Search Results (112)

Climate change and population growth are intensifying global food security challenges by reducing agricultural productivity and increasing reliance on fertilizers. In this context, developing sustainable and economically efficient agricultural solutions becomes essential. The study presents the synthesis of an interpenetrating polymer network (IPN) of hydrogels by combining bacterial cellulose (BC) with poly(acrylic acid) crosslinked with N, N-methylene-bis-acrylamide (PAA–co–MBA) via free radical copolymerization. To explore their potential as bioactive compound carriers, an ethanolic hydroalcoholic phytoextract (EHP) obtained from Hypericum perforatum L. and Melissa officinalis L. was directly encapsulated within the IPN hydrogels. The EHP is valued for its rich bioactive profile and antifungal, antimycobacterial, and antioxidant properties. The results of rheology measurements and thermal gravimetric analysis (TGA) revealed that incorporating BC into the IPN hydrogels significantly enhanced the mechanical stiffness, thermal resistance, and overall stability of the resulting IPN structures. Fourier Transform Infrared (FTIR) spectroscopy and Scanning Electron Microscopy (SEM) confirmed the structural organization and the porosity of the developed composite, as well as the successful fabrication of IPN hydrogels in the EHP medium. Under optimal conditions, the IPN hydrogels exhibited a reduced swelling capacity, thereby slowing the diffusion of the bioactive agents, reducing the application frequency, and enhancing the utilization efficiency. Taken together with the controlled-release performance, these findings demonstrate the potential of BC (PAA-co-MBA) IPN hydrogels as biodegradable and sustainable carrier systems for controlled delivery applications and suggest that they may be promising candidates for hydrogel-based agricultural delivery systems. Full article

Background: Endometriosis is a chronic, estrogen-dependent inflammatory disorder associated with pain and immune dysregulation. Omega-3 (n-3) fatty acids and fish-derived bioactive lipids may modulate inflammation and metabolism. This study investigated whether adding fish-derived lipids (FDLs) to a Healthy Eating Plate diet improves inflammatory markers, gut inflammation, pain, and quality of life in women with endometriosis. Methods: In this 12-week randomized controlled trial, 46 women with confirmed endometriosis were assigned to either a Healthy Eating Plate diet alone (control group, CG) or the same diet plus FDLs (intervention group, IG). Primary outcomes included serum cytokine concentrations (interleukin [IL]-1β, IL-6, IL-8, IL-10, and tumor necrosis factor-alpha [TNF-α]) and fecal calprotectin (CAL). Secondary outcomes included pain intensity measured using the visual analog scale (VAS) and health-related quality of life assessed with the Endometriosis Health Profile-30 (EHP-30). Results: IL-10 levels (pg/mL) increased in the IG (32.5 ± 51.5 to 265 ± 508; p = 0.024) with a significant adjusted effect (ANCOVA: 5.07 [95% CI: 1.32–19.48], p = 0.019). Other cytokines showed heterogeneous responses, and CAL levels remained unchanged. EHP-30 Pain scores improved within both groups (CG p = 0.016; IG p = 0.002) without significant between-group change or adjusted effects (all p > 0.05). VAS scores decreased within the IG (5.0 ± 2.1 to 4.3 ± 2.1; p = 0.011), although there were no between-group differences in change and ANCOVA (all p > 0.05). However, ≥1-point responder rates were higher in the IG vs. CG (73.9% vs. 34.8%): adjusted RR = 2.11 (95% CI: 1.16; 3.89; p = 0.014). Conclusions: Overall, FDL supplementation was associated with a selected regulatory immune signal, reflected mainly by the IL-10 response, while most inflammatory, pain-related, and quality-of-life outcomes did not show consistent significant between-group effects. The observed greater likelihood of pain reduction in the FDL-supplemented group of women with endometriosis, along with the IL-10 response, should be interpreted with caution and confirmed in larger studies with longer follow-up/intervention time, taking into account the potential mechanisms of biological response. Full article

Background/Objectives: Melanoma affects both sexes, and its incidence has increased in recent years. It is currently among the most common types of cancer. Standard chemotherapy, although effective, often lacks selectivity for tumor cells, resulting in dose-limiting side effects. Electrochemotherapy and magnetic hyperthermia have been investigated as innovative biomedical approaches. Electrochemotherapy improves drug delivery by facilitating electroporation, thereby increasing intracellular concentrations of chemotherapeutic agents and reducing associated adverse effects. Furthermore, electroporation enhances sensitivity to magnetic hyperthermia. However, few studies have focused on the combination of electroporation and hyperthermia in melanoma models. This study aimed to evaluate the synergistic effects of intratumoral administration of superparamagnetic iron oxide nanoparticles (SPIONs), electroporation (EP), and magnetic hyperthermia (EHP) on fluorescent melanoma tumors generated with the MV3-GFP cell line. Methods: Fluorescent melanoma tumors were generated using the MV3-GFP cell line. Treatments included SPIONs alone, SPIONs combined with hyperthermia, and SPIONs combined with electroporation and hyperthermia. Tumor size was monitored over 21 and 28 days. Results: SPIONs alone did not affect tumor growth (665 mm³). SPIONs plus hyperthermia reduced tumor size to 126.5 mm³ at day 21. The combination of SPIONs, electroporation, and hyperthermia produced a pronounced antitumoral effect, with tumor size decreasing to 95.5 mm³ at day 14 and 6.8 mm³ at day 21, followed by complete tumor disappearance by day 28. Electroporation significantly enhanced the antitumoral activity of the combined treatment. Conclusions: The combination of SPIONs, electroporation, and magnetic hyperthermia shows significant synergistic antitumoral activity in a melanoma model. These findings support further investigation in larger and more comprehensive in vivo studies to better understand the therapeutic potential of these combined approaches. Full article

The microsporidian parasite Enterocytozoon hepatopenaei (EHP) is a major pathogen causing severe growth retardation in shrimp, leading to substantial economic losses in global aquaculture. To address the urgent need for accurate, rapid, and field-deployable diagnostic tools for EHP, this study developed a novel one-pot detection platform by integrating asymmetric Enzymatic Recombinase Amplification (aERA) with a PAM-independent CRISPR/Cas12a system (AYERA-Cas12a) based on ssDNA activation. This design circumvents the compatibility challenge between isothermal amplification and CRISPR activity in a single tube by generating single-stranded DNA amplicons that activate Cas12a without requiring a PAM sequence. The assay operates at a constant temperature of 46 °C and completes detection within 15 min. It achieves a sensitivity of 10 copies/μL, equivalent to qPCR, and shows no cross-reactivity with six other prevalent shrimp pathogens. Validation using 56 clinical shrimp (Litopenaeus vannamei, L. vannamei) samples demonstrated complete agreement with qPCR results. With its simple procedure, isothermal conditions, and clear endpoint fluorescence readout under blue light, the AYERA-Cas12a platform is suitable for point-of-care testing (POCT). This work provides a user-friendly tool for the on-site surveillance and early diagnosis of EHP, offering significant potential for improving disease management in shrimp farming. Full article

Environmental health services play a critical role in communicable disease outbreaks by addressing environmental determinants of disease transmission. However, the scope, impact, and challenges of Environmental Health Practitioner (EHP)-led interventions remain insufficiently documented. Aim and objectives: This systematic review objectively assessed the role, impacts, and challenges of municipal environmental health services in outbreak response, with a focus on South Africa, to inform the standardisation and strengthening of disease surveillance and prevention. Methods: The PICO framework guided the development of search terms and research questions. PubMed, Scopus, Google Scholar, and Web of Science were searched for English-language, full-text studies published between 2010 and 2024. Studies not meeting these inclusion criteria were excluded. Screening and reporting followed PRISMA guidelines, and data were synthesised using a standardised extraction tool. Results: A total of 58 studies were included. The key EHP functions identified were water quality monitoring, vector control, food safety, waste management, and outbreak response. While South Africa demonstrated comparatively advanced systems, persistent implementation challenges remain, including the integration of environmental monitoring with disease surveillance. The findings emphasised the need for integrating environmental monitoring with disease surveillance systems and integrating WASH and climate-responsive strategies. Conclusions and recommendation: The review recommends strengthening guidelines and advancing evidence-based practice. Enhancing EHP roles within surveillance frameworks is essential for improving outbreak preparedness and public health resilience. Full article

One approach to make the aviation sector climate-compatible is to minimize greenhouse gas emissions by employing electric and hybrid electric propulsion system concepts. The introduction of novel technologies introduces novel failure modes and consequently effects of failure conditions on the aircraft. This study examines the safety of distributed electrified aircraft propulsion systems and evaluates individual failure scenarios in the context of the relevant certification requirements. A comparison of the functional architectures of legacy and Electric Hybrid Propulsion Systems (EHPSs) is conducted and the existing aircraft-level requirements, that are based on experience with conventional propulsion systems, are assessed for their applicability to the certification of novel propulsion systems. Subsequently the relevant safety items from these requirements are identified in the context of a critical loss of thrust scenario. Analysis methods are assigned to these safety items in order to prove the compliance of the novel systems with the legacy certification documentation. This results in a validation concept for EHPS at the aircraft level in the context of a critical loss of thrust. In particular, the distribution of individual subsystems and components throughout the aircraft leads to reduced isolation of the respective propulsion systems and thus potential safety-critical interactions with adjacent systems. The analysis demonstrates that the use of distributed propulsion systems increases the risk of multiple failures of redundant systems and cascading failure propagation, highlighting the need to develop targeted means of prevention and the mitigation of failure conditions for these systems. Full article

Distributed electric propulsion (DEP) systems offer a wide range of options for arranging the propulsion units on an aircraft. In most cases, the position of the propulsion systems is optimized for one specific flight phase, e.g., takeoff or cruise. Taking advantage of the high lift potential of the DEP also during descent and approach phases represents a challenge due to increased thrust. Energy harvesting propellers (EHPs) can be used to adapt the resulting thrust, by generation an additional drag force while regenerating a certain amount of energy back into the system. Therefore, the scaled flight demonstrator (SFD) e-Genius-Mod was modified to implement an energy harvesting powertrain in a DEP system. The energy harvesting wingtip propellers are integrated in a pusher configuration. It is possible to investigate different operation modes for recuperation, such as Windmilling and Opposite Pitch, by adjusting different propeller pitch angles. The electronics used for the wingtip propellers (WTPs) enable the control and measurement of the recuperation performance and furthermore to charge recuperated energy back into the battery. The energy harvesting system was tested in a wind tunnel to verify its functionality. In Windmilling mode, the maximum mean electrical power output is −25.7 W. In Opposite Pitch mode, the values were significantly higher, with a maximum mean electrical power of −184 W. This corresponds to up to seven times as much regenerated power in Opposite Pitch mode. Full article

This study evaluated the effects of including spray-dried porcine plasma (SDP) in nursery diets and enzymatically hydrolyzed plasma (EHP) in drinking water on piglet growth performance and post-weaning diarrhea (PWD). Four treatments were tested: CONTROL (soy protein concentrate, SPC), P1SDP (5% SDP in phase 1), P1 + P2SDP (5% SDP and 2% SDP in phases 1 and 2), and EHP (0.88% in water during phases 1 and 2). No significant differences among treatments were observed during phase 1. During phase 2 (14–28 days) pigs fed SDP or pigs provided EHP in water had higher average daily gain (ADG; p = 0.001) and feed conversion (GFR; p = 0.013) versus the other groups. Pigs fed SDP in the first two phases had an average d-42 body weight that was 1.54 kg heavier than controls. Post-weaning diarrhea was not observed at any time during the study. These results support the use of SDP and EHP as effective nutritional strategies to enhance the growth and resilience of pigs during the post-weaning period. Both ingredients contribute to sustainable pig production by improving efficiency and promoting circular economy practices through the valorization of animal by-products. Full article

Enterocytozoon hepatopenaei (EHP) is a specialized parasitic microsporidian that causes significant economic losses to the shrimp farming industry. The glycolysis pathway plays an important role in the survival of EHP spores in vitro. In this study, key enzyme genes involved in the glycolysis pathway of EHP were analyzed, and purified spores were treated with KOH and a low temperature (−20 °C) to promote or inhibit germination. Quantitative analysis and enzyme activity of the initiating key gene hexokinase (HK) and the core link gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were conducted to explore the energy response characteristics of germinating spores. The results showed that HK and GAPDH genes had significant differences from the host, based on phylogenetic analysis. The expression of HK gene and enzyme activity increased after promoting germination treatment. The expression of the GAPDH gene was stable, but the activity of the GAPDH enzyme increased significantly after germination promotion. These findings indicate that the inhibition of the HK gene expression level and GAPDH protein level can block spore germination and invasion in vitro, which could be used as potential control targets for EHP. Full article

The electro-hydraulic pump (EHP), as the primary power component of the electro-hydrostatic actuator, typically operates in a wet environment filled with hydraulic oil, thereby experiencing vibration response alterations due to the added mass of the fluid. Accurate identification of the wet modal characteristics is essential for improving the fidelity of electromagnetic vibration prediction in EHPs. In this work, an integrated EHP is investigated. A finite-element model is established to perform dry and wet modal analyses, from which the first nine natural frequencies and associated mode shapes are extracted. Dry and wet experimental modal tests are then conducted using an impact-hammer setup to validate the numerical model. The results indicate a systematic reduction in natural frequencies under oil-filled conditions, with more pronounced shifts in the lower-order modes; a maximum decrease of 10.92% is observed. On this basis, the stator tooth electromagnetic forces are obtained from two-dimensional electromagnetic finite-element simulations, and vibration responses are predicted via modal superposition using either dry or wet modal parameters. Finally, vibration measurements are performed under oil-filled operating conditions. The measured spectra exhibit pronounced tonal components at the electrical fundamental frequency and its even harmonics, and wet modal-based electromagnetic vibration prediction improves the accuracy by 78.90% relative to the dry modal-based prediction. These findings provide both theoretical support and practical guidance for low-vibration and low-noise design of EHPs. Full article

This 90-day trial evaluated the integrated benefits of biofloc technology (BFT) in lined ponds for intensive Penaeus vannamei culture, comparing it with a conventional water-exchange (WE) system. The BFT system maintained favorable water quality with a 68.4% reduction in cumulative water exchange. Concentrations of toxic total ammonia–nitrogen (TAN) and nitrite–nitrogen (NO₂⁻-N) were better controlled, and total suspended solids (TSS) stabilized within a beneficial range (150–200 mg L⁻¹). Microbial analysis indicated that BFT significantly increased total bacterial abundance in both culture water and shrimp hepatopancreas while reducing the total Vibrio-to-bacteria ratio in culture water to below 6%, significantly lower than in the WE system (>18%). Moreover, BFT significantly lowered the loads of specific pathogens, acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus, and Enterocytozoon hepatopenaei (EHP) in both culture water and shrimp hepatopancreas. Regarding production performance, BFT significantly enhanced shrimp survival rate (82.4% vs. 71.5%), yield (3460 vs. 2948 kg pond⁻¹), and water productivity (0.85 vs. 0.28 kg m⁻³), while lowering the feed conversion ratio (1.16 vs. 1.33). In conclusion, BFT achieves stable water quality, effective pathogen suppression, and enhanced production efficiency through microbial regulation, offering a viable water-saving, environmentally sound, and disease-resilient strategy for intensive P. vannamei culture. Full article

This essay poses a novel integrative environmental health promotion (EHP) framework inclusive of spiritual tenets to increase interdisciplinary science as well as public engagement for improved people, place, and planetary (3P) health outcomes. Environmental public health professionals have typically relied upon quantitative scientific evidence related to negative human health outcomes from toxic exposures. Environmental health lags behind more progressive mixed-methods research frameworks leveraging health promotion and 3P health initiatives. This essay argues for a novel integrative EHP framework to encourage more mixed-methods research based on merging an integrative health (body-mind-spirit) perspective and the public health ecological model. Using a three-dimensional Cartesian Coordinate System, the author developed a visual integrative EHP framework with the future ability to record, interpret, and report data with units of measure in three dimensions rather than the traditional x- and y-axis variable relationships. The long-term goal is to engage researchers, study participants, and the general public in exploring new 3P health research and outcomes inclusive of the spiritual axis to leverage more scientific evidence for the care and nurturing of our common home as a basic tenet of civil society. Full article

Communicable diseases remain a significant public health burden in South Africa, particularly where environmental determinants of health intersect with fragmented surveillance systems. Environmental Health Practitioners (EHPs) are legally mandated to implement the surveillance and prevention of communicable disease services at the municipal level. However, this function is inconsistently operationalised and often remains reactive (outbreak-driven), with limited integration into broader national surveillance systems. This study protocol outlines a mixed-methods investigation to develop a practical framework to strengthen the communicable disease surveillance and prevention function within Environmental Health Services in South Africa. The study will assess existing guiding tools, operational practices, and intersectoral collaboration mechanisms supporting surveillance across metropolitan and district municipalities. Quantitative data will be collected through a national survey of EHPs, while qualitative data will be generated through key informant interviews with national stakeholders, focus group discussions with municipal health managers, and a targeted review of municipal documents. Quantitative data will be analysed using descriptive and inferential statistics, while qualitative data will be thematically analysed and triangulated across data sources. The expected outcome is an integrated framework that clarifies roles, strengthens data flow, and promotes proactive, coordinated surveillance and prevention of communicable diseases within environmental health. The developed framework is anticipated to inform policy discussions and may contribute to efforts aligned with Sustainable Development Goal 3, Target 3.3, on reducing communicable disease burdens, by strengthening municipal communicable disease surveillance and prevention. Full article

Environmental Health Practitioners (EHPs) play a critical regulatory role within municipal health services, yet their occupational health and safety (OHS) risks remain poorly characterised in the literature. This narrative review synthesises evidence on the physical, biological, chemical, ergonomic, and psychosocial hazards encountered by EHPs during routine municipal inspection and enforcement activities. A structured literature search across major databases was conducted, and findings were synthesised using a risk characterisation framework to examine hazard types, exposure pathways, and associated health outcomes. The review demonstrates that EHPs are exposed to simultaneous and interacting hazards through multiple routes, including inhalation, dermal contact, ingestion, traumatic incidents, and psychosocial strain. Where available, quantitative indicators of exposure magnitude from inspection-relevant environments were identified, highlighting both potential risk severity and significant gaps in direct exposure measurement. Importantly, the findings indicate that occupational risks faced by EHPs are largely systemic, shaped by organisational constraints, resource limitations, enforcement contexts, and broader governance conditions rather than isolated individual behaviours. This review contributes to safety science by providing an integrated conceptual model of EHP occupational exposure pathways and by highlighting the need for system-level OHS interventions within municipal health services. Strengthening PPE provision, organisational support, and exposure monitoring is essential to improving EHPs safety and the effectiveness of environmental health regulation. Full article

Litopenaeus vannamei, a widely cultivated aquatic species worldwide, has its growth status intrinsically tied to the economic profitability of the aquaculture industry. However, infection by the microspondian parasite Enterocytozoon hepatopenaei (EHP) has emerged as a pivotal threat to the healthy growth of these shrimp. Through transcriptome sequencing, this study identified substantial alterations in gene expression patterns related to growth regulation, immune system activation, and energy metabolism regulation. Specifically, in L. vannamei shrimp exhibiting normal growth, the elevated expression of genes such as CYP450, PPAF, FASN and ACSBG2 serves as molecular indicators of their growth superiority and resistance to parasitic infection. Furthermore, metabolome analysis revealed distinct changes in lipid and nucleotide metabolic pathways, offering valuable insights into the metabolic disruptions caused by infection. The integrated analysis of transcriptome and metabolome data indicated a notable positive correlation between the differentially expressed genes TPi and ALF-like, and the metabolites acetyl-L-carnitine and citric acid. This suggests potential synergistic mechanisms in regulating energy metabolism and immune responses against EHP infection. These findings enhance our comprehension of EHP infection mechanisms and establish a scientific groundwork for developing more precise and efficacious prevention and control strategies, ultimately promoting the healthy growth of L. vannamei and ensuring the sustainable development of the aquaculture industry. Full article