Special Issue "Synthesis and Applications of Oligonucleotide Conjugate II"
Deadline for manuscript submissions: 31 October 2020.
Interests: Kinetic studies on chemical models of ribonucleases and ribozymes; Synthesis and application of oligonucleotide conjugates; Pro-drug strategies for phosphoester drugs; Novel approaches for medium scale synthesis of oligonucleotides
Special Issues and Collections in MDPI journals
Special Issues and Collections in MDPI journals
Conjugates of oligonucleotides with an unnatural organic structure or a constituent of some biopolymer are widely used cell biology research tools. Conventional conjugate groups consist of reporter groups for sensitive detection of oligonucleotides both in vitro and in vivo, chemically or photochemically reactive groups for site-selective cleavage or cross-linking to the target sequence, intercalators for stabilization of double helices, and chelating agents for high affinity binding of metal ions. More recently, covalent conjugates have received increased interest as a means to improve the delivery of therapeutic oligonucleotides to specific cell types. A breakthrough in this field was the approval of a siRNA drug, givosiran, for clinical use. This is a trivalent N-acetylgalactosamine conjugate bearing the conjugate group at the 3’ terminus of the antisense strand. In addition, lipid, carbohydrate, peptide, aptamer, and some small molecule conjugates have shown promise. In particular, targeting with aptamers or peptides seems to be an increasingly popular approach. In many cases, conjugation through a biodegradable linker is desirable. Preparation of oligonucleotide conjugates is challenging. Synthesis on a single solid support is possible but often requires modifications to the conventional protocol of oligonucleotide synthesis. Alternatively, bio-orthogonal post-synthetic conjugation in solution may be applied.
The present Special Issue is aimed at gathering new synthetic methodologies of oligonucleotide conjugates, as well as their novel applications.
Prof. Harri Lönnberg
Prof. Dr. Roger Strömberg
Manuscript Submission Information
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- therapeutic oligonucleotides
- Synthesis and Applications of Oligonucleotide Conjugates in Molecules (11 articles)
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Contribution of antisense oligonucleotide and chemotherapy combination to cancer treatment
Authors: Asuman Bozkır
Affiliation: Ankara Üniversity,Faculty of Pharmacy, Department of Pharmaceutical Technology, 06560 Yenimahalle/ANKARA
Title: Highly multiplexed single-cell in situ RNA and DNA analysis by consecutive hybridization
Authors: Jia Guo
Affiliation: School of Molecular Sciences, Arizona State University
Abstract: Limitations on the number of RNA species and genomic loci that can be quantified in single cells in situ impede advances in our deep understanding of normal cell physiology and disease pathogenesis. Here we present a method for highly multiplexed single cell in situ RNA and DNA analysis by consecutive fluorescence in situ hybridization (C-FISH). In this method, individual transcripts or genomic loci are visualized as fluorescent spots with fixed locations in individual cells. In each cycle of C-FISH, fluorescently labeled oligonucleotides hybridize to the probe used in the previous cycle, and also introduce multiple binding sites for the probe of the following cycle. Through consecutive cycles of probe hybridization, fluorescence imaging and photobleaching, different RNA species or genomic loci can be identified as fluorescent spots with unique color sequences. Applying this approach, we demonstrate that with 2 fluorophores and 16 cycles of C-FISH, different transcripts or genomic loci are unambiguously identified in individual cells with close to “0” raw data error rate. These results suggest all the varied color sequences (216= 65,536) can be applied to identify distinct RNA species or genomic loci, which allows the transcriptome- or genome-wide single cell in situ analysis. This highly multiplexed single cell in situ RNA and DNA analysis technology will have wide applications in signaling network analysis, 3D genome architecture studies, molecular diagnosis and cellular targeted therapies