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29 pages, 12050 KiB  
Article
PolSAR-SFCGN: An End-to-End PolSAR Superpixel Fully Convolutional Generation Network
by Mengxuan Zhang, Jingyuan Shi, Long Liu, Wenbo Zhang, Jie Feng, Jin Zhu and Boce Chu
Remote Sens. 2025, 17(15), 2723; https://doi.org/10.3390/rs17152723 - 6 Aug 2025
Abstract
Polarimetric Synthetic Aperture Radar (PolSAR) image classification is one of the most important applications in remote sensing. The impressive superpixel generation approaches can improve the efficiency of the subsequent classification task and restrain the influence of the speckle noise to an extent. Most [...] Read more.
Polarimetric Synthetic Aperture Radar (PolSAR) image classification is one of the most important applications in remote sensing. The impressive superpixel generation approaches can improve the efficiency of the subsequent classification task and restrain the influence of the speckle noise to an extent. Most of the classical PolSAR superpixel generation approaches use the features extracted manually and even only consider the pseudocolor images. They do not make full use of polarimetric information and do not necessarily lead to good enough superpixels. The deep learning methods can extract effective deep features but they are difficult to combine with superpixel generation to achieve true end-to-end training. Addressing the above issues, this study proposes an end-to-end fully convolutional superpixel generation network for PolSAR images. It integrates the extraction of polarization information features and the generation of PolSAR superpixels into one step. PolSAR superpixels can be generated based on deep polarization feature extraction and need no traditional clustering process. Both the performance and efficiency of generations of PolSAR superpixels can be enhanced effectively. The experimental results on various PolSAR datasets show that the proposed method can achieve impressive superpixel segmentation by fitting the real boundaries of different types of ground objects effectively and efficiently. It can achieve excellent classification performance by connecting a very simple classification network, which is helpful to improve the efficiency of the subsequent PolSAR image classification tasks. Full article
18 pages, 4216 KiB  
Article
Screening and Application of Highly Efficient Rhizobia for Leguminous Green Manure Astragalus sinicus in Lyophilized Inoculants and Seed Coating
by Ding-Yuan Xue, Wen-Feng Chen, Guo-Ping Yang, You-Guo Li and Jun-Jie Zhang
Plants 2025, 14(15), 2431; https://doi.org/10.3390/plants14152431 - 6 Aug 2025
Abstract
Astragalus sinicus, a key leguminous green manure widely cultivated in Southern China’s rice-based cropping systems, plays a pivotal role in sustainable agriculture by enhancing soil organic matter sequestration, improving rice yield, and elevating grain quality. The symbiotic nitrogen-fixing association between A. sinicus [...] Read more.
Astragalus sinicus, a key leguminous green manure widely cultivated in Southern China’s rice-based cropping systems, plays a pivotal role in sustainable agriculture by enhancing soil organic matter sequestration, improving rice yield, and elevating grain quality. The symbiotic nitrogen-fixing association between A. sinicus and its matching rhizobia is fundamental to its agronomic value; however, suboptimal inoculant efficiency and field application methodologies constrain its full potential. To address these limitations, we conducted a multi-phase study involving (1) rhizobial strain screening under controlled greenhouse conditions, (2) an optimized lyophilization protocol evaluating cryoprotectant (trehalose, skimmed milk powder and others), and (3) seed pelleting trails with rhizobial viability and nodulation assessments over different storage periods. Our results demonstrate that Mesorhizobium huakuii CCBAU 33470 exhibits a superior nitrogen-fixing efficacy, significantly enhancing key traits in A. sinicus, including leaf chlorophyll content, tiller number, and aboveground biomass. Lyophilized inoculants prepared with cryoprotectants (20% trehalose or 20% skimmed milk powder) maintained >90% bacterial viability for 60 days and markedly improved nodulation capacity relative to unprotected formulations. The optimized seed pellets sustained high rhizobial loads (5.5 × 103 cells/seed) with an undiminished viability after 15 days of storage and nodulation ability after 40 days of storage. This integrated approach of rhizobial selection, inoculant formulation, and seed coating overcomes cultivation bottlenecks, boosting symbiotic nitrogen fixation for A. sinicus cultivation. Full article
(This article belongs to the Topic New Challenges on Plant–Microbe Interactions)
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15 pages, 1806 KiB  
Article
Drought and Shrub Encroachment Accelerate Peatland Carbon Loss Under Climate Warming
by Fan Lu, Boli Yi, Jun-Xiao Ma, Si-Nan Wang, Yu-Jie Feng, Kai Qin, Qiansi Tu and Zhao-Jun Bu
Plants 2025, 14(15), 2387; https://doi.org/10.3390/plants14152387 - 2 Aug 2025
Viewed by 185
Abstract
Peatlands store substantial amounts of carbon (C) in the form of peat, but are increasingly threatened by drought and shrub encroachment under climate warming. However, how peat decomposition and its temperature sensitivity (Q10) vary with depth and plant litter input [...] Read more.
Peatlands store substantial amounts of carbon (C) in the form of peat, but are increasingly threatened by drought and shrub encroachment under climate warming. However, how peat decomposition and its temperature sensitivity (Q10) vary with depth and plant litter input under these stressors remains poorly understood. We incubated peat from two depths with different degrees of decomposition, either alone or incubated with Sphagnum divinum shoots or Betula ovalifolia leaves, under five temperature levels and two moisture conditions in growth chambers. We found that drought and Betula addition increased CO2 emissions in both peat layers, while Sphagnum affected only shallow peat. Deep peat alone or with Betula exhibited higher Q10 than pure shallow peat. Drought increased the Q10 of both depths’ peat, but this effect disappeared with fresh litter addition. The CO2 production rate showed a positive but marginal correlation with microbial biomass carbon, and it displayed a rather similar responsive trend to warming as the microbial metabolism quotient. These results indicate that both deep and dry peat are more sensitive to warming, highlighting the importance of keeping deep peat buried and waterlogged to conserve existing carbon storage. Additionally, they further emphasize the necessity of Sphagnum moss recovery following vascular plant encroachment in restoring carbon sink function in peatlands. Full article
(This article belongs to the Section Plant–Soil Interactions)
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13 pages, 1594 KiB  
Article
Unraveling Nitrogen Removal and Microbial Response of Integrated Sulfur-Driven Partial Denitrification and Anammox Process in Saline Wastewater Treatment
by Xiangchen Li, Jie Sun, Zonglun Cao, Junxi Lai, Haodi Feng and Minwen Guo
Water 2025, 17(15), 2284; https://doi.org/10.3390/w17152284 - 31 Jul 2025
Viewed by 285
Abstract
Increasing the discharge of saline wastewater from an industrial field poses a challenge for applicable Anammox-based technologies. This study established the integrated partial sulfur-driven denitrification and Anammox (SPDA) system to explore the effects of different salinity levels on nitrogen conversion features. The results [...] Read more.
Increasing the discharge of saline wastewater from an industrial field poses a challenge for applicable Anammox-based technologies. This study established the integrated partial sulfur-driven denitrification and Anammox (SPDA) system to explore the effects of different salinity levels on nitrogen conversion features. The results of batch tests suggested that sulfur-driven denitrification exhibited progressive suppression of nitrate reduction (97.7% → 12.3% efficiency at 0% → 4% salinity) and significant nitrite accumulation (56.4% accumulation rate at 2% salinity). Anammox showed higher salinity tolerance but still experienced drastic TN removal decline (97.6% → 17.3% at 0% → 4% salinity). Long-term operation demonstrated that the SPDA process could be rapidly established at 0% salinity and stabilize with TN removal efficiencies of 98.1% (1% salinity), 72.8% (2% salinity), and 70.2% (4% salinity). The robustness of the system was attributed to the appropriate strategy of gradual salinity elevation, the promoted secretion of protein-dominated EPS, the salinity-responsive enrichment of Sulfurimonas (replacing Thiobacillus and Ferritrophicum) as sulfur-oxidizing bacteria (SOB), and the sustained retention and activity of Brocadia as AnAOB. The findings in this study deepen the understanding of the inhibitory effects of salinity on the SPDA system, providing a feasible solution for saline wastewater treatment with low cost and high efficiency. Full article
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19 pages, 2954 KiB  
Article
Static Analysis of Temperature-Dependent FGM Spherical Shells Under Thermo-Mechanical Loads
by Zhong Zhang, Zhiting Feng, Zhan Shi, Honglei Xie, Ying Sun, Zhenyuan Gu, Jie Xiao and Jiajing Xu
Buildings 2025, 15(15), 2709; https://doi.org/10.3390/buildings15152709 - 31 Jul 2025
Viewed by 99
Abstract
Static analysis is conducted for functionally graded material (FGM) spherical shells under thermo-mechanical loads, based on the three-dimensional thermo-elasticity theory. The material properties, which vary with both the radial coordinate and temperature, introduce nonlinearity to the problem. To address this, a layer model [...] Read more.
Static analysis is conducted for functionally graded material (FGM) spherical shells under thermo-mechanical loads, based on the three-dimensional thermo-elasticity theory. The material properties, which vary with both the radial coordinate and temperature, introduce nonlinearity to the problem. To address this, a layer model is proposed, wherein the shell is discretized into numerous concentric spherical layers, each possessing uniform material properties. Within this framework, the nonlinear heat conduction equations are first solved iteratively. The resulting temperature field is then applied to the thermo-elastic equations, which are subsequently solved using a combined state space and transfer matrix method to obtain displacement and stress solutions. Comparison with existing literature results demonstrates good agreement. Finally, a parametric study is presented to investigate the effects of material temperature dependence and gradient index on the thermo-mechanical behaviors of the FGM spherical shells. Full article
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18 pages, 8192 KiB  
Article
Microstructure, Mechanical Properties, and Tribological Behavior of Friction Stir Lap-Welded Joints Between SiCp/Al–Fe–V–Si Composites and an Al–Si Alloy
by Shunfa Xiao, Pinming Feng, Xiangping Li, Yishan Sun, Haiyang Liu, Jie Teng and Fulin Jiang
Materials 2025, 18(15), 3589; https://doi.org/10.3390/ma18153589 - 30 Jul 2025
Viewed by 267
Abstract
Aluminum matrix composites provide an ideal solution for lightweight brake disks, but conventional casting processes are prone to crack initiation due to inhomogeneous reinforcement dispersion, gas porosity, and inadequate toughness. To break the conventional trade-off between high wear resistance and low toughness of [...] Read more.
Aluminum matrix composites provide an ideal solution for lightweight brake disks, but conventional casting processes are prone to crack initiation due to inhomogeneous reinforcement dispersion, gas porosity, and inadequate toughness. To break the conventional trade-off between high wear resistance and low toughness of brake disks, this study fabricated a bimetallic structure of SiCp/Al–Fe–V–Si aluminum matrix composite and cast ZL101 alloy using friction stir lap welding (FSLW). Then, the microstructural evolution, mechanical properties, and tribological behavior of the FSLW joints were studied by XRD, SEM, TEM, tensile testing, and tribological tests. The results showed that the FSLW process homogenized the distribution of SiC particle reinforcements in the SiCp/Al–Fe–V–Si composites. The Al12(Fe,V)3Si heat-resistant phase was not decomposed or coarsened, and the mechanical properties were maintained. The FSLW process refined the grains of the ZL101 aluminum alloy through recrystallization and fragmented eutectic silicon, improving elongation to 22%. A metallurgical bond formed at the joint interface. Tensile fracture occurred within the ZL101 matrix, demonstrating that the interfacial bond strength exceeded the alloy’s load-bearing capacity. In addition, the composites exhibited significantly enhanced wear resistance after FSLW, with their wear rate reduced by approximately 40% compared to the as-received materials, which was attributed to the homogenized SiC particle distribution and the activation of an oxidative wear mechanism. Full article
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18 pages, 6795 KiB  
Article
Strain-Rate-Dependent Tensile Behaviour and Viscoelastic Modelling of Kevlar® 29 Plain-Woven Fabric for Ballistic Applications
by Kun Liu, Ying Feng, Bao Kang, Jie Song, Zhongxin Li, Zhilin Wu and Wei Zhang
Polymers 2025, 17(15), 2097; https://doi.org/10.3390/polym17152097 - 30 Jul 2025
Viewed by 190
Abstract
Aramid fibre has become a critical material for individual soft body armour due to its lightweight nature and exceptional impact resistance. To investigate its energy absorption mechanism, quasi-static and dynamic tensile experiments were conducted on Kevlar® 29 plain-woven fabric using a universal [...] Read more.
Aramid fibre has become a critical material for individual soft body armour due to its lightweight nature and exceptional impact resistance. To investigate its energy absorption mechanism, quasi-static and dynamic tensile experiments were conducted on Kevlar® 29 plain-woven fabric using a universal material testing machine and a Split Hopkinson Tensile Bar (SHTB) apparatus. Tensile mechanical responses were obtained under various strain rates. Fracture morphology was characterised using scanning electron microscopy (SEM) and ultra-depth three-dimensional microscopy, followed by an analysis of microstructural damage patterns. Considering the strain rate effect, a viscoelastic constitutive model was developed. The results indicate that the tensile mechanical properties of Kevlar® 29 plain-woven fabric are strain-rate dependent. Tensile strength, elastic modulus, and toughness increase with strain rate, whereas fracture strain decreases. Under quasi-static loading, the fracture surface exhibits plastic flow, with slight axial splitting and tapered fibre ends, indicating ductile failure. In contrast, dynamic loading leads to pronounced axial splitting with reduced split depth, simultaneous rupture of fibre skin and core layers, and fibrillation phenomena, suggesting brittle fracture characteristics. The modified three-element viscoelastic constitutive model effectively captures the strain-rate effect and accurately describes the tensile behaviour of the plain-woven fabric across different strain rates. These findings provide valuable data support for research on ballistic mechanisms and the performance optimisation of protective materials. Full article
(This article belongs to the Section Polymer Composites and Nanocomposites)
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19 pages, 13331 KiB  
Article
Multi-Scale Study on Ultrasonic Cutting of Nomex Honeycomb Composites of Disc Cutters
by Yiying Liang, Feng Feng, Wenjun Cao, Ge Song, Xinman Yuan, Jie Xu, Qizhong Yue, Si Pan, Enlai Jiang, Yuan Ma and Pingfa Feng
Materials 2025, 18(15), 3476; https://doi.org/10.3390/ma18153476 - 24 Jul 2025
Viewed by 208
Abstract
To address the issues of burr formation, structural deformation, and tearing in the conventional machining of Nomex honeycomb composites, this study aims to clarify the mechanisms by which ultrasonic vibration-assisted cutting enhances machining quality. A multi-scale analysis framework is developed to examine the [...] Read more.
To address the issues of burr formation, structural deformation, and tearing in the conventional machining of Nomex honeycomb composites, this study aims to clarify the mechanisms by which ultrasonic vibration-assisted cutting enhances machining quality. A multi-scale analysis framework is developed to examine the effects of ultrasonic vibration on fiber distribution, cell-level shear response, and the overall cutting mechanics. At the microscale, analyses show that ultrasonic vibration mitigates stress concentrations, thereby shortening fiber length. At the mesoscale, elastic buckling and plastic yielding models show that ultrasonic vibration lowers shear strength and modifies the deformation. A macro-scale comparison of cutting behavior with and without ultrasonic vibration was conducted. The results indicate that the intermittent contact effect induced by vibration significantly reduces cutting force. Specifically, at an amplitude of 40 μm, the cutting force decreased by approximately 29.7% compared to the condition without ultrasonic vibration, with an average prediction error below 8.6%. Compared to conventional machining, which causes the honeycomb angle to deform to approximately 130°, ultrasonic vibration preserves the original 120° geometry and reduces burr length by 36%. These results demonstrate that ultrasonic vibration effectively reduces damage through multi-scale interactions, offering theoretical guidance for high-precision machining of fiber-reinforced composites. Full article
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17 pages, 3179 KiB  
Article
Changes in Physical Parameters of CO2 Containing Impurities in the Exhaust Gas of the Purification Plant and Selection of Equations of State
by Xinyi Wang, Zhixiang Dai, Feng Wang, Qin Bie, Wendi Fu, Congxin Shan, Sijia Zheng and Jie Sun
Fluids 2025, 10(8), 189; https://doi.org/10.3390/fluids10080189 - 23 Jul 2025
Viewed by 267
Abstract
CO2 transport is a crucial part of CCUS. Nonetheless, due to the physical property differences between CO2 and natural gas and oil, CO2 pipeline transport is distinct from natural gas and oil transport. Gaseous CO2 transportation has become the [...] Read more.
CO2 transport is a crucial part of CCUS. Nonetheless, due to the physical property differences between CO2 and natural gas and oil, CO2 pipeline transport is distinct from natural gas and oil transport. Gaseous CO2 transportation has become the preferred scheme for transporting impurity-containing CO2 tail gas in purification plants due to its advantages of simple technology, low cost, and high safety, which are well suited to the scenarios of low transportation volume and short distance in purification plants. The research on its physical property and state parameters is precisely aimed at optimizing the process design of gaseous transportation so as to further improve transportation efficiency and safety. Therefore, it has important engineering practical significance. Firstly, this paper collected and analyzed the research cases of CO2 transport both domestically and internationally, revealing that phase state and physical property testing of CO2 gas containing impurities is the basic condition for studying CO2 transport. Subsequently, the exhaust gas captured by the purification plant was captured after hydrodesulfurization treatment, and the characteristics of the exhaust gas components were obtained by comparing before and after treatment. By preparing fluid samples with varied CO2 content and conducting the flash evaporation test and PV relationship test, the compression factor and density of natural gas under different temperatures and pressures were obtained. It is concluded that under the same pressure in general, the higher the CO2 content, the smaller the compression factor. Except for pure CO2, the higher the CO2 content, the higher the density under constant pressure, which is related to the content of C2 and heavier hydrocarbon components. At the same temperature, the higher the CO2 content, the higher the viscosity under the same pressure; the lower the pressure, the slower the viscosity growth slows down. The higher the CO2 content at the same temperature, the higher the specific heat at constant pressure. With the decrease in temperature, the CO2 content reaching the highest specific heat at the identical pressure gradually decreases. Finally, BWRS, PR, and SRK equations of state were utilized to calculate the compression factor and density of the gas mixture with a molar composition of 50% CO2 and the gas mixture with a molar composition of 100% CO2. Compared with the experimental results, the most suitable equation of state is selected as the PR equation, which refers to the parameter setting of critical nodes of CO2 gas transport. Full article
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18 pages, 2407 KiB  
Article
IFDA: Intermittent Fault Diagnosis Algorithm for Augmented Cubes Under the PMC Model
by Chongwen Yuan, Chenghao Zou, Jiong Wu, Hao Feng and Jie Li
Appl. Sci. 2025, 15(15), 8197; https://doi.org/10.3390/app15158197 - 23 Jul 2025
Viewed by 149
Abstract
Fault diagnosis technology is a crucial technique for ensuring the reliability of multiprocessor systems. Many previous studies have paid close attention to the permanent faults of systems while ignoring the rise of intermittent faults. Meanwhile, there is a lack of a rapid diagnostic [...] Read more.
Fault diagnosis technology is a crucial technique for ensuring the reliability of multiprocessor systems. Many previous studies have paid close attention to the permanent faults of systems while ignoring the rise of intermittent faults. Meanwhile, there is a lack of a rapid diagnostic algorithm tailored for intermittent faults. In this paper, we propose multiple theorems to evaluate the intermittent fault diagnosability of different topologies under the PMC model. Through these theorems, we demonstrate that the intermittent fault diagnosability of an n-dimensional augmented cube (AQn) is (2n2) when n is greater than or equal to 4. Furthermore, we present a fast intermittent fault diagnosis algorithm, which is named as IFDA, to identify the processors with intermittent fault in the networks. Finally, we evaluate the performance of the algorithm in terms of the parameters Accuracy and Precision. The simulation experimental results show that the algorithm IFDA has good performance and efficiency. Full article
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16 pages, 3034 KiB  
Article
Identification of Avocado Fruit Disease Caused by Diaporthe phaseolorum and Colletotrichum fructicola in China
by Aosiqi Ma, Yuhang Xu, Hongxing Feng, Yanyuan Du, Huan Liu, Song Yang, Jie Chen and Xin Hao
J. Fungi 2025, 11(8), 547; https://doi.org/10.3390/jof11080547 - 23 Jul 2025
Viewed by 454
Abstract
Persea americana (avocado) is a healthy fruit, rich in unsaturated fatty acids, various minerals, and vitamins. As avocado cultivation continues to expand globally, its development is increasingly constrained by concomitant diseases, among which fruit rot and anthracnose have emerged as significant threats to [...] Read more.
Persea americana (avocado) is a healthy fruit, rich in unsaturated fatty acids, various minerals, and vitamins. As avocado cultivation continues to expand globally, its development is increasingly constrained by concomitant diseases, among which fruit rot and anthracnose have emerged as significant threats to fruit quality. Menglian in Yunnan Province is the largest avocado production area in China. In November 2024, fruit rot was observed on avocado fruits in Yunnan, China, characterized by reddish-brown discoloration, premature ripening, softening, and pericarp decay, with a field infection rate of 22%. Concurrently, anthracnose was detected in avocado fruits, presenting as small dark brown spots that developed into irregular rust-colored lesions, followed by dry rot depressions, ultimately leading to soft rot, peeling, or hardened dry rot, with a field infection rate of 15%. Infected fruit samples were collected, and fungal strains were isolated, purified, and inoculated via spore suspension, followed by re-isolation. The strains were conclusively identified as Diaporthe phaseolorum (SWFU20, SWFU21) and Colletotrichum fructicola (SWFU12, SWFU13) through an integrated approach combining DNA extraction, polymerase chain reaction (PCR), sequencing, phylogenetic reconstruction, and morphological characterization. This is the first report of D. phaseolorum causing fruit rot and C. fructicola causing anthracnose on avocado in China. In future research, we will test methods for the control of D. phaseolorum and C. fructicola. The identification of these pathogens provides a foundation for future disease management research, supporting the sustainable development of the avocado industry. Full article
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22 pages, 4544 KiB  
Article
Aspirin Eugenol Ester Ameliorates HFD-Induced NAFLD in Mice via the Modulation of Bile Acid Metabolism
by Zhi-Jie Zhang, Qi Tao, Ji Feng, Qin-Fang Yu, Li-Ping Fan, Zi-Hao Wang, Wen-Bo Ge, Jian-Yong Li and Ya-Jun Yang
Int. J. Mol. Sci. 2025, 26(15), 7044; https://doi.org/10.3390/ijms26157044 - 22 Jul 2025
Viewed by 197
Abstract
Non-alcoholic fatty liver disease (NAFLD) is a highly prevalent condition worldwide and represents a major global health challenge. Pharmacological and pharmacodynamic results indicate that aspirin eugenol ester (AEE) performs various pharmacological activities. However, it is unclear whether AEE can ameliorate the NAFLD. This [...] Read more.
Non-alcoholic fatty liver disease (NAFLD) is a highly prevalent condition worldwide and represents a major global health challenge. Pharmacological and pharmacodynamic results indicate that aspirin eugenol ester (AEE) performs various pharmacological activities. However, it is unclear whether AEE can ameliorate the NAFLD. This study investigated the ameliorative effects of AEE on glucose and lipid metabolism disorders by in vitro and in vivo experiments. In the cellular model, TC increased to 0.104 μmol/mg and TG increased to 0.152 μmol/mg in the model group, while TC decreased to 0.043 μmol/mg and TG decreased to 0.058 μmol/mg in the AEE group. In the model group, the area occupied by lipid droplets within the visual field was significantly elevated to 17.338%. However, the administration of AEE resulted in a substantial reduction in this area to 10.064%. AEE significantly reduced the lipid droplet area and TC and TG levels (p < 0.05), increased bile acids in the cells and in the medium supernatant (p < 0.05), and significantly up-regulated the expression of LRH-1, PPARα, CYP7A1, and BSEP mRNA levels (p < 0.05) compared to the model group. In the animal model, different doses of AEE administration significantly down-regulated the levels of TC, TG, LDL, GSP, and FBG (p < 0.05) compared to the high-fat-diet (HFD) group, and 216 mg/kg of AEE significantly improved hepatocellular steatosis, attenuated liver injury, and reduced the area of glycogen staining (p < 0.05). In the HFD group, the glycogen area within the visual field exhibited a significant increase to 18.250%. However, the administration of AEE resulted in a notable reduction in the glycogen area to 13.314%. Liver and serum metabolomics results show that AEE can reverse the metabolite changes caused by a HFD. The major metabolites were involved in seven pathways, including riboflavin metabolism, glycerophospholipid metabolism, tryptophan metabolism, primary bile acid biosynthesis, biosynthesis of unsaturated fatty acids, nicotinate and nicotinamide metabolism, and tryptophan metabolism. In conclusion, AEE had a positive regulatory effect on NAFLD. Full article
(This article belongs to the Special Issue Using Model Organisms to Study Complex Human Diseases)
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15 pages, 5560 KiB  
Article
Integrated Transcriptomic Analysis Reveals Molecular Mechanisms Underlying Albinism in Schima superba Seedlings
by Jie Jia, Mengdi Chen, Yuanheng Feng, Zhangqi Yang and Peidong Yan
Forests 2025, 16(8), 1201; https://doi.org/10.3390/f16081201 - 22 Jul 2025
Viewed by 253
Abstract
The main objective of this study was to reveal the molecular mechanism of the albinism in Schima superba and to identify the related functional genes to provide theoretical support for the optimization of S. superba seedling nursery technology. Combining third-generation SMRT sequencing with [...] Read more.
The main objective of this study was to reveal the molecular mechanism of the albinism in Schima superba and to identify the related functional genes to provide theoretical support for the optimization of S. superba seedling nursery technology. Combining third-generation SMRT sequencing with second-generation high-throughput sequencing technology, the transcriptomes of normal seedlings and albinism seedlings of S. superba were analyzed and the sequencing data were functionally annotated and deeply resolved. The results showed that 270 differentially expressed transcripts were screened by analyzing second-generation sequencing data. KEGG enrichment analysis of the annotation information revealed that, among the photosynthesis-antenna protein-related pathways, the expression of LHCA3 and LHCB6 was found to be down-regulated in S. superba albinism seedlings, suggesting that the down-regulation of photosynthesis-related proteins may affect the development of chloroplasts in leaves. Down-regulated expression of VDE in the carotenoid biosynthesis leads to impaired chlorophyll cycling. In addition, transcription factors (TFs), such as bHLH, MYB, GLK and NAC, were closely associated with chloroplast development in S. superba seedlings. In summary, the present study systematically explored the transcriptomic features of S. superba albinism seedlings, screened out key genes with significant differential expression and provide a reference for further localization and cloning of the key genes for S. superba albinism, in addition to laying an essential theoretical foundation for an in-depth understanding of the molecular mechanism of the S. superba albinism. The genes identified in this study that are associated with S. superba albinism will be important targets for genetic modification or molecular marker development, which is essential for improving the cultivation efficiency of S. superba. Full article
(This article belongs to the Special Issue Forest Tree Breeding: Genomics and Molecular Biology)
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19 pages, 2239 KiB  
Article
Experimental Study on Mechanical Differences Between Prefabricated and Cast-In Situ Tunnel Linings Based on a Load-Structure Model
by Li-Ming Wu, Hong-Kun Li, Feng Gao, Zi-Jian Wang, Bin Zhang, Wen-Jie Luo and Jun-Jie Li
Buildings 2025, 15(14), 2522; https://doi.org/10.3390/buildings15142522 - 18 Jul 2025
Viewed by 273
Abstract
With the accelerated development of urban underground spaces, prefabricated tunnel linings have become a research focus due to their advantages in construction efficiency and cost effectiveness. However, issues such as stress concentration at joints and insufficient overall stability hinder their broader application. This [...] Read more.
With the accelerated development of urban underground spaces, prefabricated tunnel linings have become a research focus due to their advantages in construction efficiency and cost effectiveness. However, issues such as stress concentration at joints and insufficient overall stability hinder their broader application. This study investigates a cut-and-cover prefabricated tunnel project in the Chongqing High-Tech Zone through scale model tests and numerical simulations to systematically compare the mechanical behaviors of cast-in situ linings and three-segment prefabricated linings under surrounding rock loads. The experimental results show that the ultimate bearing capacity of the prefabricated lining is 15.3% lower than that of the cast-in situ lining, with asymmetric failure modes and cracks concentrated near joint regions. Numerical simulations further reveal the influence of joint stiffness on structural performance: when the joint stiffness is 30 MN·m/rad, the bending moment of the segmented lining decreases by 37.7% compared to the cast-in situ lining, while displacement increments remain controllable. By optimising joint pre-tightening forces and stiffness parameters, prefabricated linings can achieve stability comparable to cast-in situ structures while retaining construction efficiency. This research provides theoretical and technical references for the design and construction of open-cut prefabricated tunnel linings. Full article
(This article belongs to the Section Building Structures)
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15 pages, 827 KiB  
Article
Evolution of Resistant Mutants in Pseudomonas aeruginosa Persister Cells Under Meropenem Treatment
by Jie Feng, Yifan Bian, Congjuan Xu, Zhihui Cheng, Yongxin Jin, Shouguang Jin and Weihui Wu
Microorganisms 2025, 13(7), 1672; https://doi.org/10.3390/microorganisms13071672 - 16 Jul 2025
Viewed by 275
Abstract
Bacterial persisters are dormant cells that survive antibiotic treatment, serving as a reservoir for the emergence of resistant mutations. The evolution of antibiotic resistance poses a significant challenge to public health. In this study, we investigated the development of resistance in Pseudomonas aeruginosa [...] Read more.
Bacterial persisters are dormant cells that survive antibiotic treatment, serving as a reservoir for the emergence of resistant mutations. The evolution of antibiotic resistance poses a significant challenge to public health. In this study, we investigated the development of resistance in Pseudomonas aeruginosa persister cells by exposing the reference strain PA14 to meropenem and tracked the emergence of resistance mutations over serial passages. Whole-genome sequencing of the populations or individual resistant strains revealed evolutionary trajectories. In the initial passages, low-level meropenem-resistant mutants harbored various mutations, accompanied by increasing population survival. Then, mutations in the oprD gene appeared, followed by mutation in the mexR gene in most of the cells, leading to high-level meropenem resistance and collateral resistance to ciprofloxacin. Our study provides insights into the evolutionary pathways of P. aeruginosa under lethal antibiotic pressure, highlighting the dynamic interplay between persister cells and the emergence of resistance mutations. Full article
(This article belongs to the Special Issue Bacterial Pathogenesis and Host Immune Responses)
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