Feature Papers in Methods and Protocols 2026

A special issue of Methods and Protocols (ISSN 2409-9279).

Deadline for manuscript submissions: 31 December 2026 | Viewed by 1007

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1. School of Chemistry and Physics, University of KwaZulu-Natal, Durban, South Africa
2. Department of Organic Chemistry, University of Barcelona, Barcelona, Spain
Interests: antimicrobial peptides; solid-phase chemistry; combinatorial chemistry; drug delivery systems; peptide drug conjugates; orthogonal chemistry; drug discovery; biomaterials
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Proteomics and Metabolomics Facility, Center for Biotechnology, Institute of Agriculture and Natural Resources, University of Nebraska-Lincoln, N300, Beadle Center, 1901 Vine St, Lincoln, NE 68588-0665, USA
Interests: proteomics; mass spectrometry; post-translational modifications; phosphoproteomics; redox proteomics; targeted metabolomics; liquid chromatography; electrophoresis
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Special Issue Information

Dear Colleagues,

We are pleased to announce this Special Issue of Methods and Protocols, entitled “Feature Papers in Methods and Protocols 2026”. In this Special Issue, we will collate articles from top researchers describing new approaches or new cutting-edge developments to tackle questions in the fields of life sciences, chemistry, and biomedical sciences, including analytical chemistry and advanced measurement science. In particular, we encourage submissions focused on the emerging fields of “Omics and High Throughput”, “Public Health Research”, “Synthetic and Systems Biology”, and “Tissue Engineering and Organoids”, as well as innovative developments in sample preparation, elemental and molecular analysis, and spectroscopic and spectrometric techniques.

Our aim is to encourage scientists to publish new protocols based on established techniques, as well as significant improvements and developments of cutting-edge methods, with particular interest in methodological optimization, control of contamination and matrix effects, and validation of analytical performance. For all articles, background information necessary to understand the underlying principles, full experimental details, and comparison with available protocols/methods must be provided, including critical steps in sample handling and preanalytical variables when relevant. Scientists are encouraged to provide accompanying video demonstrations, which will be published online, for ease of reproducibility.

You are welcome to send short proposals for your feature paper to our Managing Editor Ms. Eunice Fu (eunice.fu@mdpi.com) or the Editorial Office (mps@mdpi.com) before submission. All papers will be subjected to a thorough and rigorous peer-review process.

We look forward to receiving your excellent work.

Prof. Dr. Fernando Albericio
Dr. Sophie Alvarez
Dr. Maria Luisa Astolfi
Guest Editors

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Keywords

  • cell biology
  • biochemistry
  • chemistry
  • nanotechnology
  • biotechnology
  • imaging
  • applied computation and bioinformatics
  • genomics
  • proteomics
  • developmental biology
  • model organisms
  • pharmacology
  • structural biology/chemistry
  • biophysics
  • tissue engineering
  • public health

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Published Papers (1 paper)

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14 pages, 10220 KB  
Protocol
Collection and Lipidomic Analysis of Murine Knee Synovium and Infrapatellar Fat Pad
by Tong Yang, Luke Stasikelis and Alexander J. Knights
Methods Protoc. 2026, 9(3), 70; https://doi.org/10.3390/mps9030070 - 2 May 2026
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Abstract
Intra-articular soft connective tissues such as synovium and adipose tissue play a crucial role in governing joint homeostasis and disease progression in various forms of arthritis. In the knee, like many synovial joints, adipose tissue forms an integrated anatomic and functional unit with [...] Read more.
Intra-articular soft connective tissues such as synovium and adipose tissue play a crucial role in governing joint homeostasis and disease progression in various forms of arthritis. In the knee, like many synovial joints, adipose tissue forms an integrated anatomic and functional unit with the joint-lining synovium, and the most prominent adipose depot is the infrapatellar fat pad (IFP). With growing evidence that lipid profiles in the synovium–IFP unit shift during progression of joint diseases like osteoarthritis (OA), there is strong impetus for consistent tissue collection approaches and reproducible subsequent lipid characterization. Here, we present a standardized dissection and low-input untargeted lipidomics workflow optimized for mouse knee synovium and IFP, to enable comprehensive lipid profiling. Synovium/IFP from multiple joints are pooled to increase input mass and guarantee robust lipid yield, followed by lipid extraction and high-resolution liquid chromatography-mass spectrometry (LC–MS) acquisition for global, untargeted lipidomic profiling. The analysis workflow encompasses robust feature detection, accurate lipid annotation, data transformation and normalization. These steps enhance comparability across samples, particularly those with low input amounts, while minimizing technical variance and batch effects. Using this approach, we detect a broad spectrum of lipid species spanning the major lipid categories. As expected for untargeted discovery, a subset of non-lipid species is also observed. This protocol provides a practical framework for robust, reproducible lipidomics in murine intra-articular soft tissues to support future disease-specific biomarker and drug target discovery in OA and other joint diseases. Full article
(This article belongs to the Special Issue Feature Papers in Methods and Protocols 2026)
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