Phytochemical Investigations for Drug Discovery, Development and Biotechnological Approaches for Health and Agriculture

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Phytochemistry".

Deadline for manuscript submissions: 31 July 2025 | Viewed by 327

Special Issue Editors


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Guest Editor
Laboratory of Phytochemistry, Postgraduate Program in Biotechnology, Campus Centro-Oeste, Universidade Federal de São João del-Rei, Divinópolis 35501-296, MG, Brazil
Interests: natural products; phytochemistry; organic chemistry
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
Laboratory of Pharmacobotany and Medicinal Plants, Postgraduate Program in Biotechnology, Campus Centro-Oeste, Universidade Federal de São João del-Rei, Divinópolis 35501-296, MG, Brazil
Interests: plant tissue culture; secondary metabolites; plant biotechnology; plant physiology; medicinal plants
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
Biotechnological Processes Laboratory, Centro-Oeste Campus, Federal University of São João del-Rei, Divinópolis 35501-296, MG, Brazil
Interests: nanomaterials; environmental and biological applications; nanoremediators

Special Issue Information

Dear Colleagues,

Plants are a source of new substances that can have several biological activities, such as antioxidant, anti-inflammatory, antimicrobial, larvicidal, and allelopathic, activities, among others. They can also provide material to be transformed by other organisms into useful substances to humans such as propolis. Knowledge about the secondary metabolites of plants is important for understanding their biological and pharmacological activities. The use of biotechnology in this context is a powerful tool that assists in research and sustainable development for the discovery of new drugs and other compounds that can be applied to benefit health and agriculture fields. This Special Issue of Plants will highlight the application of biotechnological approaches that can contribute to the discovery, development and biological testing of phytochemicals for use as new drugs for human health or for agriculture as phytohormones, natural herbicides and pesticides.

This Special Issue aims to present an overview of progress and future trends in areas such as biotechnology, phytochemistry, biochemistry, pharmacology and plant physiology associated with the application of the plant metabolites in human health and agriculture, providing an update on new phytochemicals and their uses.

Dr. Luciana Alves Rodrigues Dos Santos Lima
Dr. Ana Hortência Fonseca Castro
Dr. Fernanda Maria Policarpo Tonelli
Guest Editors

Manuscript Submission Information

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Keywords

  • plant science
  • in vitro culture
  • plant biotechnology
  • phytochemistry
  • bioactive metabolites
  • biological activities
  • allelochemicals
  • natural herbicides
  • phytohormones

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Published Papers (1 paper)

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Research

20 pages, 3721 KiB  
Article
In Vitro Production of Smilax brasiliensis Seedlings, Callus Induction, Chemical Profile, and Assessment of Antioxidant Activity
by Paula Avelar Amado, Ana Hortência Fonsêca Castro, Lucas Santos Azevedo, Mariana Guerra de Aguilar, Lúcia Pinheiro Santos Pimenta and Luciana Alves Rodrigues dos Santos Lima
Plants 2025, 14(9), 1383; https://doi.org/10.3390/plants14091383 - 3 May 2025
Viewed by 119
Abstract
This study aimed to assess the production of Smilax brasiliensis seedlings in an in vitro environment and their adaptation to natural conditions, as well as the callus induction, the chemical profile of calli extracts, and their antioxidant potential. The seedlings were obtained from [...] Read more.
This study aimed to assess the production of Smilax brasiliensis seedlings in an in vitro environment and their adaptation to natural conditions, as well as the callus induction, the chemical profile of calli extracts, and their antioxidant potential. The seedlings were obtained from S. brasiliensis seeds germinated in Murashige and Skoog (MS) medium. The germination rate was 33%, and about 22% of the seeds produced whole seedlings. Three-month-old seedlings were acclimatized for two months, resulting in an 80% survival rate and improved physiological characteristics. Callus induction was initiated from leaf explants obtained from seedlings and plant growth regulators (PGRs), with and without light exposure. Calli extracts were obtained using methanol; phenolic compound and flavonoid quantification were performed, and the chemical profile was determined by nuclear magnetic resonance (1H NMR). For comparison, methanol extract from S. brasiliensis leaves collected in Brazilian Cerrado were also analyzed. Antioxidant activity was assessed using the 2,2-diphenyl-1-picryl-hydrazyl method and the ferric-reducing antioxidant power assay. All samples exhibited antioxidant activity according to the methods employed. Furthermore, 1H NMR revealed metabolic profile changes in the calli extracts compared to the leaf extract. This study yielded promising results, suggesting that in vitro culture could improve productivity and conserve the species, although changes were observed in the metabolic profile of S. brasiliensis. Full article
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