Search Results (85)

Laurus nobilis L. (Lauraceae), widely recognized as bay laurel, has long held prominence in traditional medicine across diverse cultures and has attracted growing attention within contemporary pharmacological research. This review endeavors to synthesize and critically evaluate evidence from in vitro, in vivo, and clinical studies to elucidate the therapeutic potential of this species, an aromatic evergreen shrub native to the Mediterranean region. Its leaves and essential oil have long been utilized in culinary and traditional medicine, and recent scientific studies have revealed a wide range of pharmacological properties. A comprehensive literature search was conducted using the keyword “Laurus nobilis L.” across Scopus, PubMed, and Google Scholar databases. The search included English-language resources published up to 31 January 2025, focusing on in vitro, in vivo, and human studies. Exclusion criteria encompassed letters, conference proceedings, and articles related to agriculture, genetics, nursing, environmental and veterinary sciences, multidisciplinary studies, and non-medical or pharmaceutical themes. The findings indicate that L. nobilis exhibits diverse pharmacological activities, including wound-healing, dental plaque-reduction, hepatoprotective and gastroprotective effects, antidiabetic activity, antioxidant and anti-inflammatory properties, antimicrobial and antiparasitic effects, neuroprotective potential, antigenotoxic and anti-hypersensitivity actions, and renal protection. This review manuscript seeks to further illuminate the pharmacological potential of Laurus nobilis while articulating the biophysical underpinnings and mechanistic coherence through which its bioactive constituents manifest their therapeutic effects. In conclusion, L. nobilis demonstrates significant promise as a natural, effective, and safe therapeutic candidate for various health conditions. Nonetheless, further clinical research is warranted to substantiate its efficacy and safety, thereby enabling its integration into evidence-based medical practice. Full article

The essential oil (EO) industry generates large volumes of solid distillation residues that are commonly discarded as waste, despite their potential as a source of nutrients and bioactive compounds. This study evaluated the chemical quality and the volatile profile of four types of exhausted biomasses (EBs) obtained after steam distillation of EOs from bay laurel (Laurus nobilis), lavender (Lavandula angustifolia), lavandin (L. angustifolia × L. latifolia) and two industrial hemp specimens (Carifit1p, a breeding line, and Codimono, a cultivar), with a view to their potential valorisation in animal feeding. The nutritional composition of the distillation residues was determined according to AOAC methods, while the headspace volatile fraction was characterised by HS-SPME–GC/MS. The EBs were generally characterised by a high fibre content, with the highest crude fibre and NDF values in whole lavender and lavandin plants, whereas the industrial hemp line Carifit1p showed the highest crude protein (17.1% DM) and ash contents. The volatile profiles of the residues differed markedly from those typical of the corresponding fresh EOs: the bay laurel residue was dominated by monoterpene hydrocarbons (74.5%) with strongly depleted 1,8-cineole (1.9%), while lavender and lavandin retained high levels of β-caryophyllene (35.8% and 32.4%, respectively). Industrial hemp residues were enriched in oxygenated sesquiterpenes, with caryophyllene oxide reaching 21.2% in Carifit1p and exceeding β-caryophyllene. Overall, the results indicate that EO exhausted biomasses retain a species-specific profile of fibre, residual nutrients and less volatile, less water-soluble terpenoids, supporting their potential reuse as feed ingredients and contributing to a more sustainable, near-zero-waste distillation supply chain. Full article

Bay laurel (Laurus nobilis L., Lauraceae) is an important Mediterranean aromatic species widely used as a culinary spice and a source of bioactive constituents. This study aimed to evaluate the essential oil (EO) composition, macronutrient content, polyphenolic profile and antioxidant activity of bay laurel along the Croatian Adriatic coast. A total of 49 volatile compounds were identified in dried leaves using GC–MS, representing 99.27–100.00% of the EO composition. The EOs were dominated by oxygenated monoterpenes (86.51–95.55%), with 1,8-cineole (42.40–56.83%), α-terpinyl acetate (6.89–12.76%), sabinene (6.10–8.65%), linalool (2.06–14.46%), and methyl eugenol (0.45–10.10%) as the major constituents. Polyphenolic profile obtained by spectrophotometric methods showed that total polyphenol content ranged from 6.03% to 9.65%, tannins from 1.81% to 4.76%, flavonoids from 0.23% to 0.71%, and total phenolic acids from 1.34% to 3.03% (rosmarinic acid equivalents) and 2.51% to 5.66% (chlorogenic acid equivalents). Furthermore, we highlighted the antioxidant activity of Croatian bay laurel using the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays. Ethanolic extracts exhibited higher antioxidant activity than aqueous extracts, reaching 74.58–82.03% ABTS inhibition and 57.93–69.54% DPPH inhibition. This study provides the first integrated characterization of Croatian bay laurel leaves and highlights their antioxidant potential. Full article

The valorization of medicinal and aromatic plant by-products represents a sustainable strategy to recover bioactive compounds and reduce agricultural waste. In this study, pruning residues of Laurus nobilis L. and Myrtus communis L. were investigated as sources of essential oils (EOs) for bio-based weed management. Leaves and young stems were subjected to anatomical, micromorphological, and histochemical analyses to ascertain that active secretory structures were maintained. EOs were then obtained by steam distillation and characterized by GC-FID and GC-MS. Phytotoxicity was evaluated on two crop species, Hordeum vulgare L. and Raphanus sativus L., and two weed species, Lolium multiflorum Lam. and Sinapis alba L.; ecotoxicity was assessed using Artemia salina and Daphnia magna. The EOs were mainly composed of oxygenated monoterpenes, with 1,8-cineole as the dominant constituent in both oils. Phytotoxicity was species- and concentration-dependent, with M. communis EO showing the strongest inhibition, particularly against S. alba. Ecotoxicological assays revealed limited toxicity toward A. salina, but concentration-dependent immobilization of D. magna. Overall, these findings support the circular valorization of laurel and myrtle pruning residues as sources of bioactive EOs while highlighting the need for further formulation, selectivity, and environmental safety studies before agricultural application. Full article

Bay laurel (Laurus nobilis L.) leaves and branches represent promising lignocellulosic residues for biorefinery and bioenergy applications. The present study evaluated the effect of autohydrolysis at different temperatures and residence times on the chemical composition, structural properties, and higher heating value (HHV) of both biomass fractions. The initial characterization revealed that leaves were richer in extractives and lignin, whereas branches contained higher amounts of α-cellulose and hemicelluloses. Autohydrolysis promoted the selective solubilization of biomass components, reaching maximum values of approximately 38% for leaves and 30% for branches. Increasing treatment severity enhanced hemicellulose removal and resulted in a relative enrichment of lignin and cellulose in the solid residues, while FTIR analysis showed that the main lignocellulosic structure was largely preserved. The solid residue after polyalcohol liquefaction presented a higher heating value, improving the heating value of the resulting solids, achieving maximum HHVs of 30.08 MJ kg⁻¹ for leaves and 29.46 MJ kg⁻¹ for branches at 180 °C for 30 min. Overall, the results indicate that autohydrolysis is a suitable strategy for the selective extraction of hemicellulose-rich fractions and the production of lignin-enriched solid residues. This process contributes to the sustainable valorization of bay laurel biomass within an integrated biorefinery framework. Full article

Laurel (Laurus nobilis L.) is a characteristic species of the Mediterranean flora, valued for its medicinal, aromatic, and culinary uses. Many of these properties are attributed to its volatile constituents. In this study, the effect of Atmospheric Room Temperature Plasma (ARTP) pretreatment on laurel powder was evaluated, with emphasis on volatile recovery and food application. Volatile extraction was optimized using headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography–mass spectrometry (GC-MS), investigating key parameters such as salt concentration, extraction temperature, equilibration time, extraction time, and fiber type. Subsequently, critical ARTP variables (nitrogen flow, treatment duration, treatment distance, substrate thickness, and plasma power) were optimized. Response Surface Methodology was applied in both optimization processes. The results demonstrated that fiber type was the most influential factor for volatile recovery, with extraction temperature also exerting a significant effect. A more nuanced pattern emerged during ARTP pretreatment, where moderate plasma intensities enhanced the recovery of several key volatiles. This trend was not uniform across compounds, indicating that plasma-induced microstructural changes interact with the physicochemical properties of individual analytes. To demonstrate food relevance, a ready-to-use broth model prepared with laurel powder confirmed improved headspace transfer of characteristic volatiles following ARTP treatment. Taken together, these findings suggest that ARTP can serve as a practical, non-thermal pretreatment for improving volatile release, supporting its potential use in the food, pharmaceutical, and cosmetic industries. Full article

Laural (Laurus nobilis) is a Mediterranean plant with reported antibacterial properties, yet the genetic basis of its antibacterial efficacy remains largely unexplored. This study evaluated the antibacterial activity of Laurus nobilis methanolic extracts against Escherichia coli, Staphylococcus aureus, and Bacillus cereus, combined with genome-wide association studies (GWAS) and machine learning (ML) approaches to identify genetic markers and predict antibacterial efficacy in 92 plant samples. Antibacterial tests revealed significant variability in inhibition zones, with E. coli showing the highest inhibition (Canakkale2: 24.5 mm), followed by S. aureus (Aydin2: 26.0 mm). Minimum inhibitory concentration (MIC) analysis demonstrated notable regional differences; extracts from Mersin3 showed the highest efficacy (MIC = 6.25 mg/mL), while Aydin1 exhibited the lowest activity (MIC = 100 mg/mL). Population structure and neighbor joining tree analysis split the germplasm into two groups. GWAS identified significant genetic markers associated with antibacterial traits, including marker 26557159 for EC-MEAN (Escherichia coli-Mean) (p = 1.10 × 10⁻⁴, MarkerR² = 0.1799, genetic variance = 9.41792) and marker 26584774 for BC-MEAN (Bacillus cereus-Mean) (p = 8.89 × 10⁻⁵, MarkerR² = 0.18512, genetic variance = 12.48948). Protein–protein interaction network of loci associated with marker trait association (MTA) marker (26557159) indicated involvement in high-affinity secondary active ammonium transmembrane transporter activity, providing insights into genetic regions influencing antibacterial properties. ML models predicted antibacterial activity with high accuracy. XGBoost achieved the best performance for MIC predictions (R² = 0.999, RMSE = 0.434), while random forest (R² = 0.984) demonstrated robust performance for both MIC and disc diffusion assays. LightGBM performed well for MIC prediction (R² = 0.988) but showed limited accuracy for disc diffusion outcomes (R² = 0.695). This study is the first to combine GWAS and ML for predicting antibacterial efficacy in L. nobilis, identifying specific genetic markers (e.g., 26557159, 26584774) and demonstrating that XGBoost achieves near-perfect MIC prediction (R² = 0.999). These findings provide a genomic and computational foundation for marker-assisted breeding of laurel with enhanced antibacterial properties and support the sustainable use of plant-derived anti-microbials. Full article

Climate change poses significant threats to Mediterranean plant species, including Laurus nobilis L., an ecologically and economically important tree. This study evaluates potential shifts in its suitable distribution areas across Türkiye under future climate scenarios [Shared Socioeconomic Pathway 2-4.5 (SSP2-4.5) and 5-8.5 (SSP5-8.5)] using an ensemble species distribution model incorporating ten algorithms. Key environmental drivers—elevation, annual mean temperature (Bio1), and evaporation including sublimation and transpiration (evspsbl)—were identified as critical factors influencing habitat suitability. Results indicate substantial spatial redistributions, with habitat losses projected in inland transition zones toward continental climates, particularly in parts of the Aegean and Black Sea regions. The current suitable distribution area across the country, approximately 18.48%, could rise to 18.55% by 2040 under the SSP2-4.5 scenario and to 18.76% by 2060 under the SSP5-8.5 scenario. However, without human intervention, the species’ establishment in these new suitable distribution areas is not considered possible. Moreover, it has been determined that the suitable distribution area of the species could decrease to 17.48% by 2060 under the SSP2-4.5 scenario and to 17.31% by 2080 under the SSP5-85 scenario. This result indicates that there could be a loss of more than 8% of the suitable distribution area between 2060 and 2080, according to the SSP5-8.5 scenario. Conversely, limited expansions may occur in specific areas, including the northern Aegean and the Hatay-Antep region. By 2100, despite periodic fluctuations, a net decline in suitable habitats is expected under both scenarios. Notably, spatial analysis reveals that while some newly suitable areas may emerge, natural migration will likely be insufficient for population persistence, necessitating human-assisted adaptation strategies. These findings underscore the need for proactive conservation measures, such as identifying climate-resilient provenances, assisted migration, and targeted reforestation in future suitable zones. Given that most Turkish forests are state-managed, collaboration with the General Directorate of Forestry is essential to integrate climate adaptation into long-term management plans. This study provides a framework for mitigating climate-induced habitat loss in L. nobilis while offering insights applicable to other vulnerable Mediterranean species facing similar threats. Full article

Dietary phytogenic additives and microalgae are increasingly investigated as natural alternatives to antibiotic growth promoters in rabbit production due to their potential effects on gut health and nutrient utilisation. This study evaluated the nutraceutical potential of Chlorella vulgaris and Laurus nobilis as plant-based additives for growing New Zealand White rabbits. A 45-day feeding trial was conducted using control and experimental diets enriched with 0.1% Chlorella and 0.1% Laurus. Productive performance, nutrient digestibility, blood biochemistry and faecal composition were monitored, and polyphenolic compounds were analysed in feed, blood, faeces and caecal microbiota using HPLC-DAD. Final body weight (3097 vs. 2909 g) and feed intake (142.7 vs. 145.0 g day⁻¹) did not differ significantly between treatments. However, crude protein digestibility was significantly lower in the supplemented group than in the control group (54.39–47.79% vs. 63.73–62.33%; p < 0.05). Faecal chemical composition differed significantly between groups, particularly for dry matter, which was higher in the supplemented group across sampling times. Polyphenols detected across biological matrices confirmed the bioavailability of selected phytochemicals, with ferulic acid showing the highest stability. Correlation analysis indicated shared metabolic or absorptive pathways among several compounds. Overall, low-dose supplementation with C. vulgaris and L. nobilis appears safe and may support improved digestive physiology and nutrient utilisation without compromising rabbit health. Further research with larger sample sizes and detailed microbiome profiling is needed to clarify metabolic interactions and long-term effects of these nutraceutical strategies. Full article

Seasonal variation is recognized as a key factor affecting the essential oil (EO) yield, chemical composition, and antioxidant activity of Laurus nobilis L. from the Montenegro coast, which constituted the focus of this research. The bay essential oil (BEO) yield was higher in summer (2.12%) and autumn (2.03%) than in winter (1.26%) and spring (1.28%). The total number of BEO components, depending on seasonal variability, ranges from 31 (summer) to 34 (winter and spring). 1,8-cineole (eucalyptol) was the major aromatic compound in all seasons, with the highest content recorded in summer (52.4%). Linalool, as the second most abundant component, is present in the autumn harvest (14.1%), while α-terpinyl acetate, as the third most abundant component, is most prevalent in the winter–spring period (9.6–9.7%). Two groups of monoterpenes, namely the oxygen-containing monoterpene derivatives (80.1%), constitute the most abundant components in BEO leaves, as well as monoterpene hydrocarbons (14.3–15.5%). The phenylpropanoids were the least abundant (4.9–6.3%). Stronger antioxidant activity (DPPH) during an incubation time of 20 min was shown by the BEOs isolated from autumn (EC50 value of 1.15 mg/mL). Early autumn (September) represents the optimal harvest time for L. nobilis in Montenegro, as they ensure a high essential oil yield and better quality, reflected in a high 1,8-cineole content and stronger antioxidant activity of the oil. These results demonstrate that seasonal variations are key factors regulating the quantity and quality of BEO, providing important information for optimizing harvest strategies for medicinal and industrial purposes. Full article

Laurus nobilis hydrosol (HyLN), a water-soluble byproduct of essential oil extraction, containing beneficial antioxidants and antimicrobial compounds, was used as a sustainable ingredient in the development of a natural antioxidant-rich hydrogel formulation. Hydrogels were formulated using sodium hyaluronate and xanthan gum, natural ingredients with beneficial effects on the skin, while β-cyclodextrin (βCD) was added to enhance the stability of antioxidants in HyLN. Extensive rheological and textural analyses were employed to optimize the hydrogel formulation for dermal administration, while stability studies assessed the chemical and physical stability of developed formulations. A combination of sodium hyaluronate and xanthan gum provided several HyLN hydrogel formulations with tunable rheological and textural properties, presenting adequate physical and microbiological stability over 6 months of storage. The use of βCD failed to stabilize inherently unstable antioxidants in HyLN hydrogels, yet their residual antioxidant activity remained notable. An in vitro scratch test using a human keratinocyte cell line showed that the developed HyLN gel does not interfere with wound healing. HyLN hydrogels showed a pronounced occlusive effect in vitro, reaching up to 80% of that measured for Vaseline, which helps maintain skin hydration and appearance. Full article

Hepatocellular injury syndrome represents a pathological process with a broad etiological spectrum, including viral infections, autoimmune diseases, or intoxications. Clinicians must identify the potential cause using both anamnestic data and available paraclinical examinations. We present the case of a 55-year-old female patient, admitted to the Internal Medicine 1 Department at the Clinical County Emergency Hospital Bihor, Oradea, Romania. The patient exhibited nonspecific complaints and insignificant pathological antecedents, but from a biochemical perspective, substantial changes in liver transaminase levels were evident. To establish differential diagnoses, a series of biochemical and immunological tests were performed, along with a thorough medical history. It was concluded that the patient regularly consumes herbal infusions, specifically Laurus nobilis leaves, commonly known as Bay Laurel. Although this might be easily overlooked at first glance, a closer examination could explain the current clinical picture. In April 2024, a 55-year-old female patient with no history of liver pathology was admitted. She complained of asthenia fatigue, anorexia, mixed dyspeptic symptoms, diffuse abdominal pain, and a weight loss of 12 kg. The pathology had insidiously started approximately 3 months prior. On examination, the patient had altered general status, anorexia, and was overweight. Biochemically, the patient had elevated liver transaminase values (AST = 196 U/L and ALT = 357 U/L) that continued to rise during hospitalization, despite hepatoprotective treatment. Various paraclinical examinations were performed to exclude other potential causes of hepatic aggression, having excluded ordinary causes. Consequently, a liver biopsy was performed, and the histopathological examination leaned toward a toxic hepatitis etiology. Application of the updated RUCAM scale yielded a score of eight points (“probable” HILI—Herb-Induced Liver Injury). Clinical and biochemical improvement was observed after complete cessation of bay leaf tea consumption. This case highlights the potential hepatotoxicity of commonly used culinary herbs when consumed in large quantities or as concentrated infusions and emphasizes the importance of detailed anamnesis regarding herbal product use. Full article

Essential oils (EOs) from aromatic plants are valuable sources of bioactive compounds with potential applications as natural antimicrobials and antioxidants. This study investigated the chemical composition, antimicrobial and antioxidant activities, and in silico pharmacological properties of EOs extracted from Laurus nobilis, Thymbra capitata, and Mentha piperita, three medicinal species traditionally used in northern Morocco. Hydrodistillation yielded 1.12–1.53% oils, and GC–MS analysis revealed distinct chemotypes: L. nobilis was rich in eucalyptol, linalool, methyleugenol, and α-terpinyl acetate; T. capitata was dominated by carvacrol (80.95%), and M. piperita contained high proportions of linalool (57.37%) and linalyl acetate (28.56%). Antimicrobial assays demonstrated strong activity of all oils against Gram-positive and Gram-negative bacteria as well as Candida species, with T. capitata showing the most potent and broad-spectrum effects (MIC 0.073–9.47 mg/mL), attributed to its high carvacrol content. Antioxidant assays (DPPH and ferric reducing power) identified L. nobilis as the most active radical scavenger (IC₅₀ = 0.037 mg/mL), followed by T. capitata, whereas M. piperita displayed weaker activity. PCA confirmed that phenolic monoterpenes drive both antimicrobial and antioxidant potency, while oxygenated terpenes and sesquiterpenes contribute more selectively. ADMET predictions indicated generally favorable absorption and low toxicity, although o-cymene and carvacrol presented potential safety concerns. Molecular docking revealed α-terpinyl acetate as the most versatile ligand, with strong binding to bacterial, fungal, and oxidative enzymes, whereas other compounds exhibited more selective affinities. Collectively, these findings highlight the potential of Moroccan EOs, particularly T. capitata and L. nobilis, as promising natural alternatives to synthetic antimicrobial and antioxidant agents. Full article

This study explores the anticancer, antioxidant, and phytochemical activities of Laurus nobilis L. ethanolic leaf extract. The extract demonstrated selective cytotoxicity against four human cancer cell lines, showing strong cytotoxic effect against ovarian (ES2), head and neck (SAS), and colorectal (HT-29) cancer cells, with IC₅₀ values ranging from 3.8 ± 0.3 to 4.4 ± 0.6 µg/mL. Notably, it exhibited only moderate inhibition of the MDA-MB-231 breast cancer cell line (IC₅₀ = 18.5 ± 0.8 µg/mL), possibly reflecting intrinsic differences in cell line sensitivity. Importantly, the extract showed low toxicity toward normal human fibroblasts (HDF), with an IC₅₀ value exceeding 100 µg/mL, indicating a favorable selectivity profile. The flow cytometry analysis showed that the extract caused cell death and stopped the cell cycle in both SAS and ES2 cancer cell lines. In SAS cells, extract treatment significantly increased apoptotic cells (21.1% ± 0.3%) compared to the control (6.3% ± 0.4%), along with G2 phase accumulation, indicating G2 arrest. Similarly, in ES2 cells, apoptosis increased (16.2% ± 1.3% vs. control 8.1% ± 1.0%), and a significant cell accumulation in the S phase was observed, suggesting disruption of cell cycle progression. Antioxidant screenings showed impressive dose-dependent DPPH radical scavenging activity (25–2000 µg/mL), although less potent than ascorbic acid (2.6 µg/mL). UPLC-QTOF/MS phytochemical analysis revealed various phenolic constituents, such as flavonoids and phenolic acids, and an inferred association with the recorded bioactivities. This preliminary work indicates that L. nobilis extracts may act as natural anticancer and antioxidant agents; however, it was limited to in vitro testing with non-standardized samples, underscoring the need for further research to validate and extend these findings for future applications. Full article

Anesthesia has emerged as a critical strategy for maintaining fish viability during transport, with natural anesthetics gaining increasing attention in recent research. The active ingredients in Laurus nobilis L. have antioxidant effects and reduce cell apoptosis. Studies have shown that they can upregulate expression of Nrf2 in mitochondrial biosynthetic factors. This study aimed to investigate the effects of laurel (Laurus nobilis) essential oil on oxidative stress and apoptosis mechanisms during the live transport of hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂). The addition of laurel essential oil during transport activated the Nrf2-Keap1 antioxidant pathway, resulting in up-regulated expression of catalase (cat) and superoxide dismutase (sod) genes. This led to increased enzymatic activity and reduced levels of oxidative stress markers. The mitigation of oxidative stress contributed to physiological stability by downregulating apoptotic gene expression (Bax, Caspase 8), reducing gill and liver tissue damage, and lowering the activity of hepatocyte damage markers aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Metabolomic analysis revealed several key metabolic pathways affected during transport, with the FoxO signaling pathway demonstrating the most significant impact. Within this pathway, reduced glutamate levels appeared to inhibit apoptosis, while decreased ADP and AMP levels potentially influenced antioxidant capacity. The addition of laurel essential oil to transport water proved beneficial in reducing biochemical markers of stress responses in hybrid grouper during keep live transport. Full article