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Search Results (8,145)

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Keywords = Essential oils

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42 pages, 5770 KiB  
Review
Echoes from Below: A Systematic Review of Cement Bond Log Innovations Through Global Patent Analysis
by Lim Shing Wang, Muhammad Haarith Firdaous and Pg Emeroylariffion Abas
Inventions 2025, 10(4), 67; https://doi.org/10.3390/inventions10040067 (registering DOI) - 2 Aug 2025
Abstract
Maintaining well integrity is essential in the oil and gas industry to prevent environmental hazards, operational risks, and economic losses. Cement bond log (CBL) tools are essential in evaluating cement bonding and ensuring wellbore stability. This study presents a patent landscape review of [...] Read more.
Maintaining well integrity is essential in the oil and gas industry to prevent environmental hazards, operational risks, and economic losses. Cement bond log (CBL) tools are essential in evaluating cement bonding and ensuring wellbore stability. This study presents a patent landscape review of CBL technologies, based on 3473 patent documents from the Lens.org database. After eliminating duplicates and irrelevant entries, 167 granted patents were selected for in-depth analysis. These were categorized by technology type (wave, electrical, radiation, neutron, and other tools) and by material focus (formation, casing, cement, and borehole fluid). The findings reveal a dominant focus on formation evaluation (59.9%) and a growing reliance on wave-based (22.2%) and other advanced tools (25.1%), indicating a shift toward high-precision diagnostics. Geographically, 75% of granted patents were filed through the U.S. Patent and Trademark Office, and 97.6% were held by companies, underscoring the dominance of corporate innovation and the minimal presence of academia and individuals. The review also identifies notable patents that reflect significant technical innovations and discusses their role in advancing diagnostic capabilities. These insights emphasize the need for broader collaboration and targeted research to advance well integrity technologies in line with industry goals for operational performance and safety. Full article
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38 pages, 6505 KiB  
Review
Trends in Oil Spill Modeling: A Review of the Literature
by Rodrigo N. Vasconcelos, André T. Cunha Lima, Carlos A. D. Lentini, José Garcia V. Miranda, Luís F. F. de Mendonça, Diego P. Costa, Soltan G. Duverger and Elaine C. B. Cambui
Water 2025, 17(15), 2300; https://doi.org/10.3390/w17152300 (registering DOI) - 2 Aug 2025
Abstract
Oil spill simulation models are essential for predicting the oil spill behavior and movement in marine environments. In this study, we comprehensively reviewed a large and diverse body of peer-reviewed literature obtained from Scopus and Web of Science. Our initial analysis phase focused [...] Read more.
Oil spill simulation models are essential for predicting the oil spill behavior and movement in marine environments. In this study, we comprehensively reviewed a large and diverse body of peer-reviewed literature obtained from Scopus and Web of Science. Our initial analysis phase focused on examining trends in scientific publications, utilizing the complete dataset derived after systematic screening and database integration. In the second phase, we applied elements of a systematic review to identify and evaluate the most influential contributions in the scientific field of oil spill simulations. Our analysis revealed a steady and accelerating growth of research activity over the past five decades, with a particularly notable expansion in the last two. The field has also experienced a marked increase in collaborative practices, including a rise in international co-authorship and multi-authored contributions, reflecting a more global and interdisciplinary research landscape. We cataloged the key modeling frameworks that have shaped the field from established systems such as OSCAR, OIL-MAP/SIMAP, and GNOME to emerging hybrid and Lagrangian approaches. Hydrodynamic models were consistently central, often integrated with biogeochemical, wave, atmospheric, and oil-spill-specific modules. Environmental variables such as wind, ocean currents, and temperature were frequently used to drive model behavior. Geographically, research has concentrated on ecologically and economically sensitive coastal and marine regions. We conclude that future progress will rely on the real-time integration of high-resolution environmental data streams, the development of machine-learning-based surrogate models to accelerate computations, and the incorporation of advanced biodegradation and weathering mechanisms supported by experimental data. These advancements are expected to enhance the accuracy, responsiveness, and operational value of oil spill modeling tools, supporting environmental monitoring and emergency response. Full article
(This article belongs to the Special Issue Advanced Remote Sensing for Coastal System Monitoring and Management)
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15 pages, 2791 KiB  
Article
In Vitro and In Vivo Efficacy of the Essential Oil from the Leaves of Annona amazonica R.E. Fries (Annonaceae) Against Liver Cancer
by Maria V. L. de Castro, Milena C. F. de Lima, Gabriela A. da C. Barbosa, Sabrine G. Carvalho, Amanda M. R. M. Coelho, Luciano de S. Santos, Valdenizia R. Silva, Rosane B. Dias, Milena B. P. Soares, Emmanoel V. Costa and Daniel P. Bezerra
Molecules 2025, 30(15), 3248; https://doi.org/10.3390/molecules30153248 (registering DOI) - 2 Aug 2025
Abstract
Annona amazonica R.E. Fries (synonyms Annona amazonica var. lancifolia R.E. Fries), popularly known in Brazil as “envireira”, is a tropical tree belonging to the Annonaceae family and is traditionally used as a food source. In this work, the in vitro and in vivo [...] Read more.
Annona amazonica R.E. Fries (synonyms Annona amazonica var. lancifolia R.E. Fries), popularly known in Brazil as “envireira”, is a tropical tree belonging to the Annonaceae family and is traditionally used as a food source. In this work, the in vitro and in vivo anti-liver cancer effects of essential oil (EO) from A. amazonica leaves were investigated for the first time. The chemical composition of the EO was evaluated via GC–MS and GC–FID. The alamar blue assay was used to evaluate the cytotoxicity of EOs against different cancerous and noncancerous cell lines. Cell cycle analyses, YO-PRO-1/PI staining, and rhodamine 123 staining were performed via flow cytometry in HepG2 cells treated with EO. The in vivo antitumor activity of EO was evaluated in NSG mice that were xenografted with HepG2 cells and treated with EO at a dose of 60 mg/kg. The major constituents (>5%) of the EO were (E)-caryophyllene (32.01%), 1,8-cineole (13.93%), α-copaene (7.77%), α-humulene (7.15%), and α-pinene (5.13%). EO increased apoptosis and proportionally decreased the number of viable HepG2 cells. The induction of DNA fragmentation and cell shrinkage together with a significant reduction in the ΔΨm in EO-treated HepG2 cells confirmed that EO can induce apoptosis. A significant 39.2% inhibition of tumor growth in vivo was detected in EO-treated animals. These data indicate the anti-liver cancer potential of EO from A. amazonica leaves. Full article
(This article belongs to the Special Issue Advances and Opportunities of Natural Products in Drug Discovery)
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46 pages, 2160 KiB  
Review
Potential of Plant-Based Oil Processing Wastes/By-Products as an Alternative Source of Bioactive Compounds in the Food Industry
by Elifsu Nemli, Deniz Günal-Köroğlu, Resat Apak and Esra Capanoglu
Foods 2025, 14(15), 2718; https://doi.org/10.3390/foods14152718 (registering DOI) - 2 Aug 2025
Abstract
The plant-based oil industry contributes significantly to food waste/by-products in the form of underutilized biomass, including oil pomace, cake/meal, seeds, peels, wastewater, etc. These waste/by-products contain a significant quantity of nutritious and bioactive compounds (phenolics, lignans, flavonoids, dietary fiber, proteins, and essential minerals) [...] Read more.
The plant-based oil industry contributes significantly to food waste/by-products in the form of underutilized biomass, including oil pomace, cake/meal, seeds, peels, wastewater, etc. These waste/by-products contain a significant quantity of nutritious and bioactive compounds (phenolics, lignans, flavonoids, dietary fiber, proteins, and essential minerals) with proven health-promoting effects. The utilization of them as natural, cost-effective, and food-grade functional ingredients in novel food formulations holds considerable potential. This review highlights the potential of waste/by-products generated during plant-based oil processing as a promising source of bioactive compounds and covers systematic research, including recent studies focusing on innovative extraction and processing techniques. It also sheds light on their promising potential for valorization as food ingredients, with a focus on specific examples of food fortification. Furthermore, the potential for value creation in the food industry is emphasized, taking into account associated challenges and limitations, as well as future perspectives. Overall, the current information suggests that the valorization of plant-based oil industry waste and by-products for use in the food industry could substantially reduce malnutrition and poverty, generate favorable health outcomes, mitigate environmental concerns, and enhance economic profit in a sustainable way by developing health-promoting, environmentally sustainable food systems. Full article
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11 pages, 459 KiB  
Case Report
Urinary Multidrug-Resistant Klebsiella pneumoniae: Essential Oil Countermeasures in a One Health Case Report
by Mălina-Lorena Mihu, Cristiana Ştefania Novac, Smaranda Crăciun, Nicodim Iosif Fiţ, Cosmina Maria Bouari, George Cosmin Nadăş and Sorin Răpuntean
Microorganisms 2025, 13(8), 1807; https://doi.org/10.3390/microorganisms13081807 (registering DOI) - 1 Aug 2025
Abstract
Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is eroding therapeutic options for urinary tract infections. We isolated a multidrug-resistant strain from the urine of a chronically bacteriuric patient and confirmed its identity by Vitek-2 and MALDI-TOF MS. Initial disk-diffusion profiling against 48 antibiotics revealed susceptibility to [...] Read more.
Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is eroding therapeutic options for urinary tract infections. We isolated a multidrug-resistant strain from the urine of a chronically bacteriuric patient and confirmed its identity by Vitek-2 and MALDI-TOF MS. Initial disk-diffusion profiling against 48 antibiotics revealed susceptibility to only 5 agents. One month later, repeat testing showed that tetracycline alone remained active, highlighting the strain’s rapidly evolving resistome. Given the scarcity of drug options, we performed an “aromatogram” with seven pure essential oils, propolis, and two commercial phytotherapeutic blends. Biomicin Forte® produced a 30 mm bactericidal halo, while thyme, tea tree, laurel, and palmarosa oils yielded clear inhibition zones of 11–22 mm. These in vitro data demonstrate that carefully selected plant-derived products can target CR-Kp where conventional antibiotics fail. Integrating aromatogram results into One Health’s stewardship plans may therefore help preserve last-line antibiotics and provide adjunctive options for persistent urinary infections. Full article
(This article belongs to the Special Issue Infectious Disease Surveillance in Romania)
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20 pages, 10391 KiB  
Article
Sustainable Substitution of Petroleum-Based Processing Oils with Soybean-Derived Alternatives in Styrene–Butadiene Rubber: Effects on Processing Behavior and Mechanical Properties
by Yang-Wei Lin, Tsung-Yi Chen, Chen-Yu Chueh, Yi-Ting Chen, Tsunghsueh Wu and Hsi-Ming Hsieh
Polymers 2025, 17(15), 2129; https://doi.org/10.3390/polym17152129 (registering DOI) - 1 Aug 2025
Abstract
This study evaluates the replacement of petroleum-based naphthenic oil with four types of soybean-derived alternatives—virgin soybean oil (SBO), epoxidized SBO (ESBO), expired SBO, and recycled SBO—in styrene–butadiene rubber (SBR) composites. The materials were tested in both staining rubber (SR) and non-staining rubber (NSR) [...] Read more.
This study evaluates the replacement of petroleum-based naphthenic oil with four types of soybean-derived alternatives—virgin soybean oil (SBO), epoxidized SBO (ESBO), expired SBO, and recycled SBO—in styrene–butadiene rubber (SBR) composites. The materials were tested in both staining rubber (SR) and non-staining rubber (NSR) systems to assess processing characteristics, mechanical performance, and environmental durability. Among the alternatives, SBO demonstrated the best overall performance, improving processability and tensile strength by over 10%, while ESBO enhanced ozone resistance by 35% due to its epoxide functionality. Expired and recycled SBOs maintained essential mechanical properties within 90% of virgin SBO values. The full replacement of CH450 with SBO in tire prototypes resulted in burst strength exceeding 1000 kPa and stable appearance after 5000 km of road testing. To validate industrial relevance, the developed green tire was exhibited at the 2025 Taipei International Cycle Show, attracting interest from international buyers and stakeholders for its eco-friendly composition and carbon footprint reduction potential, thereby demonstrating both technical feasibility and commercial viability. Full article
(This article belongs to the Special Issue Functional Polymers and Their Composites for Sustainable Development)
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38 pages, 959 KiB  
Review
Emerging Trends in Active Packaging for Food: A Six-Year Review
by Mariana A. Andrade, Cássia H. Barbosa, Regiane Ribeiro-Santos, Sidney Tomé, Ana Luísa Fernando, Ana Sanches Silva and Fernanda Vilarinho
Foods 2025, 14(15), 2713; https://doi.org/10.3390/foods14152713 (registering DOI) - 1 Aug 2025
Viewed by 42
Abstract
The development of active food packaging has evolved rapidly in recent years, offering innovative solutions to enhance food preservation and safety while addressing sustainability challenges. This review compiles and analyzes recent advancements (2019–2024) in release-type active packaging, focusing on essential oils, natural extracts, [...] Read more.
The development of active food packaging has evolved rapidly in recent years, offering innovative solutions to enhance food preservation and safety while addressing sustainability challenges. This review compiles and analyzes recent advancements (2019–2024) in release-type active packaging, focusing on essential oils, natural extracts, and phenolic compounds as active agents. Primarily plant-derived, these compounds exhibit significant antioxidant and antimicrobial activities, extending shelf life and enhancing food quality. Technological strategies such as encapsulation and polymer blending have been increasingly adopted to overcome challenges related to volatility, solubility, and sensory impact. Integrating bio-based polymers, including chitosan, starch, and polylactic acid, further supports the development of environmentally friendly packaging systems. This review also highlights trends in compound-specific research, release mechanisms, and commercial applications, including a detailed analysis of patents and case studies across various food matrices. These developments have already been translated into practical applications, such as antimicrobial sachets for meat and essential oil-based pads for fresh produce. Moreover, by promoting the valorization of agro-industrial by-products and the use of biodegradable materials, emission-type active packaging contributes to the principles of the circular economy. This comprehensive overview underscores the potential of natural bioactive compounds in advancing sustainable and functional food packaging technologies. Full article
20 pages, 1334 KiB  
Article
Chitosan Nanoparticles Encapsulating Oregano Oil: Effects on In Vitro Ruminal Fermentation from Goat Rumen Fluid
by Gerardo Méndez-Zamora, Jorge R. Kawas, Sara Paola Hernández-Martínez, Gustavo Sobrevilla-Hernández, Sugey Ramona Sinagawa-García, Daniela S. Rico-Costilla and Jocelyn Cyan López-Puga
Animals 2025, 15(15), 2261; https://doi.org/10.3390/ani15152261 (registering DOI) - 1 Aug 2025
Viewed by 47
Abstract
This study evaluated the effects of liquid oregano oil, chitosan nanoparticles with encapsulated liquid oregano oil, and a negative control of empty chitosan nanoparticles on in vitro ruminal fermentation. Three Boer goats were used as ruminal fluid donors, fed with a formulated ration [...] Read more.
This study evaluated the effects of liquid oregano oil, chitosan nanoparticles with encapsulated liquid oregano oil, and a negative control of empty chitosan nanoparticles on in vitro ruminal fermentation. Three Boer goats were used as ruminal fluid donors, fed with a formulated ration for 21 d for inoculum adaptation. Treatments tested on in vitro assays were diet without oregano oil or nanoparticles (CON); diet with 100 ppm of oregano oil in nanoparticles (100N); diet with 300 ppm of liquid oregano oil (300L); diet with 300 ppm of oregano oil in nanoparticles (300N); and diet with 300 ppm of empty nanoparticles (300CHN). The variables studied were in vitro dry matter digestibility (ivDMD), in vitro neutral detergent fiber digestibility (ivNDFDom), total gas production (TGP), ammonia nitrogen concentration (NH3), and pH. The experimental design was a randomized complete block design. Linear and quadratic regressions were used to identify dependence and inflection points. The ivDMD increased at 12, 36, and 48 h, with 300N and with 300L exhibiting increased ivNDFDom at 36 h. Ruminal pH was highest (p < 0.05) with 300CHN at 36 h. For first-order regression analysis of TGP, coefficients (β) were highest (p < 0.05) for 300N. In conclusion, 300N increased ruminal fermentation in vitro, as reflected by increases in ivDMD, ivNDFDom, and TGP. Full article
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22 pages, 6617 KiB  
Article
Natural Plant Oils as Anti—Algae Biocides for Sustainable Application in Cultural Heritage Protection
by Michał Komar, Nathnael Derese, Kamil Szymczak, Paulina Nowicka-Krawczyk and Beata Gutarowska
Sustainability 2025, 17(15), 6996; https://doi.org/10.3390/su17156996 (registering DOI) - 1 Aug 2025
Viewed by 64
Abstract
The prevention of biofilm formation and algal biodeterioration on building materials, particularly on cultural heritage sites, is a growing concern. Due to regulatory restrictions on conventional algicidal biocides in Europe, natural alternatives such as essential oils are gaining interest for their potential use [...] Read more.
The prevention of biofilm formation and algal biodeterioration on building materials, particularly on cultural heritage sites, is a growing concern. Due to regulatory restrictions on conventional algicidal biocides in Europe, natural alternatives such as essential oils are gaining interest for their potential use in heritage conservation. This study evaluates the anti-algal activity of Salvia officinalis and Equisetum arvense (essential oils, hydrolates, and extracts) against a mixed culture of five green algae species (Bracteacoccus minor, Stichococcus bacillaris, Klebsormidium nitens, Chloroidium saccharophilum, and Diplosphaera chodatii). The plant materials were processed using hydrodistillation and solvent extraction, followed by chemical characterization through gas chromatography–mass spectrometry (GC-MS). Biological efficacy was assessed by measuring algal growth inhibition, changes in biomass colour, chlorophyll a concentration, and fluorescence. S. officinalis yielded higher extract quantities (extraction yield: 23%) than E. arvense and contained bioactive compounds such as thujone, camphor, and cineole, which correlated with its strong anti-algal effects. The essential oil of S. officinalis demonstrated the highest efficacy, significantly inhibiting biofilm formation (zones of inhibition: 15–94 mm) and photosynthetic activity at 0.5% concentration (reduction in chlorophyll a concentration 90–100%), without causing visible discolouration of treated surfaces (∆E < 2). These findings highlight the potential of S. officinalis essential oil as a natural, effective, and material-safe algicidal biocide for the sustainable protection of cultural heritage sites. Full article
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36 pages, 3621 KiB  
Review
Harnessing Molecular Phylogeny and Chemometrics for Taxonomic Validation of Korean Aromatic Plants: Integrating Genomics with Practical Applications
by Adnan Amin and Seonjoo Park
Plants 2025, 14(15), 2364; https://doi.org/10.3390/plants14152364 - 1 Aug 2025
Viewed by 219
Abstract
Plant genetics and chemotaxonomic analysis are considered key parameters in understanding evolution, plant diversity and adaptation. Korean Peninsula has a unique biogeographical landscape that supports various aromatic plant species, each with considerable ecological, ethnobotanical, and pharmacological significance. This review aims to provide a [...] Read more.
Plant genetics and chemotaxonomic analysis are considered key parameters in understanding evolution, plant diversity and adaptation. Korean Peninsula has a unique biogeographical landscape that supports various aromatic plant species, each with considerable ecological, ethnobotanical, and pharmacological significance. This review aims to provide a comprehensive overview of the chemotaxonomic traits, biological activities, phylogenetic relationships and potential applications of Korean aromatic plants, highlighting their significance in more accurate identification. Chemotaxonomic investigations employing techniques such as gas chromatography mass spectrometry, high-performance liquid chromatography, and nuclear magnetic resonance spectroscopy have enabled the identification of essential oils and specialized metabolites that serve as valuable taxonomic and diagnostic markers. These chemical traits play essential roles in species delimitation and in clarifying interspecific variation. The biological activities of selected taxa are reviewed, with emphasis on antimicrobial, antioxidant, anti-inflammatory, and cytotoxic effects, supported by bioassay-guided fractionation and compound isolation. In parallel, recent advances in phylogenetic reconstruction employing DNA barcoding, internal transcribed spacer regions, and chloroplast genes such as rbcL and matK are examined for their role in clarifying taxonomic uncertainties and inferring evolutionary lineages. Overall, the search period was from year 2001 to 2025 and total of 268 records were included in the study. By integrating phytochemical profiling, pharmacological evidence, and molecular systematics, this review highlights the multifaceted significance of Korean endemic aromatic plants. The conclusion highlights the importance of multidisciplinary approaches including metabolomics and phylogenomics in advancing our understanding of species diversity, evolutionary adaptation, and potential applications. Future research directions are proposed to support conservation efforts. Full article
(This article belongs to the Special Issue Applications of Bioinformatics in Plant Science)
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48 pages, 2506 KiB  
Article
Enhancing Ship Propulsion Efficiency Predictions with Integrated Physics and Machine Learning
by Hamid Reza Soltani Motlagh, Seyed Behbood Issa-Zadeh, Md Redzuan Zoolfakar and Claudia Lizette Garay-Rondero
J. Mar. Sci. Eng. 2025, 13(8), 1487; https://doi.org/10.3390/jmse13081487 - 31 Jul 2025
Viewed by 165
Abstract
This research develops a dual physics-based machine learning system to forecast fuel consumption and CO2 emissions for a 100 m oil tanker across six operational scenarios: Original, Paint, Advanced Propeller, Fin, Bulbous Bow, and Combined. The combination of hydrodynamic calculations with Monte [...] Read more.
This research develops a dual physics-based machine learning system to forecast fuel consumption and CO2 emissions for a 100 m oil tanker across six operational scenarios: Original, Paint, Advanced Propeller, Fin, Bulbous Bow, and Combined. The combination of hydrodynamic calculations with Monte Carlo simulations provides a solid foundation for training machine learning models, particularly in cases where dataset restrictions are present. The XGBoost model demonstrated superior performance compared to Support Vector Regression, Gaussian Process Regression, Random Forest, and Shallow Neural Network models, achieving near-zero prediction errors that closely matched physics-based calculations. The physics-based analysis demonstrated that the Combined scenario, which combines hull coatings with bulbous bow modifications, produced the largest fuel consumption reduction (5.37% at 15 knots), followed by the Advanced Propeller scenario. The results demonstrate that user inputs (e.g., engine power: 870 kW, speed: 12.7 knots) match the Advanced Propeller scenario, followed by Paint, which indicates that advanced propellers or hull coatings would optimize efficiency. The obtained insights help ship operators modify their operational parameters and designers select essential modifications for sustainable operations. The model maintains its strength at low speeds, where fuel consumption is minimal, making it applicable to other oil tankers. The hybrid approach provides a new tool for maritime efficiency analysis, yielding interpretable results that support International Maritime Organization objectives, despite starting with a limited dataset. The model requires additional research to enhance its predictive accuracy using larger datasets and real-time data collection, which will aid in achieving global environmental stewardship. Full article
(This article belongs to the Special Issue Machine Learning for Prediction of Ship Motion)
17 pages, 2562 KiB  
Article
Comparative Stability and Anesthetic Evaluation of Holy Basil Essential Oil Formulated in SNEDDS and Microemulsion Systems in Cyprinus carpio var. Koi
by Kantaporn Kheawfu, Chuda Chittasupho, Surachai Pikulkaew, Wasana Chaisri and Taepin Junmahasathien
Pharmaceutics 2025, 17(8), 997; https://doi.org/10.3390/pharmaceutics17080997 (registering DOI) - 31 Jul 2025
Viewed by 117
Abstract
Background/Objectives: Holy basil (Ocimum tenuiflorum L.) essential oil exhibits antioxidant, antimicrobial, and anesthetic activities, mainly due to eugenol, methyl eugenol, and β-caryophyllene. However, its clinical application is limited by poor water solubility, instability, and low bioavailability. This study developed and compared two [...] Read more.
Background/Objectives: Holy basil (Ocimum tenuiflorum L.) essential oil exhibits antioxidant, antimicrobial, and anesthetic activities, mainly due to eugenol, methyl eugenol, and β-caryophyllene. However, its clinical application is limited by poor water solubility, instability, and low bioavailability. This study developed and compared two delivery systems, self-nanoemulsifying drug delivery systems (SNEDDS) and microemulsions (ME), to enhance their stability and fish anesthetic efficacy. Methods: The optimized SNEDDS (25% basil oil, 8.33% coconut oil, 54.76% Tween 80, 11.91% PEG 400) and ME (12% basil oil, 32% Tween 80, 4% sorbitol, 12% ethanol, 40% water) were characterized for droplet size, PDI, zeta potential, pH, and viscosity. Stability was evaluated by monitoring droplet size and PDI over time and by determining the retention of eugenol, methyl eugenol, and β-caryophyllene after storage at 45 °C. Fish anesthetic efficacy was tested in koi carp (Cyprinus carpio var. koi). Results: SNEDDS maintained a small droplet size (~22.78 ± 1.99 nm) and low PDI (0.188 ± 0.088 at day 60), while ME showed significant size enlargement (up to 177.10 ± 47.50 nm) and high PDI (>0.5). After 90 days at 45 °C, SNEDDS retained 94.45% eugenol, 94.08% methyl eugenol, and 88.55% β-caryophyllene, while ME preserved 104.76%, 103.53%, and 94.47%, respectively. In vivo testing showed that SNEDDS achieved faster anesthesia (114.70 ± 24.80 s at 120 ppm) and shorter recovery (379.60 ± 15.61 s) than ME (134.90 ± 4.70 s; 473.80 ± 16.94 s). Ethanol failed to induce anesthesia at 40 ppm and performed poorly compared to SNEDDS and ME at other concentrations (p < 0.0001). Conclusions: SNEDDS demonstrated superior physical stability and fish anesthetic performance compared to ME. These findings support SNEDDS as a promising formulation for delivering holy basil essential oil in biomedical and aquaculture applications. Full article
(This article belongs to the Special Issue Applications of Nanotechnology in Veterinary Drug Delivery)
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16 pages, 938 KiB  
Review
Enhancing Oil Content in Oilseed Crops: Genetic Insights, Molecular Mechanisms, and Breeding Approaches
by Guizhen Gao, Lu Zhang, Panpan Tong, Guixin Yan and Xiaoming Wu
Int. J. Mol. Sci. 2025, 26(15), 7390; https://doi.org/10.3390/ijms26157390 (registering DOI) - 31 Jul 2025
Viewed by 230
Abstract
Vegetable oils are essential for human nutrition and industrial applications. With growing global demand, increasing oil content in oilseed crops has become a top priority. This review synthesizes recent progress in understanding the genetic, environmental, and molecular mechanisms regulating oil content, and presents [...] Read more.
Vegetable oils are essential for human nutrition and industrial applications. With growing global demand, increasing oil content in oilseed crops has become a top priority. This review synthesizes recent progress in understanding the genetic, environmental, and molecular mechanisms regulating oil content, and presents biotechnological strategies to enhance oil accumulation in major oilseed crops. Oil biosynthesis is governed by intricate genetic–environmental interactions. Environmental factors and agronomic practices significantly impact oil accumulation dynamics. Quantitative trait loci (QTL) mapping and genome-wide association studies (GWAS) have identified key loci and candidate genes involved in lipid biosynthesis pathways. Transcription factors and epigenetic regulators further fine-tune oil accumulation. Biotechnological approaches, including marker-assisted selection (MAS) and CRISPR/Cas9-mediated genome editing, have successfully generated high-oil-content variants. Future research should integrate multi-omics data, leverage AI-based predictive breeding, and apply precision genome editing to optimize oil yield while maintaining seed quality. This review provides critical references for the genetic improvement and breeding of high- and ultra-high-oil-content varieties in oilseed crops. Full article
(This article belongs to the Special Issue Rapeseed: Genetic Breeding, Key Trait Mining and Genome)
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25 pages, 2023 KiB  
Article
Geographical Origin Authentication of Leaves and Drupes from Olea europaea via 1H NMR and Excitation–Emission Fluorescence Spectroscopy: A Data Fusion Approach
by Duccio Tatini, Flavia Bisozzi, Sara Costantini, Giacomo Fattori, Amedeo Boldrini, Michele Baglioni, Claudia Bonechi, Alessandro Donati, Cristiana Tozzi, Angelo Riccaboni, Gabriella Tamasi and Claudio Rossi
Molecules 2025, 30(15), 3208; https://doi.org/10.3390/molecules30153208 - 30 Jul 2025
Viewed by 164
Abstract
Geographical origin authentication of agrifood products is essential for ensuring their quality, preventing fraud, and maintaining consumers’ trust. In this study, we used proton nuclear magnetic resonance (1H NMR) and excitation–emission matrix (EEM) fluorescence spectroscopy combined with chemometric methods for the [...] Read more.
Geographical origin authentication of agrifood products is essential for ensuring their quality, preventing fraud, and maintaining consumers’ trust. In this study, we used proton nuclear magnetic resonance (1H NMR) and excitation–emission matrix (EEM) fluorescence spectroscopy combined with chemometric methods for the geographical origin characterization of olive drupes and leaves from different Tuscany subregions, where olive oil production is relevant. Single-block approaches were implemented for individual datasets, using principal component analysis (PCA) for data visualization and Soft Independent Modeling of Class Analogy (SIMCA) for sample classification. 1H NMR spectroscopy provided detailed metabolomic profiles, identifying key compounds such as polyphenols and organic acids that contribute to geographical differentiation. EEM fluorescence spectroscopy, in combination with Parallel Factor Analysis (PARAFAC), revealed distinctive fluorescence signatures associated with polyphenolic content. A mid-level data fusion strategy, integrating the common dimensions (ComDim) method, was explored to improve the models’ performance. The results demonstrated that both spectroscopic techniques independently provided valuable insights in terms of geographical characterization, while data fusion further improved the model performances, particularly for olive drupes. Notably, this study represents the first attempt to apply EEM fluorescence for the geographical classification of olive drupes and leaves, highlighting its potential as a complementary tool in geographic origin authentication. The integration of advanced spectroscopic and chemometric methods offers a reliable approach for the differentiation of samples from closely related areas at a subregional level. Full article
(This article belongs to the Section Food Chemistry)
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14 pages, 923 KiB  
Article
Mitigating Effects of Rosmarinus officinalis Essential Oil and Sugar Beet Pulp on Immune Response and Growth Performance of Heat-Stressed Lambs
by Maria Giovanna Ciliberti, Rosaria Marino, Mariangela Caroprese, Cristina Stango, Agostino Sevi and Marzia Albenzio
Animals 2025, 15(15), 2241; https://doi.org/10.3390/ani15152241 - 30 Jul 2025
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Abstract
Dietary supplementation under high ambient temperatures can be considered crucial for supporting immune responses in livestock. In the present paper, Rosmarinus officinalis essential oil (REO) alone and in combination with dried sugar beet pulp (REO + B) was included in the diet of [...] Read more.
Dietary supplementation under high ambient temperatures can be considered crucial for supporting immune responses in livestock. In the present paper, Rosmarinus officinalis essential oil (REO) alone and in combination with dried sugar beet pulp (REO + B) was included in the diet of lambs during the fattening period under heat stress conditions. Environmental conditions and physiological and growth parameters of lambs were monitored throughout the trial. Plasma samples were collected to evaluate cytokine secretion (IL-1β, IL-6, and IL-10). Notably, REO inclusion reduced rectal temperature and respiration rate while increasing pro-inflammatory cytokines (IL-1β and IL-6) at the same time, suggesting an enhanced immune response without compromising growth performance. Full article
(This article belongs to the Section Animal Physiology)
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