Special Issue "Detection and Diagnostics of Fungal and Oomycete Plant Pathogens"

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Protection".

Deadline for manuscript submissions: 31 January 2020.

Special Issue Editor

Dr. Guillaume Bilodeau
E-Mail Website
Guest Editor
Canadian Food Inspection Agency, Ottawa Plant Laboratory, Ottawa, ON, K2J 4S1, Canada
Tel. 343-212-0283
Interests: technologies for detection and identification of plant pests (fungi-oomycetes) of regulatory significance; fungal detection and genotyping; phytophthora; Verticillium; real-time PCR; genomic; metagenomic

Special Issue Information

Dear Colleagues,

In agriculture and forestry the development and validation of molecular detection tools for different organisms is important for phytosanitary export certification, which relies on having methods to identify plant pathogens associated with plants, grains and seeds. To develop detection–identification assays, DNA sequence information and genomic resources are important. Over the last decade, several genomes of plant pathogens have been made available, largely due to the development of high-throughput sequencing (HTS) technologies that have enabled lower costs for sequencing genomes and the acquisition of data conducive to running metagenomic analyses. Knowledge of the genome and its structure can help us better understand these organisms and is a valuable resource for the development of detection and genotyping tools. Often disease may be present in plants despite no symptoms being visible. This underscores the importance of having molecular methods for the detection of pathogens for improved sensitivity and to allow high-throughput sample processing while decreasing the dependency on time-consuming culturing methods. The development of technologies for the detection and identification of pathogenic fungi and oomycetes is continuously evolving as new and innovative tools for sequencing and analyzing genomes become available. Advances in techniques such as qPCR, isothermal amplification, AmpliSeq technologies and portable tools that provide faster and easier detection capabilities will be presented in this Special Issue.

Dr. Guillaume Bilodeau
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • detection and identification of plant pests
  • fungi-oomycetes
  • molecular detection
  • molecular tools
  • HTS detection
  • genomics

Published Papers (1 paper)

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Research

Open AccessCommunication
Uncovering the Host Range for Maize Pathogen Magnaporthiopsis maydis
Plants 2019, 8(8), 259; https://doi.org/10.3390/plants8080259 - 30 Jul 2019
Abstract
The fungus Magnaporthiopsis maydis is a soil-borne, seed-borne vascular wilt pathogen that causes severe damage to sensitive Zea mays L. (maize) hybrids throughout Egypt, Israel, India, Spain, and other countries. It can undergo virulence variations and survive as spores, sclerotia, or mycelia on [...] Read more.
The fungus Magnaporthiopsis maydis is a soil-borne, seed-borne vascular wilt pathogen that causes severe damage to sensitive Zea mays L. (maize) hybrids throughout Egypt, Israel, India, Spain, and other countries. It can undergo virulence variations and survive as spores, sclerotia, or mycelia on plant residues. Maize, Lupinus termis L. (lupine) and Gossypium hirsutum L. (cotton) are the only known hosts of M. maydis. Identification of new plant hosts that can assist in the survival of the pathogen is an essential step in restricting disease outbreak and spread. Here, by field survey and growth chamber pathogenicity test, accompanied by real-time PCR analysis, the presence of the fungal DNA inside the roots of cotton (Pima cv.) plants was confirmed in infested soil. Moreover, we identified M. maydis in Setaria viridis (green foxtail) and Citrullus lanatus (watermelon, Malali cv.). Infected watermelon sprouts had delayed emergence and development, were shorter, and had reduced root and shoot biomass. M. maydis infection also affected root biomass and phenological development of cotton plants but caused only mild symptoms in green foxtail. No M. maydis DNA was detected in Hordeum vulgare (barley, Noga cv.) and the plants showed no disease symptoms except for reduced shoot weight. These findings are an important step towards uncovering the host range and endophytic behavior of M. maydis, encouraging expanding this evaluation to other plant species. Full article
(This article belongs to the Special Issue Detection and Diagnostics of Fungal and Oomycete Plant Pathogens)
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