Research

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16 pages, 3735 KiB

Open AccessArticle

Docetaxel-Loaded Methoxy poly(ethylene glycol)-poly (L-lactic Acid) Nanoparticles for Breast Cancer: Synthesis, Characterization, Method Validation, and Cytotoxicity

by Shumaila Miraj, Hamid Saeed, Mehwish Iqtedar, Norah A. Albekairi, Nadeem Ahmed, Muhammad Zeeshan Danish, Muhammad Islam, Muhammad Fawad Rasool, Kashif Mairaj Deen and Hassaan Anwer Rathore

Pharmaceuticals 2023, 16(11), 1600; https://doi.org/10.3390/ph16111600 - 13 Nov 2023

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Abstract

This study aimed to synthesize and characterize DTX-mPEG-PLA-NPs along with the development and validation of a simple, accurate, and reproducible method for the determination and quantification of DTX in mPEG-PLA-NPs. The prepared NPs were characterized using AFM, DLS, zetasizer, and drug release kinetic [...] Read more.

This study aimed to synthesize and characterize DTX-mPEG-PLA-NPs along with the development and validation of a simple, accurate, and reproducible method for the determination and quantification of DTX in mPEG-PLA-NPs. The prepared NPs were characterized using AFM, DLS, zetasizer, and drug release kinetic profiling. The RP-HPLC assay was developed for DTX detection. The cytotoxicity and anti-clonogenic effects were estimated using MTT and clonogenic assays, respectively, using both MCF-7 and MDA-MB-231 cell lines in a 2D and 3D culture system. The developed method showed a linear response, high precision, accuracy, RSD values of ≤2%, and a tailing factor ≤2, per ICH guidelines. The DTX-mPEG-PLA-NPs exhibited an average particle size of 264.3 nm with an encapsulation efficiency of 62.22%. The in vitro drug kinetic profile, as per the Krosmeyers–Peppas model, demonstrated Fickian diffusion, with initial biphasic release and a multistep sustained release over 190 h. The MTT assay revealed improved in vitro cytotoxicity against MCF-7 and MDA-MB-231 in the 2D cultures and MCF-7 3D mammosphere cultures. Significant inhibitions of the clonogenic potential of MDA-MB-231 were observed for all concentrations of DTX-mPEG-PLA-NPs. Our results highlight the feasibility of detecting DTX via the robust RP-HPLC method and using DTX-mPEG-PLA-NPs as a perceptible and biocompatible delivery vehicle with greater cytotoxic and anti-clonogenic potential, supporting improved outcomes in BC. Full article

(This article belongs to the Special Issue Biodegradable Polymeric Nanoparticles for Drug Delivery)

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18 pages, 2524 KiB

Open AccessArticle

Preparation of Selenium-Based Drug-Modified Polymeric Ligand-Functionalised Fe₃O₄ Nanoparticles as Multimodal Drug Carrier and Magnetic Hyperthermia Inductor

by Itziar Galarreta-Rodriguez, Mikel Etxebeste-Mitxeltorena, Esther Moreno, Daniel Plano, Carmen Sanmartín, Saad Megahed, Neus Feliu, Wolfgang J. Parak, Eneko Garaio, Izaskun Gil de Muro, Luis Lezama, Idoia Ruiz de Larramendi and Maite Insausti

Pharmaceuticals 2023, 16(7), 949; https://doi.org/10.3390/ph16070949 - 30 Jun 2023

Cited by 2 | Viewed by 1334

Abstract

In recent years, much effort has been invested into developing multifunctional drug delivery systems to overcome the drawbacks of conventional carriers. Magnetic nanoparticles are not generally used as carriers but can be functionalised with several different biomolecules and their size can be tailored [...] Read more.

In recent years, much effort has been invested into developing multifunctional drug delivery systems to overcome the drawbacks of conventional carriers. Magnetic nanoparticles are not generally used as carriers but can be functionalised with several different biomolecules and their size can be tailored to present a hyperthermia response, allowing for the design of multifunctional systems which can be active in therapies. In this work, we have designed a drug carrier nanosystem based on Fe₃O₄ nanoparticles with large heating power and 4-amino-2-pentylselenoquinazoline as an attached drug that exhibits oxidative properties and high selectivity against a variety of cancer malignant cells. For this propose, two samples composed of homogeneous Fe₃O₄ nanoparticles (NPs) with different sizes, shapes, and magnetic properties have been synthesised and characterised. The surface modification of the prepared Fe₃O₄ nanoparticles has been developed using copolymers composed of poly(ethylene-alt-maleic anhydride), dodecylamine, polyethylene glycol and the drug 4-amino-2-pentylselenoquinazoline. The obtained nanosystems were properly characterised. Their in vitro efficacy in colon cancer cells and as magnetic hyperthermia inductors was analysed, thereby leaving the door open for their potential application as multimodal agents. Full article

(This article belongs to the Special Issue Biodegradable Polymeric Nanoparticles for Drug Delivery)

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Review

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15 pages, 2391 KiB

Open AccessReview

Asymmetric Lipid Vesicles: Techniques, Applications, and Future Perspectives as an Innovative Drug Delivery System

by Denisse Gardea-Gutiérrez, Eduardo Núñez-García, Berenice E. Oseguera-Guerra, Manuel Román-Aguirre and Silvia L. Montes-Fonseca

Pharmaceuticals 2023, 16(6), 777; https://doi.org/10.3390/ph16060777 - 23 May 2023

Cited by 4 | Viewed by 2106

Abstract

Novel lipid-based nanosystems have been of interest in improving conventional drug release methods. Liposomes are the most studied nanostructures, consisting of lipid bilayers ideal for drug delivery, thanks to their resemblance to the cell plasma membrane. Asymmetric liposomes are vesicles with different lipids [...] Read more.

Novel lipid-based nanosystems have been of interest in improving conventional drug release methods. Liposomes are the most studied nanostructures, consisting of lipid bilayers ideal for drug delivery, thanks to their resemblance to the cell plasma membrane. Asymmetric liposomes are vesicles with different lipids in their inner and outer layers; because of this, they can be configured to be compatible with the therapeutic drug while achieving biocompatibility and stability. Throughout this review, topics such as the applications, advantages, and synthesis techniques of asymmetric liposomes will be discussed. Further, an in silico analysis by computational tools will be examined as a helpful tool for designing and understanding asymmetric liposome mechanisms in pharmaceutical applications. The dual-engineered design of asymmetric liposomes makes them an ideal alternative for transdermal drug delivery because of the improved protection of pharmaceuticals without lowering adsorption rates and system biocompatibility. Full article

(This article belongs to the Special Issue Biodegradable Polymeric Nanoparticles for Drug Delivery)

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