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Special Issue "Aptamers and Applications"

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: 30 June 2019

Special Issue Editors

Guest Editor
Dr. Beate Strehlitz

Department Environmental and Biotechnology Centre, Helmholtz Centre for Environmental Research – UFZ, Permoserstr. 15, 04318 Leipzig, Germany
Website | E-Mail
Phone: +49 341 2351764
Fax: +49 341 2351764
Interests: aptamer selection and characterization; SELEX; aptamer based sensors and assays; biosensor development and application; bioelectrotechnology; bioelectrochemistry
Guest Editor
Dr. Regina Stoltenburg

Department of Soil Ecology, Helmholtz Centre for Environmental Research – UFZ, Theodor-Lieser-Str. 4, 06120 Halle, Germany
Website | E-Mail
Interests: aptamer selection and application; SELEX; aptamer-based assays; aptasensors; ELONA; SPR; G-quadruplexes; genomics; microbiology; microbial communities; yeasts

Special Issue Information

Dear Colleagues,

For more than 25 years ago, aptamers have been a very attractive class of targeting molecules and have received growing interest in diverse scientific fields. Aptamers are known as a special kind of functional nucleic acids. Different from the conventional view on nucleic acids as carriers of genetic information, aptamers are more like globular molecules and their functionality is based on their complex three-dimensional structure. The intramolecular folding in accordance with the primary sequence of the aptamers enables them to recognize and bind their targets with high affinity and specificity. Aptamers are generated by an evolutionary approach called SELEX that leads to the enrichment of specific target-binding aptamers from a highly diverse oligonucleotide library during iterative selection cycles. This technology has been extensively modified and optimized over the years and has thus become a powerful method for the selection of high-affinity aptamers for a variety of target molecules (from small molecular targets over proteins to complex target structures).

Aptamers are currently heading towards applications ranging from basic research, medicine and pharmacy to environmental, water, or food analytics. They have great potential as therapeutic or diagnostic agents, delivery agents, molecular imaging tools, and as capture or reporter molecules. Aptamers have been successfully verified as molecular recognition elements in a wide range of analytical systems including differently designed aptamer-based detection assays (e.g., colorimetric or fluorescence-based assays, SPR, ELONA, LFA and others) or aptasensors (with electrochemical, optical, acoustic or other detection principles). To date, the generation of high-quality aptamers remains a complex process and the comprehensive transition of aptamers from research to real applications is a major challenge. This Special Issue of Sensors will cover current research topics in the exciting field of aptamers and how they are trained for application.

We welcome submissions that expand the world of aptamers by describing new aptamer developments and methods thereof, and highlight beneficial features of aptamers by showing examples of new applications. Both research papers and review articles will be considered. We look forward to and welcome your participation in this Special Issue.

Dr. Beate Strehlitz
Dr. Regina Stoltenburg
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • aptamers
  • nucleic acid
  • SELEX
  • aptamer selection
  • applications
  • aptasensors
  • aptamer-based assays
  • affinity molecules
  • specificity and sensitivity
  • aptamer–target interaction

Published Papers (2 papers)

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Research

Open AccessArticle
An Aptamer-Based Biosensor for Direct, Label-Free Detection of Melamine in Raw Milk
Sensors 2018, 18(10), 3227; https://doi.org/10.3390/s18103227
Received: 23 August 2018 / Revised: 19 September 2018 / Accepted: 20 September 2018 / Published: 25 September 2018
Cited by 1 | PDF Full-text (2078 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Melamine, a nitrogen-rich compound, has been used as a food and milk additive to falsely increase the protein content. However, melamine is toxic, and high melamine levels in food or in milk can cause kidney and urinary problems, or even death. Hence, the [...] Read more.
Melamine, a nitrogen-rich compound, has been used as a food and milk additive to falsely increase the protein content. However, melamine is toxic, and high melamine levels in food or in milk can cause kidney and urinary problems, or even death. Hence, the detection of melamine in food and milk is desirable, for which numerous detection methods have been developed. Several methods have successfully detected melamine in raw milk; however, they require a sample preparation before the analyses. This study aimed to develop an aptamer-DNAzyme conjugated biosensor for label-free detection of melamine, in raw milk, without any sample preparation. An aptamer-DNAzyme conjugated biosensor was developed via screening using microarray analysis to identify the candidate aptamers followed by an optimization, to reduce the background noise and improve the aptamer properties, thereby, enhancing the signal-to-noise (S/N) ratio of the screened biosensor. The developed biosensor was evaluated via colorimetric detection and tested with raw milk without any sample preparation, using N-methylmesoporphyrin IX for fluorescence detection. The biosensor displayed significantly higher signal intensity at 2 mM melamine (S/N ratio, 20.2), which was sufficient to detect melamine at high concentrations, in raw milk. Full article
(This article belongs to the Special Issue Aptamers and Applications)
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Open AccessArticle
A Label-Free Aptasensor for Ochratoxin a Detection Based on the Structure Switch of Aptamer
Sensors 2018, 18(6), 1769; https://doi.org/10.3390/s18061769
Received: 20 April 2018 / Revised: 28 May 2018 / Accepted: 28 May 2018 / Published: 1 June 2018
Cited by 3 | PDF Full-text (3110 KB) | HTML Full-text | XML Full-text
Abstract
A label-free sensing platform is developed based on switching the structure of aptamer for highly sensitive and selective fluorescence detection of ochratoxin A (OTA). OTA induces the structure of aptamer, transforms into G-quadruplex and produces strong fluorescence in the presence of zinc(II)-protoporphyrin IX [...] Read more.
A label-free sensing platform is developed based on switching the structure of aptamer for highly sensitive and selective fluorescence detection of ochratoxin A (OTA). OTA induces the structure of aptamer, transforms into G-quadruplex and produces strong fluorescence in the presence of zinc(II)-protoporphyrin IX probe due to the specific bind to G-quadruplex. The simple method exhibits high sensitivity towards OTA with a detection limit of 0.03 nM and excellent selectivity over other mycotoxins. In addition, the successful detection of OTA in real samples represents a promising application in food safety. Full article
(This article belongs to the Special Issue Aptamers and Applications)
Figures

Figure 1

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