Approaches to Obtaining Water-Insoluble Fibrous Matrices from Regenerated Fibroin
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Preparation of Regenerated Fibroin
2.3. Preparation of Fibroin Solutions and Mixed Solutions of Fibroin and Chitosan
2.4. Kinetics of Change in Viscosity of SF Solutions
2.5. Measurement of Turbidity
2.6. Electrical Conductivity of Fibroin Solutions
2.7. Thermogravimetric Analysis
2.8. Solubility of SF Fibrous Matrices
2.9. Study of Gel Formation in Fibroin Solutions When Cross-Linked with Genipin
2.10. Preparation of Fibrous Matrices by Electrospinning
2.11. Modification of Fiber Matrices
2.12. Study of Fibroin Matrix Morphology Using Confocal Laser Scanning Microscopy
2.13. Study of the Structures of Fibrous Matrices by Confocal Microscopy
2.14. Cell Culture
2.15. Cell Cultivation in Fibrous Matrices
2.16. Study of Cell Proliferation in Fibrous Matrices
2.17. Morphology of Cells after Three Days of Cultivation in Fibrous Matrices
3. Results
3.1. Effect of Silk Fibroin Solution Concentration on Fibrous Matrices from Regenerated Fibroin
3.2. Hydrophobization of Electrospun SF Fibers with An Ethanol Solution
3.3. Use of a Genipin Cross-Linking Reagent for Preparation of Water-Insoluble Fibrous Matrices from Regenerated Fibroin
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fibroin Solution Concentration, % | pH | Conductivity ϰ, mS/cm | Dynamic Viscosity η, mPa⋅s |
---|---|---|---|
10 | 6.80 | 3.6 | 20.4 |
20 | 6.90 | 6.4 | 44.2 |
30 | 6.93 | 9.8 | 67.2 |
Fibroin Solution Concentration, % w/w | Electrospinning Voltage E, kV | Characteristics of the Electrospinning Process | AFM Image | Fiber Diameter, μm |
---|---|---|---|---|
10 | 22.2–25.8 | Stable | 0.61 ± 0.22 | |
20 | 23.0–26.4 | Stable | 2.80 ± 0.20 | |
30 | 24.1–28.0 | Unstable | 2.20 ± 0.80 |
Concentration,% | Fibroin/Chitosan Ratio, g/g | pH | Conductivity ϰ, mS/cm | Dynamic Viscosity η, mPa⋅s | |
---|---|---|---|---|---|
Fibroin | Chitosan | ||||
20 | - | 1:0 | 6.90 | 6.4 | 44.13 |
10 | 2 | 5:1 | 4.84 | 27.3 | 62.21 |
Fibrous Matrix Composition | Morphology of the Source Electrospun Fibrous Matrices | Morphology of the Fibrous Matrices Washed with PBS (pH 7.4) | Morphology of the Fibrous Matrices after 3 Days of Cultivation of hTERT-MSCs |
---|---|---|---|
100% fibroin | |||
Fibroin/Chitosan, 5:1 |
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Kildeeva, N.; Sazhnev, N.; Drozdova, M.; Zakharova, V.; Svidchenko, E.; Surin, N.; Markvicheva, E. Approaches to Obtaining Water-Insoluble Fibrous Matrices from Regenerated Fibroin. Technologies 2023, 11, 146. https://doi.org/10.3390/technologies11050146
Kildeeva N, Sazhnev N, Drozdova M, Zakharova V, Svidchenko E, Surin N, Markvicheva E. Approaches to Obtaining Water-Insoluble Fibrous Matrices from Regenerated Fibroin. Technologies. 2023; 11(5):146. https://doi.org/10.3390/technologies11050146
Chicago/Turabian StyleKildeeva, Nataliya, Nikita Sazhnev, Maria Drozdova, Vasilina Zakharova, Evgeniya Svidchenko, Nikolay Surin, and Elena Markvicheva. 2023. "Approaches to Obtaining Water-Insoluble Fibrous Matrices from Regenerated Fibroin" Technologies 11, no. 5: 146. https://doi.org/10.3390/technologies11050146