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Open AccessTechnical Note

Development and Evaluation of a Quantitative Fluorescent Lateral Flow Immunoassay for Cystatin-C, a Renal Dysfunction Biomarker

1
Healthcare Technology Innovation Centre, Indian Institute of Technology Madras, Chennai 600113, India
2
Department of Chemistry, Carleton University, Ottawa, ON K1S 5B6, Canada
3
Department of Electrical Engineering, Indian Institute of Technology, Madras, Chennai 600113, India
*
Authors to whom correspondence should be addressed.
Academic Editor: Ferdinando Febbraio
Sensors 2021, 21(9), 3178; https://doi.org/10.3390/s21093178
Received: 16 March 2021 / Revised: 18 April 2021 / Accepted: 28 April 2021 / Published: 3 May 2021
(This article belongs to the Special Issue Advances in Optical, Fluorescent and Luminescent Biosensors)
The diagnosis, prognosis, and control of chronic kidney disease rely on an understanding of the glomerular filtration rate (GFR). The renal clearance of the cystatin-C is closely associated with the GFR. Cystatin-C is a more suitable GFR marker than the commonly used creatinine. General techniques for cystatin-C calculation, such as particle-enhanced turbidimetric and nephelometric assay, are time-consuming and tedious. Here, we propose a rapid, quantitative immunoassay for the detection of cystatin-C. A fluorescence-based lateral-flow kit was developed in a sandwich format by using a monoclonal antibody. A Linear calibration was obtained over the clinical diagnostic range of 0.023–32 µg/mL and the limit of detection (LOD) was 0.023 µg/mL and the limit of quantification (LOQ) was 0.029 µg/mL. Average recoveries from spiked urine samples ranged from 96–100% and the coefficient of variation was less than 4% for both intra and inter-day assays with excellent repeatability. With the comparison with an ELISA kit, the developed kit is highly sensitive, performs well over the detection range, provides repeatable results in a short time, and can easily be used at point-of-care (POC), making it an ideal candidate for rapid testing in early detection, community screening for renal function disorders. View Full-Text
Keywords: quantitative fluorescent immunoassay; non-invasive lateral flow assay; Point-of-Care Cystatin-C test quantitative fluorescent immunoassay; non-invasive lateral flow assay; Point-of-Care Cystatin-C test
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MDPI and ACS Style

Natarajan, S.; DeRosa, M.C.; Shah, M.I.; Jayaraj, J. Development and Evaluation of a Quantitative Fluorescent Lateral Flow Immunoassay for Cystatin-C, a Renal Dysfunction Biomarker. Sensors 2021, 21, 3178. https://doi.org/10.3390/s21093178

AMA Style

Natarajan S, DeRosa MC, Shah MI, Jayaraj J. Development and Evaluation of a Quantitative Fluorescent Lateral Flow Immunoassay for Cystatin-C, a Renal Dysfunction Biomarker. Sensors. 2021; 21(9):3178. https://doi.org/10.3390/s21093178

Chicago/Turabian Style

Natarajan, Satheesh; DeRosa, Maria C.; Shah, Malay I.; Jayaraj, Joseph. 2021. "Development and Evaluation of a Quantitative Fluorescent Lateral Flow Immunoassay for Cystatin-C, a Renal Dysfunction Biomarker" Sensors 21, no. 9: 3178. https://doi.org/10.3390/s21093178

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