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Special Issue "Molecular Tools for Nucleic Acid Manipulation for Biological Intervention"

A special issue of Molecules (ISSN 1420-3049).

Deadline for manuscript submissions: 31 January 2019

Special Issue Editor

Guest Editor
Prof. Dr. Roger Strömberg

Department of Biosciences and Nutrition, Karolinska Institute, NEO, 14157 Huddinge, Sweden
Website | E-Mail
Interests: nucleic acid chemistry; oligonucleotides; peptides; oligonucleotide conjugates; oligonucleotide therapy; peptide conjugates; artificial nucleases; antimicrobial peptides; RNA therapy; gene therapy; affectors of innate immunity; treatment of infectious disease, degenerate disease, muscular dystrophy and cancer

Special Issue Information

Dear Colleagues,

Manipulation of nucleic acids and their activities has become indispensable in molecular biology and medicine. In addition, such manipulation is also the basis for emerging disease therapies, not least proven by recent FDA approvals of oligonucleotide therapy applications. The need for new developments in the field is crucial in order to obtain tools or therapeutics that are more efficient, selective or allow novel approaches, investigations or disease treatments. Here, chemistry will play a crucial role both in the understanding of mechanisms or in development of novel modifications, conjugates tools, etc.  

This Special Issue of Molecules is intended to encompass contributions from various approaches to influence biological action of nucleic acids, with a focus on chemistry. This includes methods for making, and studies on the action of modified oligonucleotides (ONs), ON conjugates and cleavers of phosphodiester bonds. The latter includes artificial (also oligonucleotide and peptide nucleic acid based ones) or modified nucleases and other phosphate ester transferring enzymes and nanoparticle based cleavers. In addition, delivery systems for ONs, as well as ON conjugates aimed at cell, tissue or subcellular delivery are a part of the topic.

Prof. Dr. Roger Strömberg
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • oligonucleotides
  • oligonucleotide therapy
  • oligonucleotide conjugates
  • phosphodiesterases
  • artificial nucleases
  • peptides
  • peptide conjugates
  • cellular uptake
  • drug delivery
  • nanoparticles

Published Papers (1 paper)

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Research

Open AccessArticle Two-Holder Strategy for Efficient and Selective Synthesis of Lk 1 ssDNA Catenane
Molecules 2018, 23(9), 2270; https://doi.org/10.3390/molecules23092270
Received: 23 August 2018 / Revised: 31 August 2018 / Accepted: 5 September 2018 / Published: 5 September 2018
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Abstract
DNA catenanes are characterized by their flexible and dynamic motions and have been regarded as one of the key players in sophisticated DNA-based molecular machines. There, the linking number (Lk) between adjacent interlocked rings is one of the most critical factors, since it
[...] Read more.
DNA catenanes are characterized by their flexible and dynamic motions and have been regarded as one of the key players in sophisticated DNA-based molecular machines. There, the linking number (Lk) between adjacent interlocked rings is one of the most critical factors, since it governs the feasibility of dynamic motions. However, there has been no established way to synthesize catenanes in which Lk is controlled to a predetermined value. This paper reports a new methodology to selectively synthesize Lk 1 catenanes composed of single-stranded DNA rings, in which these rings can most freely rotate each other due to minimal inter-ring interactions. To the mixture for the synthesis, two holder strands (oligonucleotides of 18–46 nt) were added, and the structure of the quasi-catenane intermediate was interlocked through Watson–Crick base pairings into a favorable conformation for Lk 1 catenation. The length of the complementary part between the two quasi-rings was kept at 10 bp or shorter. Under these steric constraints, two quasi-rings were cyclized with the use of T4 DNA ligase. By this simple procedure, the formation of undesired topoisomers (Lk ≥ 2) was almost completely inhibited, and Lk 1 catenane was selectively prepared in high yield up to 70 mole%. These Lk 1 catenanes have high potentials as dynamic parts for versatile DNA architectures. Full article
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