Special Issue "Advances in Optical, Fluorescent and Luminescent Biosensors"
Deadline for manuscript submissions: 30 September 2021.
Interests: protein structure/function; fluorescence sensors; enzyme immobilization; bioreactors; biosensors; pesticide sensing
Special Issues and Collections in MDPI journals
Interests: protein-protein interactions; colorimetric immunosensors; bioinorganic oxidations; protein design; protein chromatography; mass spectrometry
Special Issues and Collections in MDPI journals
Nature masters the art of sensing by the combination of different optical, thermal, chemical, and mechanical external stimuli. In general, each one of them is recognized by specific receptor and transducer molecules that act as antennas in delineating and communicating with the environment. The development of bio-inspired sensors (chemical and biochemical) give us the opportunity to learn this lesson, using such molecular tools (e.g., reporter enzymes, fluorescent and chemiluminescent proteins, antibodies, DNA-binding proteins, aptamers) and viable cells and microorganisms for specific tasks.
Recently, a wide range of applications have been recognized in which biosensing plays a key role, as it enables fast, reliable, and continuous detection in point-of-care diagnostics, persistent organic pollutant monitoring, intracellular sensing, and futuristic fitness monitoring and wearable chemical sensors. Chemical and biological markers may be found in different matrices, such as tears, saliva, sweat, ISF, blood, and exhaled breath, as well as sea and tap water, soil, and atmosphere.
In this Special Issue, we focus our attention on recent advances in optical, fluorescent, and luminescent biosensors. This class of devices may be either coupled to electrochemistry or material science in order to improve their performance, leading to electrochemiluminescence sensors, active/smart surfaces, and nano-sized stand-alone sensors. Original manuscripts as well as reviews of the current state of the art are welcome for submission.
Dr. Ferdinando Febbraio
Dr. Marco Chino
Prof. Rabeay Hassan
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- fluorescent sensors
- colorimetric sensors
- luminescent sensors
- microbial sensors
- protein engineering
- synthetic biology
- complex matrix
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Calibration of Plate Reader Fluorescent Measurements
Authors: Jesús Andrés Picó Marco
Affiliation: Professor of Automatic Control, Universitat Politècnica de València
Abstract: Fluorescent proteins are widely used as activity reporters of target proteins expressed by engineered genetic circuits in cells. A plate reader is the instrument that provides bulk fluorescence measurements of cell cultures over a period of time. However, these measurements are expressed in arbitrary units, which hampers the comparison of results even if they are obtained in the same lab. One of the key steps towards findable, accessible, interoperable, and reusable (FAIR) data and the standardization in Synthetic Biology is to make data easily usable and comparable. In this work, we propose a two-step protocol. The goal of the first step is to fit the unit conversion model, which transforms fluorescence measurements, provided in arbitrary units, to concentration units. The model calibration also accounts for the effect of the Gain parameter on the recorded data. The goal of the second step is to evaluate the quality of the measurement system (in terms of bias, linearity, repeatability and reproducibility) after the correction proposed in the first step. Thus, only if the measurement system is statistically reliable, our conversion model will be totally validated. Moreover, the measurement system analysis is used to estimate the uncertainty for new measures, establishing confidence intervals for the prediction of new data. This becomes crucial to evaluate the statistical feasibility and relevance of any procedure involving the acquisition of data with an inherent variability. All the functions used to build the model, to exploit it with new data and to perform the measurement system analysis, have been implemented in Matlab and are available in GitHub repository.