Electrospinning Technologies for Biomedical and Biotechnological Applications—Volume II

A special issue of Technologies (ISSN 2227-7080). This special issue belongs to the section "Innovations in Materials Processing".

Deadline for manuscript submissions: closed (31 January 2024) | Viewed by 14924

Special Issue Editor


E-Mail Website1 Website2
Guest Editor
1. Head of the Laboratory of Natural Polymers, Institute of Macromolecular Compounds of the Russian Academy of Sciences, St. Petersburg, Russia
2. Head of the Analytical Chemistry Department, Almazov National Medical Research Centre, St. Petersburg, Russia
Interests: polysaccharides; biomaterials; tissue engineering; drug delivery; gene delivery; nanomedicine; nanocomposites; electrospinning
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Continuing the Special Issue “Electrospinning Technologies for Biomedical and Biotechnological Applications”, we are pleased to open a new Special Issue, “Electrospinning Technologies for Biomedical and Biotechnological Applications—Volume II”. This new Special Issue is motivated by the observed growing interest in the design, fabrication, and application of electrospun nanofiber materials in the fields of biomedicine and biotechnology. It aims to provide broad coverage of the research progress, as well as up-to-date reviews addressing the various fundamental and applied problems of using electrospinning techniques for multiple bio-applications.

In this Special Issue, we seek contributions from active experts discussing the improvements in electrospinning technologies, and innovations in electrospun biomaterials, including applications in tissue engineering, regenerative medicine, wound healing, drug and gene delivery, filtration, and enzyme supports, among others. We intend for this Special Issue to provide a unique platform for the diffusion of new concepts and bio-applications of electrospun nonwoven materials, so as to continue the motivation and inspiration for further research in this newly invigorated field.

Dr. Yury A. Skorik
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Technologies is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • electrospinning
  • non-wovens
  • nanomaterials
  • tissue engineering
  • regenerative medicine
  • drug delivery
  • gene delivery
  • wound healing
  • filtration
  • enzyme immobilization

Related Special Issue

Published Papers (8 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

Jump to: Review

25 pages, 2932 KiB  
Article
Pitch/Metal Oxide Composite Fibers via Electrospinning for Environmental Applications
by Bayan Kaidar, Gaukhar Smagulova, Aigerim Imash, Aruzhan Keneshbekova, Akram Ilyanov and Zulkhair Mansurov
Technologies 2023, 11(6), 156; https://doi.org/10.3390/technologies11060156 - 07 Nov 2023
Cited by 1 | Viewed by 1657
Abstract
This study investigates the synthesis and application of composite electrospun fibers incorporating coal tar pitch (CTP) and various nanomaterial additives, with a specific focus on their potential for eco-bio-applications. The research underscores the environmentally viable aspects of CTP following a thermal treatment process [...] Read more.
This study investigates the synthesis and application of composite electrospun fibers incorporating coal tar pitch (CTP) and various nanomaterial additives, with a specific focus on their potential for eco-bio-applications. The research underscores the environmentally viable aspects of CTP following a thermal treatment process that eliminates volatile components and sulfur, rendering it amenable for fiber electrospinning and subsequent carbonization. Composite fibers were fabricated by integrating CTP with nanomaterials, including nickel oxide (NiO), titanium dioxide (TiO2), activated carbon (AC), and magnetite (Fe3O4). The C/NiO composite fibers exhibit notable acetone sensing capabilities, specifically displaying a rapid response time of 40.6 s to 100 ppm acetone at 220 °C. The C/TiO2 composite fibers exhibit a distinct “beads-on-a-string” structure and demonstrate a high efficiency of 96.13% in methylene blue decomposition, highlighting their potential for environmental remediation applications. Additionally, the C/AC composite fibers demonstrate effective adsorption properties, efficiently removing manganese (II) ions from aqueous solutions with an 88.62% efficiency, thereby suggesting their utility in water purification applications. This research employs an interdisciplinary approach by combining diverse methods, approaches, and materials, including the utilization of agricultural waste materials such as rice husks, to create composite materials with multifaceted applications. Beyond the immediate utility of the composite fibers, this study emphasizes the significance of deploying environmentally responsible materials and technologies to address pressing eco-bio-challenges. Full article
Show Figures

Figure 1

15 pages, 6974 KiB  
Article
Approaches to Obtaining Water-Insoluble Fibrous Matrices from Regenerated Fibroin
by Nataliya Kildeeva, Nikita Sazhnev, Maria Drozdova, Vasilina Zakharova, Evgeniya Svidchenko, Nikolay Surin and Elena Markvicheva
Technologies 2023, 11(5), 146; https://doi.org/10.3390/technologies11050146 - 19 Oct 2023
Viewed by 1551
Abstract
Silk fibroin (SF) holds promise for the preparation of matrices for tissue engineering and regenerative medicine or for the development of drug delivery systems. Regenerated fibroin from Bombyx mori cocoons is water-soluble and can be processed into scaffolds of various forms, such as [...] Read more.
Silk fibroin (SF) holds promise for the preparation of matrices for tissue engineering and regenerative medicine or for the development of drug delivery systems. Regenerated fibroin from Bombyx mori cocoons is water-soluble and can be processed into scaffolds of various forms, such as fibrous matrices, using the electrospinning method. In the current study, we studied the correlation between concentrations of fibroin aqueous solutions and their properties, in order to obtain electrospun mats for tissue engineering. Two methods were used to prevent solubility in fibroin-based matrices: The conversion of fibroin to the β-conformation via treatment with an ethanol solution and chemical cross-linking with genipin (Gp). The interaction of Gp with SF led to the appearance of a characteristic blue color but did not lead to the gelation of solutions. To speed up the cross-linking reaction with Gp, we propose using chitosan-containing systems and modifying fibrous materials via treatment with a solution of Gp in 80% ethanol. It was shown that the composition of fibroin with chitosan contributes to an improved water resistance, reduces defective material, and leads to a decrease in the diameter of the fibers. The electrospun fiber matrices based on regenerated fibroin modified by cross-linking with genipin in water–alcohol solutions were shown to promote cell adhesion, spreading, and growth and, therefore, could hold promise for tissue engineering. Full article
Show Figures

Figure 1

18 pages, 6018 KiB  
Article
Preparation and Characterization of Thermoresponsive Polymer Scaffolds Based on Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) for Cell Culture
by Gilyana K. Kazakova, Victoria S. Presniakova, Yuri M. Efremov, Svetlana L. Kotova, Anastasia A. Frolova, Sergei V. Kostjuk, Yury A. Rochev and Peter S. Timashev
Technologies 2023, 11(5), 145; https://doi.org/10.3390/technologies11050145 - 18 Oct 2023
Cited by 1 | Viewed by 1636
Abstract
In the realm of scaffold-free cell therapies, there is a questto develop organotypic three-dimensional (3D) tissue surrogates in vitro, capitalizing on the inherent ability of cells to create tissues with an efficiency and sophistication that still remains unmatched by human-made devices. In this [...] Read more.
In the realm of scaffold-free cell therapies, there is a questto develop organotypic three-dimensional (3D) tissue surrogates in vitro, capitalizing on the inherent ability of cells to create tissues with an efficiency and sophistication that still remains unmatched by human-made devices. In this study, we explored the properties of scaffolds obtained by the electrospinning of a thermosensitive copolymer, poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) (P(NIPAM-co-NtBA)), intended for use in such therapies. Two copolymers with molecular weights of 123 and 137 kDa and a content of N-tert-butylacrylamide of ca. 15 mol% were utilized to generate 3D scaffolds via electrospinning. We examined the morphology, solution viscosity, porosity, and thickness of the spun matrices as well as the mechanical properties and hydrophobic–hydrophilic characteristics of the scaffolds. Particular attention was paid to studying the influence of the thermosensitive polymer’s molecular weight and dispersity on the resultant scaffolds’ properties and the role of electroforming parameters on the morphology and mechanical characteristics of the scaffolds. The cytotoxicity of the copolymers and interaction of cells with the scaffolds were also studied. Our findings provide significant insight into approaches to optimizing scaffolds for specific cell cultures, thereby offering new opportunities for scaffold-free cell therapies. Full article
Show Figures

Figure 1

22 pages, 8407 KiB  
Article
Effect of PLGA Concentration in Electrospinning Solution on Biocompatibility, Morphology and Mechanical Properties of Nonwoven Scaffolds
by Arsalan D. Badaraev, Tuan-Hoang Tran, Anastasia G. Drozd, Evgenii V. Plotnikov, Gleb E. Dubinenko, Anna I. Kozelskaya, Sven Rutkowski and Sergei I. Tverdokhlebov
Technologies 2023, 11(5), 137; https://doi.org/10.3390/technologies11050137 - 05 Oct 2023
Viewed by 1745
Abstract
In this work, the effects of weight concentration on the properties of poly(lactide-co-glycolide) polymeric scaffolds prepared by electrospinning are investigated, using four different weight concentrations of poly(lactide-co-glycolide) for the electrospinning solutions (2, 3, 4, 5 wt.%). With increasing concentration of poly(lactide-co-glycolide) in the [...] Read more.
In this work, the effects of weight concentration on the properties of poly(lactide-co-glycolide) polymeric scaffolds prepared by electrospinning are investigated, using four different weight concentrations of poly(lactide-co-glycolide) for the electrospinning solutions (2, 3, 4, 5 wt.%). With increasing concentration of poly(lactide-co-glycolide) in the electrospinning solutions, their viscosity increases significantly. The average fiber diameter of the scaffolds also increases with increasing concentration. Moreover, the tensile strength and maximum elongation at break of the scaffold increase with increasing electrospinning concentration. The prepared scaffolds have hydrophobic properties and their wetting angle does not change with the concentration of the electrospinning solution. All poly(lactide-co-glycolide) scaffolds are non-toxic toward fibroblasts of the cell line 3T3-L1, with the highest numbers of cells observed on the surface of scaffolds prepared from the 2-, 3- and 4-wt.% electrospinning solutions. The results of the analysis of mechanical and biological properties indicate that the poly(lactide-co-glycolide) scaffolds prepared from the 4 wt.% electrospinning solution have optimal properties for future applications in skin tissue engineering. This is due to the fact that the poly(lactide-co-glycolide) scaffolds prepared from the 2 wt.% and 3 wt.% electrospinning solution exhibit low mechanical properties, and 5 wt.% have the lowest porosity values, which might be the cause of their lowest biological properties. Full article
Show Figures

Figure 1

16 pages, 3430 KiB  
Article
The Influence of Technological Factors and Polar Molecules on the Structure of Fibrillar Matrices Based on Ultrafine Poly-3-hydroxybutyrate Fibers Obtained via Electrospinning
by Anatoly A. Olkhov, Polina M. Tyubaeva, Yulia N. Zernova, Valery S. Markin, Regina Kosenko, Anna G. Filatova, Kristina G. Gasparyan and Alexey L. Iordanskii
Technologies 2023, 11(5), 118; https://doi.org/10.3390/technologies11050118 - 06 Sep 2023
Cited by 2 | Viewed by 1213
Abstract
The article examines the regularities of structure formation of ultrafine fibers based on poly-3-hydroxybutyrat under the influence of technological (electrical conductivity, viscosity), molecular (molecular weight), and external factors (low-molecular and nanodispersed substances of different chemical natures). Systems with polar substances are characterized by [...] Read more.
The article examines the regularities of structure formation of ultrafine fibers based on poly-3-hydroxybutyrat under the influence of technological (electrical conductivity, viscosity), molecular (molecular weight), and external factors (low-molecular and nanodispersed substances of different chemical natures). Systems with polar substances are characterized by the presence of intermolecular interactions and the formation of a more perfect crystalline fiber structure. Changes in technological and molecular characteristics affect the fiber formation process, resulting in alterations in the morphology of the nonwoven fabric, fiber geometry, and supramolecular fiber structure. Polymer molecular weight, electrical conductivity, and solution viscosity influence fiber formation and fiber diameter. The fiber structure is heterogeneous, consisting of both crystalline and non-equilibrium amorphous phases. This article shows that with an increase in the molecular weight and concentration of the polymer, the diameter of the fiber increases. At the same time, the increase in the productivity of the electrospinning process does not affect the fiber geometry. The chemical structure of the solvent and the concentration of polar substances play a decisive role in the formation of fibers of even geometry. As the polarity of the solvent increases, the intermolecular interaction with the polar groups of poly-3-hydroxybutyrate increases. As a result of this interaction, the crystallites are improved, and the amorphous phase of the polymer is compacted. The action of polar molecules on the polymer is similar to the action of polar nanoparticles. They increase crystallinity via a nucleation mechanism. This is significant in the development of matrix-fibrillar systems for drug delivery, bioactive substances, antiseptics, tissue engineering constructs, tissue engineering scaffolds, artificial biodegradable implants, sorbents, and other applications. Full article
Show Figures

Graphical abstract

23 pages, 10373 KiB  
Article
Biodegradable Polyhydroxyalkanoates Formed by 3- and 4-Hydroxybutyrate Monomers to Produce Nanomembranes Suitable for Drug Delivery and Cell Culture
by Tatiana G. Volova, Aleksey V. Demidenko, Anastasiya V. Murueva, Alexey E. Dudaev, Ivan Nemtsev and Ekaterina I. Shishatskaya
Technologies 2023, 11(4), 106; https://doi.org/10.3390/technologies11040106 - 07 Aug 2023
Viewed by 1678
Abstract
Biodegradable polyhydroxyalkanoates, biopolymers of microbiological origin, formed by 3- and 4-hydroxybutyrate monomers P(3HB-co-4HB), were used to obtain nanomembranes loaded with drugs as cell carriers by electrospinning. Resorbable non-woven membranes from P(3HB-co-4HB) loaded with ceftazidime, doripinem, and actovegin have been obtained. The loading of [...] Read more.
Biodegradable polyhydroxyalkanoates, biopolymers of microbiological origin, formed by 3- and 4-hydroxybutyrate monomers P(3HB-co-4HB), were used to obtain nanomembranes loaded with drugs as cell carriers by electrospinning. Resorbable non-woven membranes from P(3HB-co-4HB) loaded with ceftazidime, doripinem, and actovegin have been obtained. The loading of membranes with drugs differently affected the size of fibers and the structure of membranes, and in all cases increased the hydrophilicity of the surface. The release of drugs in vitro was gradual, which corresponded to the Higuchi and Korsmeyer-Peppas models. Antibiotic-loaded membranes showed antibacterial activity against S. aureus and E. coli, in which growth inhibition zones were 41.7 ± 1.1 and 38.6 ± 1.7 mm for ceftazidime and doripinem, respectively. The study of the biological activity of membranes in the NIH 3T3 mouse fibroblast culture based on the results of DAPI and FITC staining of cells, as well as the MTT test, did not reveal a negative effect despite the presence of antibiotics in them. Samples containing actovegin exhibit a stimulating effect on fibroblasts. Biodegradable polyhydroxyalkanoates formed by 3-hydroxybutyrate and 4-hydroxybutyrate monomers provide electrospinning non-woven membranes suitable for long-term delivery of drugs and cultivation of eukaryotic cells, and are promising for the treatment of wound defects complicated by infection. Full article
Show Figures

Graphical abstract

18 pages, 7297 KiB  
Article
PHB/PEG Nanofiber Mat Obtained by Electrospinning and Their Performances
by Nguyen Hong Thanh, Roman Olekhnovich, Vera Sitnikova, Arina Kremleva, Petr Snetkov and Mayya Uspenskaya
Technologies 2023, 11(2), 48; https://doi.org/10.3390/technologies11020048 - 24 Mar 2023
Cited by 8 | Viewed by 2335
Abstract
In this work, a nanofiber mat based on PHB/PEG with various PEG contents was obtained by electrospinning process. The thermal and mechanical properties of the PHB/PEG nanofiber mat were investigated. In addition, PHB/PEG nanofiber mats were characterized by Fourier transforms infrared spectroscopy (FTIR), [...] Read more.
In this work, a nanofiber mat based on PHB/PEG with various PEG contents was obtained by electrospinning process. The thermal and mechanical properties of the PHB/PEG nanofiber mat were investigated. In addition, PHB/PEG nanofiber mats were characterized by Fourier transforms infrared spectroscopy (FTIR), differential scanning calorimetry, thermogravimetric analysis, X-ray diffraction, and water contact angle measurement. It was shown that, by increasing the PEG contents from 1 to 4%, the average diameter of PHB nanofibers decreased from 1177 nm to 1101 nm, corresponding to 2% PEG, then the diameter of the fiber increased again from 1101 nm to 1136 nm, corresponding to 4% PEG. Tensile strength increased from 3.6 MPa to 4.4 MPa, then decreased from 4.4 MPa to 2.9 MPa. Thermogravimetric analysis showed a difference in the process of thermal degradation of nanofiber mats. The degree of crystallinity measured by XRD and DSC methods gives different values at some points. The results demonstrated that adding PEG improved the mechanical properties, hydrophobicity, porosity, and thermal stability of the PHB fiber mat, which showed that the PHB/PEG nanofiber mat has great potential for air filtration or water filtration. Full article
Show Figures

Figure 1

Review

Jump to: Research

28 pages, 2632 KiB  
Review
Green Electrospun Nanofibers for Biomedicine and Biotechnology
by Elyor Berdimurodov, Omar Dagdag, Khasan Berdimuradov, Wan Mohd Norsani Wan Nik, Ilyos Eliboev, Mansur Ashirov, Sherzod Niyozkulov, Muslum Demir, Chinmurot Yodgorov and Nizomiddin Aliev
Technologies 2023, 11(5), 150; https://doi.org/10.3390/technologies11050150 - 23 Oct 2023
Cited by 1 | Viewed by 2204
Abstract
Green electrospinning harnesses the potential of renewable biomaterials to craft biodegradable nanofiber structures, expanding their utility across a spectrum of applications. In this comprehensive review, we summarize the production, characterization and application of electrospun cellulose, collagen, gelatin and other biopolymer nanofibers in tissue [...] Read more.
Green electrospinning harnesses the potential of renewable biomaterials to craft biodegradable nanofiber structures, expanding their utility across a spectrum of applications. In this comprehensive review, we summarize the production, characterization and application of electrospun cellulose, collagen, gelatin and other biopolymer nanofibers in tissue engineering, drug delivery, biosensing, environmental remediation, agriculture and synthetic biology. These applications span diverse fields, including tissue engineering, drug delivery, biosensing, environmental remediation, agriculture, and synthetic biology. In the realm of tissue engineering, nanofibers emerge as key players, adept at mimicking the intricacies of the extracellular matrix. These fibers serve as scaffolds and vascular grafts, showcasing their potential to regenerate and repair tissues. Moreover, they facilitate controlled drug and gene delivery, ensuring sustained therapeutic levels essential for optimized wound healing and cancer treatment. Biosensing platforms, another prominent arena, leverage nanofibers by immobilizing enzymes and antibodies onto their surfaces. This enables precise glucose monitoring, pathogen detection, and immunodiagnostics. In the environmental sector, these fibers prove invaluable, purifying water through efficient adsorption and filtration, while also serving as potent air filtration agents against pollutants and pathogens. Agricultural applications see the deployment of nanofibers in controlled release fertilizers and pesticides, enhancing crop management, and extending antimicrobial food packaging coatings to prolong shelf life. In the realm of synthetic biology, these fibers play a pivotal role by encapsulating cells and facilitating bacteria-mediated prodrug activation strategies. Across this multifaceted landscape, nanofibers offer tunable topographies and surface functionalities that tightly regulate cellular behavior and molecular interactions. Importantly, their biodegradable nature aligns with sustainability goals, positioning them as promising alternatives to synthetic polymer-based technologies. As research and development continue to refine and expand the capabilities of green electrospun nanofibers, their versatility promises to advance numerous applications in the realms of biomedicine and biotechnology, contributing to a more sustainable and environmentally conscious future. Full article
Show Figures

Figure 1

Back to TopTop