Search Results (93)

This paper presents the identification of the functional requirements and development of a preliminary concept of the AgriRover, a low-cost, modular autonomous vehicle intended to support sustainable practices in traditional vineyards in developing countries, focusing on the Ica region of Peru. Viticulture in this region faces acute challenges such as soil salinity, climate variability, labour shortages, and low technological readiness. Rather than offering a ready-made technological integration, this study adopts a step-by-step design approach grounded in the realities of smallholder farmers. The authors mapped the phenological stages of grapevines using the BBCH scale and systematically reviewed available sensing and monitoring technologies to determine the most context-appropriate solutions. Virtual modelling and preliminary analysis validate AgriRover’s geometric configuration and path-following capabilities within narrow vineyard rows. The proposed platform is meant to be adaptable, scalable, and maintainable using locally available material and human resources. AgriRover offers a practical and affordable foundation for precision agriculture in resource-constrained settings by aligning viticultural challenges with sensor deployment strategies and sustainability criteria. The sustainability analysis of the initial AgriRover concept was evaluated using the CML methodology, accounting for local waste processing rates and energy mixes to reflect environmental realities in Peru. Full article

Background: Head and neck squamous cell carcinoma (HNSCC) exhibit considerable heterogeneity, complicating the prediction of disease progression and treatment response. Consequently, researchers are actively investigating reliable biomarkers to forecast disease trajectories and inform therapeutic decisions. This study examines the role of BAG3, a protein involved in cell survival and stress response, as a potential predictive marker in HNSCC. The objective is to analyze BAG3 expression across various HNSCC types and correlate it with disease-free survival (DFS), aiming to elucidate the influence of BAG3 positivity on cancer progression. Methods: A multi-institutional retrospective study was conducted by analyzing BAG3 expression by immunohistochemistry in 104 tissue samples from patients with head and neck squamous cell carcinoma (HNSCC). The data were then correlated with DFS to assess the impact of BAG3 positivity on prognosis. Results: Immunohistochemical analysis of primary tumor samples collected from therapy-naive patients showed that BAG3 positivity was widespread across different head and neck cancer sites, with no significant correlation to sex, smoking status, HPV infection, tumor location, grade, or TNM parameters. However, BAG3 high positive patients had shorter DFS (median 23.2 months) compared to BAG3-negative patients (median 31.3 months). Cox analysis revealed that BAG3 high expression by IHC was associated with a more than 3-fold increased risk of disease recurrence. Conclusions: This study is the first to explore BAG3 as a biomarker for HNSCC recurrence. While preliminary findings suggest a link between BAG3 positivity and increased recurrence risk, further research is needed to validate these results. Prospective studies could help establish BAG3’s prognostic value and potentially lead to more personalized treatment approaches for HNSCC. Full article

Vin Santo is a passito wine produced mainly in Tuscany. In the traditional production of Vin Santo, fermentation occurs naturally. Only a few reports have explored the microbial ecology of Vin Santo. Therefore, the present study aimed to investigate the microbial ecology and its impact on the fermentative kinetics in traditional processes of Vin Santo carried out in two different Tuscan wineries. Despite the different systems used for drying the grapes, both wineries showed similar microbial ecology. Non-Saccharomyces yeasts were the dominant microbial population during grape drying in different succession, even though in the end, the dominant species (at different percentages) in both were Metschnikowia pulcherrima, Kloeckera apiculata, and Starmerella bacillaris. The spontaneous fermentations were instead both dominated by Saccharomyces cerevisiae, however in different concentration throughout the process, leading to a different ethanol content—12% (v/v) and 10.8% (v/v) in winery A and B, respectively. In both wineries, acetic bacteria and moulds did not grow. Considering the intraspecific biodiversity of S. cerevisiae populations, the vinifications of both wineries displayed very similar biodiversity indices. No single strain of S. cerevisiae dominated the entire fermentation process. The analysis identified 30 distinct genetic patterns in the fermentations of winery A and 23 in the fermentations of winery B. The work provided an insight into the microbial communities and their metabolomic interactions during Vin Santo production which could improve the management and control of the process. Full article

This study explores the valorization of bread waste for poly-β-hydroxybutyrate (PHB) production through a combined dark fermentation (DF) and photofermentation (PF) process. DF, performed using Lactobacillus amylovorus DSM 20532, efficiently converted bread waste into a lactate- and acetate-rich substrate within 120 h. The resulting fermented bread broth (FBB) was enriched with essential nutrients by adding digestate from anaerobic digestion, replacing the need for chemical supplements. Six purple non-sulfur bacteria (PNSB) strains were screened for PHB production in the FBB. Cereibacter johrii Pisa7 demonstrated the highest PHB accumulation (50.73% w PHB/w cells), and biomass increase (+1.26 g L⁻¹) over 336 h, leading to its selection for scale-up. Scale-up experiments were conducted in a 5 L photobioreactor with LED lights optimized for PNSB growth. C. johrii Pisa7 accumulated PHB at 15.17% and 11.51% w PHB/w cells in two independent trials, corresponding to productivities of 2.03 and 0.89 mg PHB L⁻¹ h⁻¹. These results confirm the scalability of the process while maintaining competitive PHB yields. This study highlights the potential of bread waste as a low-cost carbon source for bioplastic production, contributing to a circular bioeconomy by converting food waste into sustainable materials. Full article

Cancer is the second leading cause of death in the world, with scientific evidence indicating that the enzymes aromatase and cyclooxygenase 2 are upregulated in several types of cancer. Over the past 30 years, natural compounds have played a crucial role in cancer chemotherapy, and to date, many phytocompounds have been reported to interact with these enzymes, inhibiting their activity. Notably, several phytocompounds found in Rosmarinus officinalis L., a medicinal plant native to the Mediterranean region and cultivated around the world, have shown the ability to interact with these enzymes. This review examines the role of the main compounds contained in Rosmarinus officinalis L. as potential anticancer agents acting on aromatase and cyclooxygenase-2. Full article

The current global context demands the development of new solutions that prioritize energy efficiency, time optimization, safety, and sustainability. Urban transportation is one of the sectors undergoing significant transformation. Pursuing new urban transportation solutions has become increasingly intense, involving research institutions and companies. Considering this context, this study focused on the optimization procedures for designing a new vehicle capable of vertical take-off for urban air mobility applications. This paper reports on the optimization process of a thruster deployment mechanism using statistical techniques. In particular, the authors tested the use of Design of Experiments (DOE) techniques for the optimal design of a structural component of a new vehicle for urban mobility purposes under development at the Applied Mechanics laboratory of the Department of Industrial Engineering of the University of Salerno. For this reason, it was decided that a parametric multibody model would be developed in the Simscape Multibody environment for structural optimization using designed experiment plans to “guide” the designer in the analysis phase and search for an optimal configuration using a minimum number of configurations. Finally, employing FEM analysis, the chosen configuration was validated. This study allowed us to test the use of DOE techniques to design new systems. It allowed us to evaluate different configurations, the static and dynamic behavior, the constraining reactions present in the joints, and the active forces and torques of the actuators, highlighting the correlation between factors that can guide the designer in identifying optimal solutions. Full article

The construction sector is currently undergoing a deep digital transformation resulting from the prioritization of emerging technologies, among which are digital twins. New goals and opportunities are appearing that minimize the impact on a building’s lifecycle, reduce economic, environmental, and extra-social costs, optimize energetic performance, decrease energy consumption and emissions, and enhance the durability and service life of buildings and their components. Among the research activities that have led to the development of a maintenance management model (MMM), this paper deals with the digital-twin approach, considering it instrumental to the innovative governance of the building environment from a lifecycle-based and sustainable perspective. It includes paying attention to efficiency in terms of resource use, energy consumption, and the energy performance of buildings, supporting decarbonization processes, and environmental vulnerability due to natural disasters, extreme weather, and seismic events. Its current implementation is presented here. In this scenario, the authors, operating at BIG srl, an academic spinoff of the Mediterranean University of Reggio Calabria, Italy, working together with the startup Sysdev, based in Torino, Italy, the company Berna Engineering srl, based in Reggio Calabria, Italy, and ACCA Software spa, based in Avellino, Italy, introduce the experimental application of the DT4SEM for safety and well-being in buildings, which is specifically oriented to seismic behavior monitoring. The proposal, while highlighting the innovative character of DT approaches, responds to the need for reliable data for increasingly effective forecasts and the control of the seismic behavior of buildings, facilitating informed decision-making for building management while also optimizing maintenance schedules. Full article

Genomic surveillance based on sequencing the entire genetic code of SARS-CoV-2 involves monitoring and studying genetic changes and variations in disease-causing organisms such as viruses and bacteria. By tracing the virus, it is possible to prevent epidemic spread in the community, ensuring a ‘precision public health’ strategy. A peptide-based design was applied to provide an efficacious strategy that is able to counteract any emerging viral variant of concern dynamically and promptly to affect the outcomes of a pandemic at an early stage while waiting for the production of the anti-variant-specific vaccine, which require longer times. The inhibition of the interaction between the receptor-binding domain (RBD) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and one of the cellular receptors (DPP4) that its receptors routinely bind to infect human cells is an intriguing therapeutic approach to prevent the virus from entering human cells. Among the other modalities developed for this purpose, peptides surely offer unique advantages, including ease of synthesis, serum stability, low immunogenicity and toxicity, and small production and distribution chain costs. Here, we obtained a potent new inhibitor based on the rearrangement of a previously identified peptide that has been rationally designed on a cell dipeptidyl peptidase 4 (DPP4) sequence, a ubiquitous membrane protein known to bind the RBD-SPIKE domain of the virus. This novel peptide (named DPP4-derived), conceived as an endogenous “drug”, is capable of targeting the latest tested variants with a high affinity, reducing the VSV* DG-Fluc pseudovirus Omicron’s infection capacity by up to 14%, as revealed by in vitro testing in human Calu-3 cells. Surface plasmon resonance (SPR) confirmed the binding affinity of the new DPP4-derived peptide with Omicron variant RBD. Full article

This paper addresses the phenomenon of sloshing and the issues that arise during liquid handling at visual inspection stations. The pharmaceutical industry, recently put under pressure by the pandemic, has long adopted modular solutions consisting mainly of robotic islands. This work focuses on a visual inspection island for glass vials and ampules called VRU. This machine uses robotic arms to optimize the inspection process and enables automated control of a wide range of products using image recognition techniques and AI algorithms. However, the handling of containers in the presence of liquids requires special precautions to avoid the occurrence of bubbles inside the fluid that can prevent the cameras from correctly capturing any defects present. The banal solution involves a drastic reduction in the speeds and accelerations to which the liquids are subjected. However, using appropriate techniques makes it possible to achieve performance values similar to those obtainable when manipulating solid materials. The developed algorithms were tested using multibody simulations in the Mathworks Simscape environment and then validated using a six-axis Fanuc robot. In this study, however, the analysis conducted aimed to determine the correlations between trajectories, laws of motion, and sloshing in containers handled at high speed in industrial applications. In this study a multibody model was developed using a CFD analysis. The container consisted of a glass vial for pharmaceutical uses containing a liquid inside. The results obtained from the CFD analysis allowed us to calibrate the multibody model for the next phase of optimization of the laws of motion to be followed by the manipulator. Full article

Diabetes mellitus (DM) is a chronic metabolic disease affecting over 500 million people worldwide, which leads to severe complications and to millions of deaths yearly. When therapeutic goals are not reached with diet, physical activity, or non-insulin drugs, starting/adding insulin treatment is recommended by international guidelines. A novel recombinant insulin is icodec, a once-weekly insulin that successfully completed phase III trials and that has recently obtained the marketing authorization approval from the European Medicines Agency. This narrative review aims to assess icodec pharmacological and clinical features concerning evidence on benefit–risk profile, as compared to other basal insulins, addressing the potential impact on patients’ unmet needs. Icodec is a full agonist, recombinant human insulin analogue characterized by an ultra-long half-life (196 h), enabling its use in once-weekly administration. Phase III randomized clinical trials involving more than 4000 diabetic patients, mostly type 2 DM, documented non-inferiority of icodec, as compared to currently available basal insulins, in terms of estimated mean reduction of glycated hemoglobin levels; a superiority of icodec, compared to control, was confirmed in insulin-naïve patients (ONWARDS 1, 3, and 5), and in patients previously treated with basal insulin (ONWARDS 2). Icodec safety profile was comparable to the currently available basal insulins. Once-weekly icodec has the potential to improve patients’ adherence, thus positively influencing patients’ treatment satisfaction as well as quality of life, especially in type 2 DM insulin-naïve patients. An improved adherence might positively influence glycemic target achievement, reduce overall healthcare costs and overcome some of the unmet patients’ needs. Icodec has the potential to emerge as a landmark achievement in the evolution of insulin therapy, with a positive impact also for the National Health Services and the whole society. Full article

Low-temperature treatments can be applied to grapes or must before alcoholic fermentation to enhance the wine’s sensory characteristics. Several studies have shown that such practices have a positive effect on the polyphenol profile of the wine, but only a few surveys have examined the effect of these treatments on the yeast microbiota of grapes and wine. Therefore, this study aimed to evaluate how cryoextraction (freezing the grape with liquid nitrogen) and cold pre-fermentative maceration (at 5 °C for 48 h) affect the Saccharomyces and non-Saccharomyces populations during the winemaking process of red grapes, cv Sangiovese, conducted at two temperatures (20 and 30 °C). This research analyzed the concentration of various yeast species, their fermentation abilities, and the resulting wine’s aromatic profile. The Principal Component Analysis performed on yeast concentrations during the fermentations of various wines did not group the experimental wines based on treatment. However, the same groupings were highlighted when the concentrations of the volatile compounds, quantified in the experimental wines, were processed using the same statistical approach. Therefore, cryoextraction and cold pre-fermentative maceration seem to contribute less to the aromatic profile than the yeasts involved in the fermentation process. Full article

Polyphenols, an important class of natural products, are widely distributed in plant-based foods. These compounds are endowed with several biological activities and exert protective effects in various physiopathological contexts, including cancer. We herein investigated novel potential mechanisms of action of polyphenols, focusing on the proteasome, which has emerged as an attractive therapeutic target in cancers such as multiple myeloma. We carried out a structure-based virtual screening study using the DrugBank database as a repository of FDA-approved polyphenolic molecules. Starting from 86 polyphenolic compounds, based on the theoretical binding affinity and the interactions established with key residues of the chymotrypsin binding site, we selected 2 promising candidates, namely Hesperidin and Diosmin. The further assessment of the biologic activity highlighted, for the first time, the capability of these two molecules to inhibit the β5-proteasome activity and to exert anti-tumor activity against proteasome inhibitor-sensitive or resistant multiple myeloma cell lines. Full article

Nowadays, acne vulgaris therapies are often unsuccessful. One of the responsible factors for the formation of comedones and inflammatory lesions could be the peroxidation of squalene, a hydrocarbon representing one of the major components of human sebum. This peroxidation is increased by solar irradiation. The purpose of this work was to set up an in vivo method for the extraction and quantification of squalene from acne skin and to correlate the results with biophysical skin parameters such as sebum amount, protein content and TEWL. Healthy volunteers were used as control. The results obtained demonstrated that acne-prone skin had a major quantity of squalene, and, in the stratum corneum area, its peroxide form is present. Moreover, Spearman’s rank correlation showed a positive correlation between sebum content and peroxide squalene and between porphyrin intensity and peroxide squalene. Full article

A cryogel is a cross-linked polymer network with different properties that are determined by its manufacturing technique. The formation of a cryogel occurs at low temperatures and results in a porous structure whose pore size is affected by thermal conditions. The adjustable pore sizes of cryogels make them attractive for diverse applications. In this study, the influence of the external operational temperature, which affects the cooling and freezing rates, on the production of cryogels with 2% w/w agarose is investigated. Moreover, a mathematical model is developed to simulate the cryogel production process and provide an initial estimate of the pore size within the structure. The predictions of the model, supported by qualitative light microscopy images, demonstrate that cryogels produced at higher process temperatures exhibit larger pore sizes. Moreover, the existence of pore size distribution within the gel structure is confirmed. Finally, stress relaxation tests, coupled with an image analysis, validates that cryogels produced at lower temperatures possess a higher stiffness and slower water release rates. Full article

Recent interest in the special beer category has encouraged the search for novel brewing materials, including new ingredients and novel yeast strains, in order to differentiate the finished products. The aim of this work was to select non-brewing S. cerevisiae strains for the production of a fruit beer with raspberry. The in vitro tests and the wort fermentations allowed the selection of two sourdough S. cerevisiae strains, showing high maltose and maltotriose consumption, high ethanol production, and high viability. Fruit beers (FB) and control beers (CB) without raspberries were prepared. Fruit addition accelerated sugar consumption (7 days compared to 13 days) and increased ethanol and glycerol production by yeasts. Raspberry addition and the inoculated yeast strongly affected the aroma profile of beers. FB samples showed a higher amount of volatile organic compounds (VOCs); the most represented classes were alcohols, followed by esters and acids. FB inoculated by the selected S. cerevisiae SD12 showed the highest VOCs concentration (507.33 mg/L). Results highlighted the possible application of sourdough yeast strains for the brewing process, which, combined with raspberry addition, can be exploited for the production of beers with enhanced aromatic features and suitable chemical properties. Full article