Special Issue "Deciphering Viral-Host Interactions with Single Molecule and Superresolution Imaging Approaches"

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Human Pathogens".

Deadline for manuscript submissions: 1 May 2021.

Special Issue Editor

Dr. Schuyler Van Engelenburg

Guest Editor
Department of Biological Sciences, University of Denver, 80210 Denver, CO, USA
Interests: superresolution imaging; single molecule imaging approaches; Human Immunodeficiency Virus-1; lentiviruses; virus assembly; host–virus interactions

Special Issue Information

Dear Colleagues,

The overall focus of this Special Issue will be on leveraging single-molecule and superresolution imaging approaches to understand viral pathogenesis.

The scope of the submitted articles will be sufficiently broad to encompass research investigating (but not limited to) virus attachment/entry, virus trafficking, immune evasion/function, viral replication, and virus assembly/release utilizing single virus/molecule and superresolution imaging approaches.

The purpose of the Special Issue is to increase awareness of the power of modern single molecule/superresolution imaging approaches to answer fundamental question regarding viral pathogenesis.

We look forward to your valuable contributions that will promote further developments in this field.

Thank you for your collaboration.

Dr. Schuyler Van Engelenburg
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Pathogens is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1500 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • superresolution imaging
  • single molecule imaging
  • biophysics
  • virus entry
  • virus replication
  • immune evasion
  • virus trafficking
  • virus assembly

Published Papers (1 paper)

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Research

Open AccessArticle
A Quantitative Live-Cell Superresolution Imaging Framework for Measuring the Mobility of Single Molecules at Sites of Virus Assembly
Pathogens 2020, 9(11), 972; https://doi.org/10.3390/pathogens9110972 - 21 Nov 2020
Abstract
The insurgence of superresolution microscopy into the fields of virology and microbiology has begun to enable the mapping of molecular assemblies critical for host–pathogen interfaces that organize on a scale below the resolution limit of the light microscope. It is, however, challenging to [...] Read more.
The insurgence of superresolution microscopy into the fields of virology and microbiology has begun to enable the mapping of molecular assemblies critical for host–pathogen interfaces that organize on a scale below the resolution limit of the light microscope. It is, however, challenging to completely understand the molecular interactions between host and pathogen from strictly time-invariant observations. Herein, we describe a method using simultaneous dual-color superresolution microscopy to gain both structural and dynamic information about HIV-1 assembly. Specifically, we demonstrate the reconstruction of single virus assembly sites using live-cell photo-activated localization microscopy (PALM) while concurrently assessing the sub-viral mobility of the HIV-1 envelope glycoprotein during interaction with the viral lattice. We propose that our method is broadly applicable to elucidating pathogen and host protein–protein interactions through quantification of the dynamics of these proteins at the nanoscale. Full article
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