Search Results (15)

Diaporthe amygdali Delacr. is a phytopathogenic fungus of considerable agronomic importance, responsible for branch canker in almond (Prunus dulcis [Mill.] D.A. Webb) and peach (Prunus persica L.) trees. It represents a major phytosanitary threat to almond cultivation in Europe, particularly in Mediterranean regions. Almond is currently among the most rapidly expanding perennial crops, with cultivated areas increasing as a result of the introduction of new cultivars and the adoption of improved agronomic practices. The objectives of this study were to isolate and identify fungal pathogens from infected almond samples collected in France through multilocus phylogenetic analyses (ITS, tef1-α, his3, tub2, cal genes) combined with morphological characterization; evaluate the susceptibility of 18 almond genotypes, using ‘Ferragnès’ and ‘Texas’ as reference standards for susceptibility and tolerance, respectively; and compare three field inoculation methods. All isolates were identified as D. amygdali. The varietal screening identified marked differences in resistance among the tested cultivars. In particular, ‘Ferrastar’, ‘R1877’, ‘R1413’, and ‘R1542’ exhibited high levels of resistance, whereas ‘Tuono’, ‘Guara’, and ‘R1568’ showed susceptibility comparable to that of ‘Ferragnès’, which was used as the susceptible control. Among the inoculation methods evaluated, the mycelial plug technique proved to be the most consistent and reliable, outperforming both conidial suspension inoculation and the toothpick method coated with mycelium. These findings further confirm the genetic resistance of the cultivars ‘Ferrastar’ and ‘Ardèchoise’ to branch canker across different growing conditions, supporting their suitability for use in breeding and genetic improvement programs. Full article

Almond cultivation in the Mediterranean basin is driven by Spanish production, which has combined innovation in cultivation techniques with research on varietal improvement, releasing self-compatible and late-flowering varieties that are better suited to areas prone to spring frosts. The growing demand for almonds has also influenced the Italian nut market, pushing growers towards almond cultivation even in areas that historically have no full vocation due to certain climatic constraints, such as cold winters and spring frosts, which are now less severe and less frequent as a result of climate changes, such as along the Latium coastline in Central Italy. In this new regional context, an almond collection orchard was set up in late 2017 in the rural environment of the municipality of Tarquinia (province of Viterbo) to test the local adaptation of commercial almond cultivars. Phenological observations and data acquisition were conducted for three consecutive growing seasons (2022–2024) and referred to eight cultivars introduced in the orchard: namely Genco, Guara, Lauranne Avijour, Penta, Soleta, Supernova, Tuono, and Vialfas. Each accession was monitored for phenological and ecophysiological traits, plant vigor and yield, yield efficiency, and nut and kernel traits. The trial proved that Lauranne, Penta, and Vialfas, due to their late flowering, were able to avoid possible damage from the spring cold recorded in 2023, while Supernova, Penta, and Genco, with an average yield over the three-year period of 2.51 ± 1.53, 2.44 ± 1.07 and 2.69 ± 1.42 kg plant⁻¹, respectively, showed a higher average yield than the other cultivars tested. Full article

One of the key techniques for successful almond tree cultivation in newly irrigated areas is increasing planting density. To investigate this, field experiments were carried out over five consecutive growing seasons (2019–2023) to evaluate the effects of two different tree densities on the vegetative growth and productivity of almond trees (Prunus dulcis, cv. Tuono) in a semi-arid climate in Southern Italy. The two planting densities tested were 1660 trees per hectare (achieved with 1.5 m intra-row spacing × 4.0 m inter-row spacing) and 833 trees per hectare (3.0 m × 4.0 m spacing). The results showed that significantly lower values of annual shoot length were recorded in both 2020 and 2021, years characterized by late frosts in March and April. However, with the exception of the first year (2019), when the plants had not yet been influenced by the different planting densities, the annual shoot length was significantly higher in the lowest planting density compared to the highest one in the following years. Additionally, higher annual trunk growth values were recorded at the lower planting density compared to the higher density. By the end of the five seasons, trees at the lower density showed a cumulative trunk growth of 177 mm, whereas those at the higher density reached only 137 mm. No significant effect of the two different tree planting densities on overall fruit development, specifically length, width, and thickness, was observed. As the trees matured, kernel yield per tree increased under both planting densities. However, significantly higher individual tree yields were recorded in the lower-density configuration, reaching 2.70 kg per tree by the end of five seasons, compared to 1.68 kg per tree in the high-density arrangement. In contrast, kernel yield per hectare was greater in the densely planted configuration, achieving 2.81 t ha⁻¹, whereas the lower-density planting resulted in a yield of 2.25 t ha⁻¹ by the end of the same period. Furthermore, no significant differences were observed between the two tree planting densities in terms of the percentage of hull per fruit, kernel per nut, or the occurrence of double seeds. Similarly, morphological traits of the nuts and kernels, such as weight, length, width, and thickness, remained unaffected. However, slightly higher kernel weights were noted at the lower planting density. Full article

Climate change increases drought events, resulting in lower agricultural productivity. Almond trees (Prunus dulcis (Mill.) D.A. Webb) are drought-tolerant; however, prolonged periods of water deficit can affect plant growth and productivity. The physiological and molecular responses of two almond genotypes, Filippo Ceo and Tuono, growing in the Apulia region (Italy) under well-watered and drought conditions were evaluated. Results indicate that cv. Filippo Ceo is more drought-tolerant than cv. Tuono, exhibiting higher RWC values, good levels of photosynthetic pigments, and high proline accumulation. This behavior is confirmed by gene expression analysis, which revealed upregulation of drought-responsive genes, indicating a more effective adaptation to stress of cv. Filippo Ceo. In contrast, cv. Tuono experienced greater oxidative damage and diminished physiological response. These findings indicate that cv. Filippo Ceo could be a promising cultivar for drought-prone areas, with implications for targeted breeding and agronomic strategies to improve almond tree production under water-stressed conditions. Full article

This work aimed to propose the reuse of processing waste from the Sicilian almond (Prunus amygdalus Batsch.) cultivar Tuono for the formulation of a new functional baked product (muffin) that is gluten- and lactose-free. Muffins were prepared using orange juice, rice flour, extra virgin olive oil, and enriched almond skin (3% and 6% w/w). The chemical-physical parameters, total phenols, and flavonoids (TPC and TFC), as well as the biological properties of the ingredients and muffins, were evaluated. Sensory analyses were also conducted. DPPH, ABTS, β-carotene bleaching, and FRAP tests were applied to measure the antioxidant potential. Muffin extracts were also tested against α-amylase and α-glucosidase enzymes. Muffins enriched with 6% almond skin (M6) showed the highest TPC and TFC with values of 26.96 mg gallic acid equivalent (GAE)/g and 24.12 mg quercetin equivalent (QE)/g, respectively. M6 exerted a promising antioxidant activity as an inhibitor of lipid peroxidation, with an IC₅₀ of 15.44 μg/mL at 30 min incubation. Moreover, muffin M6 showed a promising α-glucosidase inhibitory effect (IC₅₀ of 51.82 μg/mL). Based on the obtained results and supported by sensory analysis, muffins enriched with almond skin should be proposed as a promising example of upcycling for the development of a new functional bakery product. Full article

Field experiments were conducted in three successive seasons (2019–2021) to evaluate the effects of four commercial organo-mineral fertilizers with biostimulating action (Hendophyt^®, Ergostim^®, and Radicon^®) on the vegetative and productive performance of young almond trees (Prunus dulcis, cv. Tuono) grown in a semiarid climate in Southern Italy. Foliar treatments were applied three times during each season (at the swollen bud, beginning of flowering, and fruit set-beginning of fruit growth stages). Both 2020 and 2021 were adversely affected by late frosts, resulting in damage to the flowers and small fruits without any positive effect of the biostimulant applications. In contrast, the results obtained during the normal climate year (2019) indicated that the growth of trunk diameter and shoot length of trees tended to increase in biostimulant treatments compared to those of the control. The number of buds and flowers per unit length of the branch revealed no significant differences among years and all compared treatments. However, in 2019, the fruit set percentage, number, and weight of kernels per tree were significantly higher in the biostimulant treatments compared to those of the control. To this regard, the use of biofertilizers is suitable for maintaining soil fertility and improving crop productivity This information holds significance for almond tree growers. Full article

Almonds are one of the most popular nuts, cultivated in countries with Mediterranean climates. In an almond orchard of the self-incompatible cultivar ‘Ferragnes’ in Greece, a tree with different morphological characteristics and signs of self-compatibility was observed. The aim of this study was to study the phenotype, investigate the self-compatibility trait, and elucidate the phylogenetic background of this tree, named ‘Mars’. Morphological traits and kernel and nut characteristics were measured in ‘Mars’, ‘Ferragnes’, ‘Tuono’, and ‘Lauranne’ cultivars. The self-compatibility trait of almonds is attributed to the S_f allele; thus, its existence was investigated in ‘Mars’ by PCR amplification. Moreover, the S-RNase genes of all the cultivars were sequenced. The genetic profile of ‘Mars’ was identified using eight SSR molecular markers and compared with the ‘Ferragnes’, ‘Ferraduel’, ‘Texas’, ‘Tuono’, and ‘Lauranne’ cultivars. The morphological traits suggest that ‘Mars’ is more similar to the ‘Ferragnes’ cultivar, while it bears the S_f allele. S-RNases sequencing revealed that ‘Mars’ has the genotype S₁S_f, and the SSR markers showed that it is differentiated genetic material, suggesting it is a cross between ‘Ferragnes’ and ‘Tuono’. Therefore, ‘Mars’ is evaluated as a self-compatible variety with interesting agronomic traits for use in new mono-cultivar almond plantations. Full article

The widespread use of the lung ultrasound (LUS) has not been followed by the development of a comprehensive standardized tool for its reporting in the intensive care unit (ICU) which could be useful to promote consistency and reproducibility during clinical examination. This work aims to define the essential features to be included in a standardized reporting tool and provides a structured model form to fully express the diagnostic potential of LUS and facilitate intensivists in the use of a LUS in everyday clinical ICU examination. We conducted a modified Delphi process to build consensus on the items to be integrated in a standardized report form and on its structure. A committee of 19 critical care physicians from 19 participating ICUs in Italy was formed, including intensivists experienced in ultrasound from both teaching hospitals and referral hospitals, and internationally renowned experts on the LUS. The consensus for 31 statements out of 33 was reached at the third Delphi round. A structured model form was developed based on the approved statements. The development of a standardized model as a backbone to report a LUS may facilitate the guidelines’ application in clinical practice and increase inter-operator agreement. Further studies are needed to evaluate the effects of standardized reports in critically ill patients. Full article

Maximizing water use efficiency, yield, and plant survival under drought is a relevant research issue for almond-tree-growing areas worldwide. The intraspecific diversity of this species may constitute a valuable resource to address the resilience and productivity challenges that climate change poses to crop sustainability. A comparative evaluation of physiological and productive performance of four almond varieties: ‘Arrubia’, ‘Cossu’, ‘Texas’, and ‘Tuono’, field-grown in Sardinia, Italy, was performed. A great variability in the plasticity to cope with soil water scarcity and a diverse capacity to adapt to drought and heat stresses during fruit development were highlighted. The two Sardinian varieties, Arrubia and Cossu, showed differences in water stress tolerance, photosynthetic and photochemical activity, and crop yield. ‘Arrubia’ and ‘Texas’ showed greater physiological acclimation to water stress while maintaining higher yields, as compared to the self-fertile ‘Tuono’. The important role of crop load and specific anatomical traits affecting leaf hydraulic conductance and leaf gas exchanges efficiency (i.e., dominant shoot type, leaf size and roughness) was evidenced. The study highlights the importance of characterizing the relationships among almond cultivar traits that affect plant performance under drought in order to better assist planting choices and orchard irrigation management for given environmental contexts. Full article

In this study we developed a new extract, by the use of conventional solid-solvent extraction and a food-grade hydroalcoholic solvent, rich in phenolic and triterpenoid components from almon hull to be employed as functional ingredient in food, pharma and cosmetic sectors. Two autochthonous Sicilian cultivars (‘Pizzuta’ and ‘Romana’) and an Apulian modern cultivar (‘Tuono’) have been tested for the production of the extract. Results showed that the two Sicilian varieties, and in particular the ‘Romana’ one, present the best characteristics to obtain extracts rich in triterpenoids and hydroxycinnamic acids, useful for the production of nutraceutical supplements. About triterpenoids, the performance of the hydroalcoholic extraction process allowed to never go below 46% of recovery for ‘Pizzuta’ samples, with significantly higher percentages of recovery for ‘Tuono’ and ‘Romana’ extracts (62.61% and 73.13%, respectively) while hydroxycinnamic acids were recovered at higher recovery rate (84%, 89% and 88% for ‘Pizzuta’, ‘Romana’ and ‘Tuono’ extracts, respectively). Invitro antioxidant and antimicrobial activities exerted by the extracts showed promising results with P. aeruginosa being the most affected strain, inhibited up to the 1/8 dilution with ‘Romana’ extract. All the three tested extracts exerted an antimicrobial action up to 1/4 dilutions but ‘Romana’ and ‘Pizzuta’ extracts always showed the greatest efficacy. Full article

Lung ultrasound (LUS) is a validated technique for the prompt diagnosis and bedside monitoring of critically ill patients due to its availability, safety profile, and cost-effectiveness. The aim of this work is to detect similarities and differences among LUS reports performed in ICUs and to provide a common ground for an integrated report form. We collected all LUS reports during an index week in 21 ICUs from the GiViTI network. First, we considered signs, chest areas, and terminology reported. Then, we compared different report structures and categorized them as structured reports (SRs), provided with a predefined model form, and free unstructured text reports (FTRs) that had no predetermined structure. We analyzed 171 reports from 21 ICUs, and 59 reports from 5 ICUs were structured. All the reports presented a qualitative description that mainly focused on the presence of B-lines, consolidations, and pleural effusion. Zones were defined in 66 reports (39%). In SRs, a complete examination of all the regions was more frequently achieved (96% vs. 74%), and a higher impact on therapeutic strategies was observed (17% vs. 6%). LUS reports vary significantly among different centers. Adopting an integrated SR seems to promote a systematic approach in scanning and reporting, with a potential impact on LUS clinical applications. Full article

The almond [Prunus dulcis (Mill.) D.A. Webb] is normally self-sterile, requiring orchard placement of pollinizer cultivars and insect pollinators. Honeybees are the primary insect pollinators utilized, but climate change and the higher frequency of extreme weather events have reduced their availability to levels insufficient to meet the demands of current and anticipated almond acreage. The incorporation of self-fruitfulness may eliminate the need for both pollinizers and pollinators and allow the planting of single cultivar orchards that facilitate orchard management and reduce agrochemical inputs. Self-fruitfulness requires self-compatibility of self-pollen tube growth to fertilization, as well as a high level of consistent self-pollination or autogamy over the range of anticipated bloom environments. The Italian cultivar Tuono has been the sole source of self-compatibility for breeding programs world-wide, leading to high levels of inbreeding in current almond improvement programs. Both self-compatibility and autogamy have been successfully transferred to commercial almonds from cultivated peaches (Prunus persica L.), as well as wild peach and almond species. Self-compatibility was inherited as a novel major gene, but was also influenced by modifiers. Molecular markers developed for one species source often failed to function for other species’ sources. Autogamy was inherited as a quantitative trait. Breeding barriers were more severe in the early stages of trait introgression, but rapidly diminished by the second to third backcross. Increasing kernel size, which was similarly inherited as a quantitative trait, was a major regulator of the introgression rate. Self-fruitfulness, along with good commercial performance of tree and nut traits, was recovered from different species sources, including Prunus mira, Prunus webbii, P. persica, and the P. webbii-derived Italian cultivar Tuono. Differences in expression of self-fruitfulness were observed, particularly during field selection at the early growth stages. Introgression of self-fruitfulness from these diverse sources also enriched overall breeding germplasm, allowing the introduction of useful traits that are not accessible within traditional germplasm. Full article

In the new introducing almond areas, it is necessary to test the more promising almond cultivar and rootstock combinations able to guarantee the best agronomic performances according to the specific pedoclimatic conditions. With this aim, two almond trials have been established in an experimental farm located in the Latium region (Italy). The first trial (A) focused on the phenological, and agronomical influences induced by the clonal rootstock ‘GF677’ on the grafted cultivars ‘Tuono’, ‘Supernova’ and ‘Genco’, in comparison to those induced by peach seedling rootstocks, in order to identify the best grafting combination for developing “high density” plantings in this new growing area. The second trial (B) tested the phenological and agronomical influences induced by three different clonal rootstocks (‘GF677’, ‘Rootpac^® 20’ and ‘Rootpac^® R’), on the Spanish cultivar ‘Guara’ to identify suitable dwarfing rootstocks for “super high density” plantings in the same environment. Flowering and ripening calendars of the trial A highlighted as the medium-late flowering cultivars ‘Genco’, ‘Supernova’ and ‘Tuono’ could be subject to moderate risk of cold damages. The clonal rootstock ‘GF677’ seems to anticipate flowering and vegetative bud break by a few days in ‘Tuono’ when compared to the same cultivar grafted on peach seedling rootstocks. Furthermore, the yield per plant was always higher in plants grafted on ‘GF677’. The observations carried out in trial B highlighted as the flowering of cultivar ‘Guara’ were affected by the rootstock, with ‘Rootpac^® 20’, which postponed its full bloom of about one week when compared to other rootstocks, whereas ‘GF677’ imposed more vigor to the cultivar than ‘Rootpac^® 20’ and ‘Rootpac^® R’. Full article

Modern almond growing travels on the tracks of super-high density (SHD). Born in 2010, it has already reached 6700 ha planted all over the world. This new cultivation system needs to define efficient agronomic techniques in order to identify it as a “Super-Efficient System”. Among these, the choice of cultivar is a crucial technique and a key factor for sustainability. The purpose of this study was to compare different cultivars in terms of vegetative, productive, and efficiencies parameters in order to gain applicable relevant knowledge about the SHD almond cultivation technique. For this, 3 years of research was carried out during 2017–2019, on a young almond grove made in 2014 with row spacing of 3.80 m × 1.20 m (2190 trees/ha), to evaluate the agronomic behavior of the two most planted cultivars in Italy, Guara-Tuono and Lauranne^® Avijor, grafted on the Rootpac^®20 dwarfing rootstock. The main biometric, productive, yield, mechanical harvesting efficiencies, and almond quality parameters were evaluated. Cv Lauranne^® showed greater vigor, greater fruit yield, and damaged axes by mechanical harvesting, while higher values of yield efficiencies were observed for cv Tuono. Harvesting efficiency was related to canopy size and tree age. On the contrary, almonds quality parameters were strongly related to the cultivar, confirming the good performance of Tuono as varietal characters. Then, this cultivar seems to be the most suitable for an efficient SHD planting system, in line with the objectives of modern sustainable fruit growing. The better performance of cv Tuono could be related to the positive influence of the terroir as well. Full article

In the present study, the influence of almond variety on color, chemical, physical and sensory characteristics of “amaretti” cookies during the shelf life, was assessed. Four varieties were chosen for the study, two of which were local (Cossu, Arrubia) and two widely cultivated (Tuono, Texas). Almonds have been characterized in the content of proteins, crude fat, amygdalin and fatty acids profile. The evolution of the characteristics during the shelf life hasbeen measured through image data modeling, texture, physical chemical and sensory analyses. Data were then treated with a multivariate approach performing a PCA. Image analysis and fitting on log normal and powerlaw functions highlighted the influence of the variety on the total area affected by surface breakages, and on the distribution of the cracking surfaces dimension classes. Texture parameters (crust hardness, thickness and work of deformation) were negatively correlated to moisture content. Sensory profile confirmed the differences in tactile features measured through instrumental texture, while slight to no differences were found in odor profile. Consumer test showed an higher acceptability for Arrubia, Texas and Tuono samples throughout the shelf life, while Cossu samples were less accepted. Overall, the choice of almond variety influences product features and liking of almond products, therefore it represents an important phase to direct the choice of both farmers and confectionery manufacturers. Full article