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10 pages, 1375 KB  
Proceeding Paper
Mapping Soil Moisture Using Drones: Challenges and Opportunities
by Ricardo Díaz-Delgado, Pauline Buysse, Thibaut Peres, Thomas Houet, Yannick Hamon, Mikaël Faucheux and Ophelie Fovert
Eng. Proc. 2025, 94(1), 18; https://doi.org/10.3390/engproc2025094018 (registering DOI) - 25 Aug 2025
Abstract
Droughts are becoming more frequent, severe, and impactful across the globe. Agroecosystems, which are human-made ecosystems with high water demand that provide essential ecosystem services, are vulnerable to extreme droughts. Although water use efficiency in agriculture has increased in rec ent decades, drought [...] Read more.
Droughts are becoming more frequent, severe, and impactful across the globe. Agroecosystems, which are human-made ecosystems with high water demand that provide essential ecosystem services, are vulnerable to extreme droughts. Although water use efficiency in agriculture has increased in rec ent decades, drought management should be based on long-term, proactive strategies rather than crisis management. The AgrHyS network of sites in French Brittany collects high-resolution soil moisture data from agronomic stations and catchments to improve understanding of temporal soil moisture dynamics and enhance water use efficiency. Frequent mapping of soil moisture and plant water stress is crucial for assessing water stress risk in the context of global warming. Although satellite remote sensing provides reliable, periodic global data on surface soil moisture, it does so at a very coarse spatial resolution. The intrinsic spatial heterogeneity of surface soil moisture requires a higher spatial resolution in order to address upcoming challenges on a local scale. Drones are an excellent tool for upscaling point measurements to catchment level using different onboard cameras. In this study, we evaluated the potential of multispectral images, thermal images and LiDAR data captured in several concurrent drone flights for high-resolution mapping of soil moisture spatial variability, using in situ point measurements of soil water content and plant water stress in both agricultural areas and natural ecosystems. Statistical models were fitted to map soil water content in two areas: a natural marshland and a grassland-covered agricultural field. Our results demonstrate the statistical significance of topography, land surface temperature and red band reflectance in the natural area for retrieving soil water content. In contrast, the grasslands were best predicted by the transformed normalised difference vegetation index (TNDVI). Full article
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14 pages, 1507 KB  
Article
Grafting ‘Red Globe’ (Vitis vinifera) onto Multiple Rootstocks: A Systematic, Multi-Year Evaluation Focusing on Graft Compatibility, Vegetative Growth, and Fruit Characteristics
by Yonggang Yin, Junwei Yuan, Nan Jia, Minmin Li, Changjiang Liu, Yan Sun, Xinyu Wang, Shuli Han, Qian Gao, Shiyuan Liu and Bin Han
Horticulturae 2025, 11(9), 1006; https://doi.org/10.3390/horticulturae11091006 - 25 Aug 2025
Abstract
Selecting appropriate rootstocks can enhance the adaptability and fruit quality of grafted grapevines. However, grafting studies on ‘Red Globe’, one of the major cultivated cultivars, remain limited, particularly those involving long-term and comprehensive evaluations. The present research grafted ‘Red Globe’ onto four rootstocks—‘101-14’, [...] Read more.
Selecting appropriate rootstocks can enhance the adaptability and fruit quality of grafted grapevines. However, grafting studies on ‘Red Globe’, one of the major cultivated cultivars, remain limited, particularly those involving long-term and comprehensive evaluations. The present research grafted ‘Red Globe’ onto four rootstocks—‘101-14’, ‘188-08’, ‘110R’, and ‘3309C’—and systematically compared graft union healing following hardwood grafting, field performance of grafted vines, vegetative growth of mature vines, and fruit phenotypic and quality traits across multiple years. The results showed that ‘101-14’ promoted the accumulation of organic acids, which reached 1.1% in 2023, and caused an increased tendency for berry detachment from the peduncle. The RG/110R combination exhibited a higher CFI, 0.8 on average, at the basal section, and promoted shoot thickening. RG/3309C was found to have a larger shoot length exceeding 600 cm, and a significant increase in fruit weight to nearly 13 g. The grafts on ‘188-08’ showed the highest survival rate of 74% among the graft combinations, and enhanced fruit quality, as evidenced by elevated TSS (16 °Brix) and firmer pulp texture, indicating that ‘188-08’ may serve as a valuable rootstock for enhancing the local adaptability and fruit quality of ‘Red Globe’ grapevines. Full article
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14 pages, 4788 KB  
Article
Heat Impact Assessment and Heat Prevention Suggestions for Thermal Comfort at Large-Area and Long-Duration Outdoor Sport Events in Taiwan
by Si-Yu Yu, Tzu-Ping Lin and Andreas Matzarakis
Atmosphere 2025, 16(7), 805; https://doi.org/10.3390/atmos16070805 - 1 Jul 2025
Viewed by 475
Abstract
This study aims to (1) analyze thermal comfort at outdoor sport events held outside of fixed venues or locations; (2) establish a method for evaluating environmental thermal comfort for large-scale, long-term outdoor activities; and (3) provide suggestions for the arrangement of shifts in [...] Read more.
This study aims to (1) analyze thermal comfort at outdoor sport events held outside of fixed venues or locations; (2) establish a method for evaluating environmental thermal comfort for large-scale, long-term outdoor activities; and (3) provide suggestions for the arrangement of shifts in routes and participants for heat warning and mitigation. Taiwan ReAnalysis Downscaling (TReAD) data, Sky View Factors (SVFs), GSV2SVF tool, and RayMan Pro were applied to analyze and evaluate thermal comfort at the 2021 Torch Relay Round the Island, Taiwan. In this study, modified Physiologically Equivalent Temperature (mPET), Wet Bulb Globe Temperature (WBGT), and Universal Thermal Climate Index (UTCI) were estimated and selected as thermal indicators for the purpose of obtaining a more comprehensive perspective. We also define and present thermal performance with a simple traffic light symbol (green: comfortable/yellow: warm/red: hot) and try to go beyond the concept of heat and visualize it in an easy-to-understand way. Full article
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20 pages, 1033 KB  
Review
Crosstalk Between Sickle Cell Disease and Ferroptosis
by Annamaria Russo, Giuseppe Tancredi Patanè, Antonella Calderaro, Davide Barreca, Ester Tellone and Stefano Putaggio
Int. J. Mol. Sci. 2025, 26(8), 3675; https://doi.org/10.3390/ijms26083675 - 13 Apr 2025
Cited by 1 | Viewed by 1100
Abstract
Sickle cell disease (SCD) is an inherited hemoglobin disorder that is widespread across the globe. It is characterized by a very complex pathogenesis, but at the basis of the disease is the mutation of the HBB gene, which determines the production of a [...] Read more.
Sickle cell disease (SCD) is an inherited hemoglobin disorder that is widespread across the globe. It is characterized by a very complex pathogenesis, but at the basis of the disease is the mutation of the HBB gene, which determines the production of a mutated hemoglobin: sickle cell hemoglobin (HbS). The polymerization of HbS, which occurs when the protein is in a deoxygenated state, and the greater fragility of sickle cell red blood cells (sRBCs) determine the release of iron, free heme, and HbS in the blood, favoring oxidative stress and the production of reactive oxygen species (ROS). These features are common to the features of a new model of cell death known as ferroptosis, which is characterized by the increase of iron and ROS concentrations and by the inhibition of glutathione peroxidase 4 (GPx4) and the System Xc. In this context, this review aims to discuss the potential molecular and biochemical pathways of ferroptosis involved in SCD, aiming to highlight possible tags involved in treating the disease and inhibiting ferroptosis. Full article
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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21 pages, 1127 KB  
Article
Efficient Compression of Red Blood Cell Image Dataset Using Joint Deep Learning-Based Pattern Classification and Data Compression
by Zerin Nusrat, Md Firoz Mahmud and W. David Pan
Electronics 2025, 14(8), 1556; https://doi.org/10.3390/electronics14081556 - 11 Apr 2025
Viewed by 563
Abstract
Millions of people across the globe are affected by the life-threatening disease of Malaria. To achieve the remote screening and diagnosis of the disease, the rapid transmission of large-size microscopic images is necessary, thereby demanding efficient data compression techniques. In this paper, we [...] Read more.
Millions of people across the globe are affected by the life-threatening disease of Malaria. To achieve the remote screening and diagnosis of the disease, the rapid transmission of large-size microscopic images is necessary, thereby demanding efficient data compression techniques. In this paper, we argued that well-classified images might lead to higher overall compression of the images in the datasets. To this end, we investigated the novel approach of joint pattern classification and compression of microscopic red blood cell images. Specifically, we used deep learning models, including a vision transformer and convolutional autoencoders, to classify red blood cell images into normal and Malaria-infected patterns, prior to applying compression on the images classified into different patterns separately. We evaluated the impacts of varying classification accuracy on overall image compression efficiency. The results highlight the importance of the accurate classification of images in improving overall compression performance. We demonstrated that the proposed deep learning-based joint classification/compression method offered superior performance compared with traditional lossy compression approaches such as JPEG and JPEG 2000. Our study provides useful insights into how deep learning-based pattern classification could benefit data compression, which would be advantageous in telemedicine, where large-image-size reduction and high decoded image quality are desired. Full article
(This article belongs to the Special Issue Deep Learning-Based Image Restoration and Object Identification)
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21 pages, 1121 KB  
Article
A Systematic Mapping-Driven Framework for Vetting Participation in Business Ecosystems
by Margaret Mastropetrou, Konstadinos Kutsikos and George Bithas
Systems 2025, 13(4), 236; https://doi.org/10.3390/systems13040236 - 29 Mar 2025
Viewed by 525
Abstract
A key strategic option for many organizations across the globe is to examine whether and how business ecosystems can help them survive and thrive amidst continuous changes in business realities. Joining a business ecosystem, though, is not a straightforward decision. Current research efforts [...] Read more.
A key strategic option for many organizations across the globe is to examine whether and how business ecosystems can help them survive and thrive amidst continuous changes in business realities. Joining a business ecosystem, though, is not a straightforward decision. Current research efforts are falling short of fully identifying a concise and practical set of decision-making factors that potential ecosystem participants can meaningfully use. To address this limitation, the authors developed a framework of decision-making factors (motivations, prerequisites, ecosystem attractiveness), based on (a) their findings of a systematic mapping study they conducted and (b) their parallel research efforts in business ecosystems operations. The proposed framework encompasses a concrete “vocabulary” of decision-making factors that can enable complex “dialogs” between existing and new business ecosystem stakeholders. As a result, this research effort (a) offers a clear and unambiguous categorization of previously overloaded and ambiguous decision-making factors; (b) captures relationships between the three core components of the proposed framework, thus considering upfront any synergies or conflicts among them; and (c) makes the candidate organization’s decision-making process pragmatic, i.e., misalignment among the proposed factors should be considered a ‘red flag’ that may drive the candidate organization to pivot its decision-making process towards another business ecosystem. Full article
(This article belongs to the Special Issue Business Model Innovation in the Digital Era)
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18 pages, 678 KB  
Review
Red Meat Amino Acids for Beginners: A Narrative Review
by Benjamin Barr, Danielle E. Levitt and Lauren Gollahon
Nutrients 2025, 17(6), 939; https://doi.org/10.3390/nu17060939 - 7 Mar 2025
Cited by 2 | Viewed by 3686
Abstract
Meat is a major source of dietary protein and fat across the globe. Red and white meat are the major terms consumers use to refer to types of meat; however, these terms do not fully encompass the range of nutrients provided by meat [...] Read more.
Meat is a major source of dietary protein and fat across the globe. Red and white meat are the major terms consumers use to refer to types of meat; however, these terms do not fully encompass the range of nutrients provided by meat sources. Red meat refers to meat from mammalian skeletal muscle, while white meat refers to poultry. Red and white meat both provide a wide range of nutritional components in the context of fatty acids, amino acids and micronutrients. Importantly, it has been demonstrated that amino acid profiles differ between red meat and white meat as well as between different sources of red meat. Red meat is a complete source of dietary amino acids, meaning it contains all essential amino acids (EAAs), and in addition, it contains all the non-essential amino acids (NEAAs). Red meat is also the most abundant source of bioavailable heme-iron essential for muscle growth and cardiovascular health. Red meat has been indicated as a major contributor to the rising incidence of metabolic disorders and even colorectal cancer. However, it is important to note that while red meat consumption is linked to these conditions, it is typically the overconsumption of red meat that is associated with obesity and other metabolic symptoms. Similarly, the preparation of red meat is a key factor in its link to colorectal cancer as some methods of preparation produce carcinogens while others do not. Finally, red meat may also be situationally more beneficial to some groups than others, particularly in the cases of sex and aging. For pregnant women, increases in red meat consumption may be beneficial to increase the intake of semi-essential amino acids, while in the elderly, increases in red meat consumption may better preserve muscle mass compared with other dietary protein sources. Full article
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19 pages, 7714 KB  
Article
Production of Soft Magnetic Materials Fe-Si and Fe-Si-Al from Blends of Red Muds and Several Additives: Resources for Advanced Electrical Devices
by Rita Khanna, Yuri Konyukhov, Dmitri Zinoveev, Kejiang Li, Nikita Maslennikov, Igor Burmistrov, Jumat Kargin, Maksim Kravchenko and Partha Sarathy Mukherjee
Sustainability 2025, 17(5), 1795; https://doi.org/10.3390/su17051795 - 20 Feb 2025
Cited by 1 | Viewed by 914
Abstract
The present study developed a novel approach for transforming red mud (RM) into soft magnetic materials (SMMs) for applications in advanced electrical devices in the form of Fe-Si and Fe-Si-Al alloys. A total of ten blends were prepared based on two RMs, three [...] Read more.
The present study developed a novel approach for transforming red mud (RM) into soft magnetic materials (SMMs) for applications in advanced electrical devices in the form of Fe-Si and Fe-Si-Al alloys. A total of ten blends were prepared based on two RMs, three iron oxide additives (Fe2O3, black and red mill scales), alumina and carbonaceous reductants in a range of proportions. Carbothermic reduction of the blends was carried out in a vertical Tamman resistance furnace at 1600–1650 °C for 30 min in an argon atmosphere; synthetic graphite was used as a reductant. Reaction products were characterized using scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray fluorescence (XRF) and X-ray diffraction (XRD). Significant amounts of Fe-rich metallic droplets/regions of different grain sizes (0.5 to 500 μm) were produced in these studies. The formation of Fe-Si alloys with Si contents from 3.9 to 6.7 wt.% was achieved in 8 out of 10 blends; the optimal levels of Si for SMMs ranged from 3.2 to 6.5 wt.%. There was clear evidence for the formation of Fe-Si-Al (up to 1.8 wt.% Al) alloys in 4 out of 10 blends. In addition to lowering operating challenges associated with RM processing, blending of RMs with iron oxide additives and alumina presents a novel recycling approach for converting RMs into valuable SMMs for possible emerging applications in renewable energy, storage, electrical vehicles and other fields. Along with reducing RM stockpiles across the globe, this approach is expected to improve resource efficiency, mitigating environmental impacts while generating economic benefits. Full article
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18 pages, 3058 KB  
Article
Impact of Deactivated Mine Waste Substrates on the Growth and Cu, As and Pb Accumulation in Tubers, Roots, Stems and Leaves of Three Solanum tuberosum L. Varieties
by Ana R. F. Coelho, Manuela Simões, Fernando H. Reboredo, José Almeida, Joaquim Cawina and Fernando Lidon
Plants 2025, 14(2), 230; https://doi.org/10.3390/plants14020230 - 15 Jan 2025
Cited by 1 | Viewed by 870
Abstract
Potato (Solanum tuberosum L.) is the world’s third most popular vegetable in terms of consumption and the fourth most produced. Potatoes can be easily cultivated in different climates and locations around the globe and often in soils contaminated by heavy metals due [...] Read more.
Potato (Solanum tuberosum L.) is the world’s third most popular vegetable in terms of consumption and the fourth most produced. Potatoes can be easily cultivated in different climates and locations around the globe and often in soils contaminated by heavy metals due to industrial activities. This study assessed heavy metal accumulation in different organs of three S. tuberosum L. varieties (Agria, Désirée, and Red Lady) grown in different substrate formulations containing slag and waste from the Caveira polymetallic sulfite mine in Portugal. Results reveal that Cu, Pb, and As accumulation in the different organs of the plant depends on variety and substrate formulation, with tubers exceeding reference values from the literature. Tubers accumulated less Cu (varying between 17.3 and 32 mg/kg), Pb (varying between 5 and 27.6 mg/kg) and As (varying between 4 and 14.8 mg/kg) compared to other plant organs, and the Désirée variety exhibited high Pb (with a maximum of 27.6 mg/kg) accumulation in tubers compared to the remaining varieties. Although the phenological development of plants was not impacted, substrate formulation played a critical role in the plant’s metal uptake. The Agria variety presented a lower contamination risk in tubers, but potato cultivation in contaminated soils can present a risk to human health. Full article
(This article belongs to the Section Plant Physiology and Metabolism)
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18 pages, 4315 KB  
Article
Real-Time Monitoring of Environmental Parameters in Schools to Improve Indoor Resilience Under Extreme Events
by Salit Azoulay Kochavi, Oz Kira and Erez Gal
Smart Cities 2025, 8(1), 7; https://doi.org/10.3390/smartcities8010007 - 3 Jan 2025
Cited by 2 | Viewed by 2194
Abstract
Climatic changes lead to many extreme weather events throughout the globe. These extreme weather events influence our behavior, exposing us to different environmental conditions, such as poor indoor quality. Poor indoor air quality (IAQ) poses a significant concern in the modern era, as [...] Read more.
Climatic changes lead to many extreme weather events throughout the globe. These extreme weather events influence our behavior, exposing us to different environmental conditions, such as poor indoor quality. Poor indoor air quality (IAQ) poses a significant concern in the modern era, as people spend up to 90% of their time indoors. Ventilation influences key IAQ elements such as temperature, relative humidity, and particulate matter (PM). Children, considered a vulnerable group, spend approximately 30% of their time in educational settings, often housed in old structures with poorly maintained ventilation systems. Extreme weather events lead young students to stay indoors, usually behind closed doors and windows, which may lead to exposure to elevated levels of air pollutants. In our research, we aim to demonstrate how real-time monitoring of air pollutants and other environmental parameters under extreme weather is important for regulating the indoor environment. A study was conducted in a school building with limited ventilation located in an arid region near the Red Sea, which frequently suffers from high PM concentrations. In this study, we tracked the indoor environmental conditions and air quality during the entire month of May 2022, including an extreme outdoor weather event of sandstorms. During this month, we continuously monitored four classrooms in an elementary school built in 1967 in Eilat. Our findings indicate that PM2.5 was higher indoors (statistically significant) by more than 16% during the extreme event. Temperature was also elevated indoors (statistically significant) by more than 5%. The parameters’ deviation highlights the need for better indoor weather control and ventilation systems, as well as ongoing monitoring in schools to maintain healthy indoor air quality. This also warrants us as we are approaching an era of climatic instability, including higher occurrence of similar extreme events, which urge us to develop real-time responses in urban areas. Full article
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22 pages, 4788 KB  
Article
City Residents Play a Pivotal Role in Managing Global Food Security While Improving Human Health and Minimizing Environmental Footprints
by Jan-Olof Drangert
Nutrients 2024, 16(23), 4176; https://doi.org/10.3390/nu16234176 - 30 Nov 2024
Viewed by 1755
Abstract
Background/Objectives: Improved global data allow for a new understanding of what impact the food we produce, eat and dispose of has on the environment, human health and Nature’s resources. The overall goal is to guide decision-makers and individuals by providing in-depth knowledge about [...] Read more.
Background/Objectives: Improved global data allow for a new understanding of what impact the food we produce, eat and dispose of has on the environment, human health and Nature’s resources. The overall goal is to guide decision-makers and individuals by providing in-depth knowledge about the effects of their dietary preferences on human and environmental health. Methods: The method is to investigate ways to reduce environmental degradation and to secure healthy food supplies in an urbanizing world, and to quantify the options. Results: Reviewed articles show that by eating less meat-based food and more plant-based and soilless food, as well as reducing food waste and recycling urban-disposed nutrients as fertilizers, we could reduce agriculture’s land requirement by 50% to 70% while still securing a healthy food supply. Less land under cultivation and pasture would reduce global emissions to air and water to a similar extent, and allow Nature to reclaim freed areas in order to catch more carbon and rejuvenate biodiversity. Thus, we could avoid further environmental degradation such as the current clearing of new fields needed under a business-as-usual regime. Presently, some 17 million people die each year due to poor diets, which is more than double the 7 million deaths since the onset of the COVID-19 pandemic. A return to more plant-based diets with unchanged intake of proteins but less calories, sugar, salt and fat combined with less red meat and ultra-processed food would reduce foremost non-communicable diseases by up to 20% and prolong life. The article suggests that the international focus has gradually turned to the food sector’s big contribution to climate change, biodiversity loss and harmful chemicals as well as to poor human health. It argues that this century’s rapid population growth and urbanization give urban residents a pivotal role in food’s impact on agricultural areas, which today cover half of the globe’s inhabitable areas. Their food demand, rather than the activities of farmers, fishermen or loggers, will guide remedial measures to be taken by individuals, industry and the public sector. A tool to calculate the potential environmental footprints of individual or societal measures is presented. Conclusions: Measures to make the agrifood sector more sustainable are still pending full recognition in international fora such as the UN COP Summits. Smart cities fitted with infrastructures to recycle macro- and micro-nutrients and organic matter have the potential to ameliorate human-induced impacts such as emissions to air and water bodies, crossing planetary boundaries, and polluting extraction of N (nitrogen), P (phosphorus) and K (potassium). Rapid results are within reach since dietary change and the turn-around time of nutrients in food is short compared to decades or centuries for recycled materials in cars or buildings. Full article
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17 pages, 3440 KB  
Article
Time-Course Transcriptome Analysis Reveals Distinct Transcriptional Regulatory Networks in Resistant and Susceptible Grapevine Genotypes in Response to White Rot
by Tinggang Li, Xing Han, Lifang Yuan, Xiangtian Yin, Xilong Jiang, Yanfeng Wei and Qibao Liu
Int. J. Mol. Sci. 2024, 25(21), 11536; https://doi.org/10.3390/ijms252111536 - 27 Oct 2024
Viewed by 1210
Abstract
Grapevine (Vitis vinifera L.) is a globally significant economic crop. However, its widely cultivated varieties are highly susceptible to white rot disease. To elucidate the mechanisms of resistance in grapevine against this disease, we utilized time-ordered gene co-expression network (TO-GCN) analysis to [...] Read more.
Grapevine (Vitis vinifera L.) is a globally significant economic crop. However, its widely cultivated varieties are highly susceptible to white rot disease. To elucidate the mechanisms of resistance in grapevine against this disease, we utilized time-ordered gene co-expression network (TO-GCN) analysis to investigate the molecular responses in the grapevine varieties ‘Guifeimeigui’ (GF) and ‘Red Globe’ (RG). An assessment of their resistance demonstrated that GF is highly resistant to white rot, whereas RG is highly susceptible. We conducted transcriptome sequencing and a TO-GCN analysis on leaf samples from GF and RG at seven time points post-infection. Although a significant portion of the differentially expressed genes related to disease resistance were shared between GF and RG, the GF variety rapidly activated its defense mechanisms through the regulation of transcription factors during the early stages of infection. Notably, the gene VvLOX3, which is a key enzyme in the jasmonic acid biosynthetic pathway, was significantly upregulated in GF. Its upstream regulator, Vitvi08g01752, encoding a HD-ZIP family transcription factor, was identified through TO-GCN and yeast one-hybrid analyses. This study provides new molecular insights into the mechanisms of grapevine disease resistance and offers a foundation for breeding strategies aimed at enhancing resistance. Full article
(This article belongs to the Special Issue Power Up Plant Genetic Research with Genomic Data 2.0)
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11 pages, 1442 KB  
Article
Effects of Ethylene Inhibitors on the Long-Term Maintenance of the Embryogenic Callus of Vitis vinifera L.
by Lucia Rosaria Forleo, Teodora Basile, Bruna Suriano, Flavia Angela Maria Maggiolini, Margherita D’Amico, Maria Francesca Cardone, Riccardo Velasco and Carlo Bergamini
Horticulturae 2024, 10(10), 1038; https://doi.org/10.3390/horticulturae10101038 - 29 Sep 2024
Viewed by 1451
Abstract
Vitis vinifera is an important fruit crop which is mainly consumed fresh or used for the production of wine. Genetic improvement programs through New Genomic Techniques (NGTs) aim to develop grapevine varieties resistant to biotic and abiotic stresses or enhancing nutraceutical properties. In [...] Read more.
Vitis vinifera is an important fruit crop which is mainly consumed fresh or used for the production of wine. Genetic improvement programs through New Genomic Techniques (NGTs) aim to develop grapevine varieties resistant to biotic and abiotic stresses or enhancing nutraceutical properties. In order to apply NGTs, maintaining embryogenic calluses from flower tissues is critical. Optimizing culture conditions—pH, gelling agents, temperature, light, growth regulators, and gas composition—is essential for inducing efficient embryogenic responses tailored to each genotype/explant. Ethylene, a pivotal gaseous plant hormone, significantly influences tissue culture by affecting organogenesis and embryogenesis processes in several plants. Modulating ethylene levels shows promise for improving tissue culture vitality. This study evaluates in Vitis vinifera the effects of silver thiosulfate (STS) and salicylic acid (SA) on embryogenic callus growth, specifically investigating their roles in maintaining and inducing embryogenic competence. STS, particularly at 40 µM and 60 µM concentrations, effectively preserved embryogenic competence in Italia and Red Globe calluses, while high SA concentrations showed varied and occasionally adverse effects. At the same time, STS markedly suppressed the non-embryogenic callus growth in recalcitrant variety Italia, potentially increasing the ratio between embryogenic to non-embryogenic calluses development. Full article
(This article belongs to the Section Propagation and Seeds)
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11 pages, 2892 KB  
Article
Enhancing Red Table Grape Coloration Using Tsikoudia: A Novel and Sustainable Approach
by Emmanouil Kontaxakis, Dimitrios Lydakis and Ioannis Fisarakis
Plants 2024, 13(19), 2689; https://doi.org/10.3390/plants13192689 - 25 Sep 2024
Cited by 1 | Viewed by 1270
Abstract
Achieving optimal coloration in red table grapes, especially in warm-climate regions, presents significant challenges due to high temperatures that inhibit anthocyanin biosynthesis. Conventional methods to enhance grape coloration, including the use of abscisic acid (ABA), ethephon, foliar nutrient supplementation, and viticultural practices like [...] Read more.
Achieving optimal coloration in red table grapes, especially in warm-climate regions, presents significant challenges due to high temperatures that inhibit anthocyanin biosynthesis. Conventional methods to enhance grape coloration, including the use of abscisic acid (ABA), ethephon, foliar nutrient supplementation, and viticultural practices like cluster trimming and girdling, have limitations related to cost, regulatory restrictions, and potential adverse effects on grapes quality. This study proposes the application of tsikoudia, a traditional Greek alcoholic beverage, as a novel, sustainable, and cost-effective alternative to conventional practices. Tsikoudia, applied during the veraison stage, significantly improved the coloration of ‘Crimson Seedless’ and ‘Red Globe’ grapes by enhancing anthocyanin accumulation and altering color parameters. Specifically, lightness (L*), chroma (C*), and hue angle (h), measured using the CIE-Lab color system, were reduced, while the Color Index for Red Grapes (CIRG) was increased. Additionally, total anthocyanin content, determined through spectrophotometric analysis, also showed an increase. These changes indicate a more intense red coloration. This research highlights the effectiveness of tsikoudia in improving grape coloration and contributes to the development of more sustainable viticultural practices. Full article
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13 pages, 1220 KB  
Article
Phytosanitary Cold Treatment of the Spotted Wing Drosophila (Diptera: Drosophilidae) in Postharvest ‘Red Globe’ Grapes
by Tian-Bi Ma, Bo Liu, Yan Fang, Wen-Na Gao, Qing-Ying Zhao, Zhi-Hong Li and Guo-Ping Zhan
Horticulturae 2024, 10(8), 781; https://doi.org/10.3390/horticulturae10080781 - 24 Jul 2024
Viewed by 1339
Abstract
The spotted wing drosophila, Drosophila suzukii (Matsumura), is currently distributed in the main soft-skinned fruits production areas in China and 59 other countries, presenting a significant threat to importing nations. Optimal phytosanitary treatments, including fumigation, irradiation, and cold treatment, have been developed to [...] Read more.
The spotted wing drosophila, Drosophila suzukii (Matsumura), is currently distributed in the main soft-skinned fruits production areas in China and 59 other countries, presenting a significant threat to importing nations. Optimal phytosanitary treatments, including fumigation, irradiation, and cold treatment, have been developed to prevent the international movement of this invasive fly. To determine the most cold-tolerant stage and facilitate the development of the technical schedules requested by the Technical Panel on Phytosanitary Treatment (TPPT), cold treatments of D. suzukii immature stages in ‘Red Globe’ grapes were conducted. Dose–mortality data at 0 °C and 2 °C from repeated trials were subjected to analysis of covariance, linear regression, and probit analysis. Results identified 3 d old pupae as the most cold-tolerant stage, followed by 1 d old pupae, 4 d old larvae, and 6 h old eggs with similar tolerance. The 2 d old larvae were the most sensitive stage. In subsequent confirmatory tests, 3 d old pupae were subjected to cold treatment at 0 °C for 9 and 10 days, and at 2 °C for 10 and 12 days, based on the probit estimation of the probit-9 value. No adult emergence occurred in the confirmatory tests except for one deformed adult from a 2 °C 10 d treatment. Therefore, the recommended treatment schedule requires fruit temperatures below 0.00 °C (or 1.62 °C) for no less than 10 (or 12) continuous days, with treatment efficacy not less than 99.9960% (or 99.9955%) at a 95% confidence level, respectively. These schedules are intended for submission to TPPT for the development of phytosanitary treatment standards. Full article
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