Search Results (342)

Background: Human activity is recognised as a major contributor to changes in Earth’s climate, land surface, oceans, ecosystems, and biodiversity. These alterations are largely due to greenhouse gas emissions, deforestation, mass pollution, and land degradation. In light of these environmental challenges, examining the psychological determinants of pro-environmental behaviour has become increasingly important. Study’s Aim: To provide a comprehensive model evaluating the structural relationships among biospheric values, green self-identity, green self-efficacy, and eco-anxiety to investigate the underlying mechanisms relating to the adoption of various pro-environmental behaviours (PEBs). Methods: An online self-report questionnaire was completed by 510 Italian participants (aged 18–55, M = 35.18, SD = 12.58) between November and December 2023. Data analysis was performed using R statistical software, employing Structural Equation Modelling. Results: The results indicate that eco-anxiety, green self-efficacy, and green self-identity are significant positive predictors of PEBs. Furthermore, green self-identity significantly influences eco-anxiety and green self-efficacy, while biospheric values are a major trigger for both green self-efficacy and green self-identity, but not for eco-anxiety. Conclusions: These findings suggest that while eco-anxiety can be an adaptive motivator for PEBs, biospheric values foster a green self-identity and self-efficacy, which in turn drive pro-environmental actions. The study concludes that encouraging biospheric values and strong green self-identity is crucial for promoting sustainable behaviours. Full article

Pangasius (Pangasianodon hypophthalmus) minced muscle with 1 and 2% salt was treated with different high-pressure processing and thermal methods, including conventional heat-induced gels (HIGs), high-pressure processing (HPP) prior to cooking (PC), HPP prior to setting (PS), and setting prior to HPP (SP), to evaluate for their effects on the selected physicochemical properties. The results showed that the PC treatment produced gels with a significantly higher gel strength (496.72–501.26 N·mm), hardness (9.62–10.14 N), and water-holding capacity (87.79–89.74%) compared to the HIG treatment, which showed a gel strength of 391.24 N·mm, a hardness of 7.36 N, and a water-holding capacity of 77.98%. PC gels also exhibited the typical microstructure of pressure-induced gels, with a denser and homogeneous microstructure compared to the rough and loosely connected structure of HIGs. In contrast, SP treatment exhibited the poorest gel quality in all parameters, with gel strength ranging from 319.79 to 338.34 N·mm, hardness from 5.87 to 6.31 N, and WHC from 71.91 to 73.72%. Meanwhile, the PS treatment showed a comparable gel quality to HIGs. SDS-PAGE analysis revealed protein degradation and aggregation in HPP-treated samples, with a decrease in the intensity of myosin heavy chains and actin bands. Fourier-transform infrared spectroscopy (FTIR) analysis showed minor shifts in protein secondary structures, with the PC treatment showing a significant increase in α-helices (28.09 ± 0.51%) and a decrease in random coil content (6.69 ± 0.92%) compared to α-helices (23.61 ± 0.83) and random coil structures (9.47 ± 1.48) in HIGs (p < 0.05). Only the PC treatment resulted in a significant reduction in total plate count (TPC) (1.51–1.58 log CFU/g) compared to 2.33 ± 0.33 log CFU/g in the HIG treatment. These findings suggest that HPP should be applied prior to thermal treatments (cooking or setting) to achieve an improved gel quality in reduced-salt pangasius products. Full article

Swine wastewater (SW) has a high chemical oxygen demand (COD) content and is difficult to degrade; an effective strategy to address this issue is through biodegradation, which poses negligible secondary pollution risks and ensures cost-efficiency. The objectives of this study were to isolate an effective COD-degrading strain of SW, characterize (at the molecular level) its transformation of SW, and apply it to practical production. A strain of Corynebacterium xerosis H1 was isolated and had a 27.93% ± 0.68% (mean ± SD) degradation rate of COD in SW. This strain precipitated growth in liquids, which has the advantage of not needing to be immobilized, unlike other wastewater-degrading bacteria. Based on analysis by Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS), this bacterium removed nitrogen-containing compounds in SW, with proteins and lipids decreasing from 41 to 10% and lignins increasing from 51 to 82%. Furthermore, the enhancement of the sequencing batch reactor (SBR) with strain H1 improved COD removal in effluent, with reductions in the fluorescence intensity of aromatic protein I, aromatic protein II, humic-like acids, and fulvic acid regions. In addition, based on 16S rRNA gene sequencing analysis, SBR_H1 successfully colonized some H1 bacteria and had a higher abundance of functional microbiota than SBR_C. This study confirms that Corynebacterium xerosis H1, as a carrier-free efficient strain, can be directly applied to swine wastewater treatment, reducing carrier costs and the risk of secondary pollution. The discovery of this strain enriches the microbial resource pool for SW COD degradation and provides a new scheme with both economic and environmental friendliness for large-scale treatment. Full article

Transcription factors (TFs) are proteins that control gene expression by binding to specific DNA sequences and are essential for cell development, differentiation, and homeostasis. Dysregulation of TFs is implicated in numerous diseases, including cancer, autoimmune disorders, and neurodegeneration. While TFs were traditionally considered “undruggable” due to their lack of well-defined binding pockets, recent advances have made it possible to modulate their activity using diverse pharmacological strategies. Major TF families include NF-κB, p53, STATs, HIF-1α, AP-1, Nrf2, and nuclear hormone receptors, which take part in the regulation of inflammation, tumor suppression, cytokine signaling, hypoxia and stress response, oxidative stress, and hormonal response, respectively. TFs can perform multiple functions, participating in the regulation of opposing processes depending on the context. NF-κB, for instance, plays dual roles in immunity and cancer, and is targeted by proteasome and IKKβ inhibitors. p53, often mutated in cancer, is reactivated using MDM2 antagonist Nutlin-3, refunctionalizing compound APR-246, or stapled peptides. HIF-1α, which regulates hypoxic responses and angiogenesis, is inhibited by agents like acriflavine or stabilized in anemia therapies by HIF-PHD inhibitor roxadustat. STATs, especially STAT3 and STAT5, are oncogenic and targeted via JAK inhibitors or novel PROTAC degraders, for instance SD-36. AP-1, implicated in cancer and arthritis, can be inhibited by T-5224 or kinase inhibitors JNK and p38 MAPK. Nrf2, a key antioxidant regulator, can be activated by agents like DMF or inhibited in chemoresistant tumors. Pharmacological strategies include direct inhibitors, activators, PROTACs, molecular glues, and epigenetic modulators. Challenges remain, including the structural inaccessibility of TFs, functional redundancy, off-target effects, and delivery barriers. Despite these challenges, transcription factor modulation is emerging as a viable and promising therapeutic approach, with ongoing research focusing on specificity, safety, and efficient delivery methods to realize its full clinical potential. Full article

Heavy metals are a major cause for concern in relation to water systems, due to their high toxicity at elevated levels. The metals can originate from both natural processes, including geological weathering and volcanic activity, as well as anthropogenic activi-ties such as industrial discharges, agricultural runoff, mining, and urbanization, which significantly contribute to water pollution and environmental degradation. The as-sessment of these risks is crucial for protecting public health, especially in populations reliant on contaminated water sources. Exposure to such contaminants can result in severe health consequences, including neurological impairments, organ deterioration, and an elevated risk of cancer. To conduct this assessment study, six surface water sampling sites were selected (i.e., S1 (Gobia), S2 (Kouamefla), S3 (Benkro), S4 (Dou-kouya), S5 (Doka), and S6 (Zengue)) due to their proximity to mining activities. We used the hazard quotient (HQ) and hazard index (HI) methods to estimate the levels of non-carcinogenic health risk associated with heavy metals. Then, the assessment of carcinogenic health risk was carried out using the Incremental Lifetime Cancer Risk (ILCR) methods. First, the highest ILCR total values were observed in the Doya locality (i.e., 0.4237 for the children and 0.5650 for the adults) and during the great dry season (i.e., 0.4333 for the children and 0.5743 for the adults). These findings highlight that populations in this locale experience heightened exposure during the period of the Great Rainy Season. The results indicated that the population exposed to Cd and Hg may experience health concerns irrespective of season and locality. For As and Pb, risks are present in both seasons (i.e., Short Dry Season (SDS) and Short Rainy Season (SRS)). On the other hand, the HIs are well above 1, indicating that the population may be exposed to non-carcinogenic diseases associated with the metals, regardless of the season or locality. To further explore the results, the assessment by ILCR was em-ployed, which demonstrated that for all the designated localities, the ILCRs of As and Cd are well above 10⁻⁴ for the entire population, indicating that the population con-suming this water may develop major carcinogenic risks. In addition, the highest ILCR values were obtained for Cd, regardless of the age group. It should be noted that sea-sonal variation had no significant effect on the trend in ILCRs determined for the en-tire population. Full article

To address the issue of aflatoxin contamination, which poses a significant threat to food safety and human health, we have conducted extensive research. We have isolated a strain of Myroides odoratimimus (3J2MO) from the soil that exhibited remarkable efficiency in degrading various aflatoxin types, including AFB1, AFB2, AFG1, AFG2, and AFM1. SDS-PAGE analysis confirmed the purity of the enzymes to be over 95%. Through fluorescence assays, we quantified the enzymatic activity, with an AFB1 degradation rate of 95% achieved at 37 °C and a pH of 8.0. Further analysis using HPLC-MS/MS identified the degradation intermediates, revealing the mechanisms of lactone ring cleavage and epoxy group hydrolysis. GO/COG/KEGG annotations provided insights into the functions of these enzymes, with peroxidase linked to reactive oxygen species (ROS) generation and helicase associated with ATP-dependent conformational changes. Helicase, on the other hand, hydrolyzes ATP, driving conformational changes in AFB1 and facilitating its breakdown into non-toxic metabolites. The potential industrial-scale application of this discovery could significantly mitigate aflatoxin-related economic losses while minimizing chemical residues in the food chain. Full article

Numerous remediation strategies exist for cyanobacterial harmful algal blooms (cyanoHABs); however, most are limited by challenges of scalability and adverse off-target effects on the surrounding ecosystem. Germicidal ultraviolet light (UV-C) has emerged as a promising method for suppressing cyanoHABs in a sustainable, chemical-free manner that is both scalable and results in limited off-target ecological effects in the surrounding area. In this study, the US Army Engineer Research and Development Center’s (ERDC)’s CyanoSTUN^TM (Cyanobacterial Suppression Through Ultraviolet-Light-C Neutralization) vessel was deployed to a cyanoHAB as part of a field trial to determine whether UV-C could effectively suppress cellular growth, degrade associated cyanotoxins, and inhibit harmful phytoplankton species more readily than beneficial species without the addition of chemicals. The cyanoHAB exhibited an average cyanobacteria abundance of 3.75 × 10⁵ cells/mL (n = 5, SD = 6.76 × 10⁴ cells/mL) and average total microcystin concentration of 3.5 µg/L (n = 5; SD = 0.24 µg/L). Pre- and post-treatment samples were collected and re-grown for 9 days in the laboratory to observe differences in microcystin, chlorophyll a, and phycocyanin concentrations, optical density, cell density, and community composition. The results of the field trial showed that the CyanoSTUN UV-C treatment effectively suppressed the growth of the cyanobacteria community for approximately two days at the three tested UV-C doses. The CyanoSTUN UV-C treatment also demonstrated a sustained, dose-dependent effect on microcystin concentration; the average reduction in microcystin concentration for 15, 30, and 45 mJ/cm² treatment doses was 31.6% (n = 10, SD = 20.1%; 1.3 µg/L reduced), 45.7% (n = 10, SD = 10.8%; 1.9 µg/L reduced), and 49.9% (n = 10, SD = 8.2%; 1.7 µg/L reduced), respectively, over the 9-day regrowth period. Non-cyanobacteria were too scarce in this CyanoHAB to conclude whether the CyanoSTUN UV-C inhibits harmful phytoplankton species more readily than beneficial species. Further field studies with the CyanoSTUN^TM are required to validate performance under more severe cyanoHAB conditions, however the results reported herein from the first field trial with the CyanoSTUN^TM suggest that this treatment method may offer water managers confronted with a CyanoHAB the ability to rapidly and safely pause a bloom for multiple days and reduce the risks posed by its associated cyanotoxins without adding chemicals. Full article

Yak meat jerky, a traditional high-protein food commonly consumed in high-altitude regions, is often produced via air-drying, which may adversely affect its nutritional quality and digestibility. This study systematically compared the gastrointestinal digestion profiles of air-dried yak meat (ADM) and vacuum freeze-dried yak meat (VFDM) using a dynamic in vitro human stomach–intestine (DHSI-IV) system. Key digestive parameters, including gastric emptying kinetics, particle size distribution, and protein hydrolysis, were evaluated under physiologically relevant conditions. VFDM exhibited superior hydration capacity, contributing to delayed gastric emptying of the mixed solid–liquid phase (t_1/2 = 85.1 ± 1.0 min) compared to ADM (t_1/2 = 80.4 ± 1.2 min), indicating increased gastric satiety. Conversely, VFDM showed a faster solid-phase gastric emptying (t_1/2 = 107.2 ± 0.8 min) relative to ADM (t_1/2 = 113.1 ± 2.7 min), likely due to improved texture and rehydration. Both jerky types exhibited progressive particle disintegration; by 180 min, large particles (>2.0 mm) decreased to 16.88% ± 2.63% in ADM and 20.04% ± 0.64% in VFDM (p > 0.05). Protein digestibility, measured by SDS-PAGE and the degree of hydrolysis (DH), was significantly higher in VFDM (38.5 ± 3.6%) than in ADM (34.0 ± 0.1%, p < 0.05), with VFDM demonstrating more rapid and extensive protein degradation across gastric and intestinal phases. These improvements may be attributed to the porous microstructure and reduced processing-induced protein cross-linking in VFDM, facilitating enhanced enzyme access. Overall, vacuum freeze-drying substantially improved yak jerky protein digestibility, offering the potential for the development of meat-based functional foods targeted at individuals with compromised gastrointestinal function. Full article

Laccase catalyzes one-electron oxidation, producing water as the primary final product, thereby minimizing secondary environmental pollution. Consequently, it holds significant application potential in areas such as the degradation of toxic compounds. In this study, a high-laccase-producing Trichoderma strain was isolated from soil, and the conditions for laccase production were optimized. Additionally, the laccase-related gene was cloned, and its function was analyzed. The results revealed that the optimal conditions for laccase production in this strain were maltose as the carbon source, peptone as the nitrogen source, an optimal pH of 6.0, and an incubation time of 120 h, resulting in an enzyme activity of 1.32 U/mL. The purified enzyme exhibited a Michaelis constant (K_m) of 0.06666 mmol/L when ABTS was used as the substrate. SDS-PAGE analysis indicated that the enzyme’s molecular weight was approximately 70 kDa. Sequencing of the target protein band led to the identification of the laccase-related gene Tasla01. Knockout of this gene resulted in the loss of laccase activity. We isolated a high-laccase-producing Trichoderma asperellum strain, Tasjk65, and cloned the laccase-related functional gene Tasla01. These findings lay a foundation for the source and application of laccase. Full article

Backgroud: Glutamate dehydrogenase (GDH) is involved in the metabolism of glutamate and ammonia. It is regulated by multiple ligand variants, and hyper-active GDH mutants have been reported for hyperinsulinism hyperammonemia syndrome (HHS). Methods: Here, we constructed the wild-type human GDH and three human GDH454 mutants and investigated their degradation activity and performance under different GDH inhibitors. Results: Protein activity test and SDS-PAGE analysis of the purified proteins showed that the GDH454 mutant from HHS has weaker GDH enzymatic activity but greater resistance to trypsin hydrolysis than the wild type. Interestingly, using the biomolecular interactions technique, it showed that the GDH454 mutant has 10⁹ times weaker affinity for trypsin and 10-fold weaker for epigallocatechin gallate (EGCG) than the wild-type GDH. Subsequently, native-PAGE gel analysis demonstrated that EGCG could break down the GDH hexamer into monomers and form a complex with trypsin to enhance the degradation of both types of GDH. Conclusions: EGCG showed good affinity to both the wild-type and the mutant GDH proteins, promoting protein degradation; this provides a new strategy for the treatment of HHS and other hyper-active GDH-related diseases. Full article

Soil washing is an efficient method to remove polybrominated diphenyl ethers (PBDEs) from contaminated soils. The obtained solutions from soil-washing still contain PBDEs, requiring further treatment before disposal or reuse. Although photolysis is effective for PBDE degradation in solutions, the concurrent formation of toxic polybrominated dibenzofurans (PBDFs) may limit its practical application. In this study, 2,8-dibromodibenzofurans (2,8-BDF) formation rate and mechanisms during 2,4,4′-tribromodiphenyl ether (BDE-28) photolysis in various simulated soil-washing solutions was investigated. Results revealed significant effects of solubilizers on 2,8-BDF formation. The nonionic surfactants polysorbate (TW80), polyoxyethylene octylphenyl ether (TX series), and the cationic surfactant cetyltrimethylammonium bromide (CTAB) resulted in low 2,8-BDF formation rate (1–5%), while the β-cyclodextrin led to the highest 2,8-BDF formation rate (about 28%). The nonionic surfactants polyoxyethylene dodecyl ethers (Brij series), and the anionic surfactants sodium dodecylbenzene sulfonate (SDBS) and sodium dodecyl sulfate (SDS), also showed a high level of 2,8-BDF formation rate (7–17%). Solubilizer structure and its interaction with BDE-28 determined the 2,8-BDF formation. The role of the micelle microenvironment on 2,8-BDF formation was verified via an experiment and molecular dynamics simulation. The organic region of micelle exhibited high hydrogen donation ability, which inhibited 2,8-BDF formation. The results indicated distinct risks of PBDE photolysis in various soil-washing solutions, providing an important reference for solubilizer selection and the application of photolysis on the treatment of soil-washing solutions containing PBDEs. Full article

While the methods for monitoring deforestation are relatively well established, there is still no compromise on those for forest degradation. We propose here a systematic review on studies about forest degradation in the Congo Basin. Our analysis focused on seven key anthropogenic causes of forest degradation. Shifting agriculture emerged as the most significant driver, accounting for 61% ± 28.58% (mean ± SD) of canopy opening, 73.16% ± 16.88% aboveground carbon loss, and 30.37% ± 30.67% of tree species diversity loss over a 5–60-year period. Our analysis reveals a significant disconnect. Only 29% of the reviewed studies address this driver, while over 64% focus primarily on the consequences of industrial timber harvesting. Despite its comparatively minor contribution to degradation, with effects range from only 8.98% ± 13.63% of canopy opening, 14.79% ± 22.21 aboveground carbon loss, and 4.27 ± 21.07 tree species diversity loss over 1–20 years. Indeed, most of the methods focus on detecting changes in canopy structure associated with forest logging over a short period (0–5 years). These illustrate the need for a shift in focus in scientific research towards innovative methods, which can be developed over time, to monitor the various impacts of all causes of forest degradation. Full article

This study aimed to characterize and evaluate the fibrinolytic, thrombolytic, hematological, and toxicological aspects of a serine protease (AsKSP) from Aspergillus tamarii Kita UCP 1279. The enzyme was purified using a two-phase aqueous system and assessed for optimal pH (7.0) and temperature (50 °C), stability, and effects of metal ions, inhibitors, and surfactants. AsKSP exhibited stability for up to 120 min at 50 °C and 36 h at pH 7.0. Enzymatic activity was enhanced by Na⁺ and Zn²⁺ and non-ionic surfactants (Tween-80) but inhibited by Cu²⁺, Fe³⁺, Triton X-100, and SDS, reducing activity by up to 62.35%. The highest amidolytic activity was observed for the substrate N-succinyl-Gly–Gly–Phe-p-nitroanilide. SDS-PAGE analysis indicated an approximate molecular mass of 90 kDa. The enzyme showed fibrinolytic activity, degrading 38.81% of fibrin clots in vitro after 90 min, without affecting fibrinogen. Cytotoxicity assays indicated no toxicity (cell viability > 80%). Coagulation assays showed slight prolongation of prothrombin time (PT) and activated partial thromboplastin time (aPTT), with no effect on thrombin time. No red blood cell lysis was observed, and albumin increased enzymatic activity by 31.70%. These findings demonstrate that Aspergillus tamarii Kita UCP 1279 produces a fibrinolytic protease with potential for thrombus treatment, providing a promising foundation for drug development. Full article

Accurate identification of mattic epipedon degradation is critically important for addressing ecological issues such as the weakening of alpine grassland carbon sink capacity and reduced soil and water conservation. However, efficient and rapid methods for its detection remain limited. This study aimed to clarify the hyperspectral response mechanisms of mattic epipedon degradation and, based on hyperspectral technology, to construct models for identifying degraded mattic epipedon and screen preprocessing methods suitable for different moisture conditions. The results showed the following: (1) The XGBoost model with preprocessing using multiplicative scatter correction combined with second derivative transformation (MSC+SD) performed best, achieving an identification accuracy and Kappa coefficient of 0.85 and 0.82, respectively. The characteristic bands were concentrated in the visible light range (446–450 nm) and short-wave infrared range (2134 nm, 2267–2269 nm), which are closely related to the spectral responses of organic carbon and mineral components. (2) Spectral reflectance was significantly negatively correlated with moisture content, and model accuracy decreased as moisture content increased. (3) After correction using the EPO algorithm, the model accuracy for the high-moisture group improved by 13.2–16.7%, whereas that for the low-moisture group (<15%) decreased by 7.5%, verifying 15% moisture content as the critical threshold for water interference. This study elucidated the impact mechanism of moisture on the hyperspectral characteristics of the mattic epipedon. The established MSC+SD-XGBoost model adapts to varying moisture conditions, providing technical support for the rapid monitoring of mattic epipedon degradation and holding significant practical value for carbon management in alpine ecosystems. Full article

The periodate/hydrogen peroxide (PI/H₂O₂) system is a recently developed advanced oxidation process (AOP) characterized by its rapid reaction kinetics, making it highly suitable for continuous-flow applications compared to conventional batch systems. Despite its potential, no prior studies have investigated its performance under flowing conditions. This work presents the first application of the PI/H₂O₂ process in a tubular microreactor, a promising technology for enhancing mass transfer and process efficiency. The degradation of textile dyes (specifically Basic Yellow 28 (BY28)) was systematically evaluated under various operating conditions, including reactant concentrations, flow rates, reactor length, and temperature. The results demonstrated that higher H₂O₂ flow rates, increased PI dosages, and moderate dye concentrations (25 µM) significantly improved degradation efficiency, achieving complete mineralization at 2 mM PI and H₂O₂ flow rates of 80–120 µL/s. Conversely, elevated temperatures negatively impacted the process performance. The influence of organic and inorganic constituents was also examined, revealing that surfactants (SDS, Triton X-100, Tween 20, and Tween 80) and organic compounds (sucrose and glucose) acted as strong hydroxyl radical scavengers, substantially inhibiting dye oxidation—particularly at higher concentrations, where nearly complete suppression was observed. Furthermore, the impact of water quality was assessed using different real matrices, including tap water, seawater, river water, and secondary effluents from a municipal wastewater treatment plant (SEWWTP). While tap water exhibited minimal inhibition, river water and SEWWTP significantly reduced process efficiency due to their high organic content competing with reactive oxygen species (ROS). Despite its high salt content, seawater remained a viable medium for dye degradation, suggesting that further optimization could enhance process performance in saline environments. Overall, this study highlights the feasibility of the PI/H₂O₂ process in continuous-flow microreactors and underscores the importance of considering competing organic and inorganic constituents in real wastewater applications. The findings provide valuable insights for optimizing AOPs in industrial and municipal wastewater treatment systems. Full article