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In Vitro Techniques on Plant Propagation and Genetic Improvement, 2nd...
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In Vitro Techniques on Plant Propagation and Genetic Improvement, 2nd Edition

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: closed (20 January 2026) | Viewed by 4217

Special Issue Editors

Dr. Hasan Mehraj

E-Mail Website
Guest Editor
Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada-ku, Kobe 657-8501, Japan
Interests: heterosis; epigenetics; genotype to phenotype; brassica; plant tissue culture
Special Issues, Collections and Topics in MDPI journals
Dr. Monika Tuleja

E-Mail Website
Guest Editor
Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University, Gronostajowa 9, 30-387 Kraków, Poland
Interests: in vitro plant cultures; aspects of in vitro morphogenesis of selected species; including apomictic species; stress in vitro; the role of ethylene and other regulators of plant growth and development
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In vitro propagation is widely applied in various plants. It is a suitable way to ensure the genetic conservation of any endangered plant species or any other plant genetic resources. In vitro propagation in plants is highly species-specific and has been influenced by various factors such as carbon sources, plant growth regulators, light quality, and light intensity. Techniques such as callus culture, protoplast culture, organogenesis, and regeneration of protocorms like body (PLB), anther culture, ovary culture, and somatic embryogenesis are commonly used for in vitro plant propagation. In vitro propagation has also been extensively used for plant improvements through the application of embryo rescue, protoplast fusion, in vitro fertilization, and ploidy manipulation. Additionally, it has been used for genetic transformation and bioactive compound or secondary metabolite production.

We aim to accumulate current updates on in vitro techniques in plants in this Special Issue. Therefore, we welcome manuscripts on in vitro plant propagation, crop improvement, bioactive compounds, genetic conservation, genetic and genomic studies, epigenetic and epigenomic studies, transcriptomics, etc.

Dr. Hasan Mehraj
Dr. Monika Tuleja
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • callus
  • somatic embryogenesis
  • PLB
  • plant growth regulators
  • light
  • morphogenetic response
  • secondary metabolites
  • stress reaction
  • plant improvement
  • transformation
  • DNA markers
  • genetics and genomics
  • epigenetics and epigenomics
  • transcriptomics

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Published Papers (2 papers)

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Research

15 pages, 2781 KB  
Article
Direct Organogenesis of Epipremnum aureum G.S. Bunting for Mass Propagation
by Hai T. Nguyen, Quyet V. Khuat, Thao T. Ninh, Anh T. P. Dang, Le T. Nguyen, Elena A. Kalasnıkova, Abdulmalik A. Batukaev and Rima N. Kirakosyan
Plants 2025, 14(21), 3299; https://doi.org/10.3390/plants14213299 - 29 Oct 2025
Cited by 1 | Viewed by 1411
Abstract
Pothos (Epipremnum aureum G.S. Bunting), which belongs to the Arum family (Araceae Juss.), can be used for medicinal, ornamental, and pollutant-purifying purposes. Due to the usefulness of pothos, the market demand for this species is increasing. Our study attempts to fill in [...] Read more.
Pothos (Epipremnum aureum G.S. Bunting), which belongs to the Arum family (Araceae Juss.), can be used for medicinal, ornamental, and pollutant-purifying purposes. Due to the usefulness of pothos, the market demand for this species is increasing. Our study attempts to fill in the shortcomings of previous studies on the effect of activated carbon and plant growth regulators on the ability of shoots to take root in vitro, as well as the effect of inexpensive and readily available materials on the transition of seedlings from in vitro to the greenhouse stage. To evaluate the shooting results, Murashige and Skoog medium (MS) was used, which included 6-benzylaminopurine (BA), kinetin (Kn), α-naphthaleneacetic acid (α-NAA), coconut water, activated carbon, and indole-3-butyric acid (IBA) in various concentrations and combinations. Our results showed that the MS medium with the addition of 2.5 mg/L BA and 1.0 mg/L Kn was optimal for propagation by shoots. In this variant, 2.86 shoots per explant, 1.87 cm of shoot length, and 1.59 leaves per shoot were obtained. Despite the fact that this treatment provided the highest total cytokinin concentration, it was significantly more effective than only BA (2.5 mg/L) and all combinations of BA+α-NAA or Kn+α-NAA. For rooting, the micro shoots obtained on the above medium were transferred to MS + 0.25 mg/L α-NAA + 0.5 g/L AC, which allowed for rooting by 93.33%, 1.93 roots per explant, and root lengths by 2.37 cm. This is higher than with the IBA-based treatment, which led to a shortening of the roots and a reduction in their branching. Acclimatization in a 1:1 mixture (by volume) of loamy garden soil (pH 6.2, 2.1% organic matter) and coconut coir (particle size 0.5–2 mm) gave 75% survival after 40 days. These results have opened up the prospect of developing an effective method for reproducing pothos species in vitro by organogenesis at the lowest cost. Full article
(This article belongs to the Special Issue In Vitro Techniques on Plant Propagation and Genetic Improvement, 2nd Edition)
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15 pages, 3367 KB  
Article
Chitosan Nanoparticles: An Alternative for In Vitro Multiplication of Sugarcane (Saccharum spp.) in Semi-Automated Bioreactors
by Eucario Mancilla-Álvarez, María Karen Serrano-Fuentes, María Angélica Fuentes-Torres, Ricardo Sánchez-Páez and Jericó Jabín Bello-Bello
Plants 2025, 14(11), 1697; https://doi.org/10.3390/plants14111697 - 1 Jun 2025
Cited by 8 | Viewed by 2242
Abstract
Chitosan nanoparticles (CsNPs) are biocompatible, biodegradable, and non-toxic natural polymers at low concentrations with diverse applications in in vitro plant tissue culture. This study aims to evaluate the effect of CsNPs during in vitro multiplication of sugarcane (Saccharum spp.) using temporary immersion [...] Read more.
Chitosan nanoparticles (CsNPs) are biocompatible, biodegradable, and non-toxic natural polymers at low concentrations with diverse applications in in vitro plant tissue culture. This study aims to evaluate the effect of CsNPs during in vitro multiplication of sugarcane (Saccharum spp.) using temporary immersion bioreactors. CsNPs were evaluated at concentrations of 0, 25, 50, 100, and 200 mg L−1 in Murashige and Skoog liquid culture medium. After four weeks of culture, response percentage, the number of shoots per explant, shoot length, number of leaves per explant, dry matter, chlorophyll content, β-carotene content, lipid peroxidation, phenolic content, hydrogen peroxide content, and antioxidant capacity were evaluated. The results showed that the highest response percentages were obtained in the treatments with 0, 25, and 50 mg L−1 CsNPs, whereas the lowest response percentages were obtained in the treatments with 100 and 200 mg L−1 CsNPs. Concentrations of 25 and 50 mg L−1 CsNPs promoted cell growth and differentiation, whereas 100 and 200 mg L−1 CsNPs inhibited it. Chlorophyll content increased by 25 and 50 mg L-1 CsNPs, whereas β-carotene content increased by 100 and 200 mg L−1 CsNPs. Lipid peroxidation and antioxidant capacity increased with increasing CsNP concentrations. The phenolic content increased by 100 mg L−1 CsNPs, whereas the hydrogen peroxide content decreased with increasing CsNP concentrations. In conclusion, CsNPs are an alternative for stimulating tissue growth and differentiation during the in vitro multiplication of sugarcane. Full article
(This article belongs to the Special Issue In Vitro Techniques on Plant Propagation and Genetic Improvement, 2nd Edition)
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