Enzymes in Food Industry: Fermentation Process, Properties, Rational Design and Applications: 2nd Edition

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Biotechnology".

Deadline for manuscript submissions: 30 June 2025 | Viewed by 1719

Special Issue Editors


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Guest Editor
College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
Interests: enzyme; functional food; rational design; molecular simulation
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Guest Editor
Manchester Institute of Biotechnology, University of Manchester, Manchester M1 7DN, UK
Interests: metalloenzymes; biocatalysis; protein engineering; enzyme mechanisms
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Volume I of this Special Issue (https://www.mdpi.com/journal/foods/special_issues/J114TEX953) was an amazing success, receiving widespread attention and interest from many scholars.  I would like to take this opportunity to thank them for their contributions and support.  This topic continues to play a pivotal role in the field of food today, and I believe that now is the perfect time to launch Volume II.  I hope it will be as successful as Volume I and have clear significance and value for this field.

As common bio-macromolecular catalysts, enzymes are widely used in food preparation to improve food products' taste and texture. More importantly, enzymes perform crucial roles in producing bioactive compounds, important food ingredients and some new nutritional ingredients, which can improve the food quality and function. In this Special Issue, we welcome original research or review manuscripts that cover the utility of free enzymes and/or immobilized enzymes as efficient tools for various aspects of food industries. Specifically, the focus will be on novel enzyme mining, enzyme structure–function relationship, and enzymes' immobilization and modification. In addition, developing novel enzymes in creating new food components and rational design of food enzymes are strongly encouraged.

Prof. Dr. Fufeng Liu
Dr. Jingming Zhao
Guest Editors

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Keywords

  • enzyme engineering
  • enzyme
  • food industry
  • enzyme activity
  • enzyme immobilization
  • biocatalysts
  • enzymatic modification
  • enzyme design
  • food ingredients
  • functional food
  • bioactive compounds
  • enzyme stability
  • molecular simulation
  • structure–function relationship

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Published Papers (1 paper)

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Research

20 pages, 8970 KiB  
Article
Ultrasound-Assisted Enzymatic Extraction of Polysaccharides from Tricholoma matsutake: Optimization, Structural Characterization, and Inhibition of α-Synuclein Aggregation
by Wen Gao, Yang Wang, Fuping Lu and Fufeng Liu
Foods 2024, 13(24), 4150; https://doi.org/10.3390/foods13244150 - 21 Dec 2024
Cited by 1 | Viewed by 1285
Abstract
This study optimized ultrasound-assisted enzymatic (UAE) extraction of TMP (Tricholoma matsutake polysaccharide) through response surface methodology. The optimal conditions included complex enzyme comprising 1.15% cellulase, 0.60% pectinase, and 0.95% dispase, with ultrasound for 24 min at 84.5 °C and enzyme hydrolysis at [...] Read more.
This study optimized ultrasound-assisted enzymatic (UAE) extraction of TMP (Tricholoma matsutake polysaccharide) through response surface methodology. The optimal conditions included complex enzyme comprising 1.15% cellulase, 0.60% pectinase, and 0.95% dispase, with ultrasound for 24 min at 84.5 °C and enzyme hydrolysis at pH 5.0. This process yielded 19.74 ± 0.51% TMP, exceeding traditional hot water extraction by over four times. Fourier transform infrared spectroscopy (FT–IR) confirmed that UAE did not alter the structure of TMP. In vitro experiments indicated that TMP-UAE demonstrated enhanced antioxidant properties. Further purification through DEAE-52 and Sephadex G-100 chromatography resulted in a homogenous polysaccharide fraction (TMP). Characterization indicated that TMP has an average molecular weight of 2.79 × 104 Da, composed of fucose, galactose, glucose and mannose in a 2.00:9.44:86.29:2.28 molar ratio. FT–IR indicated the presence of C-O-C glycosidic bonds and pyranyl-type sugar rings. Scanning electron microscopy displayed loose lamellar structures with small pores. Finally, TMP exhibited therapeutic potential against C. elegans in Parkinson’s disease, including reducing α-synuclein aggregation, protecting dopaminergic neurons, and prolonging lifespan. This study provides an efficient extraction method for TMP and an insight into its neuroprotective effect in PD C.elegans. Full article
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