying arable land non-agriculturalization using multi-temporal remote sensing imagery. Spatio-temporal analysis revealed that non-agriculturalization expanded rapidly between 2016 and 2020, followed by a deceleration after 2020, exhibiting a pattern of “rapid growth–slowing down–partial regression”. Further analysis using the geographic detector revealed that socioeconomic factors are the primary drivers of arable land non-agriculturalization in Dachang Hui Autonomous County, while natural factors exerted relatively weaker effects. These findings provide technical support and scientific evidence for dynamic monitoring and policy formulation regarding arable land under urbanization, offering significant theoretical and practical implications. Full article
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20 pages, 11306 KiB  
Article
Bioactive Extracts of Spirulina platensis Inhibit Colletotrichum orchidearum and Fusarium nirenbergiae: A Green Approach to Hydroponic Lettuce Protection
by Leticia Eduarda Bender, Emily da Luz Monteiro, José Luís Trevizan Chiomento and Luciane Maria Colla
Processes 2025, 13(8), 2483; https://doi.org/10.3390/pr13082483 - 6 Aug 2025
Abstract
The growing demand for food and the environmental impact of conventional agriculture have prompted the search for sustainable alternatives. Phycocyanin (PC) and total phenolic compounds (TPC) extracted from Spirulina platensis have shown potential for the biological control of phytopathogens. The extraction method directly [...] Read more.
The growing demand for food and the environmental impact of conventional agriculture have prompted the search for sustainable alternatives. Phycocyanin (PC) and total phenolic compounds (TPC) extracted from Spirulina platensis have shown potential for the biological control of phytopathogens. The extraction method directly influences the yield and stability of these compounds. This study aimed to establish an efficient extraction protocol for PC and TPC and to evaluate their antimicrobial efficacy in vitro against Colletotrichum orchidearum, Fusarium nirenbergiae, and Alternaria sp. isolated from hydroponically grown lettuce. The phytopathogens were identified based on phylogenetic analyses using sequences from the ITS, EF1-α, GAPDH, and RPB2 gene regions. This is the first report of C. orchidearum in hydroponic lettuce culture in Brazil, expanding its known host range. Extracts were obtained using hydroalcoholic solvents and phosphate buffer (PB), combined with ultrasound-assisted extraction (bath and probe). The extracts were tested for in vitro antifungal activity. Data were analyzed by ANOVA (p < 0.05), followed by Tukey’s test. The combination of the PB and ultrasound probe resulted in the highest PC (95.6 mg·g−1 biomass) and TPC (21.9 mg GAE·g−1) yields, using 10% (w/v) biomass. After UV sterilization, the extract retained its PC and TPC content. The extract inhibited C. orchidearum by up to 53.52% after three days and F. nirenbergiae by 54.17% on the first day. However, it promoted the growth of Alternaria sp. These findings indicate that S. platensis extracts are a promising alternative for the biological control of C. orchidearum and F. nirenbergiae in hydroponic systems. Full article
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24 pages, 5022 KiB  
Article
Aging-Invariant Sheep Face Recognition Through Feature Decoupling
by Suhui Liu, Chuanzhong Xuan, Zhaohui Tang, Guangpu Wang, Xinyu Gao and Zhipan Wang
Animals 2025, 15(15), 2299; https://doi.org/10.3390/ani15152299 - 6 Aug 2025
Abstract
Precise recognition of individual ovine specimens plays a pivotal role in implementing smart agricultural platforms and optimizing herd management systems. With the development of deep learning technology, sheep face recognition provides an efficient and contactless solution for individual sheep identification. However, with the [...] Read more.
Precise recognition of individual ovine specimens plays a pivotal role in implementing smart agricultural platforms and optimizing herd management systems. With the development of deep learning technology, sheep face recognition provides an efficient and contactless solution for individual sheep identification. However, with the growth of sheep, their facial features keep changing, which poses challenges for existing sheep face recognition models to maintain accuracy across the dynamic changes in facial features over time, making it difficult to meet practical needs. To address this limitation, we propose the lifelong biometric learning of the sheep face network (LBL-SheepNet), a feature decoupling network designed for continuous adaptation to ovine facial changes, and constructed a dataset of 31,200 images from 55 sheep tracked monthly from 1 to 12 months of age. The LBL-SheepNet model addresses dynamic variations in facial features during sheep growth through a multi-module architectural framework. Firstly, a Squeeze-and-Excitation (SE) module enhances discriminative feature representation through adaptive channel-wise recalibration. Then, a nonlinear feature decoupling module employs a hybrid channel-batch attention mechanism to separate age-related features from identity-specific characteristics. Finally, a correlation analysis module utilizes adversarial learning to suppress age-biased feature interference, ensuring focus on age-invariant identifiers. Experimental results demonstrate that LBL-SheepNet achieves 95.5% identification accuracy and 95.3% average precision on the sheep face dataset. This study introduces a lifelong biometric learning (LBL) mechanism to mitigate recognition accuracy degradation caused by dynamic facial feature variations in growing sheep. By designing a feature decoupling network integrated with adversarial age-invariant learning, the proposed method addresses the performance limitations of existing models in long-term individual identification. Full article
(This article belongs to the Section Animal System and Management)
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17 pages, 54671 KiB  
Article
Pep-VGGNet: A Novel Transfer Learning Method for Pepper Leaf Disease Diagnosis
by Süleyman Çetinkaya and Amira Tandirovic Gursel
Appl. Sci. 2025, 15(15), 8690; https://doi.org/10.3390/app15158690 (registering DOI) - 6 Aug 2025
Abstract
The health of crops is a major challenge for productivity growth in agriculture, with plant diseases playing a key role in limiting crop yield. Identifying and understanding these diseases is crucial to preventing their spread. In particular, greenhouse pepper leaves are susceptible to [...] Read more.
The health of crops is a major challenge for productivity growth in agriculture, with plant diseases playing a key role in limiting crop yield. Identifying and understanding these diseases is crucial to preventing their spread. In particular, greenhouse pepper leaves are susceptible to diseases such as mildew, mites, caterpillars, aphids, and blight, which leave distinctive marks that can be used for disease classification. The study proposes a seven-class classifier for the rapid and accurate diagnosis of pepper diseases, with a primary focus on pre-processing techniques to enhance colour differentiation between green and yellow shades, thereby facilitating easier classification among the classes. A novel algorithm is introduced to improve image vibrancy, contrast, and colour properties. The diagnosis is performed using a modified VGG16Net model, which includes three additional layers for fine-tuning. After initialising on the ImageNet dataset, some layers are frozen to prevent redundant learning. The classification is additionally accelerated by introducing flattened, dense, and dropout layers. The proposed model is tested on a private dataset collected specifically for this study. Notably, this work is the first to focus on diagnosing aphid and caterpillar diseases in peppers. The model achieves an average accuracy of 92.00%, showing promising potential for seven-class deep learning-based disease diagnostics. Misclassifications in the aphid class are primarily due to the limited number of samples available. Full article
(This article belongs to the Section Computing and Artificial Intelligence)
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20 pages, 312 KiB  
Article
Pimelea and Its Toxicity: A Survey of Landholder Experiences and Management Practices
by Rashid Saleem, Shane Campbell, Mary T. Fletcher, Sundaravelpandian Kalaipandian and Steve W. Adkins
Toxins 2025, 17(8), 393; https://doi.org/10.3390/toxins17080393 - 6 Aug 2025
Abstract
Pimelea is one of the highly toxic plants in Australia, particularly affecting cattle. It contains simplexin, a potent toxin that can cause Pimelea poisoning (St. George Disease) in livestock. A survey was conducted to assess the current impact of Pimelea on livestock production, [...] Read more.
Pimelea is one of the highly toxic plants in Australia, particularly affecting cattle. It contains simplexin, a potent toxin that can cause Pimelea poisoning (St. George Disease) in livestock. A survey was conducted to assess the current impact of Pimelea on livestock production, pasture systems, and financial losses among agricultural producers. In addition, information was also sought about the environmental conditions that facilitate its growth and the effectiveness of existing management strategies. The survey responses were obtained from producers affected by Pimelea across nine different Local Government Areas, through three States, viz., Queensland, New South Wales, and South Australia. Pimelea was reported to significantly affect animal production, with 97% of producers surveyed acknowledging its detrimental effects. Among livestock, cattle were the most severely affected (94%), when compared to sheep (13%), goats (3%), and horses (3%). The presence of Pimelea was mostly observed in spring (65%) and winter (48%), although 29% of respondents indicated that it could be present all year-round under favorable rainfall conditions. Germination was associated with light to moderate rainfall (52%), while only 24% linked it to heavy rainfall. Pimelea simplex F. Muell. was the most frequently encountered species (71%), followed by Pimelea trichostachya Lindl. (26%). Infestations were reported to occur annually by 47% of producers, with 41% noting occurrences every 2 to 5 years. Financially, producers estimated average annual losses of AUD 67,000, with 50% reporting an average of 26 cattle deaths per year, reaching up to 105 deaths in severe years. Some producers were spending up to AUD 2100 per annum to manage Pimelea. While chemical and physical controls were commonly employed, integrating competitive pastures and alternative livestock, such as sheep and goats, was considered as a potential management strategy. This study reiterates the need for further research on sustainable pasture management practices to reduce Pimelea-related risks to livestock and agricultural production systems. Full article
(This article belongs to the Special Issue Plant Toxin Emergency)
26 pages, 1407 KiB  
Review
ZnO Nanoparticles: Advancing Agricultural Sustainability
by Lekkala Venkata Ravishankar, Nidhi Puranik, VijayaDurga V. V. Lekkala, Dakshayani Lomada, Madhava C. Reddy and Amit Kumar Maurya
Plants 2025, 14(15), 2430; https://doi.org/10.3390/plants14152430 - 5 Aug 2025
Abstract
Micronutrients play a prominent role in plant growth and development, and their bioavailability is a growing global concern. Zinc is one of the most important micronutrients in the plant life cycle, acting as a metallic cofactor for numerous biochemical reactions within plant cells. [...] Read more.
Micronutrients play a prominent role in plant growth and development, and their bioavailability is a growing global concern. Zinc is one of the most important micronutrients in the plant life cycle, acting as a metallic cofactor for numerous biochemical reactions within plant cells. Zinc deficiency in plants leads to various physiological abnormalities, ultimately affecting nutritional quality and posing challenges to food security. Biofortification methods have been adopted by agronomists to increase Zn concentrations in crops through optimal foliar and soil applications. Changing climatic conditions and conventional agricultural practices alter edaphic factors, reducing zinc bioavailability in soils due to abrupt weather changes. Precision agriculture emphasizes need-based and site-specific technologies to address these nutritional deficiencies. Nanoscience, a multidimensional approach, reduces particle size to the nanometer (nm) scale to enhance their efficiency in precise amounts. Nanoscale forms of Zn+2 and their broad applications across crops are gaining attention in agriculture under varied application methods. This review focuses on the significance of Zn oxide (ZnO) nanoparticles (ZnONPs) and their extensive application in crop production. We also discuss optimum dosage levels, ZnONPs synthesis, application methods, toxicity, and promising future strategies in this field. Full article
(This article belongs to the Special Issue Nanotechnology in Crop Physiology and Sustainable Agriculture)
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18 pages, 2376 KiB  
Article
Selection and Characterisation of Elite Mesorhizobium spp. Strains That Mitigate the Impact of Drought Stress on Chickpea
by María Camacho, Francesca Vaccaro, Pilar Brun, Francisco Javier Ollero, Francisco Pérez-Montaño, Miriam Negussu, Federico Martinelli, Alessio Mengoni, Dulce Nombre Rodriguez-Navarro and Camilla Fagorzi
Agriculture 2025, 15(15), 1694; https://doi.org/10.3390/agriculture15151694 - 5 Aug 2025
Abstract
The chickpea (Cicer arietinum L.) is a key legume crop in Mediterranean agriculture, valued for its nutritional profile and adaptability. However, its productivity is severely impacted by drought stress. To identify microbial solutions that enhance drought resilience, we isolated seven Mesorhizobium strains [...] Read more.
The chickpea (Cicer arietinum L.) is a key legume crop in Mediterranean agriculture, valued for its nutritional profile and adaptability. However, its productivity is severely impacted by drought stress. To identify microbial solutions that enhance drought resilience, we isolated seven Mesorhizobium strains from chickpea nodules collected in southern Spain and evaluated their cultivar-specific symbiotic performance. Two commercial cultivars (Pedrosillano and Blanco Lechoso) and twenty chickpea germplasms were tested under growth chamber and greenhouse conditions, both with and without drought stress. Initial screening in a sterile substrate using nodulation assays, shoot/root dry weight measurements, and acetylene reduction assays identified three elite strains (ISC11, ISC15, and ISC25) with superior symbiotic performance and nitrogenase activity. Greenhouse trials under reduced irrigation demonstrated that several strain–cultivar combinations significantly mitigated drought effects on plant biomass, with specific interactions (e.g., ISC25 with RR-98 or BT6-19) preserving over 70% of shoot biomass relative to controls. Whole-genome sequencing of the elite strains revealed diverse taxonomic affiliations—ISC11 as Mesorhizobium ciceri, ISC15 as Mesorhizobium mediterraneum, and ISC25 likely representing a novel species. Genome mining identified plant growth-promoting traits including ACC deaminase genes (in ISC11 and ISC25) and genes coding for auxin biosynthesis-related enzymes. Our findings highlight the potential of targeted rhizobial inoculants tailored to chickpea cultivars to improve crop performance under water-limiting conditions. Full article
(This article belongs to the Special Issue Beneficial Microbes for Sustainable Crop Production)
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21 pages, 12523 KiB  
Article
Essential Oils as an Antifungal Alternative for the Control of Various Species of Fungi Isolated from Musa paradisiaca: Part I
by Maritza D. Ruiz Medina and Jenny Ruales
Microorganisms 2025, 13(8), 1827; https://doi.org/10.3390/microorganisms13081827 - 5 Aug 2025
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Abstract
This study evaluated the antifungal potential of essential oils (EOs): oregano (Origanum vulgare), rosemary (Salvia rosmarinus), clove (Syzygium aromaticum), thyme (Thymus vulgaris), cinnamon (Cinnamomum verum), and basil (Ocimum basilicum). These oils [...] Read more.
This study evaluated the antifungal potential of essential oils (EOs): oregano (Origanum vulgare), rosemary (Salvia rosmarinus), clove (Syzygium aromaticum), thyme (Thymus vulgaris), cinnamon (Cinnamomum verum), and basil (Ocimum basilicum). These oils were tested against fungi isolated from banana peels (Musa paradisiaca). The fungi tested were identified through macroscopic and microscopic analyses and DNA sequencing, after being isolated in potato dextrose agar (PDA) medium modified with 0.05% chloramphenicol. Subsequently, the antifungal properties of the tested essential oils were evaluated in vitro at concentrations of 200, 400, 600, 800, and 1000 ppm prepared in a 0.05% Tween 80 solution. Cinnamon EOs showed the highest antifungal activity, significantly inhibiting the growth of pathogens at a concentration of 400 ppm. Other EOs showed moderate effects at higher concentrations: rosemary inhibited fungal growth at 600 ppm, oregano at 800 ppm, and clove at 1000 ppm. These findings highlight the potential of EOs as eco-friendly alternatives to synthetic fungicides, contributing to the development of sustainable agricultural practices and the post-harvest management of bananas. It is recommended to conduct future research to assess the economic viability and practical impacts of large-scale applications. Full article
(This article belongs to the Special Issue Current Pattern in Epidemiology and Antifungal Resistance)
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