Bioengineering Strategies for Developing Vaccines

A special issue of Vaccines (ISSN 2076-393X).

Deadline for manuscript submissions: 30 June 2026 | Viewed by 5891

Special Issue Editors


E-Mail Website
Guest Editor
Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China
Interests: vaccine immunology; viral immunology; B cells; host–virus interactions

E-Mail Website
Guest Editor
Center of Biotherapy, Zhongshan Hospital, Fudan University, Shanghai 200032, China
Interests: vaccine immunology; viral immunology; mRNA; T cells; immunodominance; antigen design

Special Issue Information

Dear Colleagues,

In the past few years, we have witnessed a remarkable stride in vaccine development, which was driven by a rapid response to the SARS-CoV-2 pandemic. Along with this evolution, bioengineering has seen more integration into vaccine development than ever before. Genetic engineering enables the creation of antigens that are tailored to elicit stronger desired immune responses. Gene editing has become a common tool in exploring vaccine platforms, while biomaterials and nanotechnology continue to add new ingredients to improve antigen stability, as well as vaccine release and delivery. Additionally, microfluidic devices have emerged as an effective way to enhance immune response in a targeted, controlled manner.

For this Special Issue, we are calling for research papers and reviews related to the bioengineering of vaccines, including, but not limited to, antigen engineering, vaccine platform development, vaccine delivery systems, and the application of artificial intelligence to vaccine design. We hope this Special Issue provides an opportunity to learn about updated bioengineering advances in vaccine development, both innovative methods and real-world applications, for advancing vaccine efficacy and safety.

Prof. Dr. Chen Zhao
Dr. Jiayi Shu
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Vaccines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • vaccine platform
  • antigen design
  • targeted delivery
  • mRNA
  • nanoparticle
  • artificial intelligence

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • Reprint: MDPI Books provides the opportunity to republish successful Special Issues in book format, both online and in print.

Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

Jump to: Review

21 pages, 4313 KB  
Article
Chimeric Virus-like Particles Formed by the Coat Proteins of Single-Stranded RNA Phages Beihai32 and PQ465, Simultaneously Displaying the M2e Peptide and the Stalk HA Peptide from Influenza a Virus, Elicit Humoral and T-Cell Immune Responses in Mice
by Egor A. Vasyagin, Anna A. Zykova, Elena A. Blokhina, Olga O. Ozhereleva, Liudmila A. Stepanova, Marina A. Shuklina, Sergey A. Klotchenko, Eugenia S. Mardanova and Nikolai V. Ravin
Vaccines 2025, 13(11), 1117; https://doi.org/10.3390/vaccines13111117 - 30 Oct 2025
Viewed by 401
Abstract
Background: The extracellular domain of the M2 protein (M2e) and the conserved region of the second subunit of the hemagglutinin (HA2, 76–130 а.а.) of the influenza A virus, could be used to develop broad-spectrum influenza vaccines. However, these antigens have low immunogenicity and [...] Read more.
Background: The extracellular domain of the M2 protein (M2e) and the conserved region of the second subunit of the hemagglutinin (HA2, 76–130 а.а.) of the influenza A virus, could be used to develop broad-spectrum influenza vaccines. However, these antigens have low immunogenicity and require the use of special carriers to enhance it. Virus-like particles (VLPs) formed from viral capsid proteins are among the most effective carriers. Methods: In this work, we obtained and characterized VLPs based on capsid proteins (CPs) of single-stranded RNA bacteriophages Beihai32 and PQ465, simultaneously displaying M2e and HA2 peptides. Results: Fusion proteins expressed in Escherichia coli formed spherical VLPs of about 30 nm in size. Subcutaneous immunization of mice with chimeric VLPs elicited a robust humoral immune response against M2e and the whole influenza A virus, and promoted the formation of cytokine-secreting antigen-specific CD4+ and CD8+ effector memory T cells. Conclusions: VLPs based on CPs of phages Beihai32 and PQ465 carrying conserved peptides M2e and HA2 of the influenza A virus can be used for the development of universal influenza vaccines. Full article
(This article belongs to the Special Issue Bioengineering Strategies for Developing Vaccines)
Show Figures

Figure 1

Review

Jump to: Research

67 pages, 4242 KB  
Review
Bioengineering Outer-Membrane Vesicles for Vaccine Development: Strategies, Advances, and Perspectives
by Ayesha Zahid, Hazrat Ismail, Jennifer C. Wilson and I. Darren Grice
Vaccines 2025, 13(7), 767; https://doi.org/10.3390/vaccines13070767 - 20 Jul 2025
Cited by 2 | Viewed by 4771
Abstract
Outer-membrane vesicles (OMVs), naturally secreted by Gram-negative bacteria, have gained recognition as a versatile platform for the development of next-generation vaccines. OMVs are essential contributors to bacterial pathogenesis, horizontal gene transfer, cellular communication, the maintenance of bacterial fitness, and quorum sensing. Their intrinsic [...] Read more.
Outer-membrane vesicles (OMVs), naturally secreted by Gram-negative bacteria, have gained recognition as a versatile platform for the development of next-generation vaccines. OMVs are essential contributors to bacterial pathogenesis, horizontal gene transfer, cellular communication, the maintenance of bacterial fitness, and quorum sensing. Their intrinsic immunogenicity, adjuvant properties, and scalability establish OMVs as potent tools for combating infectious diseases and cancer. Recent advancements in genetic engineering and biotechnology have further expanded the utility of OMVs, enabling the incorporation of multiple epitopes and antigens from diverse pathogens. These developments address critical challenges such as antigenic variability and co-infections, offering broader immune coverage and cost-effective solutions. This review explores the unique structural and immunological properties of OMVs, emphasizing their capacity to elicit robust immune responses. It critically examines established and emerging engineering strategies, including the genetic engineering of surface-displayed antigens, surface conjugation, glycoengineering, nanoparticle-based OMV engineering, hybrid OMVs, and in situ OMV production, among others. Furthermore, recent advancements in preclinical research on OMV-based vaccines, including synthetic OMVs, OMV-based nanorobots, and nanodiscs, as well as emerging isolation and purification methods, are discussed. Lastly, future directions are proposed, highlighting the potential integration of synthetic biology techniques to accelerate research on OMV engineering. Full article
(This article belongs to the Special Issue Bioengineering Strategies for Developing Vaccines)
Show Figures

Graphical abstract

Back to TopTop