Search Results (16,052)

The current study focuses on a preliminary evaluation of the use of solid residues produced from the distillation of selected medicinal and aromatic plants (MAP) as fertilizers for alkaline soils. Specifically, the residues of hemp (Cannabis sativa L.), helichrysum (Helichrysum Italicum (Roth) G. Don), lavender (Lavandula angustifolia Mill.), oregano (Origanum vulgare L.), rosemary (Rosmarinus officinalis L.) and sage (Salvia officinalis L.) were added in an alkaline and calcareous soil at the rates of 0 (control), 1, 2, 4 and 8%, in three replications (treatments), and the treated soils were analyzed. The results showed that upon application of the residues, soil electrical conductivity (EC), organic C, total N and the C/N ratio significantly increased, especially at the 4 and 8% rates. The same was found for soil available P, K, B, Cu and Mn. The effects of the residues on soil pH, cation exchange capacity (CEC) and available Zn and Fe were rather inconclusive, whereas soil available N significantly decreased, which was somewhat unexpected. From the different application rates tested, it seems that all residues could improve soil fertility (except N?) when they were applied to soil at rates of 2% and above, without exceeding the 8% rate. The reasons for the latter statement are soil EC and available Mn: the doubling of EC upon application of the residues and the excessive increase in soil available Mn in treatments with 8% residues raise concerns of soil salinization and Mn phytotoxicity risks, respectively. This work provides the first step towards the potential agronomic use of solid residues from MAP distillation in alkaline soils. However, for the establishment of such a perspective, further research is needed in respect to the effect of residues on plant growth and soil properties, by means of at least pot experiments. Based on the results of the current study, the undesirable effect of residues on soil available N should be investigated in depth, since N is the most important essential element for plant growth, and possible risks of micronutrient phytotoxicities should also be studied. In addition, application rates between 2 and 4% should be studied extensively in order to recommend optimum application rates of residues to producers. Full article

Against the backdrop of an urgent energy crisis, solar energy has attracted sufficient attention as one of the most inexhaustible and friendly types of environmental energy. Faced with long service and harsh environment, the poor performance ratios of photovoltaic arrays and safety hazards are frequently boosted worldwide. In particular, the hot spot effect plays a vital role in weakening the power generation performance and reduces the lifetime of photovoltaic (PV) modules. Here, our research reports a spatial–temporal hot spot management system integrated with fiber Bragg grating (FBG) temperature sensor arrays and cooling hydrogels. Through finite element simulations and indoor experiments in laboratory conditions, a superior cooling effect of hydrogels and photoelectric conversion efficiency improvement have been demonstrated. On this basis, field tests were carried out in which the FBG arrays detected the surface temperature of the PV module first, and then a classifier based on an optimized artificial neural network (ANN) recognized hot spots with an accuracy of 99.1%. The implementation of cooling hydrogels as a feedback mechanism achieved a 7.7 °C reduction in temperature, resulting in a 5.6% enhancement in power generation efficiency. The proposed strategy offers valuable insights for conducting predictive maintenance of PV power plants in the case of hot spots. Full article

The composition and structure of understory plants are crucial for forest ecosystem succession and stability. This study examined the impact of various Cunninghamia lanceolata mixed plantation patterns on understory biodiversity, aiming to provide a theoretical foundation for sustainable management. Six patterns were evaluated using sample plots at Guanshan Forest Farm in Jiangxi Province, China. Understory vegetation diversity, biomass, and soil properties—including total nitrogen, available nitrogen, total phosphorus, available phosphorus, total potassium, available potassium, soil organic matter, and pH—were quantitatively analyzed. Significant differences in diversity among the patterns were revealed. The ‘Cunninghamia lanceolata + Phoebe bournei (Hemsl.) Yen C. Yang + Schima superba Gardner & Champ’ mixed plantation exhibited the most pronounced enhancement of understory plant diversity, whereas the ‘C. lanceolata + Liquidambar formosana Hance’ pattern demonstrated the least significant effects among all treatments. Significant correlations were detected between soil nutrients and diversity indices. Mixed patterns enhance diversity through expanded ecological niches and optimized microenvironments, thereby strengthening ecological functions and management efficiency. Full article

Chimonanthus praecox, a traditional ornamental plant in China, is admired for its ability to bloom during the cold winter season and is recognized as an outstanding woody cut flower. MADS-box genes encode transcription factors essential for plant growth and development, with key functions in regulating flowering time and the formation of floral organs. In this study, 74 MADS-box genes (CpMADS1–CpMADS74) were identified and mapped across 11 chromosomes, with chromosome 1 harboring the highest number (13 genes) and chromosome 3 the fewest (3 genes). Physicochemical property analysis revealed that all CpMADS proteins are hydrophilic and predominantly nuclear-localized. Phylogenetic analysis classified these genes into Type I and Type II subfamilies, highlighting a clear divergence in domain structure. Eighty simple sequence repeat (SSR) loci were detected, with dinucleotide repeats being the most abundant, and the majority located in Type II MADS genes. From 23 C. praecox samples, 10 polymorphic SSR markers were successfully developed and PCR-validated, enabling a cluster analysis that grouped these cultivars into three distinct clusters. This study offers significant insights into the regulation of flowering, floral organ development, genetic linkage map construction, and the application of marker-assisted selection in C. praecox. Full article

With increasing interest in natural therapeutic strategies for skin aging, plant-derived compounds have gained attention for their potential to protect against oxidative stress and inflammation. In this study, we investigated the anti-aging and anti-inflammatory effects of flavonoids isolated from Cercidiphyllum japonicum using a tumor necrosis factor-alpha (TNF-α)-stimulated normal human dermal fibroblast (NHDF) model. The aerial parts of C. japonicum were extracted and analyzed by high-performance liquid chromatography (HPLC), leading to the identification of four major compounds: maltol, chlorogenic acid, ellagic acid, and quercitrin. Each compound was evaluated for its antioxidant and anti-aging activities in TNF-α-stimulated NHDFs. Among them, ellagic acid exhibited the most potent biological activity and was selected for further mechanistic analysis. Ellagic acid significantly suppressed intracellular reactive oxygen species (ROS) generation and matrix metalloproteinase-1 (MMP-1) secretion (both p < 0.001), while markedly increasing type I procollagen production (p < 0.01). Mechanistic studies demonstrated that ellagic acid inhibited TNF-α-induced phosphorylation of mitogen-activated protein kinases (MAPKs), downregulated cyclooxygenase-2 (COX-2), and upregulated heme oxygenase-1 (HO-1), a key antioxidant enzyme. Additionally, ellagic acid attenuated the mRNA expression of inflammatory cytokines, including interleukin-6 (IL-6) and interleukin-8 (IL-8), indicating its broad modulatory effects on oxidative and inflammatory pathways. Collectively, these findings suggest that ellagic acid is a promising plant-derived bioactive compound with strong antioxidant and anti-inflammatory properties, offering potential as a therapeutic agent for the prevention and treatment of skin aging. Full article

The sustainable utilization of saline water resources represents an effective strategy for alleviating water scarcity in arid regions. However, the mechanisms by which prolonged saline water irrigation influences soil salinization and dryland crop growth are not yet fully understood. This study examined the effects of six irrigation water salinity levels (CK: 0.87 g·L⁻¹, S1: 2 g·L⁻¹, S2: 4 g·L⁻¹, S3: 6 g·L⁻¹, S4: 8 g·L⁻¹, S5: 10 g·L⁻¹) on soil salinization dynamics and jujube growth during a three-year field experiment (2020–2022). The results showed that soil salinity within the 0–1 m profile significantly increased with rising irrigation water salinity and prolonged irrigation duration, with the 0–0.4 m layer accounting for 50.27–74.95% of the total salt accumulation. A distinct unimodal salt distribution was observed in the 0.3–0.6 m soil zone, with the salinity peak shifting downward from 0.4 to 0.5 m over time. Meanwhile, soil pH and sodium adsorption ratio (SAR) increased steadily over the study period. The dominant hydrochemical type shifted from SO₄²⁻-Ca²⁺·Mg²⁺ to Cl⁻-Na⁺·Mg²⁺. Crop performance exhibited a nonlinear response to irrigation salinity levels. Low salinity (2 g·L⁻¹) significantly enhanced plant height, stem diameter, leaf area index (LAI), vitamin C content, and yield, with improvements of up to 12.11%, 3.96%, 16.67%, 16.24%, and 16.52% in the early years. However, prolonged exposure to saline irrigation led to significant declines in both plant growth and water productivity (WP) by 2022. Under high-salinity conditions (S5), yield decreased by 16.75%, while WP declined by more than 30%. To comprehensively evaluate the trade-off between economic effects and soil environment, the entropy weight TOPSIS method was employed to identify S1 as the optimal irrigation treatment for the 2020–2021 period and control (CK) as the optimal treatment for 2022. Through fitting analysis, the optimal irrigation water salinity levels over 3 years were determined to be 2.75 g·L⁻¹, 2.49 g·L⁻¹, and 0.87 g·L⁻¹, respectively. These findings suggest that short-term irrigation of jujube trees with saline water at concentrations ≤ 3 g·L⁻¹ is agronomically feasible. Full article

The growth potential of Crinum bulbispermum was evaluated on gold mine tailings. The primary objectives were to model the species’ climatic niche in relation to gold mining regions, assess its germination success on tailings, and compare seedling survival and growth on tailings versus other soil types. Species distribution modelling identified the South African Grassland Biome on the Highveld (1000+ m above sea level), where the majority of gold mines are located, as highly suitable for the species. Pot trials demonstrated above 85% germination success across all soil treatments, including gold mine tailings, indicating its potential for restoration through direct seeding. An initial seedling establishment rate of 100% further demonstrated the species’ resilience to mine tailings, which are often seasonally dry, nutrient-poor, and may contain potentially toxic metals. However, while C. bulbispermum was able to germinate and establish in mine tailings, long-term growth potential (over 12 months) was constrained by low organic carbon content (0.11%) and high salinity (194.50 mS/m). These findings underscore the critical role of soil chemistry and organic matter in supporting long-term plant establishment and growth on gold tailings. Building on previous research, this study confirms the ability of this thick-rooted geophyte to tolerate chemically extreme soil conditions. Crinum bulbispermum shows promise for phytostabilization and as a potential medicinal plant crop on tailings. However, future research on microbial community interactions and soil amendment strategies is essential to ensure its long-term sustainability. Full article

The apoplast is often the first point of contact between plant cells and invading pathogens, serving as an important site for defense signaling. Pokeweed antiviral protein (PAP), a ribosome-inactivating protein from Phytolacca americana (pokeweed), is localized to the apoplast and is hypothesized to accompany a pathogen to the cytosol, where it would inactivate host ribosomes to prevent pathogen spread. However, it is not known whether PAP interacts with other proteins in the apoplast. In this study, we identified Phytolacca americana cysteine protease 1 (PaCP1), an extracellular cysteine protease, as a novel PAP interactor. Sequence and structural analyses classified PaCP1 as a member of the C1A subfamily of papain-like cysteine proteases. Immunoprecipitation, mass spectrometry, and yeast two-hybrid analysis showed that PAP specifically binds the mature, active form of PaCP1. Curiously, PaCP1 cleaves PAP at its N- and C-termini, generating peptides that enhance MAPK phosphorylation in pokeweed leaves, indicating their potential role in stress signaling. PaCP1 processing of PAP to generate bioactive peptides diversifies the function of a ribosome-inactivating protein beyond its canonical inhibition of translation. Our findings present a novel extracellular role for PAP and advance our understanding of how protein interactions in the apoplast contribute to plant immune responses. Full article

Atriplex hortensis var. rubra (red orache, RO) is a halotolerant species rich in nutraceutical compounds, which makes it a valuable crop for human nutrition. This plant could also be exploited for phytoremediation of contaminated soil and wastewater, and for saline aquaponics. A root rot disease was observed on hydroponically grown RO plants, caused by Pythium deliense and Pythium Cluster B2a sp. Identification was based on morphology, molecular analysis (ITS and COI), and phylogenetic analysis. We assessed disease severity in plants grown in a growth chamber with nutrient solutions containing different NaCl concentrations (0, 7, and 14 g L⁻¹ NaCl). In vitro growth at different salinity levels and temperatures was also evaluated. Both Pythium species were pathogenic but showed different responses. Pythium deliense was significantly more virulent than Pythium Cluster B2a sp., causing a steady reduction in root dry weight (RDW) of 70% across all salinity levels. Pythium Cluster B2a sp. reduced RDW by 50% at 0 and 7 g L⁻¹ NaCl while no symptoms were observed at 14 g L⁻¹ NaCl. Pythium deliense grew best at 7 and 14 g L⁻¹ NaCl, while Pythium Cluster B2a sp. growth was reduced at 14 g L⁻¹ NaCl. Both pathogens had an optimum temperature of 30 °C. This is the first report of Pythium spp. causing root rot on RO grown hydroponically. The effective use of halophytic crops must consider pathogen occurrence and fitness in saline conditions. Full article

Tea polyphenol oxidase (CsPPO) is a crucial enzyme involved in the production of tea and tea products. However, the recombinant expression of CsPPO in microorganisms is often hindered by challenges such as inclusion body formation and extremely low enzyme activity. In this study, the CsPPO gene (1800 bp) from Camellia sinensis cv. Yihongzao was cloned and 14.5% of its codons were optimized for Pichia pastoris expression. Compared to pre-optimization, codon optimization significantly enhanced CsPPO production in P. pastoris KM71, yielding a 42.89-fold increase in enzyme activity (1286.67 U/mL). The optimal temperature and pH for recombinant CsPPO were determined to be 40 °C and 5.5, respectively. This study demonstrates that codon optimization effectively improves the expression of plant-derived enzymes such as CsPPO in eukaryotic expression systems. Future research should explore the long-term stability of recombinant CsPPO and its potential applications in tea fermentation processes and functional food development. Full article

The induction of type I interferon (IFN) via intracellular nucleic acid sensors may be useful in preventing viral infections. However, little is known about the effect of natural plant materials on sensor responses. We previously found that cardamom (Elettaria cardamomum (L.) Maton) seed extract (CSWE) enhanced type I IFN expression and prevented influenza virus infection. In this study, we investigated the effect of CSWE on type I IFN responses using intracellular nucleic acid sensor molecules. Human lung epithelial A549 cells were treated with CSWE and transfected with poly(dA:dT) or poly(I:C) using lipofection. CSWE and 1,8-cineole, the major CSWE components, dose-dependently induced type I IFNs and IFN-stimulated genes in both poly(dA:dT)- and poly(I:C)-transfected A549 cells. The type I IFN-enhancing effect of CSWE was dependent on the stimulator of interferon genes (STING), whereas the effect of 1,8-cineole was independent of STING and mediated by the down-regulation of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-inducible poly-ADP-ribose polymerase expression. Our study suggests that CSWE has the potential to act as a beneficial antiviral agent by enhancing homeostatic type I IFN production. Full article

Proteomic profiling using ultrafast chromatography–mass spectrometry provides valuable insights into plant responses to abiotic factors by linking molecular changes with physiological outcomes. Nanopriming, a novel approach involving the treatment of seeds with nanoparticles, has demonstrated potential for enhancing plant metabolism and productivity. However, the molecular mechanisms underlying nanoparticle-induced effects remain poorly understood. In this study, we investigated the impact of gold nanoparticle (Au-NP) seed priming on the proteome of wheat (Triticum aestivum L.) seedlings. Differentially regulated proteins (DRPs) were identified, revealing a pronounced reorganization of the photosynthetic apparatus (PSA). Both the light-dependent reactions and the Calvin cycle were affected, with significant upregulation of chloroplast-associated protein complexes, including PsbC (CP43), chlorophyll a/b-binding proteins, Photosystem I subunits (PsaA and PsaB), and the γ-subunit of ATP synthase. The large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) exhibited over a threefold increase in expression in Au-NP-treated seedlings. The proteomic changes in the large subunit RuBisCo L were corroborated by transcriptomic data. Importantly, the proteomic changes were supported by physiological and biochemical analyses, ultrastructural modifications in chloroplasts, and increased photosynthetic activity. Our findings suggest that Au-NP nanopriming triggers coordinated molecular responses, enhancing the functional activity of the PSA. Identified DRPs may serve as potential biomarkers for further elucidation of nanopriming mechanisms and for the development of precision strategies to improve crop productivity. Full article

Grafting serves as a crucial propagation technique for superior Camellia oleifera varieties, where rootstock–scion compatibility significantly determines survival and growth performance. To systematically evaluate grafting compatibility in this economically important woody oil crop, we examined 15 rootstock–scion combinations using ‘Xianglin 210’ as the scion, assessing growth traits and conducting physiological assays (enzymatic activities of SOD and POD and levels of ROS and IAA) at multiple timepoints (0–32 days post-grafting). The results demonstrated that Comb. 4 (Xianglin 27 rootstock) exhibited superior compatibility, characterized by systemic antioxidant activation (peaking at 4–8 DPG), rapid auxin accumulation (4 DPG), and efficient sugar allocation. Transcriptome sequencing and WGCNA analysis identified 3781 differentially expressed genes, with notable enrichment in stress response pathways (Hsp70, DnaJ) and auxin biosynthesis (YUCCA), while also revealing key hub genes (FKBP19) associated with graft-healing efficiency. These findings establish that successful grafting in C. oleifera depends on coordinated rapid redox regulation, auxin-mediated cell proliferation, and metabolic reprogramming, with Comb. 4 emerging as the optimal rootstock choice. The identified molecular markers not only advance our understanding of grafting mechanisms in woody plants but also provide valuable targets for future breeding programs aimed at improving grafting success rates in this important oil crop. Full article

The extensive use of pesticides has significant implications for public health and the environment. Breeding crop plants is the most effective and environmentally friendly approach to improve the plants’ resistance. However, it is time-consuming and costly, and it is sometimes difficult to achieve satisfactory results. Plants induce defense responses to natural elicitors by interpreting multiple genes that encode proteins, including enzymes, secondary metabolites, and pathogenesis-related (PR) proteins. These responses characterize systemic acquired resistance. Humic substances trigger positive local and systemic physiological responses through a complex network of hormone-like signaling pathways and can be used to induce biotic and abiotic stress resistance. This study aimed to assess the effect of humic substances on the activity of phenylalanine ammonia-lyase (PAL), peroxidase (POX), and β-1,3-glucanase (GLU) used as a resistance marker in various plant species, including orange, coffee, sugarcane, soybeans, maize, and tomato. Seedlings were treated with a dilute aqueous suspension of humic substances (4 mM C L⁻¹) as a foliar spray or left untreated (control). Leaf tissues were collected for enzyme assessment two days later. Humic substances significantly promoted the systemic acquired resistance marker activities compared to the control in all independent assays. Overall, all enzymes studied in this work, PAL, GLUC, and POX, showed an increase in activity by 133%, 181%, and 149%, respectively. Among the crops studied, citrus and coffee achieved the highest activity increase in all enzymes, except for POX in coffee, which showed a decrease of 29% compared to the control. GLUC exhibited the highest response to HS treatment, the enzyme most prominently involved in increasing enzymatic activity in all crops. Plants can improve their resistance to pathogens through the exogenous application of HSs as this promotes the activity of enzymes related to plant resistance. Finally, we consider the potential use of humic substances as a natural chemical priming agent to boost plant resistance in agriculture Full article

High nighttime temperature (HNT) due to asymmetric diurnal warming threatens wheat productivity. This study evaluated the effect of HNT on wheat phenology, physiology, and yield through field and controlled environment experiments in Central Queensland, Australia. Two wheat genotypes, Faraday and AVT#6, were assessed under three sowing dates—1 May (Early), 15 June (Mid), and 1 August (Late)—within the recommended sowing window for the region. In a parallel growth chamber study, the plants were exposed to two nighttime temperature regimes, of 15 °C (normal) and 20 °C (high), with consistent daytime conditions from booting to maturity. Late sowing resulted in shortened vegetative growth and grain filling periods and increased exposure to HNT during the reproductive phase. This resulted in elevated floret sterility, lower grain weight, and up to 40% yield loss. AVT#6 exhibited greater sensitivity to HNT despite maturing earlier. Leaf gas exchange analysis revealed increased nighttime respiration (Rn) and reduced assimilation (A), resulting in higher Rn/A ratio for late-sown crops. The results from controlled environment chambers resembled trends of the field experiment, producing lower grain yield and biomass under HNT. Cumulative nighttime hours above 20 °C correlated more strongly with yield losses than daytime heat. These findings highlight the need for HNT-tolerant genotypes and optimized sowing schedules under future climate scenarios. Full article