- Article
Bioavailable Forms of Heavy Metals and Se in Soil in the Vicinity of the Pechenganikel Smelting Plant and the Relationship with Mineral Composition and Antioxidant Status of Biocrusts
- Nadezhda Golubkina,
- Sergey Sheshnitsan and
- Andrew Koshevarov
- + 8 authors
The evaluation of bioavailable forms of heavy metals in zones of anthropogenic pollution is the basis of ecological risk assessment. The characterization of the consequences of the operation of the Pechenganikel smelting plant was carried out using AAS and two methods of soil bioavailable forms of heavy metal extraction (3% nitric acid and ammonium acetate buffer with pH 4.8) along three directions from the plant, corresponding to the wind prevalence. Buffer extraction provided more significant correlations between Ni, Co, Cu, Pb, and Zn, compared to nitric acid application, indicating a negative correlation between soil Cu, Co, and the distance from the plant, while no significant correlations were recorded for nitric acid extracts. A higher significant correlation number arose between soil elements in buffer extracts along the N-E direction than the S-W one. In the former direction, the number of the mentioned correlations decreased according to the following sequence: Zn (6) > Cu (5) > Se and Co (4) > Ni and Fe (3); in nitric acid extract, only significant correlations of Cu, Zn, and Se with Co and Ni were recorded. Biocrust formation was revealed only along the N-E direction, characterized by unexpected high Se concentrations and intensive correlation between Zn and all the elements extracted by the buffer. Biocrust accumulated high levels of all the elements tested and showed antioxidant activity and polyphenol content significantly correlated with soil organic matter. The biocrust mineral content demonstrated a complex relationship with soil Fe, Cu (buffer extract), and Se, as well as Co and Zn (nitric acid extract). Application of linear mixed-effects modelling and transfer factor analysis indicate that biocrusts may serve as effective bioindicators of both absolute metal contamination and its bioavailable fractions. The results indicate the expediency of using both methods of soil extraction for assessing the ecological risk and soil–biocrust relationships.
14 October 2025



