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Most Cited

  • Article
  • Open Access
37 Citations
9,571 Views
10 Pages

Test−Retest Reliability of Isokinetic Ankle, Knee and Hip Strength in Physically Active Adults Using Biodex System 4 Pro

  • Juho Tuominen,
  • Mari Leppänen,
  • Heidi Jarske,
  • Kati Pasanen,
  • Tommi Vasankari and
  • Jari Parkkari

Background: The isokinetic dynamometry is considered a gold standard in muscle strength testing. The reliability of lower limb isokinetic strength measurements has not been thoroughly evaluated. Objective: To examine the test−retest reliability...

  • Article
  • Open Access
32 Citations
8,655 Views
12 Pages

Recent advances in genomic technologies have enabled more in-depth study of the oral microbiome. In this study, we compared the amplicons generated by primers targeting different sites of the 16S rRNA gene found in the Human Oral Microbiome Database...

  • Protocol
  • Open Access
26 Citations
8,528 Views
11 Pages

Real-Time Monitoring of Lysosomal Membrane Permeabilization Using Acridine Orange

  • Ida Eriksson,
  • Linda Vainikka,
  • Hans Lennart Persson and
  • Karin Öllinger

Loss of lysosomal membrane integrity results in leakage of lysosomal hydrolases to the cytosol which might harm cell function and induce cell death. Destabilization of lysosomes often precede apoptotic or necrotic cell death and occur during both phy...

  • Brief Report
  • Open Access
26 Citations
10,566 Views
10 Pages

Comparison of Automated and Traditional Western Blotting Methods

  • Aino Sormunen,
  • Emma Koivulehto,
  • Kari Alitalo,
  • Kalle Saksela,
  • Nihay Laham-Karam and
  • Seppo Ylä-Herttuala

Traditional Western blotting is one of the most used analytical techniques in biological research. However, it can be time-consuming and suffer from a lack of reproducibility. Consequently, devices with different degrees of automation have been devel...

  • Review
  • Open Access
22 Citations
4,546 Views
15 Pages

The detection of toxic heavy metal ions, especially cadmium (Cd), lead (Pb), zinc (Zn), and copper (Cu), is a global problem due to ongoing pollution incidents and continuous anthropogenic and industrial activities. Therefore, it is important to deve...

  • Protocol
  • Open Access
19 Citations
7,132 Views
7 Pages

A Robust and Standardized Approach to Quantify Wound Closure Using the Scratch Assay

  • Stefan Balko,
  • Evan Kerr,
  • Edward Buchel,
  • Sarvesh Logsetty and
  • Afshin Raouf

17 September 2023

The scratch assay is an in vitro assay that allows for high-throughput quantification of wound closure by keratinocytes and fibroblasts with relative ease. However, this assay is amenable to experimental variables, which can result in false-positive...

  • Commentary
  • Open Access
17 Citations
74,452 Views
8 Pages

DNA impurities can impact the safety of genetically engineered pharmaceuticals; thus, a specific limit value must be set for them during marketing authorisation. This particularly applies to mRNA vaccines, as large quantities of DNA templates are use...

  • Article
  • Open Access
16 Citations
6,129 Views
16 Pages

The aim of this research is to define optimal conditions to improve the stability of gold and silver nanoparticles’ anti-zearalenone antibody conjugates for their utilisation in lateral flow immunochromatographic assay (LFIA). The Turkevich&nda...

  • Communication
  • Open Access
16 Citations
4,543 Views
12 Pages

This study investigated the suitability of X-ray fluorescence (XRF) analysis for routine multi-elemental composition analysis, checking its analytical capabilities by measuring a wide array of certified reference materials of soil and plant origin. A...

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Methods Protoc. - ISSN 2409-9279