Phytochemical Analysis, Antioxidant, Antimicrobial, and Cytotoxic Activity of Plant Extracts

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Phytochemistry".

Deadline for manuscript submissions: 20 July 2024 | Viewed by 1145

Special Issue Editors

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Guest Editor
1. Centre for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia
2. Institute of Public Health Kragujevac, 34000 Kragujevac, Serbia
Interests: cell biology; microbiology; immunology; cancer research

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Guest Editor
Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Serbia
Interests: phytochemistry; chromatographic analysis and isolation of secondary metabolites; antioxidant; antibacterial and cytotoxic activities of major and minor secondary metabolites presented in plant extract

Special Issue Information

Dear Colleagues,

According to the World Health Organization (WHO), as well as numerous scientific research, almost 80% of the world’s population relies on usage of medicinal plants in primary health care. The medicinal importance of these plants and their extracts are closely related to the presence of specific active secondary metabolites (alkaloids, phenolic compounds, flavonoids, terpenes, etc...). Many of these biomolecules can exhibit several pharmacological properties. Hence, herbal extracts often interfere with several animal organs, tissues, cells, and molecular targets, resulting in relieving (or reducing) symptoms of ailments or diseases.

The focusses of this Special Issue are on antioxidant, antimicrobial, and cytotoxic activity of plant extracts. We would like to invite researchers to submit its original scientific results (in the form of manuscripts, reviews, mini-reviews, and short communications) which cover phytochemistry, as well as appropriate pharmacological properties of plant extracts and their major and minor bioactive constituents.

Prof. Dr. Dejan D. Baskić
Prof. Dr. Nenad L. Vuković
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • plant extracts
  • bioactive secondary metabolites chromatographic analysis of major and minor constituents of plant extracts and essential oils
  • antioxidant activity of plant extracts and essential oils
  • antimicrobial activity of plant extracts and essential oils
  • cytotoxic activity of plant extracts and essential oils

Published Papers (1 paper)

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31 pages, 3126 KiB  
Citrus limon Essential Oil: Chemical Composition and Selected Biological Properties Focusing on the Antimicrobial (In Vitro, In Situ), Antibiofilm, Insecticidal Activity and Preservative Effect against Salmonella enterica Inoculated in Carrot
by Miroslava Kačániová, Natália Čmiková, Nenad L. Vukovic, Andrea Verešová, Alessandro Bianchi, Stefania Garzoli, Rania Ben Saad, Anis Ben Hsouna, Zhaojun Ban and Milena D. Vukic
Plants 2024, 13(4), 524; - 15 Feb 2024
Cited by 1 | Viewed by 899
New goals for industry and science have led to increased awareness of food safety and healthier living in the modern era. Here, one of the challenges in food quality assurance is the presence of pathogenic microorganisms. As planktonic cells can form biofilms and [...] Read more.
New goals for industry and science have led to increased awareness of food safety and healthier living in the modern era. Here, one of the challenges in food quality assurance is the presence of pathogenic microorganisms. As planktonic cells can form biofilms and go into a sessile state, microorganisms are now more resistant to broad-spectrum antibiotics. Due to their proven antibacterial properties, essential oils represent a potential option to prevent food spoilage in the search for effective natural preservatives. In this study, the chemical profile of Citrus limon essential oil (CLEO) was evaluated. GC-MS analysis revealed that limonene (60.7%), β-pinene (12.6%), and γ-terpinene (10.3%) are common constituents of CLEO, which prompted further research on antibacterial and antibiofilm properties. Minimum inhibitory concentration (MIC) values showed that CLEO generally exhibits acceptable antibacterial properties. In addition, in situ antimicrobial research revealed that vapour-phase CLEO can arrest the growth of Candida and Y. enterocolitica species on specific food models, indicating the potential of CLEO as a preservative. The antibiofilm properties of CLEO were evaluated by MIC assays, crystal violet assays, and MALDI-TOF MS analysis against S. enterica biofilm. The results of the MIC and crystal violet assays showed that CLEO has strong antibiofilm activity. In addition, the data obtained by MALDI-TOF MS investigation showed that CLEO altered the protein profiles of the bacteria studied on glass and stainless-steel surfaces. Our study also found a positive antimicrobial effect of CLEO against S. enterica. The anti-Salmonella activity of CLEO in vacuum-packed sous vide carrot samples was slightly stronger than in controls. These results highlight the advantages of the antibacterial and antibiofilm properties of CLEO, suggesting potential applications in food preservation. Full article
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