Search Results (223,151)

Background: Bacterial keratitis, a major cause of corneal blindness, is frequently associated with biofilm-forming pathogens such as Klebsiella pneumoniae. Cyclic-di-GMP (c-di-GMP) controls biofilm development, which increases antibiotic resistance and makes treatment more difficult, highlighting the need for innovative therapeutic approaches. Methods: This study investigated cinnamaldehyde as a potential ocular therapeutic using combined computational and experimental approaches. Molecular docking and in vitro assays (XTT, resazurin reduction, crystal violet staining, qRT-PCR, and fluorescence microscopy) were used to evaluate the anti-biofilm and immunomodulatory activities of cinnamaldehyde (CA) against Klebsiella pneumoniae. Results: CA inhibited biofilm formation in a dose-dependent manner (≈89% at 1000 µM; >50% at 250 µM), reduced bacterial attachment to contact lenses, and downregulated key biofilm genes (mrkA, mrkC, ybtS, bolA). Docking analysis revealed strong binding affinity to the mrkH regulator (−5.46 kcal/mol. CA maintained more than 80% corneal cell viability by increasing IL-10, suppressing inflammatory mediators (IL-1β, IL-6, and TNF-α), and improving bacterial clearance. Conclusions: This study combines computational docking, biofilm quantification, immune cell assays, and functional gene expression analyses to reveal the ability of cinnamaldehyde not only to suppress biofilm formation but also to enhance macrophage-mediated clearance and modulate corneal immune responses, a multi-target approach not previously described in the context of bacterial keratitis. Such effects highlight its potential as a novel ocular drug candidate for protecting corneal integrity in infectious keratitis. Full article

Reinforced concrete shear wall structures (RCSWs) are commonly used as explosion-resistant chambers for storing hazardous chemical materials and housing high-pressure reaction equipment, serving to isolate blast waves and prevent chain reactions. In this study, full-scale experiments and numerical simulations were conducted to investigate the blast resistance of RC shear wall protective structures subjected to internal explosions. A full-scale RC shear wall structure measuring 9.7 m × 8 m × 6.95 m with a wall thickness of 0.8 m was constructed, and an internal detonation equivalent to 200 kg of TNT was initiated to simulate the extreme loading conditions that may occur in explosion control chambers. Based on experimental data analysis and numerical simulation results, the damage mechanisms and dynamic response characteristics of the structure were clarified. The results indicate that under internal explosions, severe damage first occurs at the wall–joint regions, primarily exhibiting through-thickness shear cracking near the supports. The structural damage process can be divided into two stages: local response and global response. Using validated finite element models, a parametric study was carried out to determine the influence of TNT charge weight and reinforcement configuration on the structural dynamic response. The findings of this research provide theoretical references for the design and strengthening of blast-resistant structures. Full article

To investigate the influence of segmental lining width on ground and tunnel deformation during shield tunneling beneath residential buildings, a numerical analysis model was established using Midas GTS NX based on the engineering context of the Guangzhou Metro Guanggang Xincheng depot tunnel underpassing residential structures. The simulation results were validated through comparison with field monitoring data, and a gray relational analysis was employed to quantitatively assess the sensitivity of various deformation indicators to segment width. The findings indicate that, under the engineering scenario of a shield tunnel crossing beneath residential buildings, the use of 1.2 m-wide segments is more effective in controlling ground settlement and structural deformation of the tunnel compared with 1.5 m-wide segments. The deformation process associated with the 1.2 m segments exhibits a more stable settlement pattern, whereas the 1.5 m segments tend to induce repeated settlement–heave cycles in the surrounding ground, with a potential risk of segmental displacement exceeding warning thresholds. Sensitivity analysis shows that different deformation indicators respond unevenly to changes in segment width. From most to least sensitive, the indicators rank as follows: maximum ground deformation, maximum displacement during the post-excavation stage, and maximum displacement during the excavation stage. The results of this study provide theoretical support and reference for selecting segmental lining width in shield tunnels constructed beneath residential buildings. Full article

We integrate morphological variation, CO1 distance and clustering, and geographic distribution to document unrecognized diversity within Meranoplus ‘berrimah’ and M. ‘snellingi’, members of the M. diversus group of specialist seed harvesters from Australia’s monsoonal (seasonal) tropics. This follows similar analyses of two other monsoonal ‘species’ of the group, M. ajax and M. unicolor, showing that both represent highly diverse complexes comprising an estimated 100 species each. We recognize eleven species among the 34 sequenced specimens attributable to M. berrimah and ten species among the 29 sequenced specimens attributable to M. snellingi. Images of all these species are provided. The M. berrimah complex has a far broader geographic range than was apparent when M. berrimah was originally described, occurring in the Kimberley region of Western Australia in addition to the Top End of the Northern Territory, whereas the M. snellingi complex appears to be restricted to the Top End. The limited geographic representation of our sequenced specimens suggests that many additional species occur in both complexes. We estimate that the M. snellingi complex contains 15–20 species in total, and that this number is considerably higher in the M. berrimah complex because of its broader distributional range. Our study provides further evidence that monsoonal Australia is a global centre of ant diversity, but it is not formally recognized as such because the great majority of its species is undescribed. Full article

Amidst the paradigm shift in park city development from quantitative metrics to spatial performance, urban complex parks—a novel green space type developed privately yet fulfilling public functions—present an innovative approach to park provision in high-density urban areas. However, systematic empirical evidence on their social value remains scarce. This study characterizes urban complex parks as a new form of green public space that provides key ecosystem services and proposes a three-dimensional evaluation framework integrating “usage vitality, place attractiveness, and user satisfaction.” Analyzing 19 park-equipped complexes among 75 cases in Shanghai using LBS data and online reviews through controlled linear regression and comparative analysis, our results indicate complexes with parks were associated with significantly outperforming others in place attractiveness and user satisfaction. Key findings include associations with a 413.7 m increase in average OD distance, a 3.4–4.0% higher city-level visitor share, and 5.24 percentage points greater median positive review rate. Crucially, spatial location outweighs green ratio and size in determining social value. Ground-level parks, through superior spatial integration, function as effective “social-ecological interfaces,” significantly outperforming rooftop parks in attracting long-distance visitors, stabilizing foot traffic (≈3% lower fluctuation), and enhancing per-store visitation. This demonstrates that green space quality (experiential quality and spatial configuration) matters more than quantity. Our findings suggest that urban complex parks can create social value through perceivable naturalness and restorative environments, providing an empirical basis for optimizing park city implementation in high-density contexts and highlighting the need to reconcile broad attractiveness with equitable local access. Full article

Old landslide reactivation poses a significant risk to infrastructure and settlements in mountainous regions. Its identification and accurate localization are crucial for mitigating reactivation hazards, yet are hindered by blurred morphological signatures and vegetation cover. This study develops a cross-regional workflow for the detection and GIS-based localization of old landslides using one-meter-resolution optical imagery and an enhanced YOLOv5 model. The workflow strictly separates training and detecting areas (Wanzhou for training, Zigui for detecting) to simulate realistic, unsurveyed scenarios. A Python script converts model outputs into shapefiles with precise geographic coordinates.. The results show an F1 score of 0.96 in the training area and 0.62 (mAP@0.5 = 0.58, Precision = 0.56, Recall = 0.67) in the detecting area. The analysis identifies causes of cross-regional performance degradation, including geomorphic confusion and potential detection of previously unmapped old landslides. These results demonstrate the feasibility of cross-regional landslide detection and highlight the potential of deep learning–GIS integration for practical hazard management. Full article

This study investigates the settlement characteristics of overlying strata in backfilled stopes at the Sanshandao Gold Mine, focusing on the compaction behavior of backfill materials. Integrating laboratory tests, numerical modeling, and field monitoring, we analyzed the particle size distribution and fractal dimensions of tailings (2.1525) and C material (2.1994), with tailings showing better gradation. Systematic compaction tests examined the effects of mix ratio, water content, and curing time. Results indicate that compression follows a viscous sliding model with exponential curves, progressing through three stages—pore compaction, structural deformation, and elastic/plastic deformation—with energy dissipation ratios of 1:5:18. Water content was the most influential factor, with optimal compaction occurring at 5~8%. Coupled Midas-Flac3D simulations estimated a theoretical compaction rate of 0~2% in filled stopes, excluding seepage and equipment effects. Field monitoring at the −480 m level revealed non-uniform settlement, with maximum subsidence of 63.75 mm above stopes and initial settlement rates of 12~20 mm/month. At the −520 m mining level, the backfill compaction rate reached 0.31%, with minor future increases expected. These findings offer valuable guidance for backfill mixture design and strata control in mining engineering. Full article

The problem of low water level and uneven distribution of flow in the multi-branch channels at the tail of the Ganjiang River (GJRT) during the dry season has been affecting the local water supply, navigation, and aquatic ecological environment. In recent years, water conservancy projects have been built in each branch of the multi-branch channels at the GJRT. Finding a way to utilize the water conservancy project group to carry out joint regulation and meet the water level and discharge requirements of each branch is an important issue that urgently needs to be solved. This paper analyzes the hydrodynamic process and its impact on water supply, navigation, and ecology in multi-branch channels without water conservation projects through hydrological data analysis and numerical simulation. By conducting numerical experiments on joint regulation of water conservation project group, a multi-objective regulation strategy is proposed to meet the water level and discharge of each branch. The results indicate that the discharge at the GJRT has been continuously decreasing from 1 September. Due to the jacking effect of Poyang Lake, the water level plunges at the GJRT from 1 October, which occurred later than the decrease in water level. The disruption of water levels and discharge makes it difficult to meet the regional water demand. The optimal time to initiate regulation is 1 October, and the target water level of Waizhou Station is 15.5 m, located upstream of the Ganjiang River tail. When the water level before each branch project gate is uniform and exceeds 15.5 m, the water level of Waizhou Station satisfies the requirement. However, the discharge of each branch does not meet the demand. In contrast to a scheduling regulation strategy that maintains the same water level in front of each gate, adopting a strategy with different water levels before each gate can effectively adjust the diversion ratio and fulfill the discharge demand of each branch at the tail of the Ganjiang River. Full article

The aim of the research was to detect the existing microclimatic conditions of the environment in the Visitor Center of the Sirmium Imperial Palace and to determine whether they pose any potential risks to the preservation of the mosaics in room 34 (M34). In order to estimate the microclimatic conditions of the environment and examine their effects on the deterioration processes of the mosaic, the following research methods were applied: one-year microclimatic monitoring of air temperature and relative humidity, monitoring of physical processes in the mosaic and on its surface, determining the presence of soluble salts, the potential biological contamination by aerobiological sampling, and the present biological contamination by using adhesive tape and sterile swabs. The results of microclimatic monitoring indicate that the relative humidity values during January, February, November and December were constantly above 80%. The annual range of temperature values ranged from 0.4 °C to 31.5 °C, while the relative humidity values ranged from 38.9% to 93.9%. The results of microbiological analysis showed high biological contamination of the M34 mosaic, which could be expected because the conditions were favorable for fungal growth throughout the year (a_w > 0.6). Soluble salts, i.e., sulfates, nitrates and chlorides, were identified on the mentioned mosaic. It can be concluded that the existing conditions in the Visitor Center of the Sirmium Imperial Palace pose a risk to the preservation of the mosaic and that they need to be improved. Considering the interdependence of the microclimatic conditions of the environment and the physical, chemical and biological processes of mosaic deterioration, microclimatic monitoring must be introduced at archeological sites with mosaics as a mandatory procedure for the purpose of monitoring the microclimatic conditions of the environment and preventive protection. Full article

Four thermomechanically rolled carbon steels with different strength levels and microstructures, namely S355M, X65M, S960M, and S1100M, were selected, and their critical hydrogen contents H_crit were determined. H_crit is the hydrogen content where brittle fracture of the steel can occur. The H_crit values for the four carbon steels are 2.03 (S355M), 0.91 (X65M), 0.32 (S960M), and 0.53 ppm (S1100M). Bainitic carbon steel S1100M outperforms lower-strength tempered martensitic steel S960M in terms of resistance to hydrogen embrittlement. All other steels follow the trend that a higher strength of steel results in a lower resistance to hydrogen embrittlement. The root cause for the beneficial behavior of bainitic steels is discussed. Full article

Alzheimer’s disease (AD) is marked by cognitive decline, and also by retinal degeneration. Having in mind that docosahexaenoic acid (DHA, 22:6n − 3) is a safe, low-cost, and pivotal fatty acid for brain health and sustained cognitive function, this study exploits environmentally friendly non-fish sources as potential dietary supplements enriched with DHA to prevent or reverse AD. Forty 5xFAD transgenic male mice, aged five weeks old, were randomly distributed by five body weight-matched dietary groups (with eight animals each) and fed isocaloric diets based on the AIN-93M standard formulation for rodents for 6 months. Except for the control feed (without supplementation), each diet contained a modified lipidic fraction supplemented with 2% of the following: (1) linseed oil (LSO, rich in alpha-linolenic acid (ALA, 18:3n − 3)); (2) cod liver oil (fish oil, FO, rich in both DHA and eicosapentaenoic acid (EPA, 20:5n − 3)); (3) Schizochytrium sp. microalga oil (Schizo, with 40% of DHA); and (4) commercial DHASCO (DHASCO, with 70% of DHA). The aim of this study was to measure retinal neural layer thickness, calculate ganglion cell layer (GCL) density, and assess retinal injury by means of immunohistochemical staining for β-amyloid plaques deposition, TAU protein levels, and IBA1, as hallmark features of AD progression, in order to elucidate the effects of different dietary DHA treatments in Alzheimer’s retinas. Although no statistical differences were observed across retinal layer thicknesses depending on the diet (p > 0.05), there was a consistent pattern for slightly increased retinal thickness in 5xFAD mice fed fish oil relative to the others for the measurement of total layers, in general and for the inner segment/outer segment layer, the outer nuclear layer, the outer plexiform layer, the inner nuclear layer, and the inner plexiform layer, in particular. The ganglion cell layer (GCL) density was increased in 5xFAD mice fed the DHASCO oil diet relative to the control (p < 0.05), suggesting a benefit of DHA supplementation on the number of viable ganglion cells. No positive staining was observed for β-amyloid plaques deposition or the neuroinflammatory marker, IBA1, corroborating previous findings in human AD retinas. Conversely, the internal retinal layers showed intense TAU immunostaining. Immnunostained TAU area was significantly reduced in 5xFAD mice fed a fish oil diet compared to control (p < 0.05), although the number of TAU-positive cells did not differ across diets (p > 0.05). The retinal protected integrity derived from the benefits of DHA supplementation found, either from fish oil or DHASCO oil, underscores the potential of retinal biomarkers as non-invasive indicators of cognitive decline and overall brain health, opening new avenues for investigating AD pathophysiology in the retina. Full article

This research highlights the mechanical performance of alkali-activated polypropylene fiber (PPF) mortar containing natural pozzolan (NP) and waste glass powder (WGP) as partial replacements for cement. A total of 45 mix combinations and 405 samples were prepared by varying the levels of NP, WGP, PPF, and sodium silicate (SS), with sodium hydroxide (SH) as an alkali activator. The levels for these variables are NP (0%, 10%, and 20%) and WGP (0%, 10%, 20%, and 30%) by weight of the cement; PPF (0%, 0.5%, and 1.5%) by volume of mortar; and SS + SH (30%, 40%, and 50%) by weight of the binder. The molarity of the SH solution was kept at 10 M, while the SS/SH ratio was maintained at 2.5. Compressive (f’c), flexural (fr), and split tensile strength (ft) were evaluated at 7, 28, and 90 days. The results showed that strength development is strongly age-dependent, with 85–90% of the total strength achieved at 28 days and continued moderate gains to 90 days. SS + SH was the most significant variable, with 50% of activator content achieving the highest f’c, fr, and ft values. Within the tested ranges of NP (0–20%) and WGP (0–30%), strength showed a decreasing trend with increasing replacement due to dilution. PPF had a very minute effect on f’c but significantly improved fr and ft at 0.5% dosage because of crack-bridging. Correlation analysis confirmed that cement and SS + SH are the most dominant strength-controlling factors. The results suggest that the combined use of NP, WGP, and PPF maintains mechanical performance while reducing cement consumption, highlighting the feasibility of this hybrid alkali-activated mortar as a low-carbon construction material. Full article

The giant freshwater prawn Macrobrachium rosenbergii is a globally farmed decapod species of high economic and nutritional value, but its genetic improvement has been constrained by the lack of an efficient genome editing workflow. Here, we optimized a CRISPR/Cas9 microinjection system for targeted mutagenesis in M. rosenbergii embryos. A controlled mating-based approach enabled routine collection of one-cell embryos, and developmental staging identified 0.5–2 h post-fertilization as the optimal injection window. Optimization of the needle tip (~1 μm) minimized mechanical damage, lowering early embryo mortality to ~10%. Using this system, the eye-development gene MrPAX6 and the sex-related gene MrIAG were successfully edited. MrPAX6 editing produced a 46.9% mutation rate and an average 50% reduction in eye pigment area. MrIAG editing achieved an 84% mutation rate, while post-injection survival to hatching was approximately 35%. These results establish an efficient and reproducible CRISPR/Cas9 editing system in M. rosenbergii, providing a foundation for functional genomics and genetic improvement in this commercially important prawn species. Full article

Background: Excess accumulation of white adipose tissue is linked to the development of obesity and type 2 diabetes, both of which are associated with systemic metabolic dysfunction. One promising approach is to convert white adipocytes into beige adipocytes, which have greater thermogenic potential and improved insulin sensitivity. Trans-resveratrol (RES), a polyphenolic compound known to have multiple metabolic benefits, has been reported to promote browning of adipocytes and improve insulin signaling; however, it is unclear whether sex and diabetic status modify RES’s effects. Methods: We evaluated the ability of RES to induce browning and increase insulin sensitivity in adipose-derived stromal cells (ADSCs) derived from diabetic db/db mice and explored the extent to which these responses are modulated by sex and diabetic status. Subcutaneous ADSCs were isolated from wildtype (WT) and diabetic (db/db) male and female mice and then treated with RES during beige adipocyte differentiation. Results: RES enhanced the expression of Pgc1α and Ucp1 mRNA and increased mitochondrial proton leak in ADSCs of both WT and db/db mice. RES also enhanced insulin-induced AKT phosphorylation in all groups of ADSCs. Notably, the effects of RES on browning and insulin signaling were influenced by the sex and diabetic status of the mice, as ADSCs from female diabetic mice responded differently compared with those from their WT or male counterparts. Conclusions: These findings highlight the importance of considering sex and diabetic status when developing browning strategies to target obesity and type 2 diabetes. Full article

Microalgae are increasingly recognized as promising biostimulants for sustainable agriculture, yet their potential remains underexplored due to the complexity of biostimulant activity and the vast diversity of species. Efficient standardized screening approaches are therefore needed. In this study, a high-throughput screening platform assessed the biostimulant activity of five microalgal species (Limnospira platensis, Chlorella vulgaris, Dunaliella salina, Microchloropsis gaditana, and Isochrysis galbana) in Arabidopsis thaliana. The system enabled full life-cycle assessment of A. thaliana under optimal and drought stress conditions, incorporating three application methods (soil amendment, irrigation, foliar spray) and a wide concentration range of 0.01–0.5 g/L. Biostimulant efficacy depended strongly on concentration and application method. Irrigation-based applications generally enhanced drought tolerance but delayed bolting and flowering. The highest concentration inhibited germination and root elongation, likely due to bioactive compound toxicity rather than salinity or pH. L. platensis exhibited broad activity across environmental conditions, while I. galbana likewise showed wide-ranging effects, including enhanced generative growth. In contrast, D. salina and M. gaditana primarily improved drought tolerance, and C. vulgaris acted mainly under optimal conditions. These findings highlight the value of A. thaliana to accommodate rapid biostimulant screening and identify both novel and established microalgae for further validation in crops. Full article