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Most Cited

  • Article
  • Open Access
31 Citations
8,613 Views
10 Pages

Test−Retest Reliability of Isokinetic Ankle, Knee and Hip Strength in Physically Active Adults Using Biodex System 4 Pro

  • Juho Tuominen,
  • Mari Leppänen,
  • Heidi Jarske,
  • Kati Pasanen,
  • Tommi Vasankari and
  • Jari Parkkari

Background: The isokinetic dynamometry is considered a gold standard in muscle strength testing. The reliability of lower limb isokinetic strength measurements has not been thoroughly evaluated. Objective: To examine the test−retest reliability...

  • Article
  • Open Access
30 Citations
8,020 Views
12 Pages

Recent advances in genomic technologies have enabled more in-depth study of the oral microbiome. In this study, we compared the amplicons generated by primers targeting different sites of the 16S rRNA gene found in the Human Oral Microbiome Database...

  • Protocol
  • Open Access
23 Citations
7,765 Views
11 Pages

Real-Time Monitoring of Lysosomal Membrane Permeabilization Using Acridine Orange

  • Ida Eriksson,
  • Linda Vainikka,
  • Hans Lennart Persson and
  • Karin Öllinger

Loss of lysosomal membrane integrity results in leakage of lysosomal hydrolases to the cytosol which might harm cell function and induce cell death. Destabilization of lysosomes often precede apoptotic or necrotic cell death and occur during both phy...

  • Brief Report
  • Open Access
23 Citations
9,990 Views
10 Pages

Comparison of Automated and Traditional Western Blotting Methods

  • Aino Sormunen,
  • Emma Koivulehto,
  • Kari Alitalo,
  • Kalle Saksela,
  • Nihay Laham-Karam and
  • Seppo Ylä-Herttuala

Traditional Western blotting is one of the most used analytical techniques in biological research. However, it can be time-consuming and suffer from a lack of reproducibility. Consequently, devices with different degrees of automation have been devel...

  • Review
  • Open Access
20 Citations
4,130 Views
15 Pages

The detection of toxic heavy metal ions, especially cadmium (Cd), lead (Pb), zinc (Zn), and copper (Cu), is a global problem due to ongoing pollution incidents and continuous anthropogenic and industrial activities. Therefore, it is important to deve...

  • Protocol
  • Open Access
17 Citations
10,108 Views
17 Pages

Ion Selective Electrode (ISE) Method for Determination of Total Fluorine and Total Organic Fluorine in Packaging Substrates

  • Ma Cristine Concepcion D. Ignacio,
  • Greg W. Curtzwiler,
  • Mark R. Early,
  • Katie M. Updegraff and
  • Keith L. Vorst

18 January 2023

Various testing methods and techniques have been used to identify and quantify per- and polyfluoroalkyl substances (PFAS) in food packaging. A common indirect measurement of PFAS is total fluorine (TF) and total organic fluorine (TOF). These methods...

  • Commentary
  • Open Access
15 Citations
72,471 Views
8 Pages

DNA impurities can impact the safety of genetically engineered pharmaceuticals; thus, a specific limit value must be set for them during marketing authorisation. This particularly applies to mRNA vaccines, as large quantities of DNA templates are use...

  • Article
  • Open Access
14 Citations
4,773 Views
16 Pages

23 February 2023

Trauma triggers critical molecular and cellular signaling cascades that drive biological outcomes and recovery. Variations in the gene expression of common endogenous reference housekeeping genes (HKGs) used in data normalization differ between tissu...

  • Article
  • Open Access
13 Citations
3,807 Views
13 Pages

Ultrasound-Assisted Anthocyanins Extraction from Pigmented Corn: Optimization Using Response Surface Methodology

  • Annisa Nurkhasanah,
  • Titouan Fardad,
  • Ceferino Carrera,
  • Widiastuti Setyaningsih and
  • Miguel Palma

This study aimed to determine the optimal UAE conditions for extracting anthocyanins from pigmented corn using the Box–Behnken design (BBD). Six anthocyanins were identified in the samples and were used as response variables to evaluate the eff...

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Methods Protoc. - ISSN 2409-9279