E-Mail Alert

Add your e-mail address to receive forthcoming issues of this journal:

Journal Browser

Journal Browser

Special Issue "Microfluidic Tools for High-Throughput Screening"

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: 31 October 2019

Special Issue Editor

Guest Editor
Prof. R. Michael Van Dam

Crump Institute for Molecular Imaging, Department of Molecular & Medical Pharmacology, and Department of Bioengineering, University of California, Los Angeles, USA
Website | E-Mail
Interests: microfluidics; radiochemical synthesis; radiochemical analysis; automation; molecular imaging

Special Issue Information

Dear Colleagues,

There has been tremendous progress over the last several years in the development and commercialization of microfluidic technologies for high-throughput screening (HTS) and library generation. Across a wide range of applications, microfluidic HTS has numerous advantages over conventional tools, including reduced sample and reagent volumes, improved measurement sensitivity, higher density of assays and reagent/sample storage, and implementation of multi-step assays in simple multifunctional microfluidic devices.

HTS has been implemented in diverse types of microfluidic devices including (1) analogs of microplates in which assays are performed in an array of tiny wells or chambers, (2) array-based systems in which samples/reagents are flowed over an array of immobilized or trapped reagents/samples to perform assays, or (3) droplet-in-oil based systems in which assays are performed in a series of isolated droplets separated by an oil stream. Depending on the assay, the devices may manipulate single cells, 2D or 3D cell cultures, tissues, spheroids, small organisms, or biomolecules (e.g., proteins, nucleic acids, peptides), etc. In order to identify “hits”, readout may rely on fluorescence, luminescence, nanoparticle binding, electrical impedance, radioactivity, microscopy (e.g., cell morphology, crystal formation), size, PCR amplification and a multitude of other outputs.

For this Special Issue, we invite original research or review papers that focus on the many current and emerging microfluidic platforms for high-throughput screening, as well as their diverse applications.

Prof. R. Michael van Dam
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access bimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microfluidics
  • micro total analysis systems
  • lab on a chip
  • combinatorial synthesis
  • high-throughput screening
  • parallelism

Published Papers (1 paper)

View options order results:
result details:
Displaying articles 1-1
Export citation of selected articles as:

Research

Open AccessArticle A Microfluidic Spheroid Culture Device with a Concentration Gradient Generator for High-Throughput Screening of Drug Efficacy
Molecules 2018, 23(12), 3355; https://doi.org/10.3390/molecules23123355
Received: 27 November 2018 / Revised: 17 December 2018 / Accepted: 17 December 2018 / Published: 18 December 2018
PDF Full-text (2360 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Three-dimensional (3D) cell culture is considered more clinically relevant in mimicking the structural and physiological conditions of tumors in vivo compared to two-dimensional cell cultures. In recent years, high-throughput screening (HTS) in 3D cell arrays has been extensively used for drug discovery because
[...] Read more.
Three-dimensional (3D) cell culture is considered more clinically relevant in mimicking the structural and physiological conditions of tumors in vivo compared to two-dimensional cell cultures. In recent years, high-throughput screening (HTS) in 3D cell arrays has been extensively used for drug discovery because of its usability and applicability. Herein, we developed a microfluidic spheroid culture device (μFSCD) with a concentration gradient generator (CGG) that enabled cells to form spheroids and grow in the presence of cancer drug gradients. The device is composed of concave microwells with several serpentine micro-channels which generate a concentration gradient. Once the colon cancer cells (HCT116) formed a single spheroid (approximately 120 μm in diameter) in each microwell, spheroids were perfused in the presence of the cancer drug gradient irinotecan for three days. The number of spheroids, roundness, and cell viability, were inversely proportional to the drug concentration. These results suggest that the μFSCD with a CGG has the potential to become an HTS platform for screening the efficacy of cancer drugs. Full article
(This article belongs to the Special Issue Microfluidic Tools for High-Throughput Screening)
Figures

Graphical abstract

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top