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Open AccessArticle

Single Molecule Fluorescence Spectroscopy of PSI Trimers from Arthrospira platensis: A Computational Approach

1
Prokhorov General Physics Institute of the Russian Academy of Sciences, 119991 Moscow, Russia
2
Bach Institute of Biochemistry of the Russian Academy of Sciences, 119071 Moscow, Russia
3
Belozersky Research Institute of Physico-Chemical Biology, Moscow State University, 119992 Moscow, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Jörg Fitter
Molecules 2019, 24(4), 822; https://doi.org/10.3390/molecules24040822
Received: 11 December 2018 / Revised: 20 February 2019 / Accepted: 21 February 2019 / Published: 25 February 2019
(This article belongs to the Special Issue Single-Molecule Fluorescence Spectroscopy)
Based on single molecule spectroscopy analysis and our preliminary theoretical studies, the linear and fluorescence spectra of the PSI trimer from Arthrospira platensis with different realizations of the static disorder were modeled at cryogenic temperature. Considering the previously calculated spectral density of chlorophyll, an exciton model for the PSI monomer and trimer including the red antenna states was developed taking into account the supposed similarity of PSI antenna structures from Thermosynechococcus e., Synechocystis sp. PCC6803, and Arthrospira platensis. The red Chls in the PSI monomer were assumed to be in the nearest proximity of the reaction center. The PSI trimer model allowed the simulation of experimentally measured zero phonon line distribution of the red states considering a weak electron-phonon coupling for the antenna exciton states. However, the broad absorption and fluorescence spectra of an individual emitter at 760 nm were calculated by adjusting the Huang-Rhys factors of the chlorophyll lower phonon modes assuming strong electron-phonon coupling. View Full-Text
Keywords: photosynthesis; PSI; long-wavelength antenna chlorophyll; energy transfer; single molecule spectroscopy; modified redfield theory; excitons photosynthesis; PSI; long-wavelength antenna chlorophyll; energy transfer; single molecule spectroscopy; modified redfield theory; excitons
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MDPI and ACS Style

Pishchalnikov, R.; Shubin, V.; Razjivin, A. Single Molecule Fluorescence Spectroscopy of PSI Trimers from Arthrospira platensis: A Computational Approach. Molecules 2019, 24, 822.

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