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Open AccessArticle

Gaussia Luciferase as a Reporter for Quorum Sensing in Staphylococcus aureus

1
School of Life Sciences, Biodiscovery Institute, University of Nottingham, University Park, Nottingham NG7 2RD, UK
2
School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough LE12 5RD, UK
3
College of Chemical Engineering, Huaqiao University, 668 Jimei Blvd, Xiamen 361021, China
4
School of Pharmacy, Biodiscovery Institute, University of Nottingham, University Park, Nottingham NG7 2RD, UK
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Present address: Sygnature Discovery Limited, BioCity, Pennyfoot Street, Nottingham NG1 1GR, UK.
Sensors 2020, 20(15), 4305; https://doi.org/10.3390/s20154305
Received: 30 June 2020 / Revised: 23 July 2020 / Accepted: 30 July 2020 / Published: 1 August 2020
(This article belongs to the Special Issue Advances in Optical, Fluorescent and Luminescent Biosensors)
Gaussia luciferase (GLuc) is a secreted protein with significant potential for use as a reporter of gene expression in bacterial pathogenicity studies. To date there are relatively few examples of its use in bacteriology. In this study we show that GLuc can be functionally expressed in the human pathogen Staphylococcus aureus and furthermore show that it can be used as a biosensor for the agr quorum sensing (QS) system which employs autoinducing peptides to control virulence. GLuc was linked to the P3 promoter of the S. aureusagr operon. Biosensor strains were validated by evaluation of chemical agent-mediated activation and inhibition of agr. Use of GLuc enabled quantitative assessment of agr activity. This demonstrates the utility of Gaussia luciferase for in vitro monitoring of agr activation and inhibition. View Full-Text
Keywords: Gaussia luciferase; quorum sensing; Staphylococcus aureus; agr Gaussia luciferase; quorum sensing; Staphylococcus aureus; agr
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MDPI and ACS Style

Blower, I.; Tong, C.; Sun, X.; Murray, E.; Luckett, J.; Chan, W.; Williams, P.; Hill, P. Gaussia Luciferase as a Reporter for Quorum Sensing in Staphylococcus aureus. Sensors 2020, 20, 4305.

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