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Molecules 2018, 23(12), 3105; https://doi.org/10.3390/molecules23123105

Single-Molecule Studies on a FRET Biosensor: Lessons from a Comparison of Fluorescent Protein Equipped versus Dye-Labeled Species

1
Forschungszentrum Jülich, ICS-5, 52425 Jülich, Germany
2
RWTH Aachen University, I. Physikalisches Institut (IA), 52056 Aachen, Germany
3
Forschungszentrum Jülich, IBG-1, 52425 Jülich, Germany
*
Author to whom correspondence should be addressed.
Received: 31 October 2018 / Revised: 23 November 2018 / Accepted: 24 November 2018 / Published: 27 November 2018
(This article belongs to the Special Issue Single-Molecule Fluorescence Spectroscopy)
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Abstract

Bacterial periplasmic binding proteins (PBPs) undergo a pronounced ligand-induced conformational change which can be employed to monitor ligand concentrations. The most common strategy to take advantage of this conformational change for a biosensor design is to use a Förster resonance energy transfer (FRET) signal. This can be achieved by attaching either two fluorescent proteins (FPs) or two organic fluorescent dyes of different colors to the PBPs in order to obtain an optical readout signal which is closely related to the ligand concentration. In this study we compare a FP-equipped and a dye-labeled version of the glucose/galactose binding protein MglB at the single-molecule level. The comparison demonstrates that changes in the FRET signal upon glucose binding are more pronounced for the FP-equipped sensor construct as compared to the dye-labeled analog. Moreover, the FP-equipped sensor showed a strong increase of the FRET signal under crowding conditions whereas the dye-labeled sensor was not influenced by crowding. The choice of a labeling scheme should therefore be made depending on the application of a FRET-based sensor. View Full-Text
Keywords: Förster resonance energy transfer (FRET); single molecule studies; biosensor; fluorescent protein (FP); conformational change; hinge motion; ligand binding; glucose sensor Förster resonance energy transfer (FRET); single molecule studies; biosensor; fluorescent protein (FP); conformational change; hinge motion; ligand binding; glucose sensor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Höfig, H.; Cerminara, M.; Ritter, I.; Schöne, A.; Pohl, M.; Steffen, V.; Walter, J.; Vergara Dal Pont, I.; Katranidis, A.; Fitter, J. Single-Molecule Studies on a FRET Biosensor: Lessons from a Comparison of Fluorescent Protein Equipped versus Dye-Labeled Species. Molecules 2018, 23, 3105.

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