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Special Issue "Single-Molecule Fluorescence Spectroscopy"

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Chemical Biology".

Deadline for manuscript submissions: 31 October 2018

Special Issue Editor

Guest Editor
Prof. Jörg Fitter

Physikalisches Institut (IA), RWTH Aachen, 52062 Aachen, Germany
Website | E-Mail
Interests: ligand-induced conformational changes; protein dynamics; protein folding; co-translational protein folding; molecular crowding; single-molecule FRET; neutron spectroscopy; conformational entropy; cell-free protein synthesis; GFP-based FRET sensors

Special Issue Information

Dear Colleagues,

Single-molecule measurements provide unique information on heterogeneous populations of molecules—a situation typically encountered in biological samples. Most important for life science applications, these measurements give access to the whole distribution of observables, rather than only an averaged mean value, which is usually obtained from bulk measurements. By using routine optical microscopy, the efficient collection and detection of fluorescence with careful minimization of background from impurities now enables the study of single molecules in complex or cytosolic environments.

Single-molecule fluorescence techniques have capabilities of probing structural and dynamical properties of macromolecular machineries via Förster resonance energy transfer (FRET), tracking single particles over micrometer distances, or by observing the rotational motion of multi-subunit systems. By applying these techniques, important discoveries continue to emerge in areas such as molecular motors, protein–DNA and protein–protein interactions, RNA activities, protein folding and dynamics, and enzymology. In order to tackle biologically meaningful questions, often not only methodical developments for efficient data acquisition and analysis are crucial, but also the development of strategies to attach one or more fluorescent dyes site-specifically to the biological macromolecules.

This Special Issue of Molecules covers all aspects related to the development and application of “Single-Molecule Fluorescence Spectroscopy”. Not only contributions dealing with techniques like FRET, fluorescence quenching, or single-particle tracking (SPT) are welcome, but also approaches which cover single-molecule properties in super-resolution microscopy, the combination of optical tweezer with fluorescence microscopy, or others. It is a pleasure to invite original research as well as review articles that describe and discuss technical developments in the fluorescence detection of single molecules as well as their applications.

Prof. Jörg Fitter
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • Single molecules
  • Static/dynamic heterogeneity
  • Fluorescence spectroscopy
  • Super-resolution microscopy
  • Proteins/protein-complexes
  • DNA/RNA complexes
  • Förster resonance energy transfer (FRET)
  • Single-particle tracking
  • Conformational dynamics
  • Subunit stoichiometry from single-molecule photo-bleaching

Published Papers

This special issue is now open for submission.
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