Search Results (8,789)

The Eurasian lynx (Lynx lynx) is an apex predator and an important sentinel for trophically transmitted helminths acquired via predation on wild ungulates. On 2 March 2022, an adult male lynx that was road-killed in the Apuseni Mountains (Surducel hunting ground, Bihor County) was collected, frozen for biosafety, and a necropsy was performed. Taeniid cestodes were detected, with a total intestinal burden of nine adult specimens. Genetic analyses confirmed Taenia lynciscapreoli, and the obtained sequences were deposited in GenBank (PV843597, PV855065, PV844409). Phylogenetic inference based on cox1 assigned the Romanian isolate within the European cluster, distinct from the Chinese isolate, while showing genetic proximity to Taenia sp. (MW846305) that have been reported from a lynx in China. This study represents the first molecular identification of T. lynciscapreoli in the Eurasian lynx in Romania and, to our knowledge, the first record from Southeastern Europe. Full article

Estimating forest aboveground biomass (AGB) in mountainous forest ecosystems remains a significant challenge due to complex terrain, the high cost and limited applicability of traditional field-based methods. To address this issue, a remote sensing-based AGB estimation framework integrating intelligent optimization and machine learning was developed for Mount Tai in eastern China. Sentinel-2 multispectral data were selected to derive 105 candidate variables, including spectral bands, vegetation indices, texture features, and topographic factors, from which 17 key variables were selected using Pearson correlation analysis for model construction. A Support Vector Machine (SVM) optimized by the Pigeon-inspired optimization (PIO) algorithm was developed to adaptively determine optimal hyperparameters, and its performance was compared with that of Random Forest (RF) and standard SVM models. Among the three models, PIO-SVM produced the highest numerical accuracy. For the training dataset, it obtained an R² of 0.85 and an RMSE of 46.12 t/hm². For the testing dataset, it achieved an R² of 0.73 and an RMSE of 62.19 t/hm², compared with 0.72 and 66.25 t/hm² for the standard SVM model and 0.70 and 65.19 t/hm² for the RF model. The spatial distribution of AGB derived from the optimal model shows higher AGB values in the central and northern regions characterized by dense forest cover, in close agreement with field observations. Overall, the results suggest that PIO-based parameter optimization can improve SVM performance for AGB estimation in mountainous forests. This study provides a reliable and efficient framework for regional-scale monitoring of forest biomass and carbon sink dynamics. Full article

This study presents the development and implementation of a web-based geospatial platform for the quantitative assessment of land use and land cover change (LULCC) based on multispectral satellite images. The system operationalizes the Rao spectral diversity metric (Rao’s Q) to detect and quantify LULCC resulting from different environmental agents. The platform supports single-band (classic mode) or multi-band (multidimensional mode) processing. Its main functionalities include the interactive de-limitation of areas of interest (AOI) and calendar-based temporal selection, allowing analyses to be performed at discrete time points or at defined intervals. Among the tools available in the application are the automated calculation of Rao’s Q surfaces and maps of change between pairs of dates. Additionally, the platform allows the selection of several spectral indices, with the aim of supporting ecosystem monitoring and the characterization of the Earth’s surface. In the use case demonstration (Reykjanes Peninsula volcanic eruption of February 2024), the Rao’s Q method applied to Sentinel-2 SWIR imagery demonstrated strong performance in lava flow detection, with the multidimensional approach (bands 11 + 12) achieving the most balanced results (OA = 83.0%, PA = 84.0%, UA = 82.4%), while band 11 alone yielded the highest precision (UA = 97.4%). By integrating spatiotemporal analysis, spectral diversity metrics, and spectral indices into an accessible and extensible framework, the platform constitutes a robust tool for monitoring LULCC and assessing environmental impacts. Full article

Antimicrobial resistance (AMR) is a growing global concern, exacerbated by the overuse of antibiotics in livestock farming. Honey bees (Apis mellifera), widely used as bioindicators of environmental contamination, may also serve as sentinels for monitoring the environmental spread of antibiotic resistance genes (ARGs). This study investigated the presence of ARGs and the gut microbiota composition of honey bees sampled from 11 apiaries located in a region of Northwestern Italy characterized by intensive livestock farming. PCR and Sanger sequencing analyses revealed a widespread presence of tetracycline resistance genes—particularly tetB and tetC—as well as occasional detection of blaTEM, qnrB, and int1 genes. tetB and tetC were also identified in three bacterial colonies isolated from bee guts, notably in Hafnia spp. 16S rRNA gene sequencing of the gut microbiota revealed dominance of genera such as Bartonella, Snodgrassella, Gilliamella, Bombilactobacillus, and Lactobacillus. Some samples showed shifts in the microbial diversity. The findings confirm the potential of honey bees as bioindicators for environmental AMR surveillance and underscore the need for further research to elucidate correlations between ARG presence and microbial community structure in honey bees from various ecological contexts. Full article

Accurate crop classification in complex and heterogeneous agricultural landscapes is often challenged by mixed-pixel effects and spatial autocorrelation. This study proposes a prior-guided crop classification framework that integrates accessible Moderate Resolution Imaging Spectroradiometer (MODIS) optical and Sentinel-1 synthetic aperture radar (SAR) time-series data with explicit phenological and structural priors. By embedding physically meaningful constraints into temporal feature learning, the model shifts from purely data-driven learning toward biophysically interpretable discrimination between crop types and background classes. Performance was rigorously evaluated using spatial cross-validation (SCV) to ensure geographic independence. Results demonstrate that the prior-guided CNN achieves an overall accuracy (OA) of 98.66% and a Kappa of 0.9832, outperforming unguided deep learning and conventional machine learning models. Notably, the framework exhibits high spatial robustness, with a minimal performance gap between random and spatial validation (ΔOA = 0.0049). In addition to improving classification accuracy, integrating phenological features with SAR-based prior information enhances the stability of non-crop categories in fragmented scenarios, while leveraging readily available medium-resolution data to support large-scale applications. These findings demonstrate that embedding physically meaningful prior knowledge into multi-source time-series learning improves classification accuracy while enhancing spatial generalizability and interpretability. More broadly, the proposed framework offers a transferable paradigm for integrating domain knowledge with deep learning, providing a practical and scalable solution for crop mapping in heterogeneous agricultural landscapes using widely accessible medium-resolution data. Full article

Accurately monitoring the surface stabilization of waste dumps in open-pit coal mines is critical for hazard prevention and ecological reclamation. In arid and semi-arid regions, traditional optical remote sensing vegetation indices suffer from a systematic “response lag” in assessing physical stability due to the slow establishment of pioneer vegetation. To overcome this biological limitation, this study proposes a quantitative spatiotemporal monitoring framework based on time-series Interferometric Synthetic Aperture Radar (InSAR) coherence to detect early-stage geotechnical stabilization. Using Sentinel-1 imagery of the Balongtu coal mine, a sliding-window detection algorithm was developed to capture the physical transition of surface electromagnetic scattering mechanisms from active disturbance to stable consolidation. The main findings are as follows: (1) Statistical analysis identified a critical geophysical coherence threshold of 0.15, which effectively and objectively distinguishes active dumping disturbance zones from structurally stable areas. (2) The spatiotemporal evolution dynamics of the completed dump areas from 2017 to 2023 were successfully characterized, revealing that 87.6% of the open-pit areas achieved physical stabilization within three years post-mining, with a spatial distribution highly consistent with the objective operational rule of “mining first, dumping later”. (3) Accuracy assessment using 700 spatiotemporally balanced validation points—derived through strict visual interpretation of high-resolution optical imagery—demonstrated high algorithm reliability, achieving overall accuracies (OA) of 87.57% and 90.43% at half-yearly and annual monitoring intervals, respectively. By decoupling physical surface stabilization from optical greenness, this study provides a timely abiotic precursor indicator, offering scientific, quantitative decision support for precision ecological zoning and accelerated land turnover approval in mining areas. Full article

Leaf Area Index is a crucial biophysical variable, and its accurate estimation is essential for understanding vegetation dynamics. However, cloud cover significantly restricts optical remote sensing, hindering the generation of spatially continuous Leaf Area Index products. Remote sensing foundation models offer novel solutions to this challenge. This study presents an end-to-end framework based on the fine-tuned Prithvi foundation model for direct LAI retrieval from cloud-contaminated 30 m Harmonized Landsat and Sentinel-2 imagery. By mapping inputs directly to Hi-GLASS reference labels, the proposed architecture processes cloud contamination and vegetation signals simultaneously and circumvents the error propagation inherent in cascaded retrieval pipelines. Results demonstrate that the end-to-end LAI retrieval model significantly outperforms cascaded variants, achieving a superior R² (0.78) and lower RMSE (0.57). Furthermore, predictive accuracy exhibits a distinct U-shaped trajectory relative to the temporal mean cloud fraction, reaching an inflection point at 50–60% occlusion, which highlights the model’s implicit regularization capacity under severe atmospheric interference. This work establishes that direct feature learning with foundation models offers a more robust and streamlined pathway for generating continuous biophysical products from imperfect optical observations, prioritizing quantitative fidelity over artificial perceptual sharpness. Full article

Mast cells are heterogeneous, tissue-resident immune sentinels best known for their roles in allergy and peripheral inflammation. The discovery of mast cells within the meninges and brain parenchyma over a century ago raised enduring questions regarding their function in the central nervous system (CNS), their ontogeny, and distinction from peripheral counterparts. Brain mast cells are sparse and predominantly located in perivascular niches rather than forming dense aggregates, a feature that has made them difficult to study. Nevertheless, accumulating evidence implicates mast cells in diverse aspects of CNS physiology and pathology, including regulation of blood–brain barrier permeability and neurovascular function, as well as immune surveillance in contexts of infection and injury. The ability of mast cells to communicate with neighboring glial and neuronal networks suggests potential roles in modulating neural activity, development, and behavior, although this dimension remains incompletely understood. Much of the foundational literature predates advanced immunological tools, contributing to persistent misconceptions regarding the identity and significance of brain mast cells. In this review, we outline the history of research investigating this enigmatic aspect of mast cell biology, clarifying what is known, what remains speculative, and how emerging insights may help redefine the boundaries between classical immunology and neuroscience. Full article

Monitoring surface deformation at reclaimed airports under construction is crucial for ensuring construction safety. However, significant variations in surface scattering characteristics cause severe decorrelation, limiting the effectiveness of conventional single-polarization Interferometric Synthetic Aperture Radar (InSAR). To address the issue of insufficient coherent pixels, we propose a dual-polarization sequential InSAR technique and compare its performance with traditional Persistent Scatterer Interferometry (PSI) and Distributed Scatterer Interferometry (DSI) at the Dalian Jinzhou Bay International Airport (DJBIA). Using 89 Sentinel-1A dual-polarization (VV-VH) images (August 2022 to October 2025), the results demonstrate that VV and VH polarizations exhibit significant spatial complementarity, highlighting the necessity of multi-polarization data. Further, to address the issue of long-term changes in scattering characteristics, we applied the Sequential Estimation and Total Power-Enhanced Expectation Maximization Inversion (SETP-EMI) method, which dynamically integrates dual-polarization information and performs adaptive phase optimization. This approach significantly enhances monitoring capability in low-coherence areas of the airport under construction, effectively suppressing phase noise, improving interferogram quality, and yielding a more complete and reliable deformation field. Overall, this study systematically validates the SETP-EMI method with dual-polarization information for deformation monitoring at reclaimed airports under construction, providing technical support for engineering safety control and research on reclamation subsidence mechanisms. Full article

Milltailings deposition areas in abandoned mines are inherently unstable and spatially extensive and heterogeneous, making regional-scale field investigations challenging under intense rainfall. With the advancement of space–airborne remote sensing technologies, large-scale surface deformation monitoring has become feasible. In this study, a 22.02 km² abandoned mine in Lingqiu County, Shanxi Province, was selected as a case site; during the late-July 2023 extreme rainfall event, the site experienced large-scale surface displacements. Surface deformation was interpreted using Sentinel-1 SBAS-InSAR data, combined with differential digital elevation models (DEMs) derived from UAV surveys before and after heavy rainfall. A bivariate spatial autocorrelation analysis was conducted to evaluate the spatial relationship between differential DEMs and InSAR-derived deformation. The results indicate that: (1) SBAS-InSAR revealed significant spatial heterogeneity of ground deformation, with pronounced subsidence observed in the milltailings deposits; (2) the bivariate spatial autocorrelation analysis yielded a Moran’s I value of 0.2, suggesting a weak but positive spatial correlation between the DEM differences and InSAR results, with dispersed correlation patterns; (3) hotspot analysis highlighted notable clustering of deformation, with approximately 27.84% of the study area showing strong deformation responses, while 25.81% represented low–low clusters with limited deformation. Beyond tailings-deposit settings, this workflow is also applicable to the regional investigation of rainfall-responsive deformation and debris-flow-related terrain change on natural slopes under global change, providing technical support for surface investigations and offering insights for disaster early warning and ecological restoration in similar regions. Full article

Accurate reservoir dam detection in arid regions is challenging because of spectral similarity between dams and surrounding backgrounds, indistinct boundaries, and substantial target-scale variation. To address these issues, this study proposes a deep learning object detection with multi-conditional constraint assistance (DLOD&MCCA) framework that combines a dual deep enhancement YOLO network (DDE-YOLO) with a multi-conditional constraint assistance (MCCA) strategy. In DDE-YOLO, visible (VIS) and near-infrared (NIR) imagery are fused to enhance cross-spectral discrimination, while task-oriented architectural refinements improve the representation of dam targets with diverse scales and structural characteristics. Meanwhile, the MCCA strategy constrains the search space to geographically plausible candidate regions, thereby reducing background interference and improving detection efficiency. Experiments conducted on the self-constructed S2-Dam dataset and the public DIOR dataset show that DDE-YOLO achieves mAP50 values of 92.8% and 76.2%, respectively, outperforming existing state-of-the-art (SOTA) methods. Furthermore, regional-scale dam mapping in Xinjiang achieved an accuracy of over 95%, demonstrating the effectiveness and practical applicability of the proposed framework for large-scale reservoir dam detection in arid environments. Full article

Land subsidence (LS) is a widespread geoenvironmental problem driven by both natural processes and human activities. Identifying the main factors controlling LS susceptibility and their spatial contribution patterns is essential for LS management and mitigation. In this study, an interpretable earth observation framework was developed for the North China Plain (NCP) to quantify both spatial and non-spatial contributions of dominant LS drivers. Land displacement was derived from Sentinel-1A SAR images using Multi-Temporal Interferometric Synthetic Aperture Radar (MT-InSAR) processing. The displacement map was then combined with nine geoenvironmental variables to construct an LS susceptibility model using the eXtreme Gradient-Boosting (XGBoost) algorithm. The model performed well, with an R² of 0.96, an EVS of 0.96, and an MAE of 2.25 mm/yr. SHapley Additive exPlanations (SHAP) analysis was employed to quantify feature contributions and their effects on LS susceptibility. The results show that a deep groundwater level (DGL) was the dominant factor, followed by elevation and a shallow groundwater level (SGL). The effect of DGL was strongest when it ranged from −75 to 20 m. Elevation showed a clear effect on LS occurrence when values fall between 30 and 50 m. Relatively high subsidence sensitivity was mainly observed in areas where SGL was below −7 m. Interaction effects, particularly those between DGL and elevation and between DGL and SGL, further increased LS susceptibility in specific areas. The highest predicted susceptibility occurred in areas with DGL below −20 m and elevations below 30 m. Higher susceptibility was also identified where DGL was high and SGL ranged between −20 and −10 m, and where DGL was low and SGL ranged from 15 to 20 m. In contrast, factors such as slope and aspect had limited influence at the regional scale. The contributions of the predominant factors show obvious marginal effects and significant spatial heterogeneity to LS susceptibility. The results clarify where and how key factors shape subsidence and can inform targeted mitigation measures and urban planning by local authorities. Full article

Accurate estimation of crop development, water use and yield is essential for improving irrigation management in Mediterranean agricultural systems under increasing climate variability. However, many crop models require extensive input data and technical expertise, limiting their operational use by farmers and technicians. This study proposes an integrated agro-modelling framework that combines thermal time modelling, satellite-derived vegetation indices and simplified yield estimation approaches to assess maize phenology, crop water use and productivity under real farming conditions. A key component of the framework is the use of the Sentinel-2 Normalized Difference Vegetation Index (NDVI) time series to dynamically identify crop growth stages and derive actual basal crop coefficients (K_{cb act}), enabling the estimation of actual crop transpiration (T_{c act}). These NDVI-based estimates of actual K_cb and T_c were evaluated against simulations from the previously calibrated soil water balance model SIMDualKc. The results showed that the temporal profiles of the NDVI successfully captured the progression of the maize growth stages, although some discrepancies were observed during early stages of development due to the effects of the soil background and the satellite revisit intervals. An empirical relationship between the NDVI and K_cb was developed using multi-year observations and model simulations, improving crop transpiration estimation under field conditions. The NDVI-based approach adequately reproduced daily transpiration dynamics with good agreement with SIMDualKc simulations, yielding RMSE values of 0.11–0.69 mm d⁻¹ and errors generally below 21% of the mean transpiration rate. Seasonal transpiration estimates showed stronger agreement once canopy cover reached its maximum. The integrated AEZ–Stewart modelling framework incorporating NDVI-based transpiration estimations provided accurate yield predictions, with RMSE values of 1.7–2.3 t ha⁻¹ (representing less than 14% of the observed yields). Overall, the proposed framework demonstrates strong potential as a practical and scalable decision-support tool for irrigation management and yield assessment in Mediterranean maize systems. Its novelty lies in the operational integration of NDVI-derived crop development and transpiration estimates within a simplified yield modelling structure, offering a transferable approach applicable to other regions and cropping systems where satellite data are available. Full article

Urban cities have been intensely prone to floods during extreme rainfall events and water scarcity issues during dry periods in recent years. In this context, identifying rainwater harvesting potential (RWHP) regions in urban environments provides a sustainable approach to mitigate both urban flooding and water security, thereby improving urban stormwater management. Geospatial mapping of RWHP has tried to consider various hydrometeorological, topographical and other geospatial datasets, but integrating socio-economic factors over urban environments has not been explored much. The present study integrated remote sensing and hydrological-based information, such as slope, soil type, drainage density, geomorphology, topographic wetness index (TWI), land use land cover (LULC), rainfall, runoff coefficient, proximity to roads, and proximity to settlements for geospatial mapping of RWH potential zones for Hyderabad city using multi-criteria decision analysis (MCDA) and weighted overlay analysis (WOA). The resulting RWH potential map indicates that 80.20% of the area falls within the “low” potential category, 17.53% as “moderate”, 2.0% as “very low”, and only 0.25% as “high” potential, mainly in the southeastern portion near the Hussain Sagar outlet. These categories are spatially verified using Sentinel-2 LULC and Google Earth imagery to assess the qualitative plausibility of the mapped RWH potential zones. Northwestern areas, with loamy soils and mild slopes, demonstrate suitability for rooftop collection and percolation structures, highlighting the effectiveness of the proposed modelling framework for sustainable stormwater management for urban environments. Full article

Traditional satellite-derived bathymetry (SDB) often suffers from systematic optical path distortions due to the neglect of seafloor slope effects, leading to significant accuracy degradation in high-gradient coastal areas. This study proposes a Slope-Aware Physics-Informed Neural Network (SA-PINN) framework that synergistically utilizes ICESat-2 bathymetric photons and Sentinel-2 multispectral imagery. The core innovation involves a slope-aware operator, integrated into the radiative transfer-based physics loss function, which explicitly rectifies directional optical path deviations induced by seafloor inclination. By fusing physical mechanisms with data-driven features, the model utilizes a seven-dimensional feature space comprising four spectral bands, two directional slope components, and prior depth. Applications at Culebra, Maui, and Molokai demonstrate that SA-PINN significantly outperforms the Stumpf model, Random Forest, and standard CNNs, achieving root mean square errors (RMSE) of 1.36 m, 2.91 m, and 1.34 m, respectively. Ablation studies confirm that SA-PINN reduces RMSE by up to 37% compared to CNN in complex regions with slopes exceeding 10°, ensuring superior physical consistency and spatial continuity. This research provides a robust, in situ-free automated solution for high-resolution bathymetric mapping in remote and steep coastal environments globally. Full article