Search Results (2,572)

To investigate the influence of segmental lining width on ground and tunnel deformation during shield tunneling beneath residential buildings, a numerical analysis model was established using Midas GTS NX based on the engineering context of the Guangzhou Metro Guanggang Xincheng depot tunnel underpassing residential structures. The simulation results were validated through comparison with field monitoring data, and a gray relational analysis was employed to quantitatively assess the sensitivity of various deformation indicators to segment width. The findings indicate that, under the engineering scenario of a shield tunnel crossing beneath residential buildings, the use of 1.2 m-wide segments is more effective in controlling ground settlement and structural deformation of the tunnel compared with 1.5 m-wide segments. The deformation process associated with the 1.2 m segments exhibits a more stable settlement pattern, whereas the 1.5 m segments tend to induce repeated settlement–heave cycles in the surrounding ground, with a potential risk of segmental displacement exceeding warning thresholds. Sensitivity analysis shows that different deformation indicators respond unevenly to changes in segment width. From most to least sensitive, the indicators rank as follows: maximum ground deformation, maximum displacement during the post-excavation stage, and maximum displacement during the excavation stage. The results of this study provide theoretical support and reference for selecting segmental lining width in shield tunnels constructed beneath residential buildings. Full article

The Arctic is warming three to four times faster than the global average. This is transforming global maritime routes, thereby increasing shipping and resource extraction in Alaska. This surge requires sustainable energy solutions as policy trends towards stricter emissions standards. This article assesses the potential of Geothermal-to-X (GtX) technologies in establishing clean refueling infrastructure across Alaska, using its untapped geothermal resources. GtX uses electrolysis to split water into hydrogen and oxygen, a process powered by geothermal energy. Hydrogen and its X products, such as green methane or green ammonia, can be stored as fuels and are largely recognized as the key to a carbon-free future to address the growing energy demand. This study assesses the technical, economic, strategic, and geological feasibility of GtX refueling hubs in Alaska. Five locations were denoted as potential candidates and beckon future research. This study concludes that Unalaska is the most viable initial GtX hub given the highest Multi Criteria Decision Analysis (MCDA) score from its combination of a high-quality geothermal resource, an existing and accessible deepwater port, and a sizable local energy demand. The goal of this study is to provide an accessible and comprehensive resource for stakeholders and policymakers, outlining an energy future with sustainable maritime development, powered by affordable and secure energy. Full article

A five-month-old, intact, female Miniature Schnauzer Terrier presented with persistent severe hemolytic anemia following an initial infection with Babesia gibsoni and B. vogeli. Despite treatment, severe regenerative anemia persisted, and the patient was unresponsive to antibiotics, as well as antiprotozoal and immunosuppressive agents. Subsequent laboratory tests and diagnostic imaging ruled out persistent hemiparasitic infections, immune-mediated diseases, or neoplasia. Genomic DNA and cDNA sequencing identified a point mutation in exon 8 (g.4978G>T) that introduced a premature termination codon, leading to exon 8 skipping and a single-nucleotide deletion at the exon 7–intron 7 boundary (c.966delG) during splicing. A 151 bp deletion in the coding region of the patient’s PKLR cDNA was subsequently detected, which ultimately resulted in pyruvate kinase deficiency. This missplicing results in a premature stop codon and disrupts PKLR tetramer formation owing to the partial loss of domain A and complete loss of domain C. Enzyme activity assays confirmed a complete loss of function in the mutant PKLR protein compared to the wild-type, supporting the causal role of this deletion in non-spherocytic hemolytic anemia. This is the first report as per our knowledge documenting truncated PKLR variant in a dog, and notably, the first such case in a Miniature Schnauzer breed. Full article

Hereditary Hemorrhagic Telangiectasia (HHT), also known as Rendu–Osler–Weber syndrome, is a disorder of angiogenesis characterized by mucocutaneous telangiectasias and visceral arteriovenous malformations. This rare autosomal dominant disorder is caused by pathogenic variants in the ENG and ACVRL1 genes, and only 1–3% of case variants occur in SMAD4. HHT clinical manifestations include telangiectasias, epistaxis, and arteriovenous malformations in multiple organ systems. Clinical diagnosis is based on Curaçao Criteria. Here, we describe a pauci-symptomatic 10-year-old girl with an orbital and sinus infectious disease. Her clinical history was unremarkable, except for sporadic, self-limiting epistaxis episodes. She showed finger clubbing and low oxygen saturation levels on pulse oximetry, suggesting a chronic lung disease, and a large lung arteriovenous malformation. She also developed acute neurological symptoms, with evidence of multiple cerebral abscess lesions on MRI. HHT was therefore suspected and confirmed by genetic analysis, which revealed a de novo pathogenic variant in the ENG gene [c.1183G>T p.(Glu395Ter)] found in only 15% of the reads from NGS analysis, performed on peripheral blood lymphocytes, indicating a possible mutational mosaicism. This case outlines that HHT could present with unusual clinical symptoms highlighting the importance of diagnosis using both clinical criteria and genetic test. Full article

With the advancement of new power systems, phase-change materials (PCMs), owing to their ability to convert and store electrical energy, are increasingly recognized as a key solution to the intermittency of power supply. Nevertheless, such materials face challenges, including leakage and low thermal conductivity, which lead to reduced utilization efficiency. In this study, guar gum was used as the macroscopic framework, while self-prepared and optimized silica aerogel microsheets served as the microscopic framework to synergistically encapsulate the polyethylene glycol (PEG). Titanium dioxide (TiO₂) nanoparticles were incorporated to improve overall thermal conductivity, resulting in the composite PCM, GTS-PEG. In-depth characterization demonstrated effective PEG retention within the matrix, with a melting heat storage density of 164.16 J/g. Upon 30 min of continuous heating at 90 °C, the mass loss remained as low as 4.83%, indicating excellent thermal stability. The addition of TiO₂ increased thermal conductivity to 0.53 W/(m·K), representing a 140% boost over unmodified material. As a result, GTS-PEG not only successfully overcomes the leakage and thermal conductivity limitations of conventional PCMs but also, as a green and low-carbon innovative solution, paves a new path for the coordinated optimization and efficient conversion of power grid energy systems. Full article

Background: Anthocyanins and proanthocyanidins (PAs), as flavonoid compounds with potent antioxidant activity, exhibit significant health-promoting and medicinal properties. Black wolfberry (Lycium ruthenicum Murr.) is renowned for its exceptional anthocyanin content; however, the regulatory mechanisms of anthocyanin biosynthesis remain poorly understood, limiting its biotechnological potential. This study aimed to elucidate the transcriptional regulatory function of LrMYB30 in anthocyanin biosynthesis in black wolfberry. Methods: The regulatory function of LrMYB30 was investigated using virus-induced gene silencing (VIGS), yeast one-hybrid assays, and dual-luciferase reporter assays in black wolfberry. Results: VIGS demonstrated that silencing LrMYB30 promoted anthocyanin accumulation while reducing PA content, establishing that the LrMYB30 transcription factor as a negative regulator of anthocyanin synthesis. Yeast one-hybrid and dual-luciferase reporter assays confirmed that LrMYB30 directly binds to and activates the promoter of LrANR, a key structural gene in PA biosynthesis. In contrast, LrMYB30 neither binds to nor suppresses the promoters of the critical anthocyanin biosynthesis genes LrUF3GT and LrDFR. Conclusions: Thus, LrMYB30 redirects the flavonoid metabolic flux from anthocyanin to PA synthesis through transcriptional activation of LrANR during later fruit development, reducing anthocyanin accumulation and delaying coloration. These findings reveal a novel regulatory mechanism in black wolfberry pigmentation and maturation, providing genetic targets for molecular breeding of high-anthocyanin cultivars. Full article

Lung adenocarcinoma (LUAD), the most prevalent subtype of non-small cell lung carcinoma (NSCLC), commonly metastasizes to the brain, particularly in advanced stages. Since brain metastases (BMs) are a leading cause of morbidity and mortality in LUAD patients, their early detection is critical, necessitating the identification of reliable biomarkers. Gangliosides (GGs), a class of bioactive glycosphingolipids involved in cell signaling, adhesion, and immune regulation, have emerged as promising candidates for diagnostic and therapeutic targeting in LUAD-associated brain metastases (BMLA). In this context, ion mobility spectrometry mass spectrometry (IMS-MS) was employed here to analyze GG alterations in BMLA tissues compared to healthy cerebellar control. The results revealed marked differences, including a reduction in the total number of species, altered sialylation profiles, and variations in fatty acid chain length and sphingoid base hydroxylation. GM3, a monosialodihexosylganglioside, was significantly overexpressed in BMLA, supporting its role in tumor progression via immune evasion and oncogenic signaling. Elevated levels of the brain-specific GT1 ganglioside further point to its possible role as a metastasis-associated biomarker, while the presence of asialogangliosides, absent in normal brain, suggests adaptation to the brain microenvironment. Structural modifications such as O-acetylation, fucosylation, and CH₃COO⁻ were more frequent in BMLA, being associated with aggressive tumor phenotypes. Ceramide profiles revealed increased levels of proliferative C16- and C24-ceramides and decreased pro-apoptotic C18-ceramide. Additionally, GM3(d18:1/22:0) and GD3(d18:1/16:0), identified as potential BMLA biomarkers, were structurally characterized using (−) nanoelectrospray ionization (nanoESI) IMS collision-induced dissociation tandem MS (CID MS/MS). Collectively, these findings highlight the clinical potential of GGs for early diagnosis and targeted therapy in BMLA. Full article

Driven by the shipping industry’s pressing need to reduce its environmental impact, methanol has emerged as a promising marine fuel. Methanol-diesel dual-fuel (DF) engines present a viable solution, yet their optimization is challenging due to complex, nonlinear interactions among operational parameters. This study develops an integrated simulation and data-driven framework for multi-objective optimization of a large-bore two-stroke marine DF engine. We first establish a high-fidelity 1D model in GT-POWER, rigorously validated against experimental data with prediction errors within 10% for emissions (NOx, CO, CO₂) and 3% for performance indicators. To address computational constraints, we implement a Polynomial Regression (PR) surrogate model that accurately captures engine response characteristics. The innovative Triple-Adaptive Chaotic Sparrow Search Algorithm (TAC-SSA) serves as the core optimization tool, efficiently exploring the parameter space to generate Pareto-optimal solutions that simultaneously minimize fuel consumption and emissions. The Entropy-Weighted TOPSIS (E-TOPSIS) method then identifies the optimal compromise solution from the Pareto set. At 75% load, the framework determines an optimal configuration: methanol substitution ratio (MSR) = 93.4%; crank angle at the beginning of combustion (CAB) = 2.15 °CA; scavenge air pressure = 1.70 bar; scavenge air temperature = 26.9 °C, achieving concurrent reductions of 7.1% in NOx, 13.3% in CO, 6.1% in CO₂, and 4.1% in specific fuel oil consumption (SFOC) relative to baseline operation. Full article

Background: Immune-regulatory genes such as CTLA-4, FOXO-3, and PTPN-22 influence immune tolerance and metabolic adaptation, but their interaction with environmental factors in type 1 diabetes (T1DM) remains unclear. Methods: In this observational associated study, we analyzed CTLA-4 (rs3087243, rs231775), FOXO-3 (rs2802292, rs9400239), and PTPN-22 (rs12730735) polymorphisms in 277 adults with T1DM, assessing associations with probiotic and vitamin D use, self-reported dietary patterns, metabolic control, autoimmune thyroid disease (AITD), and metabolic dysfunction-associated steatotic liver disease (MASLD). Results: Across the cohort, CTLA-4 rs3087243 G and FOXO-3 rs2802292 T alleles were associated with higher AITD risk (p = 0.016–0.03), significant in both dominant and additive models. The effect persisted by sex: CTLA-4 in women and FOXO-3 in men. Stratified analyses revealed metabolic advantages for CTLA-4 G and FOXO-3 T carriers (vegetarian diet, lower HbA1c, stress adaptation). FOXO-3 rs9400239 T was linked to MASLD (p ≈ 0.037–0.041), with similar trends for CTLA-4 rs231775, stronger in men. Vitamin D supplementation showed protective trends, particularly in FOXO-3 rs2802292 GG and CTLA-4 GG/AG carriers. Conversely, probiotic use was associated with higher AITD in FOXO-3 rs2802292 GT and CTLA-4 rs3087243 GG genotypes. Conclusions: CTLA-4, FOXO-3, and PTPN-22 variants may modulate the metabolic and autoimmune response to environmental factors including nutrients in T1DM. Full article

Using hydrogen in compression-ignition internal combustion engines can reduce pollutant emissions and improve performance by enabling faster and more complete combustion. However, it is essential to determine the optimal injection timing and duration for both hydrogen and conventional fuels. These factors are critical in engine modeling analysis. This study aimed to analyze pollutant emissions, combustion, and engine performance with oxyhydrogen fumigation applied to an instrumented Ricardo E6 engine running on diesel fuel. This analysis, necessary for developing a new predictive combustion model, was calibrated with experimental data in the Gamma Technologies Suite (GTS) simulator. The results show four main effects when increasing the oxyhydrogen flow rate from 0 to 2.8 L per minute (LPM), at an indicated mean effective pressure (IMEP) of 5.3 bar and a speed of 1500 RPM: (I) NO_x levels increased by up to 6%, (II) CO₂ levels decreased by 8%, (III) combustion durations remained relatively stable, and (IV) brake specific fuel consumption decreased by 8%. Overall, adding hydrogen to the intake flow of the compression-ignition engine reduced CO₂ emissions and enhanced indicated thermal efficiency. Full article

This study systematically investigates the post-construction settlement behavior of high-fill red clay embankments, focusing on the influences of three key factors (water content, degree of compaction, and lift thickness) and the effectiveness of geogrid-based reinforcement measures. A three-dimensional finite-element model based on the Mohr–Coulomb constitutive theory was established using MIDAS GTS NX 2022 R1 to simulate staged construction processes and long-term settlement under self-weight loading. The results indicate that settlement is predominantly concentrated in the upper fill zone adjacent to the slope surface, with displacement contours sagging inward toward the fill interior, while the underlying foundation undergoes negligible deformation. An elevated water content and reduced degree of compaction significantly enhance the compressibility of red clay, leading to increased settlement magnitudes and prolonged stabilization periods. Excessively thick lifts result in inadequate deep compaction, thereby inducing larger final settlements. Two reinforcement schemes (geogrid combined with anti-slide piles and geogrid combined with a gravity retaining wall) were verified to effectively mitigate post-construction settlement, with the former achieving a more pronounced improvement in the embankment stability coefficient. Based on the comprehensive analysis, optimal construction control parameters for high-fill red clay embankments are proposed: precise regulation of water content, maximization of compaction degree, and adoption of a lift thickness of approximately 30 cm. The findings of this study provide quantitative technical support and design references for the settlement control of similar high-fill red clay embankment projects in southern China’s mountainous and hilly regions. Full article

The solid oxide fuel cell–gas turbine (SOFC-GT) hybrid system is confronted with challenges related to system integration and coordinated control. In this study, a Controller Hardware-in-the-Loop Simulation (C-HILS) platform is constructed to validate its digital solutions. The C-HILS platform integrates the Advanced Process Simulation System (APROS), LabVIEW 2020 programming software, NI PXI hardware, and a distributed control system (DCS). Specifically, bidirectional data transmission between the simulation software and the DCS is facilitated through LabVIEW and PXI, leveraging the OLE for Process Control (OPC) protocol and physical Input and Output (I/O) channels. The dynamic SOFC-GT model developed in APROS demonstrates good consistency with design values, with relative errors below 4%. The DCS configuration employs PID controllers to achieve control over total power, SOFC fuel utilization, and gas turbine rotational speed. Experiments under transient conditions reveal that, despite discrepancies in dynamic responses between C-HILS and full-digital simulations, both can achieve stable control. This C-HILS platform effectively integrates virtual models with physical hardware, offering a reliable environment for verifying SOFC-GT control strategies and digital solutions, and thus facilitating the digital transformation of energy systems. Full article

Gully is the most visible sign of land degradation, but its effects on runoff and sediment dynamics during snowmelt conditions remain poorly understood. This study monitored a typical gully in the Mollisols region of Northeast China to investigate runoff and sediment transport at the Gully Head (GH) and Gully Tail (GT) during spring snowmelt. Results showed that gully significantly influenced snow distribution, with deeper snow accumulation than on slopes. Runoff at the GH lasted 9 days, while gully connectivity extended catchment runoff by 10 additional days. Runoff temporal variation at GH and GT was broadly consistent, with GH contributing 7.4% of the total runoff at GT. Peak runoff discharge and sediment concentration occurred on the sixth day after snowmelt onset, driven by snow cover and air temperature. Gully significantly increased the sediment concentration from the upslope runoff. Runoff responses to temperature varied by melt stage, with GT showing higher sensitivity, especially under high-runoff conditions. High sediment yield was linked not to snow depth, but to late-stage snowmelt and soil thawing, when erosion sensitivity peaked. Hysteresis analysis revealed dominant clockwise loops during this phase, contrasting with figure-eight and counterclockwise patterns in other stages. These findings highlight the importance of targeting erosion control during late snowmelt when runoff intensifies and soils thaw. Full article

The gut microbiota of insects plays a fundamental role in modulating host physiology, including nutrition, development, and adaptability to environmental challenges. The rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), is a major invasive pest of rice worldwide, yet the composition and functional profile of its gut microbial community remain poorly characterized. Here, we employed metagenome sequencing on the Illumina NovaSeq X Plus platform to explore the gut microbial diversity and predicted functions in adults of L. oryzophilus. Our results revealed a rich microbial community, comprising 26 phyla, 42 classes, 72 orders, 111 families, and 191 genera. The bacterial microbiota was overwhelmingly dominated by the phylum Proteobacteria (85.13% of total abundance). At the genus level, Pantoea (48.86%) was the most predominant taxon, followed by Wolbachia (14.57%) and Rickettsia (11.81%). KEGG analysis suggested that the gut microbiota is primarily associated with metabolic pathways such as membrane transport, carbohydrate and amino acid metabolism, cofactor and vitamin metabolism, energy metabolism, and signal transduction. eggNOG annotation further highlighted significant gene representation in amino acid and carbohydrate transport and metabolism, while CAZy annotation revealed glycosyl transferases (GTs) and glycoside hydrolases (GHs) as the dominant carbohydrate-active enzymes. This study provides the first comprehensive insight into the gut microbiome of L. oryzophilus adults, highlighting its potential role in the ecological success of this invasive pest. Our findings lay groundwork for future research aimed at developing novel microbial-based strategies for the sustainable management of L. oryzophilus. Full article

Placer deposits constitute important secondary resources for economically valuable minerals, including rare earth elements (REEs) and heavy minerals such as zircon, rutile, and ilmenite. In this study, representative samples from the Hantepe placer deposit (Çanakkale, Türkiye) were processed to investigate the occurrence, distribution, and beneficiation potential of REE-bearing minerals. The ore was subjected to size classification, followed by gravity concentration on a shaking table and subsequent magnetic separation using a low-intensity disc separator. The resulting products were characterized by X-ray diffraction and X-ray fluorescence. The dominant REE-host minerals were identified as titanite, zircon, apatite, monazite and, allanite, accompanied by magnetite, hematite, quartz, and feldspar as gangue constituents. The non-magnetic final concentrate achieved substantial upgrading of critical elements, with Ce increasing from 868 g/t to 5716 g/t, Nd from 308 g/t to 2308 g/t, and Zr from 1435 g/t to 9748 g/t. Additionally, the magnetic concentrate (7.0 wt.%) was strongly enriched in Fe₂O₃ (70.26%) and V (2359 g/t), indicating its potential suitability as an Fe–V source. Overall, the results demonstrate that combined gravity and magnetic separation constitutes an effective beneficiation strategy for critical mineral recovery from placer systems. These findings establish a strong basis for future pilot-scale studies and the techno-economic evaluation of the Hantepe deposit as an emerging source of strategic and industrially relevant heavy minerals. Full article